Restriction map for
pFN21AB6414
(Circular) (Six-base) MAPSORT of: pFN21AB6414.seq Check: 9460 from: 1 to: 5710
pFN21AB6414 5710 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2235 2247 2691 3773 5157 5425 1632
Size: 603 12 444 1082 1384 268 1917
Fragments arranged by size:
1917 1384 1082 603 444 268 12
AfeI AGC'GCT
Cuts at: 2377 2377
Size: 5710
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 3453 5025 3453
Size: 1572 4138
ApaLI G'TGCA_C
Cuts at: 2142 2310 3767 4923 2142
Size: 168 1457 1156 2929
Fragments arranged by size:
2929 1457 1156 168
ApoI r'AATT_y
Cuts at: 1493 2281 2505 2802 3019 3245 3397 5506 1493
Size: 788 224 297 217 226 152 2109 1697
Fragments arranged by size:
2109 1697 788 297 226 224 217 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3031 4486 618
Size: 325 217 627 335 909 1455 1842
Fragments arranged by size:
1842 1455 909 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2134 2631 3029 1391
Size: 743 497 398 4072
Fragments arranged by size:
4072 743 497 398
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BbvCI CC'TCA_GC
Cuts at: 2022 2607 2913 2022
Size: 585 306 4819
Fragments arranged by size:
4819 585 306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BfrBI ATG'CAT
Cuts at: 2351 2351
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4321 136
Size: 107 122 71 631 605 337 2312 1525
Fragments arranged by size:
2312 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2398 5705 2398
Size: 3307 2403
BlpI GC'TnA_GC
Cuts at: 2056 3127 2056
Size: 1071 4639
Bme1580I G_kGCm'C
Cuts at: 1790 2125 2146 2314 3771 4927 1790
Size: 335 21 168 1457 1156 2573
Fragments arranged by size:
2573 1457 1156 335 168 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2643 4370 1239
Size: 1404 1727 2579
Fragments arranged by size:
2579 1727 1404
Bpu10I CC'TnA_GC
Cuts at: 2022 2607 2913 2022
Size: 585 306 4819
Fragments arranged by size:
4819 585 306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2172 2451 3835 4700 4998 2172
Size: 279 1384 865 298 2884
Fragments arranged by size:
2884 1384 865 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2994 3024 1178
Size: 30 165 30 1591 30 3864
Fragments arranged by size:
3864 1591 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2087 2454 3015 1186
Size: 874 27 367 561 3881
Fragments arranged by size:
3881 874 561 367 27
BseYI C'CCAG_C
Cuts at: 2372 4913 5409 2372
Size: 2541 496 2673
Fragments arranged by size:
2673 2541 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2299 3160 3921 4949 1540
Size: 275 186 39 259 861 761 1028 2301
Fragments arranged by size:
2301 1028 861 761 275 259 186 39
BsiHKAI G_wGCw'C
Cuts at: 2146 2314 2631 3771 3856 4927 2146
Size: 168 317 1140 85 1071 2929
Fragments arranged by size:
2929 1140 1071 317 168 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2125 2134 2146 2314 2631 3029 3771
Size: 399 335 9 12 168 317 398 742
Cuts at: 3771 3856 4927 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 335 317 168
85 12 9
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3065 844
Size: 2221 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2398 3040 3407 3792 3809 5261 5347 5359
Size: 426 642 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 642 426 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2126 513
Size: 552 939 36 86 4097
Fragments arranged by size:
4097 939 552 86 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DraIII CAC_nnn'GTG
Cuts at: 2141 2480 2141
Size: 339 5371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2900 3157
Size: 54 1425 50 275 18 207 863 257
Cuts at: 3157 4046 5675 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 863 275 257 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2037 3157 1537
Size: 275 225 1120 4090
Fragments arranged by size:
4090 1120 275 225
EarI CTCTTCn'nnn_
Cuts at: 1353 2553 3640 1353
Size: 1200 1087 3423
Fragments arranged by size:
3423 1200 1087
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2629 2629
Size: 5710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2247 2643 2691 3773 4370 5157
Size: 393 603 12 396 48 1082 597 787
Cuts at: 5157 5425 1239
Size: 268 1524
Fragments arranged by size:
1524 1082 787 603 597 396 393 268
48 12
EcoRI G'AATT_C
Cuts at: 2281 3019 3397 2281
Size: 738 378 4594
Fragments arranged by size:
4594 738 378
EcoRV GAT'ATC
Cuts at: 2545 2545
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2379 4857 1678
Size: 701 2478 2531
Fragments arranged by size:
2531 2478 701
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2559 2566 2591 4351
Size: 32 135 32 962 25 7 25 1760
Cuts at: 4351 4383 4425 4457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1760 962 135 42 32 32 32
32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2328 3054 3300 669
Size: 988 281 390 726 246 3079
Fragments arranged by size:
3079 988 726 390 281 246
HindIII A'AGCT_T
Cuts at: 748 2739 2886 748
Size: 1991 147 3572
Fragments arranged by size:
3572 1991 147
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 4824 5008 1305
Size: 882 2637 184 2007
Fragments arranged by size:
2637 2007 882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2902 10
Size: 54 1425 343 1070 2818
Fragments arranged by size:
2818 1425 1070 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3668 4027 4186 518
Size: 648 2502 359 159 2042
Fragments arranged by size:
2502 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2917 3126 3803 4951 5196 1606
Size: 424 887 209 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 424 245 209
NcoI C'CATG_G
Cuts at: 513 1065 2004 2126 513
Size: 552 939 122 4097
Fragments arranged by size:
4097 939 552 122
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NsiI A_TGCA'T
Cuts at: 2353 2353
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2318 2351 2446 3068 4613 1132
Size: 97 1089 33 95 622 1545 2229
Fragments arranged by size:
2229 1545 1089 622 97 95 33
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2480 1108
Size: 1372 4338
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2030 2917 2030
Size: 887 4823
SacI G_AGCT'C
Cuts at: 2631 2631
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 2824 3958 5670 2824
Size: 1134 1712 2864
Fragments arranged by size:
2864 1712 1134
SfcI C'TryA_G
Cuts at: 826 1045 2031 3058 4193 4874 5065 826
Size: 219 986 1027 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 986 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2187 2466 3814 4715 4977 820
Size: 197 926 244 279 1348 901 262 1553
Fragments arranged by size:
1553 1348 926 901 279 262 244 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 2446 3068 1132
Size: 1314 622 3774
Fragments arranged by size:
3774 1314 622
SspI AAT'ATT
Cuts at: 5 52 2524 3634 5
Size: 47 2472 1110 2081
Fragments arranged by size:
2472 2081 1110 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2126 3149 513
Size: 552 939 122 1023 3074
Fragments arranged by size:
3074 1023 939 552 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2313 3737 3754 3907 4092 4537 325
Size: 1255 733 1424 17 153 185 445 1498
Fragments arranged by size:
1498 1424 1255 733 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2822 3956 5668
Size: 274 80 33 51 1443 845 1134 1712
Cuts at: 5668 96
Size: 138
Fragments arranged by size:
1712 1443 1134 845 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 2220 2508 2562 2868 3839 2220
Size: 288 54 306 971 4091
Fragments arranged by size:
4091 971 306 288 54
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI