Restriction map for
pFN21AB6366
(Circular) (Six-base) MAPSORT of: pFN21AB6366.seq Check: 1427 from: 1 to: 5599
pFN21AB6366 5599 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2397 2942 1937
Size: 460 545 4594
Fragments arranged by size:
4594 545 460
Acc65I G'GTAC_C
Cuts at: 2920 2920
Size: 5599
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3662 5046 5314 1632
Size: 2030 1384 268 1917
Fragments arranged by size:
2030 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 4498 5475 1203
Size: 3295 977 1327
Fragments arranged by size:
3295 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5599
AlwNI CAG_nnn'CTG
Cuts at: 3342 4914 3342
Size: 1572 4027
ApaLI G'TGCA_C
Cuts at: 2627 3656 4812 2627
Size: 1029 1156 3414
Fragments arranged by size:
3414 1156 1029
ApoI r'AATT_y
Cuts at: 1493 2908 3134 3286 5395 1493
Size: 1415 226 152 2109 1697
Fragments arranged by size:
2109 1697 1415 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2447 2822 2920 4375 618
Size: 325 217 627 660 375 98 1455 1842
Fragments arranged by size:
1842 1455 660 627 375 325 217 98
BanII G_rGCy'C
Cuts at: 1391 2523 2918 1391
Size: 1132 395 4072
Fragments arranged by size:
4072 1132 395
BbeI G_GCGC'C
Cuts at: 2451 2451
Size: 5599
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3790 3824 1903
Size: 34 1853 34 3678
Fragments arranged by size:
3678 1853 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3491 4701 3491
Size: 1210 4389
BclI T'GATC_A
Cuts at: 1548 2011 1548
Size: 463 5136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2375 4210 136
Size: 107 122 71 631 605 703 1835 1525
Fragments arranged by size:
1835 1525 703 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2493 5594 2493
Size: 3101 2498
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 2631 3660 4816 1790
Size: 841 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 841
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5599
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3330 4288 442
Size: 416 53 83 399 24 1913 958 1753
Fragments arranged by size:
1913 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2797 4259 1239
Size: 997 561 1462 2579
Fragments arranged by size:
2579 1462 997 561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 4589 4887 3724
Size: 865 298 4436
Fragments arranged by size:
4436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3390 493
Size: 711 2186 2702
Fragments arranged by size:
2702 2186 711
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2357 2448 3406
Size: 53 83 186 936 519 305 91 958
Cuts at: 3406 3788 4423 275
Size: 382 635 1451
Fragments arranged by size:
1451 958 936 635 519 382 305 186
91 83 53
BsaWI w'CCGG_w
Cuts at: 2360 4032 4430 4704 4851 5262 2360
Size: 1672 398 274 147 411 2697
Fragments arranged by size:
2697 1672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5374
Fragments arranged by size:
5374 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5599
BseYI C'CCAG_C
Cuts at: 2372 2454 4802 5298 2372
Size: 82 2348 496 2673
Fragments arranged by size:
2673 2348 496 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5026
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 2734 3049 3810 4838 1540
Size: 275 186 81 652 315 761 1028 2301
Fragments arranged by size:
2301 1028 761 652 315 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 2631 3660 3745 4816 2631
Size: 1029 85 1071 3414
Fragments arranged by size:
3414 1071 1029 85
BsmI GAATG_Cn'
Cuts at: 3110 3203 3110
Size: 93 5506
BsmBI CGTCTCn'nnnn_
Cuts at: 2071 2545 2071
Size: 474 5125
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2631 2918 3660 3745 4816 1391
Size: 399 733 108 287 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 399 287 108 85
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2104 2954 844
Size: 1260 850 3489
Fragments arranged by size:
3489 1260 850
BsrBI CCG'CTC
Cuts at: 1394 1823 3486 1394
Size: 429 1663 3507
Fragments arranged by size:
3507 1663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4243 4430 1700
Size: 110 2433 187 2869
Fragments arranged by size:
2869 2433 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 2941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 3659 4671 3659
Size: 1012 4587
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5599
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 2222 2348 1184
Size: 1038 126 4435
Fragments arranged by size:
4435 1038 126
BstYI r'GATC_y
Cuts at: 1972 2493 2929 3296 3681 3698 5150 5236 5248
Size: 521 436 367 385 17 1452 86 12
Cuts at: 5248 5594 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 521 436 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2190 2513 2190
Size: 323 5276
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 2467 513
Size: 552 939 72 391 3645
Fragments arranged by size:
3645 939 552 391 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4860
Fragments arranged by size:
4860 683 56
BtsI GCAGTG_nn'
Cuts at: 3115 3909 3929 3115
Size: 794 20 4785
Fragments arranged by size:
4785 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4606 809
Size: 507 3290 1802
Fragments arranged by size:
3290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2229 2731
Size: 54 1425 50 275 18 213 186 502
Cuts at: 2731 3046 3935 5564 8
Size: 315 889 1629 43
Fragments arranged by size:
1629 1425 889 502 315 275 213 186
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2731 3046 1537
Size: 275 919 315 4090
Fragments arranged by size:
4090 919 315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2303 3529 1353
Size: 950 1226 3423
Fragments arranged by size:
3423 1226 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4186 4560 4706 387
Size: 1064 138 2597 374 146 1280
Fragments arranged by size:
2597 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2797 3662 4259 5046 5314 1239
Size: 393 604 561 865 597 787 268 1524
Fragments arranged by size:
1524 865 787 604 597 561 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2215 2215
Size: 5599
EcoRI G'AATT_C
Cuts at: 2908 3286 2908
Size: 378 5221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2830 2314
Size: 516 5083
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 2338 2451 4746 1678
Size: 660 113 2295 2531
Fragments arranged by size:
2531 2295 660 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4240 4272 4314 4346 1373
Size: 32 135 32 2668 32 42 32 2626
Fragments arranged by size:
2668 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2770 2943 3189 669
Size: 988 281 832 173 246 3079
Fragments arranged by size:
3079 988 832 281 246 173
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KasI G'GCGC_C
Cuts at: 2447 2447
Size: 5599
KpnI G_GTAC'C
Cuts at: 2924 2924
Size: 5599
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 4897 1305
Size: 3408 184 2007
Fragments arranged by size:
3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2231 10
Size: 54 1425 343 399 3378
Fragments arranged by size:
3378 1425 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 3557 3916 4075 518
Size: 648 1470 921 359 159 2042
Fragments arranged by size:
2042 1470 921 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2078 2372 2404 3015 3692 4840 5085
Size: 454 18 294 32 611 677 1148 245
Cuts at: 5085 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 611 454 294 245 32
18
NarI GG'CG_CC
Cuts at: 2448 2448
Size: 5599
NcoI C'CATG_G
Cuts at: 513 1065 2004 2467 513
Size: 552 939 463 3645
Fragments arranged by size:
3645 939 552 463
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2126 2957 4502 1132
Size: 97 897 831 1545 2229
Fragments arranged by size:
2229 1545 897 831 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2473 1108
Size: 1365 4234
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2078 2669 1613
Size: 465 591 4543
Fragments arranged by size:
4543 591 465
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2115 2951 830
Size: 1285 836 3478
Fragments arranged by size:
3478 1285 836
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
SacII CC_GC'GG
Cuts at: 2079 2079
Size: 5599
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SapI GCTCTTCn'nnn_
Cuts at: 2303 2303
Size: 5599
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SexAI A'CCwGG_T
Cuts at: 2420 2420
Size: 5599
SfcI C'TryA_G
Cuts at: 826 1045 2111 2563 2947 4082 4763 4954 826
Size: 219 1066 452 384 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 681 452 384 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2375 2375
Size: 5599
SfoI GGC'GCC
Cuts at: 2449 2449
Size: 5599
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3703 4604 4866 820
Size: 197 926 1760 901 262 1553
Fragments arranged by size:
1760 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2126 2957 1132
Size: 994 831 3774
Fragments arranged by size:
3774 994 831
SspI AAT'ATT
Cuts at: 5 52 3523 5
Size: 47 3471 2081
Fragments arranged by size:
3471 2081 47
StuI AGG'CCT
Cuts at: 1517 2810 1517
Size: 1293 4306
StyI C'CwwG_G
Cuts at: 513 1065 2004 2467 2548 2598 2805 3038 513
Size: 552 939 463 81 50 207 233 3074
Fragments arranged by size:
3074 939 552 463 233 207 81 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2270 3626 3643 3796 3981 4426
Size: 1255 417 273 1356 17 153 185 445
Cuts at: 4426 325
Size: 1498
Fragments arranged by size:
1498 1356 1255 445 417 273 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 2286 2754
Size: 274 80 33 51 1443 177 132 468
Cuts at: 2754 3845 5557 96
Size: 1091 1712 138
Fragments arranged by size:
1712 1443 1091 468 274 177 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5559 5591 5559
Size: 32 5567
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SanDI SrfI SwaI XcmI