Restriction map for
pFN21AB6373
(Circular) (Six-base) MAPSORT of: pFN21AB6373.seq Check: 1240 from: 1 to: 5515
pFN21AB6373 5515 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 2789 1166
Size: 1623 3892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2747 1184
Size: 1563 3952
AlwNI CAG_nnn'CTG
Cuts at: 2335 2647 3258 4830 2335
Size: 312 611 1572 3020
Fragments arranged by size:
3020 1572 611 312
ApaI G_GGCC'C
Cuts at: 2107 2149 2107
Size: 42 5473
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2840 1870
Size: 73 163 734 4545
Fragments arranged by size:
4545 734 163 73
AvrII C'CTAG_G
Cuts at: 2609 2609
Size: 5515
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2836 4291 618
Size: 325 217 627 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2107 2149 2163 2834 1391
Size: 716 42 14 671 4072
Fragments arranged by size:
4072 716 671 42 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2219 928
Size: 971 320 4224
Fragments arranged by size:
4224 971 320
BbvCI CC'TCA_GC
Cuts at: 2670 2670
Size: 5515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2244 2658 4126
Size: 107 122 71 631 605 572 414 1468
Cuts at: 4126 136
Size: 1525
Fragments arranged by size:
1525 1468 631 605 572 414 122 107
71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2149 3576 4732 1790
Size: 317 42 1427 1156 2573
Fragments arranged by size:
2573 1427 1156 317 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2360 2657 3246
Size: 416 53 83 399 24 943 297 589
Cuts at: 3246 4204 442
Size: 958 1753
Fragments arranged by size:
1753 958 943 589 416 399 297 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2187 2357 2407 4175 1239
Size: 932 16 170 50 1768 2579
Fragments arranged by size:
2579 1768 932 170 50 16
Bpu10I CC'TnA_GC
Cuts at: 2670 2670
Size: 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2222 2300 4178 882
Size: 617 723 78 1878 2219
Fragments arranged by size:
2219 1878 723 617 78
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2796 3322 3704 4339
Size: 53 83 186 936 1263 526 382 635
Cuts at: 4339 275
Size: 1451
Fragments arranged by size:
1451 1263 936 635 526 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 1178
Size: 30 165 30 1131 30 4129
Fragments arranged by size:
4129 1131 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 2597 1186
Size: 1132 279 4104
Fragments arranged by size:
4104 1132 279
BseYI C'CCAG_C
Cuts at: 2072 2640 2755 4718 5214 2072
Size: 568 115 1963 496 2373
Fragments arranged by size:
2373 1963 568 496 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2701 2769 1163
Size: 573 965 68 3909
Fragments arranged by size:
3909 965 573 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 2786 3576 3661 4732 2163
Size: 623 790 85 1071 2946
Fragments arranged by size:
2946 1071 790 623 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2149 2163 2786 2834 3576 3661
Size: 399 317 42 14 623 48 742 85
Cuts at: 3661 4732 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 623 399 317 85 48
42 14
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 2870 844
Size: 1553 473 3489
Fragments arranged by size:
3489 1553 473
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4159 4346 1700
Size: 110 2349 187 2869
Fragments arranged by size:
2869 2349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 2083 1925
Size: 158 5357
BssSI C'ACGA_G
Cuts at: 2735 3575 4587 2735
Size: 840 1012 3663
Fragments arranged by size:
3663 1012 840
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2506 2431
Size: 75 5440
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2301 2301
Size: 5515
BtgI C'CryG_G
Cuts at: 513 1065 2004 2790 513
Size: 552 939 786 3238
Fragments arranged by size:
3238 939 786 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2694 3031 3825 3845 2694
Size: 337 794 20 4364
Fragments arranged by size:
4364 794 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4522 809
Size: 507 3206 1802
Fragments arranged by size:
3206 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 2275 2443
Size: 54 1425 50 275 18 415 30 168
Cuts at: 2443 2469 2962 3851 5480 8
Size: 26 493 889 1629 43
Fragments arranged by size:
1629 1425 889 493 415 275 168 54
50 43 30 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2390 3445 1353
Size: 1037 1055 3423
Fragments arranged by size:
3423 1055 1037
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2225 2315 4102 4476 4622 387
Size: 1064 138 636 90 1787 374 146 1280
Fragments arranged by size:
1787 1280 1064 636 374 146 138 90
EcoICRI GAG'CTC
Cuts at: 2161 2161
Size: 5515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2187 2357 2407 3578 4175 4962
Size: 393 539 16 170 50 1171 597 787
Cuts at: 4962 5230 1239
Size: 268 1524
Fragments arranged by size:
1524 1171 787 597 539 393 268 170
50 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 5515
EcoO109I rG'GnC_Cy
Cuts at: 2103 2146 2103
Size: 43 5472
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
EcoRV GAT'ATC
Cuts at: 2806 2806
Size: 5515
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 4662 1678
Size: 2984 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 2100 2132 4156
Size: 32 135 32 489 32 7 32 2024
Cuts at: 4156 4188 4230 4262 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2024 489 135 42 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 2542 2859 3105 669
Size: 988 281 328 276 317 246 3079
Fragments arranged by size:
3079 988 328 317 281 276 246
HindIII A'AGCT_T
Cuts at: 748 2502 748
Size: 1754 3761
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2759 4629 4813 1305
Size: 1454 1870 184 2007
Fragments arranged by size:
2007 1870 1454 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2277 2471 10
Size: 54 1425 343 445 194 3054
Fragments arranged by size:
3054 1425 445 343 194 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 3473 3832 3991 518
Size: 648 1623 684 359 159 2042
Fragments arranged by size:
2042 1623 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2332 2575 2669 2931 3608 4756 5001
Size: 494 232 243 94 262 677 1148 245
Cuts at: 5001 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 494 262 245 243 232
94
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4024
Fragments arranged by size:
4024 939 552
NdeI CA'TA_TG
Cuts at: 387 2527 387
Size: 2140 3375
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2635 2873 4418 1132
Size: 97 1406 238 1545 2229
Fragments arranged by size:
2229 1545 1406 238 97
PasI CC'CwG_GG
Cuts at: 1348 2317 1348
Size: 969 4546
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 2647 1108
Size: 1227 312 3976
Fragments arranged by size:
3976 1227 312
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2103 2145 2103
Size: 42 5473
PstI C_TGCA'G
Cuts at: 830 2867 830
Size: 2037 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2332 2332
Size: 5515
SacI G_AGCT'C
Cuts at: 2163 2163
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2863 3998 4679 4870 826
Size: 219 1818 1135 681 191 1471
Fragments arranged by size:
1818 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2658 2658
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2635 2873 1132
Size: 1503 238 3774
Fragments arranged by size:
3774 1503 238
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 2299 1517
Size: 782 4733
StyI C'CwwG_G
Cuts at: 513 1065 2004 2475 2609 2954 513
Size: 552 939 471 134 345 3074
Fragments arranged by size:
3074 939 552 471 345 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2298 3542 3559 3712 3897 4342 325
Size: 1255 718 1244 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1244 718 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2562 2562
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI
SrfI SwaI