Restriction map for
pFN21AB6415
(Circular) (Six-base) MAPSORT of: pFN21AB6415.seq Check: 8920 from: 1 to: 5629
pFN21AB6415 5629 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3439 278
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2430 2972 1937
Size: 493 542 4594
Fragments arranged by size:
4594 542 493
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 3692 5076 5344 1632
Size: 747 1313 1384 268 1917
Fragments arranged by size:
1917 1384 1313 747 268
AfeI AGC'GCT
Cuts at: 2843 2843
Size: 5629
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 2202 4358 2202
Size: 2156 3473
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2541 2573 2541
Size: 32 5597
AlwNI CAG_nnn'CTG
Cuts at: 2731 3372 4944 2731
Size: 641 1572 3416
Fragments arranged by size:
3416 1572 641
ApaI G_GGCC'C
Cuts at: 2096 2096
Size: 5629
ApaLI G'TGCA_C
Cuts at: 2571 3686 4842 2571
Size: 1115 1156 3358
Fragments arranged by size:
3358 1156 1115
ApoI r'AATT_y
Cuts at: 1493 2938 3164 3316 5425 1493
Size: 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2161 2222 2954 1870
Size: 73 218 61 732 4545
Fragments arranged by size:
4545 732 218 73 61
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2299 2804 2950 4405
Size: 325 217 627 378 134 505 146 1455
Cuts at: 4405 618
Size: 1842
Fragments arranged by size:
1842 1455 627 505 378 325 217 146
134
BanII G_rGCy'C
Cuts at: 1391 2096 2948 1391
Size: 705 852 4072
Fragments arranged by size:
4072 852 705
BbeI G_GCGC'C
Cuts at: 2303 2303
Size: 5629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 1903
Size: 34 1883 34 3678
Fragments arranged by size:
3678 1883 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3521 4731 3521
Size: 1210 4419
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2076 2174 4240
Size: 107 122 71 631 605 404 98 2066
Cuts at: 4240 136
Size: 1525
Fragments arranged by size:
2066 1525 631 605 404 122 107 98
71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 2243 3046 2243
Size: 803 4826
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2170 2379 2575 2595 3690 4846 1790
Size: 306 74 209 196 20 1095 1156 2573
Fragments arranged by size:
2573 1156 1095 306 209 196 74 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3360 4318 442
Size: 416 53 83 399 24 1943 958 1753
Fragments arranged by size:
1943 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4289 1239
Size: 3050 2579
Bpu10I CC'TnA_GC
Cuts at: 2248 2248
Size: 5629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2647 4292 882
Size: 617 1148 1645 2219
Fragments arranged by size:
2219 1645 1148 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2300 3436 3818 4453
Size: 53 83 186 936 767 1136 382 635
Cuts at: 4453 275
Size: 1451
Fragments arranged by size:
1451 1136 936 767 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2032 2062 1178
Size: 30 165 30 629 30 4745
Fragments arranged by size:
4745 629 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5629
BseYI C'CCAG_C
Cuts at: 2293 4832 5328 2293
Size: 2539 496 2594
Fragments arranged by size:
2594 2539 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 1163
Size: 573 357 4699
Fragments arranged by size:
4699 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2181 2187 3079 3840 4868 1540
Size: 275 186 180 6 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186 180 6
BsiHKAI G_wGCw'C
Cuts at: 2575 3690 3775 4846 2575
Size: 1115 85 1071 3358
Fragments arranged by size:
3358 1115 1071 85
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2170 2379 2575 2595 2948 3690
Size: 399 306 74 209 196 20 353 742
Cuts at: 3690 3775 4846 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 353 306 209 196
85 74 20
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 3518 5248 3518
Size: 1730 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2984 844
Size: 1307 833 3489
Fragments arranged by size:
3489 1307 833
BsrBI CCG'CTC
Cuts at: 1394 1823 2033 3516 1394
Size: 429 210 1483 3507
Fragments arranged by size:
3507 1483 429 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 2708 4124 4298 65
Size: 1183 1460 1416 174 1396
Fragments arranged by size:
1460 1416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 4273 4460 1700
Size: 110 315 2148 187 2869
Fragments arranged by size:
2869 2148 315 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 2327 1925
Size: 402 5227
BssSI C'ACGA_G
Cuts at: 2603 2634 3689 4701 2603
Size: 31 1055 1012 3531
Fragments arranged by size:
3531 1055 1012 31
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 2401
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2959 3326 3711 3728 5180 5266 5278 5624
Size: 987 367 385 17 1452 86 12 346
Cuts at: 5624 1972
Size: 1977
Fragments arranged by size:
1977 1452 987 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2044 2181 2484 513
Size: 552 979 137 303 3658
Fragments arranged by size:
3658 979 552 303 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 2473 3145 3939 3959 2473
Size: 672 794 20 4143
Fragments arranged by size:
4143 794 672 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2184 2470
Size: 54 1425 50 275 18 348 6 286
Cuts at: 2470 2481 3076 3965 5594 8
Size: 11 595 889 1629 43
Fragments arranged by size:
1629 1425 889 595 348 286 275 54
50 43 18 11 6
EagI C'GGCC_G
Cuts at: 1537 1812 2178 2184 3076 1537
Size: 275 366 6 892 4090
Fragments arranged by size:
4090 892 366 275 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 3559 1353
Size: 1053 1153 3423
Fragments arranged by size:
3423 1153 1053
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 3692 4289 5076 5344 1239
Size: 393 747 1313 597 787 268 1524
Fragments arranged by size:
1524 1313 787 747 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2092 2093 2196 2204 2092
Size: 1 103 8 5517
Fragments arranged by size:
5517 103 8 1
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2863 2902 2548
Size: 315 39 5275
Fragments arranged by size:
5275 315 39
FspI TGC'GCA
Cuts at: 2765 4135 2765
Size: 1370 4259
HaeII r_GCGC'y
Cuts at: 1678 2071 2156 2303 2322 2732 2845 4776 1678
Size: 393 85 147 19 410 113 1931 2531
Fragments arranged by size:
2531 1931 410 393 147 113 85 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2188 2220 4270 4302 4344
Size: 32 135 32 616 32 2050 32 42
Cuts at: 4344 4376 1373
Size: 32 2626
Fragments arranged by size:
2626 2050 616 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KasI G'GCGC_C
Cuts at: 2299 2299
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4743 4927 1305
Size: 3438 184 2007
Fragments arranged by size:
3438 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2483 10
Size: 54 1425 343 651 3156
Fragments arranged by size:
3156 1425 651 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2382 2399 2483 2603 2718 3587
Size: 648 960 256 17 84 120 115 869
Cuts at: 3587 3946 4105 518
Size: 359 159 2042
Fragments arranged by size:
2042 960 869 648 359 256 159 120
115 84 17
MspA1I CmG'CkG
Cuts at: 1606 2028 2046 2107 2183 2460 2563 2705 3045
Size: 422 18 61 76 277 103 142 340
Cuts at: 3045 3722 4870 5115 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 422 340 277 245 142
103 76 61 18
NarI GG'CG_CC
Cuts at: 2300 2300
Size: 5629
NcoI C'CATG_G
Cuts at: 513 1065 2484 513
Size: 552 1419 3658
Fragments arranged by size:
3658 1419 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 2178 3076 2178
Size: 898 4731
NspI r_CATG'y
Cuts at: 1132 1229 2125 2987 4532 1132
Size: 97 896 862 1545 2229
Fragments arranged by size:
2229 1545 896 862 97
PasI CC'CwG_GG
Cuts at: 1348 2088 2200 1348
Size: 740 112 4777
Fragments arranged by size:
4777 740 112
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 1108
Size: 1371 4258
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2196 2204 2196
Size: 8 5621
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5629
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PspOMI G'GGCC_C
Cuts at: 2092 2092
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2148 2981 830
Size: 1318 833 3478
Fragments arranged by size:
3478 1318 833
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2563 2563
Size: 5629
SacII CC_GC'GG
Cuts at: 2047 2184 2047
Size: 137 5492
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SanDI GG'GwC_CC
Cuts at: 2196 2204 2196
Size: 8 5621
SbfI CC_TGCA'GG
Cuts at: 2148 2981 2148
Size: 833 4796
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SexAI A'CCwGG_T
Cuts at: 2622 2622
Size: 5629
SfcI C'TryA_G
Cuts at: 826 1045 2144 2977 4112 4793 4984 826
Size: 219 1099 833 1135 681 191 1471
Fragments arranged by size:
1471 1135 1099 833 681 219 191
SfoI GGC'GCC
Cuts at: 2301 2301
Size: 5629
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2163 2956 1872
Size: 291 793 4545
Fragments arranged by size:
4545 793 291
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2125 2987 1132
Size: 993 862 3774
Fragments arranged by size:
3774 993 862
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 2543 1517
Size: 1026 4603
StyI C'CwwG_G
Cuts at: 513 1065 2484 2824 3068 513
Size: 552 1419 340 244 3074
Fragments arranged by size:
3074 1419 552 340 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3656 3673 3826 4011 4456 325
Size: 1255 2076 17 153 185 445 1498
Fragments arranged by size:
2076 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 3875 5587
Size: 274 80 33 51 1443 531 1367 1712
Cuts at: 5587 96
Size: 138
Fragments arranged by size:
1712 1443 1367 531 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2032 2064 5589 5621 2032
Size: 32 3525 32 2040
Fragments arranged by size:
3525 2040 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2602 2602
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2161 2954 1870
Size: 291 793 4545
Fragments arranged by size:
4545 793 291
XmnI GAAnn'nnTTC
Cuts at: 2843 3758 2843
Size: 915 4714
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3437 276
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SapI SfiI SrfI SwaI