Restriction map for
pFN21AB6408
(Circular) (Six-base) MAPSORT of: pFN21AB6408.seq Check: 7391 from: 1 to: 5710
pFN21AB6408 5710 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 2355 3031 2355
Size: 676 5034
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2653 2858 3773 5157 5425 1632
Size: 1021 205 915 1384 268 1917
Fragments arranged by size:
1917 1384 1021 915 268 205
AfeI AGC'GCT
Cuts at: 2393 2393
Size: 5710
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2150 1184
Size: 966 4744
AlwNI CAG_nnn'CTG
Cuts at: 2942 2954 3453 5025 2942
Size: 12 499 1572 3627
Fragments arranged by size:
3627 1572 499 12
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2968 3019 3245 3397 5506 1493
Size: 1475 51 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152 51
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BamHI G'GATC_C
Cuts at: 2008 3040 3407 2008
Size: 1032 367 4311
Fragments arranged by size:
4311 1032 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2355 2546 3031 4486 618
Size: 325 217 627 568 191 485 1455 1842
Fragments arranged by size:
1842 1455 627 568 485 325 217 191
BanII G_rGCy'C
Cuts at: 1391 2022 3029 1391
Size: 631 1007 4072
Fragments arranged by size:
4072 1007 631
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2316 3602 4812 2316
Size: 1286 1210 3214
Fragments arranged by size:
3214 1286 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2549 3771 4927 1790
Size: 759 1222 1156 2573
Fragments arranged by size:
2573 1222 1156 759
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2498 3441 4399
Size: 416 53 83 399 24 1081 943 958
Cuts at: 4399 442
Size: 1753
Fragments arranged by size:
1753 1081 958 943 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2324 2390 2418 2551 4370 1239
Size: 995 90 66 28 133 1819 2579
Fragments arranged by size:
2579 1819 995 133 90 66 28
Bpu10I CC'TnA_GC
Cuts at: 2901 2901
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2431 4373 882
Size: 617 932 1942 2219
Fragments arranged by size:
2219 1942 932 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 2759 5704 2759
Size: 2945 2765
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 1186
Size: 826 4884
BseYI C'CCAG_C
Cuts at: 2563 2604 2729 2896 4913 5409 2563
Size: 41 125 167 2017 496 2864
Fragments arranged by size:
2864 2017 496 167 125 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2461 2479 2614 1163
Size: 573 725 18 135 4259
Fragments arranged by size:
4259 725 573 135 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2549 3029 3771 3856 4927 1391
Size: 399 232 527 480 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 527 480 399 232 85
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 2751 3599 5329 2751
Size: 848 1730 3132
Fragments arranged by size:
3132 1730 848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2413 2483 3065 844
Size: 1569 70 582 3489
Fragments arranged by size:
3489 1569 582 70
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2726 794
Size: 1932 3778
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2008 3040 3407 3792 3809 5261 5347 5359
Size: 36 1032 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1032 385 367 346 86 36
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2675 2714 513
Size: 552 939 671 39 3509
Fragments arranged by size:
3509 939 671 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 2727 3226 4020 4040 2727
Size: 499 794 20 4397
Fragments arranged by size:
4397 794 499 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 2451 3014 2451
Size: 563 5147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675
Size: 54 1425 50 275 18 1327 889 1629
Cuts at: 5675 8
Size: 43
Fragments arranged by size:
1629 1425 1327 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2785 2877 3640 1353
Size: 1432 92 763 3423
Fragments arranged by size:
3423 1432 763 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2324 2390 2418 2551 2653 2858
Size: 393 602 90 66 28 133 102 205
Cuts at: 2858 3773 4370 5157 5425 1239
Size: 915 597 787 268 1524
Fragments arranged by size:
1524 915 787 602 597 393 268 205
133 102 90 66 28
EcoO109I rG'GnC_Cy
Cuts at: 2264 2472 2867 2905 2264
Size: 208 395 38 5069
Fragments arranged by size:
5069 395 208 38
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2395 4857 1678
Size: 717 2462 2531
Fragments arranged by size:
2531 2462 717
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4351 4383 4425 4457 1373
Size: 32 135 32 2779 32 42 32 2626
Fragments arranged by size:
2779 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 2359 3035 2359
Size: 676 5034
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2765 3668 4027 4186 518
Size: 648 1599 903 359 159 2042
Fragments arranged by size:
2042 1599 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2529 3126 3803 4951 5196 1606
Size: 923 597 677 1148 245 2120
Fragments arranged by size:
2120 1148 923 677 597 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2714 513
Size: 552 939 710 3509
Fragments arranged by size:
3509 939 710 552
NdeI CA'TA_TG
Cuts at: 387 2149 387
Size: 1762 3948
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2612 3068 4613 1132
Size: 97 1383 456 1545 2229
Fragments arranged by size:
2229 1545 1383 456 97
PasI CC'CwG_GG
Cuts at: 1348 2317 1348
Size: 969 4741
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PfoI T'CCnGG_A
Cuts at: 2958 2958
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2472 2867 2905 2472
Size: 395 38 5277
Fragments arranged by size:
5277 395 38
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2406 3062 830
Size: 1576 656 3478
Fragments arranged by size:
3478 1576 656
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2529 2529
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SexAI A'CCwGG_T
Cuts at: 2863 2863
Size: 5710
SfcI C'TryA_G
Cuts at: 826 1045 2402 2777 3058 4193 4874 5065 826
Size: 219 1357 375 281 1135 681 191 1471
Fragments arranged by size:
1471 1357 1135 681 375 281 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 2612 3068 1132
Size: 1480 456 3774
Fragments arranged by size:
3774 1480 456
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 2714 3149 513
Size: 552 939 9 701 435 3074
Fragments arranged by size:
3074 939 701 552 435 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 2568 3737 3754 3907 4092 4537
Size: 1255 984 4 1169 17 153 185 445
Cuts at: 4537 325
Size: 1498
Fragments arranged by size:
1498 1255 1169 984 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2115 2147 2570 2602 5670 5702 2115
Size: 32 423 32 3068 32 2123
Fragments arranged by size:
3068 2123 423 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI