Restriction map for
pFN21AB6405
(Circular) (Six-base) MAPSORT of: pFN21AB6405.seq Check: 9489 from: 1 to: 5620
pFN21AB6405 5620 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2742 3430 278
Size: 53 83 186 936 1206 688 2468
Fragments arranged by size:
2468 1206 936 688 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 2823 2963 1937
Size: 517 369 140 4594
Fragments arranged by size:
4594 517 369 140
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2616 3683 5067 5335 1632
Size: 984 1067 1384 268 1917
Fragments arranged by size:
1917 1384 1067 984 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2534 1166
Size: 1368 4252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2362 1184
Size: 1178 4442
AlwNI CAG_nnn'CTG
Cuts at: 2078 2137 3363 4935 2078
Size: 59 1226 1572 2763
Fragments arranged by size:
2763 1572 1226 59
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2929 3155 3307 5416 1493
Size: 1436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 2904 2939 1391
Size: 826 687 35 4072
Fragments arranged by size:
4072 826 687 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BbvCI CC'TCA_GC
Cuts at: 2068 2884 2068
Size: 816 4804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2813 2847 3811 3845 1903
Size: 34 876 34 964 34 3678
Fragments arranged by size:
3678 964 876 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2432 4231 136
Size: 107 122 71 631 605 760 1799 1525
Fragments arranged by size:
1799 1525 760 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2341 2575 5615 2341
Size: 234 3040 2346
Fragments arranged by size:
3040 2346 234
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2380 4280 1239
Size: 878 263 1900 2579
Fragments arranged by size:
2579 1900 878 263
Bpu10I CC'TnA_GC
Cuts at: 2068 2884 2068
Size: 816 4804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2759 3745 4610 4908 2759
Size: 986 865 298 3471
Fragments arranged by size:
3471 986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2739 3427 3809 4444
Size: 53 83 186 936 1206 688 382 635
Cuts at: 4444 275
Size: 1451
Fragments arranged by size:
1451 1206 936 688 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2085 4053 4451 4725 4872 5283 2085
Size: 1968 398 274 147 411 2422
Fragments arranged by size:
2422 1968 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 2801 2831 1178
Size: 30 165 30 975 30 393 30 3967
Fragments arranged by size:
3967 975 393 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2176 2822 1186
Size: 990 646 3984
Fragments arranged by size:
3984 990 646
BseYI C'CCAG_C
Cuts at: 4823 5319 4823
Size: 496 5124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2904 3681 3766 4837 2217
Size: 687 777 85 1071 3000
Fragments arranged by size:
3000 1071 777 687 85
BsmI GAATG_Cn'
Cuts at: 3131 3224 3131
Size: 93 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2904 2939 3681 3766 4837 1391
Size: 399 427 687 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 427 399 85 35
BspEI T'CCGG_A
Cuts at: 2085 5283 2085
Size: 3198 2422
BspHI T'CATG_A
Cuts at: 2280 3509 5239 2280
Size: 1229 1730 2661
Fragments arranged by size:
2661 1730 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 3507 1394
Size: 429 467 1217 3507
Fragments arranged by size:
3507 1217 467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2078 794
Size: 1284 4336
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstXI CCAn_nnnn'nTGG
Cuts at: 2647 2647
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2053 2341 2575 2950 3317 3702 3719 5171
Size: 81 288 234 375 367 385 17 1452
Cuts at: 5171 5257 5269 5615 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 375 367 346 288 234
86 81 17 12
BstZ17I GTA'TAC
Cuts at: 2455 2455
Size: 5620
BtgI C'CryG_G
Cuts at: 513 1065 2004 2427 2816 513
Size: 552 939 423 389 3317
Fragments arranged by size:
3317 939 552 423 389
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2910 487
Size: 683 56 1684 3197
Fragments arranged by size:
3197 1684 683 56
BtsI GCAGTG_nn'
Cuts at: 2079 2202 2829 3136 3930 3950 2079
Size: 123 627 307 794 20 3749
Fragments arranged by size:
3749 794 627 307 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2433 2496 3067
Size: 54 1425 50 275 18 603 63 571
Cuts at: 3067 3956 5585 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 603 571 275 63 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2222 3550 1353
Size: 749 120 1328 3423
Fragments arranged by size:
3423 1328 749 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2902 2215
Size: 687 4933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2380 2616 3683 4280 5067 5335
Size: 393 485 263 236 1067 597 787 268
Cuts at: 5335 1239
Size: 1524
Fragments arranged by size:
1524 1067 787 597 485 393 268 263
236
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 2804
Size: 5620
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2594 2594
Size: 5620
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2131 2163 2784
Size: 32 135 32 479 32 48 32 621
Cuts at: 2784 2816 4261 4293 4335 4367 1373
Size: 32 1445 32 42 32 2626
Fragments arranged by size:
2626 1445 621 479 135 48 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2824 2964 3210 669
Size: 988 281 886 140 246 3079
Fragments arranged by size:
3079 988 886 281 246 140
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 10
Size: 54 1425 343 666 3132
Fragments arranged by size:
3132 1425 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 3578 3937 4096 518
Size: 648 1368 1044 359 159 2042
Fragments arranged by size:
2042 1368 1044 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3036 3713 4861 5106 1606
Size: 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PfoI T'CCnGG_A
Cuts at: 2415 2415
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2289 2321 3689 3721 5202 5234 2289
Size: 32 1368 32 1481 32 2675
Fragments arranged by size:
2675 1481 1368 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SacI G_AGCT'C
Cuts at: 2217 2904 2217
Size: 687 4933
SalI G'TCGA_C
Cuts at: 1936 2822 2962 1936
Size: 886 140 4594
Fragments arranged by size:
4594 886 140
SapI GCTCTTCn'nnn_
Cuts at: 2222 2222
Size: 5620
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2968 4103 4784 4975 826
Size: 219 1923 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2774 3724 4625 4887 820
Size: 197 926 831 950 901 262 1553
Fragments arranged by size:
1553 950 926 901 831 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 2379 3544 5
Size: 47 2327 1165 2081
Fragments arranged by size:
2327 2081 1165 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 3059 513
Size: 552 939 1055 3074
Fragments arranged by size:
3074 1055 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3647 3664 3817 4002 4447 325
Size: 1255 2067 17 153 185 445 1498
Fragments arranged by size:
2067 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 2801 2833 5580 5612 2378
Size: 32 391 32 2747 32 2386
Fragments arranged by size:
2747 2386 391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 2301 3749 2301
Size: 1448 4172
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2740 3428 276
Size: 53 83 186 936 1206 688 2468
Fragments arranged by size:
2468 1206 936 688 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI