Restriction map for
pFN21AB6404
(Circular) (Six-base) MAPSORT of: pFN21AB6404.seq Check: 362 from: 1 to: 5707
pFN21AB6404 5707 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2642 2738 2771 2852 3770 5154 5422
Size: 767 243 96 33 81 918 1384 268
Cuts at: 5422 1632
Size: 1917
Fragments arranged by size:
1917 1384 918 767 268 243 96 81
33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2620 3450 5022 2620
Size: 830 1572 3305
Fragments arranged by size:
3305 1572 830
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2159 2214 2237 3016 3242 3394 5503 1493
Size: 666 55 23 779 226 152 2109 1697
Fragments arranged by size:
2109 1697 779 666 226 152 55 23
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2356 3032 1870
Size: 73 84 329 676 4545
Fragments arranged by size:
4545 676 329 84 73
BamHI G'GATC_C
Cuts at: 2444 2518 2552 3037 3404 2444
Size: 74 34 485 367 4747
Fragments arranged by size:
4747 485 367 74 34
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 4483 618
Size: 325 217 627 1241 1455 1842
Fragments arranged by size:
1842 1455 1241 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2357 3026 1391
Size: 966 669 4072
Fragments arranged by size:
4072 966 669
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2630 2759 928
Size: 971 731 129 3876
Fragments arranged by size:
3876 971 731 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4924 1790
Size: 1978 1156 2573
Fragments arranged by size:
2573 1978 1156
BmgBI CAC'GTC
Cuts at: 1318 2350 1318
Size: 1032 4675
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2416 3438 4396
Size: 416 53 83 399 24 999 1022 958
Cuts at: 4396 442
Size: 1753
Fragments arranged by size:
1753 1022 999 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4367 1239
Size: 3128 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 3832 4697 4995 2480
Size: 1352 865 298 3192
Fragments arranged by size:
3192 1352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2024 2588 4370 882
Size: 617 525 564 1782 2219
Fragments arranged by size:
2219 1782 617 564 525
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 4863
BseYI C'CCAG_C
Cuts at: 2266 4910 5406 2266
Size: 2644 496 2567
Fragments arranged by size:
2644 2567 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2532 3157 3918 4946 1540
Size: 275 186 531 625 761 1028 2301
Fragments arranged by size:
2301 1028 761 625 531 275 186
BsiHKAI G_wGCw'C
Cuts at: 2050 2357 3768 3853 4924 2050
Size: 307 1411 85 1071 2833
Fragments arranged by size:
2833 1411 1071 307 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2357 3026 3768 3853 4924 1391
Size: 399 260 307 669 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 399 307 260 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 2884 96
Size: 2788 2919
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2444 2518 2552 3037 3404 3789 3806 5258
Size: 472 74 34 485 367 385 17 1452
Cuts at: 5258 5344 5356 5702 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 485 472 385 367 346 86
74 34 17 12
Bsu36I CC'TnA_GG
Cuts at: 2608 2608
Size: 5707
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2935 487
Size: 683 56 1709 3259
Fragments arranged by size:
3259 1709 683 56
BtsI GCAGTG_nn'
Cuts at: 2151 3223 4017 4037 2151
Size: 1072 794 20 3821
Fragments arranged by size:
3821 1072 794 20
ClaI AT'CG_AT
Cuts at: 1554 2634 1554
Size: 1080 4627
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2529 2694
Size: 54 1425 50 275 18 301 398 165
Cuts at: 2694 2764 3154 4043 5672 8
Size: 70 390 889 1629 43
Fragments arranged by size:
1629 1425 889 398 390 301 275 165
70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2529 3154 1537
Size: 275 717 625 4090
Fragments arranged by size:
4090 717 625 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2990 3637 1353
Size: 1637 647 3423
Fragments arranged by size:
3423 1637 647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2059 4294 4668 4814 387
Size: 1064 138 470 2235 374 146 1280
Fragments arranged by size:
2235 1280 1064 470 374 146 138
EcoICRI GAG'CTC
Cuts at: 2355 2355
Size: 5707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2642 2738 2771 2852 3770 4367
Size: 393 767 243 96 33 81 918 597
Cuts at: 4367 5154 5422 1239
Size: 787 268 1524
Fragments arranged by size:
1524 918 787 767 597 393 268 243
96 81 33
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 2510 2542 2591
Size: 32 135 32 767 32 139 32 49
Cuts at: 2591 2623 4348 4380 4422 4454 1373
Size: 32 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 767 139 135 49 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 4821 5005 1305
Size: 1146 2370 184 2007
Fragments arranged by size:
2370 2007 1146 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2133 2766 10
Size: 54 1425 343 301 633 2951
Fragments arranged by size:
2951 1425 633 343 301 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2772 2907 3665 4024 4183 518
Size: 648 1606 135 758 359 159 2042
Fragments arranged by size:
2042 1606 758 648 359 159 135
MspA1I CmG'CkG
Cuts at: 1606 3123 3800 4948 5193 1606
Size: 1517 677 1148 245 2120
Fragments arranged by size:
2120 1517 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 5675
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
SacI G_AGCT'C
Cuts at: 2357 2357
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 2731 3055 4190 4871 5062 826
Size: 219 1686 324 1135 681 191 1471
Fragments arranged by size:
1686 1471 1135 681 324 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2495 3811 4712 4974 820
Size: 197 926 552 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 552 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 3146 513
Size: 552 939 1142 3074
Fragments arranged by size:
3074 1142 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2048 2949 3734 3751 3904 4089 4534
Size: 1255 468 901 785 17 153 185 445
Cuts at: 4534 325
Size: 1498
Fragments arranged by size:
1498 1255 901 785 468 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2884 3953 5665
Size: 274 80 33 51 1443 907 1069 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1443 1069 907 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI