Restriction map for
pFN21AB6411
(Circular) (Six-base) MAPSORT of: pFN21AB6411.seq Check: 9389 from: 1 to: 5626
pFN21AB6411 5626 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2220 3436 278
Size: 53 83 186 936 684 1216 2468
Fragments arranged by size:
2468 1216 936 684 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 2282 3753 4126 1563
Size: 719 1471 373 3063
Fragments arranged by size:
3063 1471 719 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3689 5073 5341 1632
Size: 2057 1384 268 1917
Fragments arranged by size:
2057 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2303 820
Size: 197 1286 4143
Fragments arranged by size:
4143 1286 197
AflIII A'CryG_T
Cuts at: 1203 4525 5502 1203
Size: 3322 977 1327
Fragments arranged by size:
3322 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 2524 3369 4941 2524
Size: 845 1572 3209
Fragments arranged by size:
3209 1572 845
ApaI G_GGCC'C
Cuts at: 2577 2577
Size: 5626
ApaLI G'TGCA_C
Cuts at: 2492 3683 4839 2492
Size: 1191 1156 3279
Fragments arranged by size:
3279 1191 1156
ApoI r'AATT_y
Cuts at: 1493 2935 3161 3313 5422 1493
Size: 1442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2634 2951 1870
Size: 73 691 317 4545
Fragments arranged by size:
4545 691 317 73
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2560 2779 2947 4402 618
Size: 325 217 627 773 219 168 1455 1842
Fragments arranged by size:
1842 1455 773 627 325 219 217 168
BanII G_rGCy'C
Cuts at: 1391 2061 2577 2633 2945 1391
Size: 670 516 56 312 4072
Fragments arranged by size:
4072 670 516 312 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2613 928
Size: 971 714 3941
Fragments arranged by size:
3941 971 714
BbvCI CC'TCA_GC
Cuts at: 2453 2453
Size: 5626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2030 2064 3817 3851 1903
Size: 34 93 34 1753 34 3678
Fragments arranged by size:
3678 1753 93 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2106 2439 2481 5621 2106
Size: 333 42 3140 2111
Fragments arranged by size:
3140 2111 333 42
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2496 2577 2716 3687 4843 1790
Size: 706 81 139 971 1156 2573
Fragments arranged by size:
2573 1156 971 706 139 81
BmgBI CAC'GTC
Cuts at: 1318 2665 1318
Size: 1347 4279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2668 3357 4315
Size: 416 53 83 399 24 1251 689 958
Cuts at: 4315 442
Size: 1753
Fragments arranged by size:
1753 1251 958 689 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2825 4286 1239
Size: 1586 1461 2579
Fragments arranged by size:
2579 1586 1461
Bpu10I CC'TnA_GC
Cuts at: 2094 2453 2094
Size: 359 5267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 4289 882
Size: 617 720 2070 2219
Fragments arranged by size:
2219 2070 720 617
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 3433 3815 4450
Size: 53 83 186 936 684 1216 382 635
Cuts at: 4450 275
Size: 1451
Fragments arranged by size:
1451 1216 936 684 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5401
Fragments arranged by size:
5401 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2579 2652 1186
Size: 946 447 73 4160
Fragments arranged by size:
4160 946 447 73
BseYI C'CCAG_C
Cuts at: 2619 4829 5325 2619
Size: 2210 496 2920
Fragments arranged by size:
2920 2210 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2416 1163
Size: 573 414 266 4373
Fragments arranged by size:
4373 573 414 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2548 3076 3837 4865 1540
Size: 275 186 547 528 761 1028 2301
Fragments arranged by size:
2301 1028 761 547 528 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2496 2766 3687 3772 4843 2061
Size: 435 270 921 85 1071 2844
Fragments arranged by size:
2844 1071 921 435 270 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
BsmBI CGTCTCn'nnnn_
Cuts at: 2750 2750
Size: 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2496 2577 2633 2716 2766 2945
Size: 399 271 435 81 56 83 50 179
Cuts at: 2945 3687 3772 4843 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 271 179 85
83 81 56 50
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 2566 3515 5245 2566
Size: 949 1730 2947
Fragments arranged by size:
2947 1730 949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2359 2981 844
Size: 1515 622 3489
Fragments arranged by size:
3489 1515 622
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4121 4295 65
Size: 1183 2873 174 1396
Fragments arranged by size:
2873 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 2642 4270 4457 1700
Size: 110 218 614 1628 187 2869
Fragments arranged by size:
2869 1628 614 218 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2560 794
Size: 1766 3860
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2106 2439 2481 2956 3323 3708 3725 5177
Size: 134 333 42 475 367 385 17 1452
Cuts at: 5177 5263 5275 5621 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 475 385 367 346 333 134
86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2170 2260 2907 513
Size: 552 939 166 90 647 3232
Fragments arranged by size:
3232 939 647 552 166 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 3142 3936 3956 2665
Size: 477 794 20 4335
Fragments arranged by size:
4335 794 477 20
ClaI AT'CG_AT
Cuts at: 1554 2472 1554
Size: 918 4708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DraIII CAC_nnn'GTG
Cuts at: 2157 2772 2157
Size: 615 5011
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2147 4633 809
Size: 507 831 2486 1802
Fragments arranged by size:
2486 1802 831 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2910 3073 3962
Size: 54 1425 50 275 18 1080 163 889
Cuts at: 3962 5591 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1080 889 275 163 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 3556 1353
Size: 890 1313 3423
Fragments arranged by size:
3423 1313 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4213 4587 4733 387
Size: 1064 138 435 2189 374 146 1280
Fragments arranged by size:
2189 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2059 2059
Size: 5626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2825 3689 4286 5073 5341 1239
Size: 393 1193 864 597 787 268 1524
Fragments arranged by size:
1524 1193 864 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2083 2500 2083
Size: 417 5209
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 5626
FspI TGC'GCA
Cuts at: 2399 4132 2399
Size: 1733 3893
HaeII r_GCGC'y
Cuts at: 1678 2428 4773 1678
Size: 750 2345 2531
Fragments arranged by size:
2531 2345 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2512 2544 4267 4299 4341
Size: 32 135 32 940 32 1723 32 42
Cuts at: 4341 4373 1373
Size: 32 2626
Fragments arranged by size:
2626 1723 940 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4740 4924 1305
Size: 3435 184 2007
Fragments arranged by size:
3435 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3804
Fragments arranged by size:
3804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 2751 3584 3943 4102 518
Size: 648 1533 52 833 359 159 2042
Fragments arranged by size:
2042 1533 833 648 359 159 52
MspA1I CmG'CkG
Cuts at: 1606 2044 2799 3042 3719 4867 5112 1606
Size: 438 755 243 677 1148 245 2120
Fragments arranged by size:
2120 1148 755 677 438 245 243
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 5626
NcoI C'CATG_G
Cuts at: 513 1065 2004 2170 513
Size: 552 939 166 3969
Fragments arranged by size:
3969 939 552 166
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2984 4529 1132
Size: 97 1755 1545 2229
Fragments arranged by size:
2229 1755 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2524 2619 2722 1108
Size: 1416 95 103 4012
Fragments arranged by size:
4012 1416 103 95
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PspOMI G'GGCC_C
Cuts at: 2573 2573
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2978 830
Size: 2148 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
PvuII CAG'CTG
Cuts at: 2044 2044
Size: 5626
SacI G_AGCT'C
Cuts at: 2061 2061
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2269 2974 4109 4790 4981 826
Size: 219 1224 705 1135 681 191 1471
Fragments arranged by size:
1471 1224 1135 705 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 3730 4631 4893 820
Size: 197 926 360 1427 901 262 1553
Fragments arranged by size:
1553 1427 926 901 360 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 2004 2170 3065 513
Size: 552 939 166 895 3074
Fragments arranged by size:
3074 939 895 552 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2562 2797 3653 3670 3823 4008 4453
Size: 1255 982 235 856 17 153 185 445
Cuts at: 4453 325
Size: 1498
Fragments arranged by size:
1498 1255 982 856 445 235 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3872 5584 96
Size: 274 80 33 51 1443 1895 1712 138
Fragments arranged by size:
1895 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2874 2906 5586 5618 2874
Size: 32 2680 32 2882
Fragments arranged by size:
2882 2680 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2521 2824 2905 2107
Size: 414 303 81 4828
Fragments arranged by size:
4828 414 303 81
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 2849 3755 2849
Size: 906 4720
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2218 3434 276
Size: 53 83 186 936 684 1216 2468
Fragments arranged by size:
2468 1216 936 684 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI