Restriction map for
pFN21AB6371
(Circular) (Six-base) MAPSORT of: pFN21AB6371.seq Check: 9249 from: 1 to: 5512
pFN21AB6371 5512 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3575 4959 5227 1632
Size: 1943 1384 268 1917
Fragments arranged by size:
1943 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2605 820
Size: 197 1588 3727
Fragments arranged by size:
3727 1588 197
AflIII A'CryG_T
Cuts at: 1203 2809 4411 5388 1203
Size: 1606 1602 977 1327
Fragments arranged by size:
1606 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 2635 3255 4827 2635
Size: 620 1572 3320
Fragments arranged by size:
3320 1572 620
ApaI G_GGCC'C
Cuts at: 2447 2447
Size: 5512
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2208 2555 2821 3047 3199 5308 1493
Size: 715 347 266 226 152 2109 1697
Fragments arranged by size:
2109 1697 715 347 266 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 1870
Size: 73 894 4545
Fragments arranged by size:
4545 894 73
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2833 4288 618
Size: 325 217 627 1046 1455 1842
Fragments arranged by size:
1842 1455 1046 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2066 2447 2512 2831 1391
Size: 675 381 65 319 4072
Fragments arranged by size:
4072 675 381 319 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3703 3737 1903
Size: 34 50 34 1682 34 3678
Fragments arranged by size:
3678 1682 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2070 4123 136
Size: 107 122 71 631 605 398 2053 1525
Fragments arranged by size:
2053 1525 631 605 398 122 107 71
BglII A'GATC_T
Cuts at: 5507 5507
Size: 5512
BlpI GC'TnA_GC
Cuts at: 2012 2929 2012
Size: 917 4595
Bme1580I G_kGCm'C
Cuts at: 1790 2447 3573 4729 1790
Size: 657 1126 1156 2573
Fragments arranged by size:
2573 1156 1126 657
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3243 4201 442
Size: 416 53 83 399 24 1826 958 1753
Fragments arranged by size:
1826 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4172 1239
Size: 2933 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2392 2420 4175 882
Size: 617 893 28 1755 2219
Fragments arranged by size:
2219 1755 893 617 28
BsaAI yAC'GTr
Cuts at: 493 1204 3303 493
Size: 711 2099 2702
Fragments arranged by size:
2702 2099 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3319 3701 4336 275
Size: 53 83 186 936 1786 382 635 1451
Fragments arranged by size:
1786 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3945 4343 4617 4764 5175 3945
Size: 398 274 147 411 4282
Fragments arranged by size:
4282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5287
Fragments arranged by size:
5287 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5512
BseYI C'CCAG_C
Cuts at: 4715 5211 4715
Size: 496 5016
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4939
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3723 4751 1540
Size: 275 186 961 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3573 3658 4729 3573
Size: 85 1071 4356
Fragments arranged by size:
4356 1071 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
BsmBI CGTCTCn'nnnn_
Cuts at: 2606 2606
Size: 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2447 2512 2831 3573 3658 4729
Size: 399 276 381 65 319 742 85 1071
Cuts at: 4729 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 381 319 276 85
65
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2776 2788 2867 844
Size: 1932 12 79 3489
Fragments arranged by size:
3489 1932 79 12
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2103 4007 4181 65
Size: 1183 855 1904 174 1396
Fragments arranged by size:
1904 1396 1183 855 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4156 4343 1700
Size: 110 2346 187 2869
Fragments arranged by size:
2869 2346 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5512
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2759 794
Size: 1965 3547
BstBI TT'CG_AA
Cuts at: 2029 4316 2029
Size: 2287 3225
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2842 3209 3594 3611 5063 5149 5161 5507
Size: 870 367 385 17 1452 86 12 346
Cuts at: 5507 1972
Size: 1977
Fragments arranged by size:
1977 1452 870 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4960
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 2327 3028 3822 3842 2327
Size: 701 794 20 3997
Fragments arranged by size:
3997 794 701 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2574 2816 2574
Size: 242 5270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2048 4519 809
Size: 507 732 2471 1802
Fragments arranged by size:
2471 1802 732 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2392 2959 3848
Size: 54 1425 50 275 18 562 567 889
Cuts at: 3848 5477 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 567 562 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2959 1537
Size: 275 1147 4090
Fragments arranged by size:
4090 1147 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3442 1353
Size: 2089 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4099 4473 4619 387
Size: 1064 138 2510 374 146 1280
Fragments arranged by size:
2510 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3575 4172 4959 5227 1239
Size: 393 1943 597 787 268 1524
Fragments arranged by size:
1943 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2444 2632 2444
Size: 188 5324
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 4659 1678
Size: 2981 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2693 2725 4153 4185 4227
Size: 32 135 32 1121 32 1428 32 42
Cuts at: 4227 4259 1373
Size: 32 2626
Fragments arranged by size:
2626 1428 1121 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2221 2856 3102 669
Size: 988 281 283 635 246 3079
Fragments arranged by size:
3079 988 635 283 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 2241 3111 2241
Size: 870 4642
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4626 4810 1305
Size: 3321 184 2007
Fragments arranged by size:
3321 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3690
Fragments arranged by size:
3690 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3470 3829 3988 518
Size: 648 2304 359 159 2042
Fragments arranged by size:
2304 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2928 3605 4753 4998 1606
Size: 1322 677 1148 245 2120
Fragments arranged by size:
2120 1322 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4960
NdeI CA'TA_TG
Cuts at: 387 2404 2547 387
Size: 2017 143 3352
Fragments arranged by size:
3352 2017 143
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2813 2870 4415 1132
Size: 97 1584 57 1545 2229
Fragments arranged by size:
2229 1584 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 2068 1348
Size: 720 4792
PciI A'CATG_T
Cuts at: 2809 4411 2809
Size: 1602 3910
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PfoI T'CCnGG_A
Cuts at: 2718 2718
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 5512
PshAI GACnn'nnGTC
Cuts at: 1613 2548 1613
Size: 935 4577
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PspOMI G'GGCC_C
Cuts at: 2443 2443
Size: 5512
PstI C_TGCA'G
Cuts at: 830 2492 2626 2785 2864 830
Size: 1662 134 159 79 3478
Fragments arranged by size:
3478 1662 159 134 79
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2488 2622 2781 2860 3995 4676 4867
Size: 219 1443 134 159 79 1135 681 191
Cuts at: 4867 826
Size: 1471
Fragments arranged by size:
1471 1443 1135 681 219 191 159 134
79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2605 3616 4517 4779 820
Size: 197 926 662 1011 901 262 1553
Fragments arranged by size:
1553 1011 926 901 662 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 2581 152
Size: 2429 3083
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5512
StyI C'CwwG_G
Cuts at: 513 1065 2951 513
Size: 552 1886 3074
Fragments arranged by size:
3074 1886 552
SwaI ATTT'AAAT
Cuts at: 2574 2574
Size: 5512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3556 3709 3894 4339 325
Size: 1255 1959 17 153 185 445 1498
Fragments arranged by size:
1959 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3758 5470 96
Size: 274 80 33 51 1443 1781 1712 138
Fragments arranged by size:
1781 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5472 5504 5472
Size: 32 5480
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5512
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI XcmI