Restriction map for
pFN21AB6387
(Circular) (Six-base) MAPSORT of: pFN21AB6387.seq Check: 5923 from: 1 to: 5530
pFN21AB6387 5530 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2789 3340 278
Size: 53 83 186 936 1253 551 2468
Fragments arranged by size:
2468 1253 936 551 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 1937
Size: 936 4594
Acc65I G'GTAC_C
Cuts at: 2851 2851
Size: 5530
AclI AA'CG_TT
Cuts at: 1563 3657 4030 1563
Size: 2094 373 3063
Fragments arranged by size:
3063 2094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3593 4977 5245 1632
Size: 1961 1384 268 1917
Fragments arranged by size:
1961 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5333
AflIII A'CryG_T
Cuts at: 1203 4429 5406 1203
Size: 3226 977 1327
Fragments arranged by size:
3226 1327 977
AgeI A'CCGG_T
Cuts at: 4361 4361
Size: 5530
AhdI GACnn_n'nnGTC
Cuts at: 4259 4259
Size: 5530
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5530
AlwNI CAG_nnn'CTG
Cuts at: 3273 4845 3273
Size: 1572 3958
ApaLI G'TGCA_C
Cuts at: 3587 4743 3587
Size: 1156 4374
ApoI r'AATT_y
Cuts at: 1493 2839 3065 3217 5326 1493
Size: 1346 226 152 2109 1697
Fragments arranged by size:
2109 1697 1346 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4084 160
Size: 872 3052 1606
Fragments arranged by size:
3052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2855 1870
Size: 73 149 763 4545
Fragments arranged by size:
4545 763 149 73
BamHI G'GATC_C
Cuts at: 2860 3227 2860
Size: 367 5163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 4306 618
Size: 325 217 627 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2277 2849 1391
Size: 886 572 4072
Fragments arranged by size:
4072 886 572
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3721 3755 1903
Size: 34 1784 34 3678
Fragments arranged by size:
3678 1784 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3422 4632 3422
Size: 1210 4320
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4141 136
Size: 107 122 71 631 605 2469 1525
Fragments arranged by size:
2469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2485 2805 5525 2485
Size: 320 2720 2490
Fragments arranged by size:
2720 2490 320
BlpI GC'TnA_GC
Cuts at: 2947 2947
Size: 5530
Bme1580I G_kGCm'C
Cuts at: 1790 3591 4747 1790
Size: 1801 1156 2573
Fragments arranged by size:
2573 1801 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3261 4219
Size: 416 53 83 399 24 897 947 958
Cuts at: 4219 442
Size: 1753
Fragments arranged by size:
1753 958 947 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 2625 2705 2797 4190 1239
Size: 1161 225 80 92 1393 2579
Fragments arranged by size:
2579 1393 1161 225 92 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3655 4520 4818 3655
Size: 865 298 4367
Fragments arranged by size:
4367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2340 4193 882
Size: 617 841 1853 2219
Fragments arranged by size:
2219 1853 841 617
BsaAI yAC'GTr
Cuts at: 493 1204 3321 493
Size: 711 2117 2702
Fragments arranged by size:
2702 2117 711
BsaBI GATnn'nnATC
Cuts at: 5524 5524
Size: 5530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2786 3337 3719 4354
Size: 53 83 186 936 1253 551 382 635
Cuts at: 4354 275
Size: 1451
Fragments arranged by size:
1451 1253 936 635 551 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3963 4361 4635 4782 5193 3963
Size: 398 274 147 411 4300
Fragments arranged by size:
4300 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2337 2367 1178
Size: 30 165 30 741 30 163 30 4341
Fragments arranged by size:
4341 741 165 163 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 1186
Size: 928 4602
BseYI C'CCAG_C
Cuts at: 4733 5229 4733
Size: 496 5034
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2046 2166 2495 1163
Size: 573 310 120 329 4198
Fragments arranged by size:
4198 573 329 310 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2159 2257 2980 3741 4769 1540
Size: 275 186 158 98 723 761 1028 2301
Fragments arranged by size:
2301 1028 761 723 275 186 158 98
BsiHKAI G_wGCw'C
Cuts at: 2091 2186 3591 3676 4747 2091
Size: 95 1405 85 1071 2874
Fragments arranged by size:
2874 1405 1071 95 85
BsmI GAATG_Cn'
Cuts at: 2176 3041 3134 2176
Size: 865 93 4572
Fragments arranged by size:
4572 865 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2186 2277 2849 3591 3676 4747
Size: 399 301 95 91 572 742 85 1071
Cuts at: 4747 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 572 399 301 95 91
85
BspEI T'CCGG_A
Cuts at: 5193 5193
Size: 5530
BspHI T'CATG_A
Cuts at: 3419 5149 3419
Size: 1730 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2467 2885 844
Size: 1179 444 418 3489
Fragments arranged by size:
3489 1179 444 418
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 1394
Size: 429 1594 3507
Fragments arranged by size:
3507 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4025 4199 65
Size: 1183 2777 174 1396
Fragments arranged by size:
2777 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4174 4361 1700
Size: 110 2364 187 2869
Fragments arranged by size:
2869 2364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5530
BssHII G'CGCG_C
Cuts at: 1925 2054 2056 1925
Size: 129 2 5399
Fragments arranged by size:
5399 129 2
BssSI C'ACGA_G
Cuts at: 3590 4602 3590
Size: 1012 4518
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5530
BstBI TT'CG_AA
Cuts at: 4334 4334
Size: 5530
BstEII G'GTnAC_C
Cuts at: 1184 2242 1184
Size: 1058 4472
BstXI CCAn_nnnn'nTGG
Cuts at: 2357 2572 2357
Size: 215 5315
BstYI r'GATC_y
Cuts at: 1972 2485 2805 2860 3227 3612 3629 5081 5167
Size: 513 320 55 367 385 17 1452 86
Cuts at: 5167 5179 5525 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 513 385 367 346 320 86
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4039
Fragments arranged by size:
4039 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4791
Fragments arranged by size:
4791 683 56
BtsI GCAGTG_nn'
Cuts at: 2154 2287 3046 3840 3860 2154
Size: 133 759 794 20 3824
Fragments arranged by size:
3824 794 759 133 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5495
DraI TTT'AAA
Cuts at: 2605 2834 2605
Size: 229 5301
DraIII CAC_nnn'GTG
Cuts at: 2250 2250
Size: 5530
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2592 4537 809
Size: 507 1276 1945 1802
Fragments arranged by size:
1945 1802 1276 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2254 2497
Size: 54 1425 50 275 18 177 247 243
Cuts at: 2497 2977 3866 5495 8
Size: 480 889 1629 43
Fragments arranged by size:
1629 1425 889 480 275 247 243 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2254 2977 1537
Size: 275 442 723 4090
Fragments arranged by size:
4090 723 442 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3460 1353
Size: 2107 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4117 4491 4637 387
Size: 1064 138 2528 374 146 1280
Fragments arranged by size:
2528 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2625 2705 2797 3593 4190 4977
Size: 393 768 225 80 92 796 597 787
Cuts at: 4977 5245 1239
Size: 268 1524
Fragments arranged by size:
1524 796 787 768 597 393 268 225
92 80
EcoO109I rG'GnC_Cy
Cuts at: 2135 2135
Size: 5530
EcoRI G'AATT_C
Cuts at: 2839 3217 2839
Size: 378 5152
FspI TGC'GCA
Cuts at: 4036 4036
Size: 5530
HaeII r_GCGC'y
Cuts at: 1678 2224 4677 1678
Size: 546 2453 2531
Fragments arranged by size:
2531 2453 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2475 2507 2776 2808 4171
Size: 32 135 32 903 32 269 32 1363
Cuts at: 4171 4203 4245 4277 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1363 903 269 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2874 3120 669
Size: 988 281 936 246 3079
Fragments arranged by size:
3079 988 936 281 246
HindIII A'AGCT_T
Cuts at: 748 2769 748
Size: 2021 3509
HpaI GTT'AAC
Cuts at: 3120 3120
Size: 5530
KpnI G_GTAC'C
Cuts at: 2855 2855
Size: 5530
MfeI C'AATT_G
Cuts at: 3129 3129
Size: 5530
MluI A'CGCG_T
Cuts at: 5406 5406
Size: 5530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2383 2461 4644 4828 1305
Size: 1078 78 2183 184 2007
Fragments arranged by size:
2183 2007 1078 184 78
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2499 10
Size: 54 1425 343 667 3041
Fragments arranged by size:
3041 1425 667 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3488 3847 4006 518
Size: 648 2322 359 159 2042
Fragments arranged by size:
2322 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2289 2553 2946 3623 4771 5016 1606
Size: 683 264 393 677 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 393 264 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4039
Fragments arranged by size:
4039 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5530
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5530
NotI GC'GGCC_GC
Cuts at: 2254 2977 2254
Size: 723 4807
NspI r_CATG'y
Cuts at: 1132 1229 2888 4433 1132
Size: 97 1659 1545 2229
Fragments arranged by size:
2229 1659 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2138 1348
Size: 790 4740
PciI A'CATG_T
Cuts at: 4429 4429
Size: 5530
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2506 1108
Size: 1398 4132
PmeI GTTT'AAAC
Cuts at: 2834 2834
Size: 5530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 5112 5144 3599
Size: 32 1481 32 3985
Fragments arranged by size:
3985 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5530
PsiI TTA'TAA
Cuts at: 3100 3100
Size: 5530
PstI C_TGCA'G
Cuts at: 830 2882 830
Size: 2052 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5530
PvuII CAG'CTG
Cuts at: 2553 2553
Size: 5530
RsrII CG'GwC_CG
Cuts at: 2207 2207
Size: 5530
SalI G'TCGA_C
Cuts at: 1936 2872 1936
Size: 936 4594
SbfI CC_TGCA'GG
Cuts at: 2882 2882
Size: 5530
ScaI AGT'ACT
Cuts at: 2073 3778 5490 2073
Size: 1705 1712 2113
Fragments arranged by size:
2113 1712 1705
SfcI C'TryA_G
Cuts at: 826 1045 2878 4013 4694 4885 826
Size: 219 1833 1135 681 191 1471
Fragments arranged by size:
1833 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5530
SmaI CCC'GGG
Cuts at: 1872 2094 2857 1872
Size: 222 763 4545
Fragments arranged by size:
4545 763 222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3634 4535 4797 820
Size: 197 926 1691 901 262 1553
Fragments arranged by size:
1691 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3321 493
Size: 2828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5530
SphI G_CATG'C
Cuts at: 1132 2888 1132
Size: 1756 3774
SspI AAT'ATT
Cuts at: 5 52 3454 5
Size: 47 3402 2081
Fragments arranged by size:
3402 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5530
StyI C'CwwG_G
Cuts at: 513 1065 2004 2969 513
Size: 552 939 965 3074
Fragments arranged by size:
3074 965 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2296 2564 3557 3574 3727 3912 4357
Size: 1255 716 268 993 17 153 185 445
Cuts at: 4357 325
Size: 1498
Fragments arranged by size:
1498 1255 993 716 445 268 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2071 3776 5488
Size: 274 80 33 51 1443 94 1705 1712
Cuts at: 5488 96
Size: 138
Fragments arranged by size:
1712 1705 1443 274 138 94 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2337 2369 5490 5522 2337
Size: 32 3121 32 2345
Fragments arranged by size:
3121 2345 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5530
XbaI T'CTAG_A
Cuts at: 2866 2866
Size: 5530
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5530
XmaI C'CCGG_G
Cuts at: 1870 2092 2855 1870
Size: 222 763 4545
Fragments arranged by size:
4545 763 222
XmnI GAAnn'nnTTC
Cuts at: 3659 3659
Size: 5530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2787 3338 276
Size: 53 83 186 936 1253 551 2468
Fragments arranged by size:
2468 1253 936 551 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI