Restriction map for
pFN21AB6413
(Circular) (Six-base) MAPSORT of: pFN21AB6413.seq Check: 3142 from: 1 to: 5629
pFN21AB6413 5629 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2512 2512
Size: 5629
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2580 3439 278
Size: 53 83 186 936 1044 859 2468
Fragments arranged by size:
2468 1044 936 859 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2874 2972 1937
Size: 937 98 4594
Fragments arranged by size:
4594 937 98
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 2905 3692 5076 5344 1632
Size: 662 611 787 1384 268 1917
Fragments arranged by size:
1917 1384 787 662 611 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 2334 4528 5505 1203
Size: 1131 2194 977 1327
Fragments arranged by size:
2194 1327 1131 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 2818 3372 4944 2818
Size: 554 1572 3503
Fragments arranged by size:
3503 1572 554
ApaI G_GGCC'C
Cuts at: 2590 2590
Size: 5629
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2938 3164 3316 5425 1493
Size: 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2426 2954 1870
Size: 73 483 528 4545
Fragments arranged by size:
4545 528 483 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2710 2950 4405 618
Size: 325 217 627 298 625 240 1455 1842
Fragments arranged by size:
1842 1455 627 625 325 298 240 217
BanII G_rGCy'C
Cuts at: 1391 2048 2590 2948 1391
Size: 657 542 358 4072
Fragments arranged by size:
4072 657 542 358
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 1903
Size: 34 1883 34 3678
Fragments arranged by size:
3678 1883 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3521 4731 3521
Size: 1210 4419
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BfrBI ATG'CAT
Cuts at: 2848 2848
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 2590 3690 4846 1790
Size: 800 1100 1156 2573
Fragments arranged by size:
2573 1156 1100 800
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2640 2788 2851
Size: 416 53 83 399 24 1223 148 63
Cuts at: 2851 2887 3360 4318 442
Size: 36 473 958 1753
Fragments arranged by size:
1753 1223 958 473 416 399 148 83
63 53 36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 4289 1239
Size: 914 2136 2579
Fragments arranged by size:
2579 2136 914
Bpu10I CC'TnA_GC
Cuts at: 2238 2661 2238
Size: 423 5206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 2138 2749 3754 4619 4917 2000
Size: 138 611 1005 865 298 2712
Fragments arranged by size:
2712 1005 865 611 298 138
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 882
Size: 617 2793 2219
Fragments arranged by size:
2793 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2577 3436 3818 4453
Size: 53 83 186 936 1044 859 382 635
Cuts at: 4453 275
Size: 1451
Fragments arranged by size:
1451 1044 936 859 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2040 4062 4460 4734 4881 5292 2040
Size: 2022 398 274 147 411 2377
Fragments arranged by size:
2377 2022 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2336 2366 2562 2592 1178
Size: 30 165 30 933 30 196 30 4215
Fragments arranged by size:
4215 933 196 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2666 1186
Size: 1024 456 4149
Fragments arranged by size:
4149 1024 456
BseYI C'CCAG_C
Cuts at: 2048 2067 4832 5328 2048
Size: 19 2765 496 2349
Fragments arranged by size:
2765 2349 496 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2431 2584 1163
Size: 573 695 153 4208
Fragments arranged by size:
4208 695 573 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2208 3079 3840 4868 1540
Size: 275 186 207 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 207 186
BsiHKAI G_wGCw'C
Cuts at: 3690 3775 4846 3690
Size: 85 1071 4473
Fragments arranged by size:
4473 1071 85
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
BsmBI CGTCTCn'nnnn_
Cuts at: 2117 2117
Size: 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2590 2948 3690 3775 4846 1391
Size: 399 258 542 358 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 542 399 358 258 85
BspEI T'CCGG_A
Cuts at: 2040 5292 2040
Size: 3252 2377
BspHI T'CATG_A
Cuts at: 3518 5248 3518
Size: 1730 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2512 2646 2984 844
Size: 1668 134 338 3489
Fragments arranged by size:
3489 1668 338 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4298 65
Size: 1183 2876 174 1396
Fragments arranged by size:
2876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 4273 4460 1700
Size: 110 272 2191 187 2869
Fragments arranged by size:
2869 2191 272 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 3689 4701 3689
Size: 1012 4617
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2701 2701
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2349 2959 3326 3711 3728 5180 5266 5278
Size: 377 610 367 385 17 1452 86 12
Cuts at: 5278 5624 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 610 385 377 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2811 2811
Size: 5629
BtgI C'CryG_G
Cuts at: 513 1065 2004 2285 2676 2714 513
Size: 552 939 281 391 38 3428
Fragments arranged by size:
3428 939 552 391 281 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2383 487
Size: 683 56 1157 3733
Fragments arranged by size:
3733 1157 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3145 3939 3959 2746
Size: 399 794 20 4416
Fragments arranged by size:
4416 794 399 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2709 2744 529
Size: 35 2145 35 3414
Fragments arranged by size:
3414 2145 35 35
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2080 2205
Size: 54 1425 50 275 18 225 25 125
Cuts at: 2205 2717 3076 3965 5594 8
Size: 512 359 889 1629 43
Fragments arranged by size:
1629 1425 889 512 359 275 225 125
54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2205 3076 1537
Size: 275 393 871 4090
Fragments arranged by size:
4090 871 393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 2540 2609 2924 3559 1353
Size: 1176 11 69 315 635 3423
Fragments arranged by size:
3423 1176 635 315 69 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2294 2905 3692 4289 5076 5344
Size: 393 521 141 611 787 597 787 268
Cuts at: 5344 1239
Size: 1524
Fragments arranged by size:
1524 787 787 611 597 521 393 268
141
EcoO109I rG'GnC_Cy
Cuts at: 2586 2665 2815 2586
Size: 79 150 5400
Fragments arranged by size:
5400 150 79
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 2036 2688 4776 1678
Size: 358 652 2088 2531
Fragments arranged by size:
2531 2088 652 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2321 2353 2562
Size: 32 135 32 614 32 103 32 209
Cuts at: 2562 2594 2794 2826 4270 4302 4344 4376 1373
Size: 32 200 32 1444 32 42 32 2626
Fragments arranged by size:
2626 1444 614 209 200 135 103 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2875 2973 3219 669
Size: 988 281 937 98 246 3079
Fragments arranged by size:
3079 988 937 281 246 98
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4743 4927 1305
Size: 3438 184 2007
Fragments arranged by size:
3438 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 3582
Fragments arranged by size:
3582 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 3587 3946 4105 518
Size: 648 1533 888 359 159 2042
Fragments arranged by size:
2042 1533 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2671 2716 2898 3045 3722 4870 5115 1606
Size: 1065 45 182 147 677 1148 245 2120
Fragments arranged by size:
2120 1148 1065 677 245 182 147 45
NaeI GCC'GGC
Cuts at: 2084 2084
Size: 5629
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4138
Fragments arranged by size:
4138 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NgoMIV G'CCGG_C
Cuts at: 2082 2082
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NsiI A_TGCA'T
Cuts at: 2850 2850
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2848 2987 4532 1132
Size: 97 1619 139 1545 2229
Fragments arranged by size:
2229 1619 1545 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5629
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5629
PfoI T'CCnGG_A
Cuts at: 2817 2817
Size: 5629
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2815 2815
Size: 5629
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5629
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PspOMI G'GGCC_C
Cuts at: 2586 2586
Size: 5629
PspXI vC'TCGA_Gb
Cuts at: 2426 2426
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2262 2981 830
Size: 1432 719 3478
Fragments arranged by size:
3478 1432 719
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
SacII CC_GC'GG
Cuts at: 2717 2717
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2873 2971 1936
Size: 937 98 4594
Fragments arranged by size:
4594 937 98
SapI GCTCTTCn'nnn_
Cuts at: 2609 2609
Size: 5629
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2232 2258 2977 4112 4793 4984 826
Size: 219 1187 26 719 1135 681 191 1471
Fragments arranged by size:
1471 1187 1135 719 681 219 191 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 2153 2426 2728 3733 4634
Size: 197 926 72 138 273 302 1005 901
Cuts at: 4634 4896 820
Size: 262 1553
Fragments arranged by size:
1553 1005 926 901 302 273 262 197
138 72
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 2495 1517
Size: 978 4651
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 2529 3068 513
Size: 552 939 183 342 539 3074
Fragments arranged by size:
3074 939 552 539 342 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3656 3673 3826 4011 4456 325
Size: 1255 2076 17 153 185 445 1498
Fragments arranged by size:
2076 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5587 96
Size: 274 80 33 51 1443 1898 1712 138
Fragments arranged by size:
1898 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2458 2458
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 2426 1943
Size: 483 5146
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 3758 3758
Size: 5629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2578 3437 276
Size: 53 83 186 936 1044 859 2468
Fragments arranged by size:
2468 1044 936 859 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI PacI PmlI PsrI PvuII
RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI