Restriction map for
pFN21AB6417
(Circular) (Six-base) MAPSORT of: pFN21AB6417.seq Check: 5156 from: 1 to: 5632
pFN21AB6417 5632 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2445 2975 1937
Size: 508 530 4594
Fragments arranged by size:
4594 530 508
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2630 3695 5079 5347 1632
Size: 998 1065 1384 268 1917
Fragments arranged by size:
1917 1384 1065 998 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2756 2941 3167 3319 5428 1493
Size: 1263 185 226 152 2109 1697
Fragments arranged by size:
2109 1697 1263 226 185 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2312 2957 1870
Size: 73 369 645 4545
Fragments arranged by size:
4545 645 369 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2133 2291 2645 2688 2780
Size: 325 217 627 346 158 354 43 92
Cuts at: 2780 2953 4408 618
Size: 173 1455 1842
Fragments arranged by size:
1842 1455 627 354 346 325 217 173
158 92 43
BanII G_rGCy'C
Cuts at: 1391 2676 2951 1391
Size: 1285 275 4072
Fragments arranged by size:
4072 1285 275
BbeI G_GCGC'C
Cuts at: 2137 2295 2137
Size: 158 5474
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BbvCI CC'TCA_GC
Cuts at: 2022 2022
Size: 5632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3823 3857 1903
Size: 34 50 34 1802 34 3678
Fragments arranged by size:
3678 1802 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2785 3693 4849 1790
Size: 995 908 1156 2573
Fragments arranged by size:
2573 1156 995 908
BmgBI CAC'GTC
Cuts at: 1318 2344 1318
Size: 1026 4606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2013 2255 2372 2760 4292 1239
Size: 774 242 117 388 1532 2579
Fragments arranged by size:
2579 1532 774 388 242 117
Bpu10I CC'TnA_GC
Cuts at: 2022 2517 2022
Size: 495 5137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2089 4295 882
Size: 617 590 2206 2219
Fragments arranged by size:
2219 2206 617 590
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 2804 5626 2804
Size: 2822 2810
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2134 2271 2284 2292
Size: 53 83 186 936 601 137 13 8
Cuts at: 2292 3439 3821 4456 275
Size: 1147 382 635 1451
Fragments arranged by size:
1451 1147 936 635 601 382 186 137
83 53 13 8
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2077 2213 2387 2426 1186
Size: 891 136 174 39 4392
Fragments arranged by size:
4392 891 174 136 39
BseYI C'CCAG_C
Cuts at: 2329 2356 2363 2549 2837 4835 5331 2329
Size: 27 7 186 288 1998 496 2630
Fragments arranged by size:
2630 1998 496 288 186 27 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2588 2866 1163
Size: 573 852 278 3929
Fragments arranged by size:
3929 852 573 278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 2178 3082 3843 4871 1540
Size: 275 186 16 161 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186 161 16
BsiHKAI G_wGCw'C
Cuts at: 2676 3693 3778 4849 2676
Size: 1017 85 1071 3459
Fragments arranged by size:
3459 1071 1017 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2676 2785 2951 3693 3778 4849 1391
Size: 399 886 109 166 742 85 1071 2174
Fragments arranged by size:
2174 1071 886 742 399 166 109 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2733 2987 844
Size: 1889 254 3489
Fragments arranged by size:
3489 1889 254
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 4127 4301 65
Size: 1183 1578 1301 174 1396
Fragments arranged by size:
1578 1396 1301 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2223 2962 3329 3714 3731 5183 5269 5281
Size: 251 739 367 385 17 1452 86 12
Cuts at: 5281 5627 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 739 385 367 346 251 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2846 2846
Size: 5632
BtgI C'CryG_G
Cuts at: 513 1065 2043 2459 513
Size: 552 978 416 3686
Fragments arranged by size:
3686 978 552 416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2646 487
Size: 683 56 1420 3473
Fragments arranged by size:
3473 1420 683 56
BtsI GCAGTG_nn'
Cuts at: 2458 3148 3942 3962 2458
Size: 690 794 20 4128
Fragments arranged by size:
4128 794 690 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2453 2633 4639 809
Size: 507 1137 180 2006 1802
Fragments arranged by size:
2006 1802 1137 507 180
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2436 3079
Size: 54 1425 50 275 18 345 261 643
Cuts at: 3079 3968 5597 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 643 345 275 261 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2175 3079 1537
Size: 275 363 904 4090
Fragments arranged by size:
4090 904 363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2210 4219 4593 4739 387
Size: 1064 138 621 2009 374 146 1280
Fragments arranged by size:
2009 1280 1064 621 374 146 138
EcoICRI GAG'CTC
Cuts at: 2674 2674
Size: 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2255 2372 2630 2760 3695 4292
Size: 393 381 242 117 258 130 935 597
Cuts at: 4292 5079 5347 1239
Size: 787 268 1524
Fragments arranged by size:
1524 935 787 597 393 381 268 258
242 130 117
EcoNI CCTnn'n_nnAGG
Cuts at: 2912 2912
Size: 5632
EcoO109I rG'GnC_Cy
Cuts at: 2057 2360 2057
Size: 303 5329
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2137 2295 4779 1678
Size: 459 158 2484 2531
Fragments arranged by size:
2531 2484 459 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2587 2619 4273 4305 4347
Size: 32 135 32 1015 32 1654 32 42
Cuts at: 4347 4379 1373
Size: 32 2626
Fragments arranged by size:
2626 1654 1015 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2976 3222 669
Size: 988 281 1038 246 3079
Fragments arranged by size:
3079 1038 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KasI G'GCGC_C
Cuts at: 2133 2291 2133
Size: 158 5474
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 3590 3949 4108 518
Size: 648 960 1464 359 159 2042
Fragments arranged by size:
2042 1464 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2441 3048 3725 4873 5118 1606
Size: 835 607 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 607 245
NarI GG'CG_CC
Cuts at: 2134 2292 2134
Size: 158 5474
NcoI C'CATG_G
Cuts at: 513 1065 2043 513
Size: 552 978 4102
Fragments arranged by size:
4102 978 552
NdeI CA'TA_TG
Cuts at: 387 2511 387
Size: 2124 3508
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2125 2990 4535 1132
Size: 97 896 865 1545 2229
Fragments arranged by size:
2229 1545 896 865 97
PasI CC'CwG_GG
Cuts at: 1348 2503 1348
Size: 1155 4477
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2892 1108
Size: 1784 3848
PfoI T'CCnGG_A
Cuts at: 2029 2029
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2360 2057
Size: 303 5329
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspXI vC'TCGA_Gb
Cuts at: 2312 2312
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2053 2730 2984 830
Size: 1223 677 254 3478
Fragments arranged by size:
3478 1223 677 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
SacI G_AGCT'C
Cuts at: 2676 2676
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SanDI GG'GwC_CC
Cuts at: 2360 2360
Size: 5632
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 2216 3880 5592 2216
Size: 1664 1712 2256
Fragments arranged by size:
2256 1712 1664
SfcI C'TryA_G
Cuts at: 826 1045 2049 2298 2442 2726 2980 4115 4796
Size: 219 1004 249 144 284 254 1135 681
Cuts at: 4796 4987 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1004 681 284 254 249 219
191 144
SfoI GGC'GCC
Cuts at: 2135 2293 2135
Size: 158 5474
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 3736 4637 4899 820
Size: 197 926 369 1424 901 262 1553
Fragments arranged by size:
1553 1424 926 901 369 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 2918 1517
Size: 1401 4231
StyI C'CwwG_G
Cuts at: 513 1065 2043 2913 3071 513
Size: 552 978 870 158 3074
Fragments arranged by size:
3074 978 870 552 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 2811 3878
Size: 274 80 33 51 1443 237 597 1067
Cuts at: 3878 5590 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1067 597 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 2312 1943
Size: 369 5263
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 2831 3761 2831
Size: 930 4702
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI
SwaI XcmI