(Circular) (Six-base) MAPSORT of: pFN21AB6414.seq Check: 9460 from: 1 to: 5710 pFN21AB6414 5710 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2235 2247 2691 3773 5157 5425 1632 Size: 603 12 444 1082 1384 268 1917 Fragments arranged by size: 1917 1384 1082 603 444 268 12 AfeI AGC'GCT Cuts at: 2377 2377 Size: 5710 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 3453 5025 3453 Size: 1572 4138 ApaLI G'TGCA_C Cuts at: 2142 2310 3767 4923 2142 Size: 168 1457 1156 2929 Fragments arranged by size: 2929 1457 1156 168 ApoI r'AATT_y Cuts at: 1493 2281 2505 2802 3019 3245 3397 5506 1493 Size: 788 224 297 217 226 152 2109 1697 Fragments arranged by size: 2109 1697 788 297 226 224 217 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 3035 1870 Size: 73 1092 4545 Fragments arranged by size: 4545 1092 73 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3031 4486 618 Size: 325 217 627 335 909 1455 1842 Fragments arranged by size: 1842 1455 909 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 2134 2631 3029 1391 Size: 743 497 398 4072 Fragments arranged by size: 4072 743 497 398 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BbvCI CC'TCA_GC Cuts at: 2022 2607 2913 2022 Size: 585 306 4819 Fragments arranged by size: 4819 585 306 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BfrBI ATG'CAT Cuts at: 2351 2351 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4321 136 Size: 107 122 71 631 605 337 2312 1525 Fragments arranged by size: 2312 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2398 5705 2398 Size: 3307 2403 BlpI GC'TnA_GC Cuts at: 2056 3127 2056 Size: 1071 4639 Bme1580I G_kGCm'C Cuts at: 1790 2125 2146 2314 3771 4927 1790 Size: 335 21 168 1457 1156 2573 Fragments arranged by size: 2573 1457 1156 335 168 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2071 2071 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2643 4370 1239 Size: 1404 1727 2579 Fragments arranged by size: 2579 1727 1404 Bpu10I CC'TnA_GC Cuts at: 2022 2607 2913 2022 Size: 585 306 4819 Fragments arranged by size: 4819 585 306 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2172 2451 3835 4700 4998 2172 Size: 279 1384 865 298 2884 Fragments arranged by size: 2884 1384 865 298 279 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3517 3899 4534 275 Size: 53 83 186 936 1984 382 635 1451 Fragments arranged by size: 1984 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2994 3024 1178 Size: 30 165 30 1591 30 3864 Fragments arranged by size: 3864 1591 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2087 2454 3015 1186 Size: 874 27 367 561 3881 Fragments arranged by size: 3881 874 561 367 27 BseYI C'CCAG_C Cuts at: 2372 4913 5409 2372 Size: 2541 496 2673 Fragments arranged by size: 2673 2541 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2299 3160 3921 4949 1540 Size: 275 186 39 259 861 761 1028 2301 Fragments arranged by size: 2301 1028 861 761 275 259 186 39 BsiHKAI G_wGCw'C Cuts at: 2146 2314 2631 3771 3856 4927 2146 Size: 168 317 1140 85 1071 2929 Fragments arranged by size: 2929 1140 1071 317 168 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2125 2134 2146 2314 2631 3029 3771 Size: 399 335 9 12 168 317 398 742 Cuts at: 3771 3856 4927 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 398 335 317 168 85 12 9 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3065 844 Size: 2221 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2398 3040 3407 3792 3809 5261 5347 5359 Size: 426 642 367 385 17 1452 86 12 Cuts at: 5359 5705 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 642 426 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 2126 513 Size: 552 939 36 86 4097 Fragments arranged by size: 4097 939 552 86 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DraIII CAC_nnn'GTG Cuts at: 2141 2480 2141 Size: 339 5371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2900 3157 Size: 54 1425 50 275 18 207 863 257 Cuts at: 3157 4046 5675 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 863 275 257 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2037 3157 1537 Size: 275 225 1120 4090 Fragments arranged by size: 4090 1120 275 225 EarI CTCTTCn'nnn_ Cuts at: 1353 2553 3640 1353 Size: 1200 1087 3423 Fragments arranged by size: 3423 1200 1087 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2629 2629 Size: 5710 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2247 2643 2691 3773 4370 5157 Size: 393 603 12 396 48 1082 597 787 Cuts at: 5157 5425 1239 Size: 268 1524 Fragments arranged by size: 1524 1082 787 603 597 396 393 268 48 12 EcoRI G'AATT_C Cuts at: 2281 3019 3397 2281 Size: 738 378 4594 Fragments arranged by size: 4594 738 378 EcoRV GAT'ATC Cuts at: 2545 2545 Size: 5710 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2379 4857 1678 Size: 701 2478 2531 Fragments arranged by size: 2531 2478 701 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2559 2566 2591 4351 Size: 32 135 32 962 25 7 25 1760 Cuts at: 4351 4383 4425 4457 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1760 962 135 42 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2328 3054 3300 669 Size: 988 281 390 726 246 3079 Fragments arranged by size: 3079 988 726 390 281 246 HindIII A'AGCT_T Cuts at: 748 2739 2886 748 Size: 1991 147 3572 Fragments arranged by size: 3572 1991 147 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2187 4824 5008 1305 Size: 882 2637 184 2007 Fragments arranged by size: 2637 2007 882 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2902 10 Size: 54 1425 343 1070 2818 Fragments arranged by size: 2818 1425 1070 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3668 4027 4186 518 Size: 648 2502 359 159 2042 Fragments arranged by size: 2502 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2917 3126 3803 4951 5196 1606 Size: 424 887 209 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 424 245 209 NcoI C'CATG_G Cuts at: 513 1065 2004 2126 513 Size: 552 939 122 4097 Fragments arranged by size: 4097 939 552 122 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NsiI A_TGCA'T Cuts at: 2353 2353 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2318 2351 2446 3068 4613 1132 Size: 97 1089 33 95 622 1545 2229 Fragments arranged by size: 2229 1545 1089 622 97 95 33 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 2480 1108 Size: 1372 4338 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 3062 830 Size: 2232 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2030 2917 2030 Size: 887 4823 SacI G_AGCT'C Cuts at: 2631 2631 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 2824 3958 5670 2824 Size: 1134 1712 2864 Fragments arranged by size: 2864 1712 1134 SfcI C'TryA_G Cuts at: 826 1045 2031 3058 4193 4874 5065 826 Size: 219 986 1027 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 986 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2187 2466 3814 4715 4977 820 Size: 197 926 244 279 1348 901 262 1553 Fragments arranged by size: 1553 1348 926 901 279 262 244 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 2446 3068 1132 Size: 1314 622 3774 Fragments arranged by size: 3774 1314 622 SspI AAT'ATT Cuts at: 5 52 2524 3634 5 Size: 47 2472 1110 2081 Fragments arranged by size: 2472 2081 1110 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2126 3149 513 Size: 552 939 122 1023 3074 Fragments arranged by size: 3074 1023 939 552 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2313 3737 3754 3907 4092 4537 325 Size: 1255 733 1424 17 153 185 445 1498 Fragments arranged by size: 1498 1424 1255 733 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2822 3956 5668 Size: 274 80 33 51 1443 845 1134 1712 Cuts at: 5668 96 Size: 138 Fragments arranged by size: 1712 1443 1134 845 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 2220 2508 2562 2868 3839 2220 Size: 288 54 306 971 4091 Fragments arranged by size: 4091 971 306 288 54 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI