Restriction map for
pFN21AB6370
(Circular) (Six-base) MAPSORT of: pFN21AB6370.seq Check: 5979 from: 1 to: 5599
pFN21AB6370 5599 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2136 3409 278
Size: 53 83 186 936 600 1273 2468
Fragments arranged by size:
2468 1273 936 600 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2523 2942 1937
Size: 586 419 4594
Fragments arranged by size:
4594 586 419
Acc65I G'GTAC_C
Cuts at: 2920 2920
Size: 5599
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 2693 3662 5046 5314 1632
Size: 623 438 969 1384 268 1917
Fragments arranged by size:
1917 1384 969 623 438 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 4498 5475 1203
Size: 3295 977 1327
Fragments arranged by size:
3295 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 2203 4328 2203
Size: 2125 3474
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5599
AlwNI CAG_nnn'CTG
Cuts at: 3342 4914 3342
Size: 1572 4027
ApaLI G'TGCA_C
Cuts at: 2714 3656 4812 2714
Size: 942 1156 3501
Fragments arranged by size:
3501 1156 942
ApoI r'AATT_y
Cuts at: 1493 2441 2908 3134 3286 5395 1493
Size: 948 467 226 152 2109 1697
Fragments arranged by size:
2109 1697 948 467 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2724 2924 1870
Size: 73 781 200 4545
Fragments arranged by size:
4545 781 200 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2176 2209 2176
Size: 33 5566
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2881 2920 4375 618
Size: 325 217 627 230 864 39 1455 1842
Fragments arranged by size:
1842 1455 864 627 325 230 217 39
BanII G_rGCy'C
Cuts at: 1391 2918 1391
Size: 1527 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 928
Size: 971 237 4391
Fragments arranged by size:
4391 971 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2705 2739 3790 3824 1903
Size: 34 768 34 1051 34 3678
Fragments arranged by size:
3678 1051 768 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3491 4701 3491
Size: 1210 4389
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4210 136
Size: 107 122 71 631 605 2538 1525
Fragments arranged by size:
2538 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5594 5594
Size: 5599
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 2564 2718 2817 2886 3660 4816 1790
Size: 774 154 99 69 774 1156 2573
Fragments arranged by size:
2573 1156 774 774 154 99 69
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5599
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2118 3330 4288
Size: 416 53 83 399 24 701 1212 958
Cuts at: 4288 442
Size: 1753
Fragments arranged by size:
1753 1212 958 701 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2245 2570 4259 1239
Size: 917 89 325 1689 2579
Fragments arranged by size:
2579 1689 917 325 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 4589 4887 3724
Size: 865 298 4436
Fragments arranged by size:
4436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 2349 4262 882
Size: 617 795 55 1913 2219
Fragments arranged by size:
2219 1913 795 617 55
BsaAI yAC'GTr
Cuts at: 493 1204 2350 2527 2848 3390 493
Size: 711 1146 177 321 542 2702
Fragments arranged by size:
2702 1146 711 542 321 177
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 3406 3788 4423
Size: 53 83 186 936 600 1273 382 635
Cuts at: 4423 275
Size: 1451
Fragments arranged by size:
1451 1273 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4032 4430 4704 4851 5262 4032
Size: 398 274 147 411 4369
Fragments arranged by size:
4369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 2735 2765 1178
Size: 30 165 30 1140 30 162 30 4012
Fragments arranged by size:
4012 1140 165 162 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2567 2570 1186
Size: 1381 3 4215
Fragments arranged by size:
4215 1381 3
BseYI C'CCAG_C
Cuts at: 2458 2479 2868 4802 5298 2458
Size: 21 389 1934 496 2759
Fragments arranged by size:
2759 1934 496 389 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2549 2759 2870 1163
Size: 573 813 210 111 3892
Fragments arranged by size:
3892 813 573 210 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3049 3810 4838 1540
Size: 275 186 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2718 3660 3745 4816 2718
Size: 942 85 1071 3501
Fragments arranged by size:
3501 1071 942 85
BsmI GAATG_Cn'
Cuts at: 2402 3110 3203 2402
Size: 708 93 4798
Fragments arranged by size:
4798 708 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2564 2718 2817 2886 2918 3660 3745
Size: 399 774 154 99 69 32 742 85
Cuts at: 3745 4816 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 774 742 399 154 99 85
69 32
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2954 844
Size: 2110 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3486 1394
Size: 429 1663 3507
Fragments arranged by size:
3507 1663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2610 2729 4243 4430 1700
Size: 110 800 119 1514 187 2869
Fragments arranged by size:
2869 1514 800 187 119 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5599
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 2876 3659 4671 2876
Size: 783 1012 3804
Fragments arranged by size:
3804 1012 783
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5599
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 2635 1184
Size: 1451 4148
BstYI r'GATC_y
Cuts at: 1972 2617 2929 3296 3681 3698 5150 5236 5248
Size: 645 312 367 385 17 1452 86 12
Cuts at: 5248 5594 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 645 385 367 346 312 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2214 513
Size: 552 939 210 3898
Fragments arranged by size:
3898 939 552 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4860
Fragments arranged by size:
4860 683 56
BtsI GCAGTG_nn'
Cuts at: 2817 3115 3909 3929 2817
Size: 298 794 20 4487
Fragments arranged by size:
4487 794 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4606 809
Size: 507 3290 1802
Fragments arranged by size:
3290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3046 3935 5564
Size: 54 1425 50 275 18 1216 889 1629
Cuts at: 5564 8
Size: 43
Fragments arranged by size:
1629 1425 1216 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3046 1537
Size: 275 1234 4090
Fragments arranged by size:
4090 1234 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3529 1353
Size: 2176 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4186 4560 4706 387
Size: 1064 138 2597 374 146 1280
Fragments arranged by size:
2597 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2245 2255 2570 2693 3662 4259
Size: 393 524 89 10 315 123 969 597
Cuts at: 4259 5046 5314 1239
Size: 787 268 1524
Fragments arranged by size:
1524 969 787 597 524 393 315 268
123 89 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 2552
Size: 5599
EcoO109I rG'GnC_Cy
Cuts at: 2051 2149 2467 2650 2051
Size: 98 318 183 5000
Fragments arranged by size:
5000 318 183 98
EcoRI G'AATT_C
Cuts at: 2441 2908 3286 2441
Size: 467 378 4754
Fragments arranged by size:
4754 467 378
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 4746 1678
Size: 3068 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 2609 2641 4240
Size: 32 135 32 617 32 388 32 1599
Cuts at: 4240 4272 4314 4346 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1599 617 388 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2943 3189 669
Size: 988 281 1005 246 3079
Fragments arranged by size:
3079 1005 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KpnI G_GTAC'C
Cuts at: 2924 2924
Size: 5599
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 4897 1305
Size: 3408 184 2007
Fragments arranged by size:
3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3777
Fragments arranged by size:
3777 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 3557 3916 4075 518
Size: 648 1128 1263 359 159 2042
Fragments arranged by size:
2042 1263 1128 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2397 2533 3015 3692 4840 5085 1606
Size: 791 136 482 677 1148 245 2120
Fragments arranged by size:
2120 1148 791 677 482 245 136
NaeI GCC'GGC
Cuts at: 2731 2731
Size: 5599
NcoI C'CATG_G
Cuts at: 513 1065 2004 2214 513
Size: 552 939 210 3898
Fragments arranged by size:
3898 939 552 210
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NgoMIV G'CCGG_C
Cuts at: 2729 2729
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2126 2957 4502 1132
Size: 97 897 831 1545 2229
Fragments arranged by size:
2229 1545 897 831 97
PasI CC'CwG_GG
Cuts at: 1348 2699 2886 1348
Size: 1351 187 4061
Fragments arranged by size:
4061 1351 187
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5599
PfoI T'CCnGG_A
Cuts at: 2604 2604
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PmlI CAC'GTG
Cuts at: 2848 2848
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2051 2467 2051
Size: 416 5183
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2951 830
Size: 2121 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SanDI GG'GwC_CC
Cuts at: 2467 2467
Size: 5599
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SfcI C'TryA_G
Cuts at: 826 1045 2947 4082 4763 4954 826
Size: 219 1902 1135 681 191 1471
Fragments arranged by size:
1902 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3703 4604 4866 820
Size: 197 926 1760 901 262 1553
Fragments arranged by size:
1760 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2957 1132
Size: 1825 3774
SspI AAT'ATT
Cuts at: 5 52 2414 3523 5
Size: 47 2362 1109 2081
Fragments arranged by size:
2362 2081 1109 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5599
StyI C'CwwG_G
Cuts at: 513 1065 2004 2152 2214 2538 3038 513
Size: 552 939 148 62 324 500 3074
Fragments arranged by size:
3074 939 552 500 324 148 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2788 3626 3643 3796 3981 4426 325
Size: 1255 1208 838 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1208 838 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3845 5557 96
Size: 274 80 33 51 1443 1868 1712 138
Fragments arranged by size:
1868 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 5559 5591 2543
Size: 32 2984 32 2551
Fragments arranged by size:
2984 2551 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2545 2011
Size: 534 5065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2134 3407 276
Size: 53 83 186 936 600 1273 2468
Fragments arranged by size:
2468 1273 936 600 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI