Restriction map for
pFN21AB6412
(Circular) (Six-base) MAPSORT of: pFN21AB6412.seq Check: 317 from: 1 to: 5710
pFN21AB6412 5710 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2057 2057
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2445 2712 2821 3773 5157 5425 1632
Size: 813 267 109 952 1384 268 1917
Fragments arranged by size:
1917 1384 952 813 268 267 109
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 2153 2502 2826 1166
Size: 987 349 324 4050
Fragments arranged by size:
4050 987 349 324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 2407 2437 3453 5025 2407
Size: 30 1016 1572 3092
Fragments arranged by size:
3092 1572 1016 30
ApaI G_GGCC'C
Cuts at: 2609 2609
Size: 5710
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2530 3035 1870
Size: 73 587 505 4545
Fragments arranged by size:
4545 587 505 73
BamHI G'GATC_C
Cuts at: 2457 3040 3407 2457
Size: 583 367 4760
Fragments arranged by size:
4760 583 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2397 3031 4486 618
Size: 325 217 627 610 634 1455 1842
Fragments arranged by size:
1842 1455 634 627 610 325 217
BanII G_rGCy'C
Cuts at: 1391 2537 2609 3029 1391
Size: 1146 72 420 4072
Fragments arranged by size:
4072 1146 420 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2225 928
Size: 971 326 4413
Fragments arranged by size:
4413 971 326
BbvCI CC'TCA_GC
Cuts at: 2928 2928
Size: 5710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BfrBI ATG'CAT
Cuts at: 2355 2355
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2609 3771 4927 1790
Size: 819 1162 1156 2573
Fragments arranged by size:
2573 1162 1156 819
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3014
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2636 4370 1239
Size: 965 432 1734 2579
Fragments arranged by size:
2579 1734 965 432
Bpu10I CC'TnA_GC
Cuts at: 2234 2928 2234
Size: 694 5016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2479 2832 3517 3899
Size: 53 83 186 936 946 353 685 382
Cuts at: 3899 4534 275
Size: 635 1451
Fragments arranged by size:
1451 946 936 685 635 382 353 186
83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2677 2916 2990 1186
Size: 1491 239 74 3906
Fragments arranged by size:
3906 1491 239 74
BseYI C'CCAG_C
Cuts at: 2056 4913 5409 2056
Size: 2857 496 2357
Fragments arranged by size:
2857 2357 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2154 3160 3921 4949 1540
Size: 275 186 153 1006 761 1028 2301
Fragments arranged by size:
2301 1028 1006 761 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 2357 3221 3314 2357
Size: 864 93 4753
Fragments arranged by size:
4753 864 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2537 2609 3029 3771 3856 4927 1391
Size: 399 747 72 420 742 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 420 399 85 72
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 3065 844
Size: 1213 1008 3489
Fragments arranged by size:
3489 1213 1008
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2394 2747 4354 4541 1700
Size: 110 584 353 1607 187 2869
Fragments arranged by size:
2869 1607 584 353 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 2009 2446 1184
Size: 825 437 4448
Fragments arranged by size:
4448 825 437
BstXI CCAn_nnnn'nTGG
Cuts at: 2064 2504 2064
Size: 440 5270
BstYI r'GATC_y
Cuts at: 1972 2457 3040 3407 3792 3809 5261 5347 5359
Size: 485 583 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 583 485 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2503 2677 513
Size: 552 939 499 174 3546
Fragments arranged by size:
3546 939 552 499 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 2090 2417 2920 3226 4020 4040 2090
Size: 327 503 306 794 20 3760
Fragments arranged by size:
3760 794 503 327 306 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2900 4717 809
Size: 507 1584 1817 1802
Fragments arranged by size:
1817 1802 1584 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2314 2641 3157
Size: 54 1425 50 275 18 484 327 516
Cuts at: 3157 4046 5675 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 516 484 327 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2172 2688 3640 1353
Size: 756 63 516 952 3423
Fragments arranged by size:
3423 952 756 516 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2445 2636 2712 2821 3773 4370
Size: 393 572 241 191 76 109 952 597
Cuts at: 4370 5157 5425 1239
Size: 787 268 1524
Fragments arranged by size:
1524 952 787 597 572 393 268 241
191 109 76
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2270 2129
Size: 141 5569
EcoO109I rG'GnC_Cy
Cuts at: 2068 2134 2229 2605 2720 2068
Size: 66 95 376 115 5058
Fragments arranged by size:
5058 376 115 95 66
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 4857 1678
Size: 3179 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2121 2153 4351 4383 4425
Size: 32 135 32 549 32 2198 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 2198 549 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2502 2826 2853 3668 4027 4186
Size: 648 987 349 324 27 815 359 159
Cuts at: 4186 518
Size: 2042
Fragments arranged by size:
2042 987 815 648 359 349 324 159
27
MspA1I CmG'CkG
Cuts at: 1606 2020 2029 2658 3126 3803 4951 5196 1606
Size: 414 9 629 468 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 629 468 414 245 9
NaeI GCC'GGC
Cuts at: 2396 2396
Size: 5710
NcoI C'CATG_G
Cuts at: 513 1065 2004 2677 513
Size: 552 939 673 3546
Fragments arranged by size:
3546 939 673 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NgoMIV G'CCGG_C
Cuts at: 2394 2394
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NsiI A_TGCA'T
Cuts at: 2357 2357
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2083 2819 3068 4613 1132
Size: 97 854 736 249 1545 2229
Fragments arranged by size:
2229 1545 854 736 249 97
PasI CC'CwG_GG
Cuts at: 1348 2125 1348
Size: 777 4933
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2985 1108
Size: 1877 3833
PfoI T'CCnGG_A
Cuts at: 2460 2613 2460
Size: 153 5557
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2229 2134
Size: 95 5615
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspOMI G'GGCC_C
Cuts at: 2605 2605
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2144 2412 3062 830
Size: 1314 268 650 3478
Fragments arranged by size:
3478 1314 650 268
PvuI CG_AT'CG
Cuts at: 2001 2154 2001
Size: 153 5557
PvuII CAG'CTG
Cuts at: 2658 2658
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SapI GCTCTTCn'nnn_
Cuts at: 2172 2172
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2140 2408 3058 4193 4874 5065 826
Size: 219 1095 268 650 1135 681 191 1471
Fragments arranged by size:
1471 1135 1095 681 650 268 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 2532 3037 1872
Size: 660 505 4545
Fragments arranged by size:
4545 660 505
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 2083 3068 1132
Size: 951 985 3774
Fragments arranged by size:
3774 985 951
SrfI GCCC'GGGC
Cuts at: 2532 2532
Size: 5710
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2677 3149 513
Size: 552 939 673 472 3074
Fragments arranged by size:
3074 939 673 552 472
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2690 2816 2904 3737 3754 3907 4092
Size: 1255 1110 126 88 833 17 153 185
Cuts at: 4092 4537 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1110 833 445 185 153 126
88 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2867 3956 5668
Size: 274 80 33 51 1443 890 1089 1712
Cuts at: 5668 96
Size: 138
Fragments arranged by size:
1712 1443 1089 890 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2912 2309
Size: 603 5107
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 2530 3035 1870
Size: 660 505 4545
Fragments arranged by size:
4545 660 505
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SwaI