Restriction map for
pFN21AB6410
(Circular) (Six-base) MAPSORT of: pFN21AB6410.seq Check: 9923 from: 1 to: 5710
pFN21AB6410 5710 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2404 2404
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2610 3520 278
Size: 53 83 186 936 1074 910 2468
Fragments arranged by size:
2468 1074 936 910 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 3773 5157 5425 1632
Size: 896 1245 1384 268 1917
Fragments arranged by size:
1917 1384 1245 896 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2436 2848 1184
Size: 1252 412 4046
Fragments arranged by size:
4046 1252 412
AlwNI CAG_nnn'CTG
Cuts at: 2195 3453 5025 2195
Size: 1258 1572 2880
Fragments arranged by size:
2880 1572 1258
ApaI G_GGCC'C
Cuts at: 2243 2243
Size: 5710
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2499 3035 1870
Size: 73 556 536 4545
Fragments arranged by size:
4545 556 536 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2957 2990 2957
Size: 33 5677
BamHI G'GATC_C
Cuts at: 2622 3040 3407 2622
Size: 418 367 4925
Fragments arranged by size:
4925 418 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 3031 4486 618
Size: 325 217 627 465 779 1455 1842
Fragments arranged by size:
1842 1455 779 627 465 325 217
BanII G_rGCy'C
Cuts at: 1391 2243 2851 3029 1391
Size: 852 608 178 4072
Fragments arranged by size:
4072 852 608 178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2207 2241 3901 3935 1903
Size: 34 50 34 186 34 1660 34 3678
Fragments arranged by size:
3678 1660 186 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2528 3602 4812 2528
Size: 1074 1210 3426
Fragments arranged by size:
3426 1210 1074
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4321 136
Size: 107 122 71 631 605 337 2312 1525
Fragments arranged by size:
2312 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2243 2257 3771 4927 1790
Size: 453 14 1514 1156 2573
Fragments arranged by size:
2573 1514 1156 453 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 3022 4370 1239
Size: 1149 634 1348 2579
Fragments arranged by size:
2579 1348 1149 634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2607 3517 3899 4534
Size: 53 83 186 936 1074 910 382 635
Cuts at: 4534 275
Size: 1451
Fragments arranged by size:
1451 1074 936 910 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2802 1186
Size: 1616 4094
BseYI C'CCAG_C
Cuts at: 2017 2295 2913 4913 5409 2017
Size: 278 618 2000 496 2318
Fragments arranged by size:
2318 2000 618 496 278
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
BsmBI CGTCTCn'nnnn_
Cuts at: 2614 2614
Size: 5710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2257 2851 3029 3771 3856 4927
Size: 399 453 14 594 178 742 85 1071
Cuts at: 4927 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 594 453 399 178 85
14
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2404 3065 844
Size: 1334 226 661 3489
Fragments arranged by size:
3489 1334 661 226
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2223 2727 4205 4379 65
Size: 1183 975 504 1478 174 1396
Fragments arranged by size:
1478 1396 1183 975 504 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 4354 4541 1700
Size: 110 198 2346 187 2869
Fragments arranged by size:
2869 2346 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 2995 1184
Size: 1811 3899
BstXI CCAn_nnnn'nTGG
Cuts at: 2843 2843
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2622 2686 3040 3407 3792 3809 5261 5347
Size: 650 64 354 367 385 17 1452 86
Cuts at: 5347 5359 5705 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 650 385 367 354 346 86
64 17 12
Bsu36I CC'TnA_GG
Cuts at: 2682 2682
Size: 5710
BtgI C'CryG_G
Cuts at: 513 1065 2087 2748 513
Size: 552 1022 661 3475
Fragments arranged by size:
3475 1022 661 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2909 487
Size: 683 56 1683 3288
Fragments arranged by size:
3288 1683 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 3226 4020 4040 2478
Size: 748 794 20 4148
Fragments arranged by size:
4148 794 748 20
ClaI AT'CG_AT
Cuts at: 1554 2463 1554
Size: 909 4801
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DraIII CAC_nnn'GTG
Cuts at: 2759 2951 2759
Size: 192 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2661 3157 4046
Size: 54 1425 50 275 18 831 496 889
Cuts at: 4046 5675 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 831 496 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3640 1353
Size: 2287 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2107 4297 4671 4817 387
Size: 1064 138 518 2190 374 146 1280
Fragments arranged by size:
2190 1280 1064 518 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2528 3022 3773 4370 5157 5425
Size: 393 756 140 494 751 597 787 268
Cuts at: 5425 1239
Size: 1524
Fragments arranged by size:
1524 787 756 751 597 494 393 268
140
EcoNI CCTnn'n_nnAGG
Cuts at: 2115 2174 2115
Size: 59 5651
EcoO109I rG'GnC_Cy
Cuts at: 2239 2240 2773 2940 2239
Size: 1 533 167 5009
Fragments arranged by size:
5009 533 167 1
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
EcoRV GAT'ATC
Cuts at: 2215 2215
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2037 4857 1678
Size: 359 2820 2531
Fragments arranged by size:
2820 2531 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2145 2177 4351 4383 4425
Size: 32 135 32 573 32 2174 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 2174 573 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2663 10
Size: 54 1425 343 831 3057
Fragments arranged by size:
3057 1425 831 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 3668 4027 4186 518
Size: 648 1182 1320 359 159 2042
Fragments arranged by size:
2042 1320 1182 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2299 3126 3803 4951 5196 1606
Size: 483 210 827 677 1148 245 2120
Fragments arranged by size:
2120 1148 827 677 483 245 210
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 5710
NcoI C'CATG_G
Cuts at: 513 1065 2748 513
Size: 552 1683 3475
Fragments arranged by size:
3475 1683 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PfoI T'CCnGG_A
Cuts at: 2530 2530
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2940 2940
Size: 5710
PshAI GACnn'nnGTC
Cuts at: 1613 2099 2526 1613
Size: 486 427 4797
Fragments arranged by size:
4797 486 427
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspOMI G'GGCC_C
Cuts at: 2239 2239
Size: 5710
PspXI vC'TCGA_Gb
Cuts at: 2499 2499
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2178 2706 3062 830
Size: 1348 528 356 3478
Fragments arranged by size:
3478 1348 528 356
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2299 2299
Size: 5710
SacII CC_GC'GG
Cuts at: 2090 2090
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 2706 3062 2706
Size: 356 5354
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2174 2702 2810 2862 3058 4193 4874
Size: 219 1129 528 108 52 196 1135 681
Cuts at: 4874 5065 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1129 681 528 219 196 191
108 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2499 3814 4715 4977 820
Size: 197 926 556 1315 901 262 1553
Fragments arranged by size:
1553 1315 926 901 556 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2235 2748 3149 513
Size: 552 1170 513 401 3074
Fragments arranged by size:
3074 1170 552 513 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2240 2309 3737 3754 3907 4092 4537
Size: 1255 660 69 1428 17 153 185 445
Cuts at: 4537 325
Size: 1498
Fragments arranged by size:
1498 1428 1255 660 445 185 153 69
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2675 3956 5668
Size: 274 80 33 51 1443 698 1281 1712
Cuts at: 5668 96
Size: 138
Fragments arranged by size:
1712 1443 1281 698 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 2499 1943
Size: 556 5154
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2608 3518 276
Size: 53 83 186 936 1074 910 2468
Fragments arranged by size:
2468 1074 936 910 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I EcoICRI FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI