(Circular) (Six-base) MAPSORT of: pFN21AB6404.seq Check: 362 from: 1 to: 5707 pFN21AB6404 5707 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2642 2738 2771 2852 3770 5154 5422 Size: 767 243 96 33 81 918 1384 268 Cuts at: 5422 1632 Size: 1917 Fragments arranged by size: 1917 1384 918 767 268 243 96 81 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2620 3450 5022 2620 Size: 830 1572 3305 Fragments arranged by size: 3305 1572 830 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2159 2214 2237 3016 3242 3394 5503 1493 Size: 666 55 23 779 226 152 2109 1697 Fragments arranged by size: 2109 1697 779 666 226 152 55 23 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2027 2356 3032 1870 Size: 73 84 329 676 4545 Fragments arranged by size: 4545 676 329 84 73 BamHI G'GATC_C Cuts at: 2444 2518 2552 3037 3404 2444 Size: 74 34 485 367 4747 Fragments arranged by size: 4747 485 367 74 34 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3028 4483 618 Size: 325 217 627 1241 1455 1842 Fragments arranged by size: 1842 1455 1241 627 325 217 BanII G_rGCy'C Cuts at: 1391 2357 3026 1391 Size: 966 669 4072 Fragments arranged by size: 4072 966 669 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2630 2759 928 Size: 971 731 129 3876 Fragments arranged by size: 3876 971 731 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 3768 4924 1790 Size: 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 BmgBI CAC'GTC Cuts at: 1318 2350 1318 Size: 1032 4675 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2416 3438 4396 Size: 416 53 83 399 24 999 1022 958 Cuts at: 4396 442 Size: 1753 Fragments arranged by size: 1753 1022 999 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4367 1239 Size: 3128 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2480 3832 4697 4995 2480 Size: 1352 865 298 3192 Fragments arranged by size: 3192 1352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2024 2588 4370 882 Size: 617 525 564 1782 2219 Fragments arranged by size: 2219 1782 617 564 525 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 1186 Size: 844 4863 BseYI C'CCAG_C Cuts at: 2266 4910 5406 2266 Size: 2644 496 2567 Fragments arranged by size: 2644 2567 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2532 3157 3918 4946 1540 Size: 275 186 531 625 761 1028 2301 Fragments arranged by size: 2301 1028 761 625 531 275 186 BsiHKAI G_wGCw'C Cuts at: 2050 2357 3768 3853 4924 2050 Size: 307 1411 85 1071 2833 Fragments arranged by size: 2833 1411 1071 307 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2357 3026 3768 3853 4924 1391 Size: 399 260 307 669 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 399 307 260 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 2884 96 Size: 2788 2919 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2444 2518 2552 3037 3404 3789 3806 5258 Size: 472 74 34 485 367 385 17 1452 Cuts at: 5258 5344 5356 5702 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 485 472 385 367 346 86 74 34 17 12 Bsu36I CC'TnA_GG Cuts at: 2608 2608 Size: 5707 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2935 487 Size: 683 56 1709 3259 Fragments arranged by size: 3259 1709 683 56 BtsI GCAGTG_nn' Cuts at: 2151 3223 4017 4037 2151 Size: 1072 794 20 3821 Fragments arranged by size: 3821 1072 794 20 ClaI AT'CG_AT Cuts at: 1554 2634 1554 Size: 1080 4627 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2131 2529 2694 Size: 54 1425 50 275 18 301 398 165 Cuts at: 2694 2764 3154 4043 5672 8 Size: 70 390 889 1629 43 Fragments arranged by size: 1629 1425 889 398 390 301 275 165 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2529 3154 1537 Size: 275 717 625 4090 Fragments arranged by size: 4090 717 625 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2990 3637 1353 Size: 1637 647 3423 Fragments arranged by size: 3423 1637 647 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2059 4294 4668 4814 387 Size: 1064 138 470 2235 374 146 1280 Fragments arranged by size: 2235 1280 1064 470 374 146 138 EcoICRI GAG'CTC Cuts at: 2355 2355 Size: 5707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2642 2738 2771 2852 3770 4367 Size: 393 767 243 96 33 81 918 597 Cuts at: 4367 5154 5422 1239 Size: 787 268 1524 Fragments arranged by size: 1524 918 787 767 597 393 268 243 96 81 33 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 2510 2542 2591 Size: 32 135 32 767 32 139 32 49 Cuts at: 2591 2623 4348 4380 4422 4454 1373 Size: 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 767 139 135 49 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2451 4821 5005 1305 Size: 1146 2370 184 2007 Fragments arranged by size: 2370 2007 1146 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2133 2766 10 Size: 54 1425 343 301 633 2951 Fragments arranged by size: 2951 1425 633 343 301 54 MslI CAynn'nnrTG Cuts at: 518 1166 2772 2907 3665 4024 4183 518 Size: 648 1606 135 758 359 159 2042 Fragments arranged by size: 2042 1606 758 648 359 159 135 MspA1I CmG'CkG Cuts at: 1606 3123 3800 4948 5193 1606 Size: 1517 677 1148 245 2120 Fragments arranged by size: 2120 1517 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2669 2701 2669 Size: 32 5675 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 SacI G_AGCT'C Cuts at: 2357 2357 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 2731 3055 4190 4871 5062 826 Size: 219 1686 324 1135 681 191 1471 Fragments arranged by size: 1686 1471 1135 681 324 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2495 3811 4712 4974 820 Size: 197 926 552 1316 901 262 1553 Fragments arranged by size: 1553 1316 926 901 552 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 3146 513 Size: 552 939 1142 3074 Fragments arranged by size: 3074 1142 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2048 2949 3734 3751 3904 4089 4534 Size: 1255 468 901 785 17 153 185 445 Cuts at: 4534 325 Size: 1498 Fragments arranged by size: 1498 1255 901 785 468 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2884 3953 5665 Size: 274 80 33 51 1443 907 1069 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1712 1443 1069 907 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5667 5699 5667 Size: 32 5675 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI