Restriction map for
pFN21AB6386
(Circular) (Six-base) MAPSORT of: pFN21AB6386.seq Check: 6472 from: 1 to: 5608
pFN21AB6386 5608 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2453 2453
Size: 5608
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2663 2951 1937
Size: 726 288 4594
Fragments arranged by size:
4594 726 288
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3671 5055 5323 1632
Size: 2039 1384 268 1917
Fragments arranged by size:
2039 1917 1384 268
AfeI AGC'GCT
Cuts at: 2135 2135
Size: 5608
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 2459 3351 4923 2459
Size: 892 1572 3144
Fragments arranged by size:
3144 1572 892
ApaI G_GGCC'C
Cuts at: 2446 2446
Size: 5608
ApaLI G'TGCA_C
Cuts at: 2701 3665 4821 2701
Size: 964 1156 3488
Fragments arranged by size:
3488 1156 964
ApoI r'AATT_y
Cuts at: 1493 2830 2917 3143 3295 5404 1493
Size: 1337 87 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152 87
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 1870
Size: 73 990 4545
Fragments arranged by size:
4545 990 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2783 2929 4384 618
Size: 325 217 627 996 146 1455 1842
Fragments arranged by size:
1842 1455 996 627 325 217 146
BanII G_rGCy'C
Cuts at: 1391 2302 2446 2927 1391
Size: 911 144 481 4072
Fragments arranged by size:
4072 911 481 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2766 928
Size: 971 867 3770
Fragments arranged by size:
3770 971 867
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2692 2726 3799 3833 1903
Size: 34 755 34 1073 34 3678
Fragments arranged by size:
3678 1073 755 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2446 2705 3669 4825 1790
Size: 656 259 964 1156 2573
Fragments arranged by size:
2573 1156 964 656 259
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2082 3339 4297
Size: 416 53 83 399 24 665 1257 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1257 958 665 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2624 1056
Size: 1568 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2702 2765 2855 4268 1239
Size: 1178 285 63 90 1413 2579
Fragments arranged by size:
2579 1413 1178 285 90 63
Bpu10I CC'TnA_GC
Cuts at: 2301 2301
Size: 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2419 4271 882
Size: 617 920 1852 2219
Fragments arranged by size:
2219 1852 920 617
BsaAI yAC'GTr
Cuts at: 493 1204 2047 2638 2816 3399 493
Size: 711 843 591 178 583 2702
Fragments arranged by size:
2702 843 711 591 583 178
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2433 4041 4439 4713 4860 5271 2433
Size: 1608 398 274 147 411 2770
Fragments arranged by size:
2770 1608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2810 1186
Size: 1033 591 3984
Fragments arranged by size:
3984 1033 591
BseYI C'CCAG_C
Cuts at: 2836 4811 5307 2836
Size: 1975 496 3137
Fragments arranged by size:
3137 1975 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5035
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2155 2302 2705 2721 3669 3754 4825 2155
Size: 147 403 16 948 85 1071 2938
Fragments arranged by size:
2938 1071 948 403 147 85 16
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2302 2446 2705 2721 2927 3669
Size: 399 365 147 144 259 16 206 742
Cuts at: 3669 3754 4825 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 365 259 206 147
144 85 16
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2453 2963 844
Size: 1609 510 3489
Fragments arranged by size:
3489 1609 510
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2503 4103 4277 65
Size: 1183 1255 1600 174 1396
Fragments arranged by size:
1600 1396 1255 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2142 4252 4439 1700
Size: 110 332 2110 187 2869
Fragments arranged by size:
2869 2110 332 187 110
BsrGI T'GTAC_A
Cuts at: 96 2425 96
Size: 2329 3279
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2459 2630 794
Size: 1665 171 3772
Fragments arranged by size:
3772 1665 171
BstBI TT'CG_AA
Cuts at: 2712 4412 2712
Size: 1700 3908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstXI CCAn_nnnn'nTGG
Cuts at: 2167 2167
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2400 2860 2938 3305 3690 3707 5159 5245
Size: 428 460 78 367 385 17 1452 86
Cuts at: 5245 5257 5603 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 460 428 385 367 346 86
78 17 12
BstZ17I GTA'TAC
Cuts at: 2664 2664
Size: 5608
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 513
Size: 552 939 162 3955
Fragments arranged by size:
3955 939 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2464 487
Size: 683 56 1238 3631
Fragments arranged by size:
3631 1238 683 56
BtsI GCAGTG_nn'
Cuts at: 2883 3124 3918 3938 2883
Size: 241 794 20 4553
Fragments arranged by size:
4553 794 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 3055 3944
Size: 54 1425 50 275 18 273 952 889
Cuts at: 3944 5573 8
Size: 1629 43
Fragments arranged by size:
1629 1425 952 889 275 273 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 2709 3538 1353
Size: 1211 145 829 3423
Fragments arranged by size:
3423 1211 829 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2186 4195 4569 4715 387
Size: 1064 138 597 2009 374 146 1280
Fragments arranged by size:
2009 1280 1064 597 374 146 138
EcoICRI GAG'CTC
Cuts at: 2300 2300
Size: 5608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2702 2765 2855 3671 4268 5055
Size: 393 785 285 63 90 816 597 787
Cuts at: 5055 5323 1239
Size: 268 1524
Fragments arranged by size:
1524 816 787 785 597 393 285 268
90 63
EcoO109I rG'GnC_Cy
Cuts at: 2116 2443 2749 2116
Size: 327 306 4975
Fragments arranged by size:
4975 327 306
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 5608
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2137 4755 1678
Size: 459 2618 2531
Fragments arranged by size:
2618 2531 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2689 2721 4249 4281 4323
Size: 32 135 32 1117 32 1528 32 42
Cuts at: 4323 4355 1373
Size: 32 2626
Fragments arranged by size:
2626 1528 1117 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2184 4722 4906 1305
Size: 879 2538 184 2007
Fragments arranged by size:
2538 2007 879 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 3925 4084 518
Size: 648 2400 359 159 2042
Fragments arranged by size:
2400 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2368 2539 3024 3701 4849 5094 1606
Size: 762 171 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 485 245 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 2166 513
Size: 552 939 162 3955
Fragments arranged by size:
3955 939 552 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 2620 1052
Size: 1568 4040
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PmlI CAC'GTG
Cuts at: 2047 2047
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2749 2749
Size: 5608
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2442 2442
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2464 2960 830
Size: 1634 496 3478
Fragments arranged by size:
3478 1634 496
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2539 2539
Size: 5608
SacI G_AGCT'C
Cuts at: 2302 2302
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SapI GCTCTTCn'nnn_
Cuts at: 2709 2709
Size: 5608
SbfI CC_TGCA'GG
Cuts at: 2464 2960 2464
Size: 496 5112
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2460 2956 4091 4772 4963 826
Size: 219 1415 496 1135 681 191 1471
Fragments arranged by size:
1471 1415 1135 681 496 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3712 4613 4875 820
Size: 197 926 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2816 3399 493
Size: 2323 583 2702
Fragments arranged by size:
2702 2323 583
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 3047 513
Size: 552 939 162 881 3074
Fragments arranged by size:
3074 939 881 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2736 3635 3652 3805 3990 4435 325
Size: 1255 1156 899 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1156 899 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2425 3854 5566
Size: 274 80 33 51 1443 448 1429 1712
Cuts at: 5566 96
Size: 138
Fragments arranged by size:
1712 1443 1429 448 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2355 2387 5568 5600 2355
Size: 32 3181 32 2363
Fragments arranged by size:
3181 2363 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 2831 3737 2831
Size: 906 4702
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI