Restriction map for
pFN21AB6402
(Circular) (Six-base) MAPSORT of: pFN21AB6402.seq Check: 5076 from: 1 to: 5704
pFN21AB6402 5704 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2263 2263
Size: 5704
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2505 3047 1937
Size: 568 542 4594
Fragments arranged by size:
4594 568 542
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3767 5151 5419 1632
Size: 2135 1384 268 1917
Fragments arranged by size:
2135 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 2500 3447 5019 2500
Size: 947 1572 3185
Fragments arranged by size:
3185 1572 947
ApaI G_GGCC'C
Cuts at: 2736 2736
Size: 5704
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 2264 3013 3239 3391 5500 1493
Size: 771 749 226 152 2109 1697
Fragments arranged by size:
2109 1697 771 749 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3025 4480 618
Size: 325 217 627 1238 1455 1842
Fragments arranged by size:
1842 1455 1238 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2736 3023 1391
Size: 1345 287 4072
Fragments arranged by size:
4072 1345 287
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2826 928
Size: 971 927 3806
Fragments arranged by size:
3806 971 927
BbvCI CC'TCA_GC
Cuts at: 2050 2980 2050
Size: 930 4774
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 2068 1548
Size: 520 5184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4315 136
Size: 107 122 71 631 605 2643 1525
Fragments arranged by size:
2643 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2351 5699 2351
Size: 3348 2356
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 2736 3765 4921 1790
Size: 946 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 946
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2478 3435 4393
Size: 416 53 83 399 24 1061 957 958
Cuts at: 4393 442
Size: 1753
Fragments arranged by size:
1753 1061 958 957 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4364 1239
Size: 3125 2579
Bpu10I CC'TnA_GC
Cuts at: 2050 2691 2980 2050
Size: 641 289 4774
Fragments arranged by size:
4774 641 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2505 3829 4694 4992 2505
Size: 1324 865 298 3217
Fragments arranged by size:
3217 1324 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2380 2938 3495 493
Size: 711 1176 558 557 2702
Fragments arranged by size:
2702 1176 711 558 557
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3511 3893 4528 275
Size: 53 83 186 936 1978 382 635 1451
Fragments arranged by size:
1978 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2460 2955 4137 4535 4809 4956 5367 2460
Size: 495 1182 398 274 147 411 2797
Fragments arranged by size:
2797 1182 495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 1186
Size: 975 4729
BseYI C'CCAG_C
Cuts at: 2907 4907 5403 2907
Size: 2000 496 3208
Fragments arranged by size:
3208 2000 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 1163
Size: 573 435 4696
Fragments arranged by size:
4696 573 435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2443 3765 3850 4921 2443
Size: 1322 85 1071 3226
Fragments arranged by size:
3226 1322 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2443 2736 3023 3765 3850 4921 1391
Size: 399 653 293 287 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 653 399 293 287 85
BspEI T'CCGG_A
Cuts at: 2460 5367 2460
Size: 2907 2797
BspHI T'CATG_A
Cuts at: 2761 3593 5323 2761
Size: 832 1730 3142
Fragments arranged by size:
3142 1730 832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2263 2747 3059 844
Size: 1419 484 312 3489
Fragments arranged by size:
3489 1419 484 312
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2533 2545 4199 4373 65
Size: 1183 1285 12 1654 174 1396
Fragments arranged by size:
1654 1396 1285 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2672 4348 4535 1700
Size: 110 862 1676 187 2869
Fragments arranged by size:
2869 1676 862 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5704
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2351 3034 3401 3786 3803 5255 5341 5353
Size: 379 683 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 683 385 379 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2120 487
Size: 683 56 894 4071
Fragments arranged by size:
4071 894 683 56
BtsI GCAGTG_nn'
Cuts at: 2160 2821 3220 4014 4034 2160
Size: 661 399 794 20 3830
Fragments arranged by size:
3830 794 661 399 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DraIII CAC_nnn'GTG
Cuts at: 2489 2489
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2946 3151
Size: 54 1425 50 275 18 399 717 205
Cuts at: 3151 4040 5669 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 717 399 275 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3634 1353
Size: 2281 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3767 4364 5151 5419 1239
Size: 393 2135 597 787 268 1524
Fragments arranged by size:
2135 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2711 2711
Size: 5704
EcoO109I rG'GnC_Cy
Cuts at: 2344 2704 2732 2344
Size: 360 28 5316
Fragments arranged by size:
5316 360 28
EcoRI G'AATT_C
Cuts at: 2264 3013 3391 2264
Size: 749 378 4577
Fragments arranged by size:
4577 749 378
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 4851 1678
Size: 3173 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373
Size: 32 135 32 2773 32 42 32 2626
Fragments arranged by size:
2773 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4818 5002 1305
Size: 3513 184 2007
Fragments arranged by size:
3513 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2231 2948 10
Size: 54 1425 343 399 717 2766
Fragments arranged by size:
2766 1425 717 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 3662 4021 4180 518
Size: 648 1008 1488 359 159 2042
Fragments arranged by size:
2042 1488 1008 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2907 3120 3797 4945 5190 1606
Size: 1301 213 677 1148 245 2120
Fragments arranged by size:
2120 1301 1148 677 245 213
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5152
NdeI CA'TA_TG
Cuts at: 387 2614 387
Size: 2227 3477
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PfoI T'CCnGG_A
Cuts at: 2322 2322
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PspOMI G'GGCC_C
Cuts at: 2732 2732
Size: 5704
PstI C_TGCA'G
Cuts at: 830 3056 830
Size: 2226 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
PvuII CAG'CTG
Cuts at: 2907 2907
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 2809 3052 4187 4868 5059 826
Size: 219 1764 243 1135 681 191 1471
Fragments arranged by size:
1764 1471 1135 681 243 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2520 3808 4709 4971 820
Size: 197 926 577 1288 901 262 1553
Fragments arranged by size:
1553 1288 926 901 577 262 197
SnaBI TAC'GTA
Cuts at: 493 2938 3495 493
Size: 2445 557 2702
Fragments arranged by size:
2702 2445 557
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 2888 3628 5
Size: 47 2836 740 2081
Fragments arranged by size:
2836 2081 740 47
StuI AGG'CCT
Cuts at: 1517 2589 1517
Size: 1072 4632
StyI C'CwwG_G
Cuts at: 513 1065 3143 513
Size: 552 2078 3074
Fragments arranged by size:
3074 2078 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2918 3731 3748 3901 4086 4531 325
Size: 1255 1338 813 17 153 185 445 1498
Fragments arranged by size:
1498 1338 1255 813 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2640 3950 5662
Size: 274 80 33 51 1443 663 1310 1712
Cuts at: 5662 96
Size: 138
Fragments arranged by size:
1712 1443 1310 663 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2318 3833 2318
Size: 1515 4189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI