Restriction map for
pFN21AB6379
(Circular) (Six-base) MAPSORT of: pFN21AB6379.seq Check: 2938 from: 1 to: 5389
pFN21AB6379 5389 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2315 2315
Size: 5389
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2025 3199 278
Size: 53 83 186 936 489 1174 2468
Fragments arranged by size:
2468 1174 936 489 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2732 1937
Size: 795 4594
Acc65I G'GTAC_C
Cuts at: 2710 2710
Size: 5389
AclI AA'CG_TT
Cuts at: 1563 3516 3889 1563
Size: 1953 373 3063
Fragments arranged by size:
3063 1953 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 3452 4836 5104 1632
Size: 701 1119 1384 268 1917
Fragments arranged by size:
1917 1384 1119 701 268
AflII C'TTAA_G
Cuts at: 820 1017 2113 2365 820
Size: 197 1096 252 3844
Fragments arranged by size:
3844 1096 252 197
AflIII A'CryG_T
Cuts at: 1203 4288 5265 1203
Size: 3085 977 1327
Fragments arranged by size:
3085 1327 977
AgeI A'CCGG_T
Cuts at: 4220 4220
Size: 5389
AhdI GACnn_n'nnGTC
Cuts at: 4118 4118
Size: 5389
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5389
AlwNI CAG_nnn'CTG
Cuts at: 2185 3132 4704 2185
Size: 947 1572 2870
Fragments arranged by size:
2870 1572 947
ApaLI G'TGCA_C
Cuts at: 3446 4602 3446
Size: 1156 4233
ApoI r'AATT_y
Cuts at: 1493 2057 2153 2531 2698 2924 3076 5185 1493
Size: 564 96 378 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 564 378 226 167 152 96
AseI AT'TA_AT
Cuts at: 160 1032 3943 160
Size: 872 2911 1606
Fragments arranged by size:
2911 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
AvaI C'yCGr_G
Cuts at: 1870 1943 2714 1870
Size: 73 771 4545
Fragments arranged by size:
4545 771 73
BamHI G'GATC_C
Cuts at: 2719 3086 2719
Size: 367 5022
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2549 2710 4165 618
Size: 325 217 627 762 161 1455 1842
Fragments arranged by size:
1842 1455 762 627 325 217 161
BanII G_rGCy'C
Cuts at: 1391 2708 1391
Size: 1317 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3580 3614 1903
Size: 34 1643 34 3678
Fragments arranged by size:
3678 1643 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3281 4491 3281
Size: 1210 4179
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5389
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4000 136
Size: 107 122 71 631 605 337 1991 1525
Fragments arranged by size:
1991 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2359 5384 2359
Size: 3025 2364
BlpI GC'TnA_GC
Cuts at: 2270 2806 2270
Size: 536 4853
Bme1580I G_kGCm'C
Cuts at: 1790 2552 3450 4606 1790
Size: 762 898 1156 2573
Fragments arranged by size:
2573 1156 898 762
BmgBI CAC'GTC
Cuts at: 1318 2029 1318
Size: 711 4678
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3120 4078 442
Size: 416 53 83 399 24 1703 958 1753
Fragments arranged by size:
1753 1703 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2633 1056
Size: 1577 3812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2378 2378
Size: 5389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2207 2222 4049 1239
Size: 848 120 15 1827 2579
Fragments arranged by size:
2579 1827 848 120 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3514 4379 4677 3514
Size: 865 298 4226
Fragments arranged by size:
4226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4052 882
Size: 617 2553 2219
Fragments arranged by size:
2553 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3180 493
Size: 711 1976 2702
Fragments arranged by size:
2702 1976 711
BsaBI GATnn'nnATC
Cuts at: 5383 5383
Size: 5389
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 3196 3578 4213
Size: 53 83 186 936 489 1174 382 635
Cuts at: 4213 275
Size: 1451
Fragments arranged by size:
1451 1174 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2031 3822 4220 4494 4641 5052 2031
Size: 1791 398 274 147 411 2368
Fragments arranged by size:
2368 1791 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 1178
Size: 30 165 30 817 30 4317
Fragments arranged by size:
4317 817 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2370 2489 1186
Size: 1184 119 4086
Fragments arranged by size:
4086 1184 119
BseYI C'CCAG_C
Cuts at: 2454 2560 4592 5088 2454
Size: 106 2032 496 2755
Fragments arranged by size:
2755 2032 496 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4816
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2839 3600 4628 1540
Size: 275 186 838 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2122 3450 3535 4606 2122
Size: 1328 85 1071 2905
Fragments arranged by size:
2905 1328 1071 85
BsmI GAATG_Cn'
Cuts at: 2900 2993 2900
Size: 93 5296
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2552 2708 3450 3535 4606 1391
Size: 399 332 430 156 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 430 399 332 156 85
BspEI T'CCGG_A
Cuts at: 2031 5052 2031
Size: 3021 2368
BspHI T'CATG_A
Cuts at: 3278 5008 3278
Size: 1730 3659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2315 2449 2744 844
Size: 1471 134 295 3489
Fragments arranged by size:
3489 1471 295 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3276 1394
Size: 429 1453 3507
Fragments arranged by size:
3507 1453 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3884 4058 65
Size: 1183 2636 174 1396
Fragments arranged by size:
2636 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4033 4220 1700
Size: 110 2223 187 2869
Fragments arranged by size:
2869 2223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5389
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5389
BssSI C'ACGA_G
Cuts at: 3449 4461 3449
Size: 1012 4377
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5389
BstBI TT'CG_AA
Cuts at: 4193 4193
Size: 5389
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5389
BstXI CCAn_nnnn'nTGG
Cuts at: 2560 2560
Size: 5389
BstYI r'GATC_y
Cuts at: 1972 2034 2359 2719 3086 3471 3488 4940 5026
Size: 62 325 360 367 385 17 1452 86
Cuts at: 5026 5038 5384 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 360 346 325 86
62 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3898
Fragments arranged by size:
3898 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4650
Fragments arranged by size:
4650 683 56
BtsI GCAGTG_nn'
Cuts at: 2905 3699 3719 2905
Size: 794 20 4575
Fragments arranged by size:
4575 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5354
DraI TTT'AAA
Cuts at: 2693 2693
Size: 5389
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2027 4396 809
Size: 507 711 2369 1802
Fragments arranged by size:
2369 1802 711 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2836 3725 5354
Size: 54 1425 50 275 18 1006 889 1629
Cuts at: 5354 8
Size: 43
Fragments arranged by size:
1629 1425 1006 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2836 1537
Size: 275 1024 4090
Fragments arranged by size:
4090 1024 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3319 1353
Size: 1966 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3976 4350 4496 387
Size: 1064 138 2387 374 146 1280
Fragments arranged by size:
2387 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2207 2222 2333 3452 4049 4836
Size: 393 455 120 15 111 1119 597 787
Cuts at: 4836 5104 1239
Size: 268 1524
Fragments arranged by size:
1524 1119 787 597 455 393 268 120
111 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2287 2287
Size: 5389
EcoO109I rG'GnC_Cy
Cuts at: 2108 2182 2108
Size: 74 5315
EcoRI G'AATT_C
Cuts at: 2698 3076 2698
Size: 378 5011
EcoRV GAT'ATC
Cuts at: 2092 2092
Size: 5389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2360 2692 2360
Size: 332 5057
FspI TGC'GCA
Cuts at: 3895 3895
Size: 5389
HaeII r_GCGC'y
Cuts at: 1678 2081 4536 1678
Size: 403 2455 2531
Fragments arranged by size:
2531 2455 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 4030 4062 4104
Size: 32 135 32 629 32 1797 32 42
Cuts at: 4104 4136 1373
Size: 32 2626
Fragments arranged by size:
2626 1797 629 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2733 2979 669
Size: 988 281 795 246 3079
Fragments arranged by size:
3079 988 795 281 246
HindIII A'AGCT_T
Cuts at: 748 2570 2609 748
Size: 1822 39 3528
Fragments arranged by size:
3528 1822 39
HpaI GTT'AAC
Cuts at: 2979 2979
Size: 5389
KpnI G_GTAC'C
Cuts at: 2714 2714
Size: 5389
MfeI C'AATT_G
Cuts at: 2988 2988
Size: 5389
MluI A'CGCG_T
Cuts at: 5265 5265
Size: 5389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4503 4687 1305
Size: 3198 184 2007
Fragments arranged by size:
3198 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3567
Fragments arranged by size:
3567 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3347 3706 3865 518
Size: 648 2181 359 159 2042
Fragments arranged by size:
2181 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2560 2805 3482 4630 4875 1606
Size: 954 245 677 1148 245 2120
Fragments arranged by size:
2120 1148 954 677 245 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3898
Fragments arranged by size:
3898 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5389
NheI G'CTAG_C
Cuts at: 1052 2629 1052
Size: 1577 3812
NotI GC'GGCC_GC
Cuts at: 2836 2836
Size: 5389
NspI r_CATG'y
Cuts at: 1132 1229 2747 4292 1132
Size: 97 1518 1545 2229
Fragments arranged by size:
2229 1545 1518 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5389
PciI A'CATG_T
Cuts at: 4288 4288
Size: 5389
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 1108
Size: 1137 4252
PfoI T'CCnGG_A
Cuts at: 2238 2514 2238
Size: 276 5113
PmeI GTTT'AAAC
Cuts at: 2693 2693
Size: 5389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 4971 5003 3458
Size: 32 1481 32 3844
Fragments arranged by size:
3844 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2108 2182 2108
Size: 74 5315
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5389
PsiI TTA'TAA
Cuts at: 2959 2959
Size: 5389
PstI C_TGCA'G
Cuts at: 830 2741 830
Size: 1911 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5389
PvuII CAG'CTG
Cuts at: 2560 2560
Size: 5389
SalI G'TCGA_C
Cuts at: 1936 2731 1936
Size: 795 4594
SbfI CC_TGCA'GG
Cuts at: 2741 2741
Size: 5389
ScaI AGT'ACT
Cuts at: 3637 5349 3637
Size: 1712 3677
SfcI C'TryA_G
Cuts at: 826 1045 2737 3872 4553 4744 826
Size: 219 1692 1135 681 191 1471
Fragments arranged by size:
1692 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5389
SmaI CCC'GGG
Cuts at: 1872 2716 1872
Size: 844 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2113 2365 3493 4394 4656 820
Size: 197 926 170 252 1128 901 262 1553
Fragments arranged by size:
1553 1128 926 901 262 252 197 170
SnaBI TAC'GTA
Cuts at: 493 3180 493
Size: 2687 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5389
SphI G_CATG'C
Cuts at: 1132 2747 1132
Size: 1615 3774
SspI AAT'ATT
Cuts at: 5 52 3313 5
Size: 47 3261 2081
Fragments arranged by size:
3261 2081 47
StuI AGG'CCT
Cuts at: 1517 2282 1517
Size: 765 4624
StyI C'CwwG_G
Cuts at: 513 1065 2004 2828 513
Size: 552 939 824 3074
Fragments arranged by size:
3074 939 824 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3416 3433 3586 3771 4216 325
Size: 1255 1836 17 153 185 445 1498
Fragments arranged by size:
1836 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3635 5347 96
Size: 274 80 33 51 1443 1658 1712 138
Fragments arranged by size:
1712 1658 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 5349 5381 2496
Size: 32 2821 32 2504
Fragments arranged by size:
2821 2504 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5389
XbaI T'CTAG_A
Cuts at: 2329 2725 2329
Size: 396 4993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2392 2392
Size: 5389
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5389
XmaI C'CCGG_G
Cuts at: 1870 2714 1870
Size: 844 4545
XmnI GAAnn'nnTTC
Cuts at: 2346 3518 2346
Size: 1172 4217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2023 3197 276
Size: 53 83 186 936 489 1174 2468
Fragments arranged by size:
2468 1174 936 489 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI