Restriction map for
pFN21AB6362
(Circular) (Six-base) MAPSORT of: pFN21AB6362.seq Check: 9132 from: 1 to: 5578
pFN21AB6362 5578 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2921 1937
Size: 984 4594
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2696 3641 5025 5293 1632
Size: 1064 945 1384 268 1917
Fragments arranged by size:
1917 1384 1064 945 268
AfeI AGC'GCT
Cuts at: 2264 2558 2264
Size: 294 5284
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 2575 4307 2575
Size: 1732 3846
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 5546
AlwNI CAG_nnn'CTG
Cuts at: 2323 3321 4893 2323
Size: 998 1572 3008
Fragments arranged by size:
3008 1572 998
ApaLI G'TGCA_C
Cuts at: 2142 2852 3635 4791 2142
Size: 710 783 1156 2929
Fragments arranged by size:
2929 1156 783 710
ApoI r'AATT_y
Cuts at: 1493 2887 3113 3265 5374 1493
Size: 1394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2903 1870
Size: 73 152 808 4545
Fragments arranged by size:
4545 808 152 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2899 4354 618
Size: 325 217 627 1112 1455 1842
Fragments arranged by size:
1842 1455 1112 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2343 2459 2897 1391
Size: 705 247 116 438 4072
Fragments arranged by size:
4072 705 438 247 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2436 2664 928
Size: 971 537 228 3842
Fragments arranged by size:
3842 971 537 228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2123 2157 3769 3803 1903
Size: 34 50 34 102 34 1612 34 3678
Fragments arranged by size:
3678 1612 102 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4189 136
Size: 107 122 71 631 605 2517 1525
Fragments arranged by size:
2517 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 2146 2856 3639 4795 1790
Size: 356 710 783 1156 2573
Fragments arranged by size:
2573 1156 783 710 356
BmgBI CAC'GTC
Cuts at: 1318 2220 2851 1318
Size: 902 631 4045
Fragments arranged by size:
4045 902 631
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2146 3309 4267
Size: 416 53 83 399 24 729 1163 958
Cuts at: 4267 442
Size: 1753
Fragments arranged by size:
1753 1163 958 729 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 2452 4238 1239
Size: 1149 64 1786 2579
Fragments arranged by size:
2579 1786 1149 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3703 4568 4866 3703
Size: 865 298 4415
Fragments arranged by size:
4415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3369 493
Size: 711 2165 2702
Fragments arranged by size:
2702 2165 711
BsaBI GATnn'nnATC
Cuts at: 2522 5572 2522
Size: 3050 2528
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3385 3767 4402 275
Size: 53 83 186 936 1852 382 635 1451
Fragments arranged by size:
1852 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4011 4409 4683 4830 5241 4011
Size: 398 274 147 411 4348
Fragments arranged by size:
4348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 2591 2621 1178
Size: 30 165 30 1041 30 117 30 4135
Fragments arranged by size:
4135 1041 165 117 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2596 1186
Size: 1410 4168
BseYI C'CCAG_C
Cuts at: 4781 5277 4781
Size: 496 5082
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5005
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2312 2335 3028 3789 4817 1540
Size: 275 186 311 23 693 761 1028 2301
Fragments arranged by size:
2301 1028 761 693 311 275 186 23
BsiHKAI G_wGCw'C
Cuts at: 2096 2146 2588 2856 3639 3724 4795 2096
Size: 50 442 268 783 85 1071 2879
Fragments arranged by size:
2879 1071 783 442 268 85 50
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
BsmBI CGTCTCn'nnnn_
Cuts at: 2470 2470
Size: 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2146 2343 2459 2588 2856 2897
Size: 399 306 50 197 116 129 268 41
Cuts at: 2897 3639 3724 4795 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 268 197 129
116 85 50 41
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2933 844
Size: 1547 542 3489
Fragments arranged by size:
3489 1547 542
BsrBI CCG'CTC
Cuts at: 1394 1823 3465 1394
Size: 429 1642 3507
Fragments arranged by size:
3507 1642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4073 4247 65
Size: 1183 2825 174 1396
Fragments arranged by size:
2825 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2071 2441 4222 4409 1700
Size: 110 261 370 1781 187 2869
Fragments arranged by size:
2869 1781 370 261 187 110
BsrGI T'GTAC_A
Cuts at: 96 2707 96
Size: 2611 2967
BssHII G'CGCG_C
Cuts at: 1925 2157 2374 1925
Size: 232 217 5129
Fragments arranged by size:
5129 232 217
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 794
Size: 1529 4049
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2908 3275 3660 3677 5129 5215 5227 5573
Size: 936 367 385 17 1452 86 12 346
Cuts at: 5573 1972
Size: 1977
Fragments arranged by size:
1977 1452 936 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2062 2112 2335 513
Size: 552 997 50 223 3756
Fragments arranged by size:
3756 997 552 223 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4839
Fragments arranged by size:
4839 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 2759 2854 3094 3888 3908 2452
Size: 307 95 240 794 20 4122
Fragments arranged by size:
4122 794 307 240 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DraIII CAC_nnn'GTG
Cuts at: 2482 2482
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2332 3025 3914
Size: 54 1425 50 275 18 502 693 889
Cuts at: 3914 5543 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 693 502 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2332 3025 1537
Size: 275 520 693 4090
Fragments arranged by size:
4090 693 520 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3508 1353
Size: 2155 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2154 4165 4539 4685 387
Size: 1064 138 565 2011 374 146 1280
Fragments arranged by size:
2011 1280 1064 565 374 146 138
EcoICRI GAG'CTC
Cuts at: 2094 2094
Size: 5578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2452 2696 3641 4238 5025 5293
Size: 393 756 64 244 945 597 787 268
Cuts at: 5293 1239
Size: 1524
Fragments arranged by size:
1524 945 787 756 597 393 268 244
64
EcoNI CCTnn'n_nnAGG
Cuts at: 2766 2820 2766
Size: 54 5524
EcoO109I rG'GnC_Cy
Cuts at: 2108 2108
Size: 5578
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 5578
FspI TGC'GCA
Cuts at: 2224 4084 2224
Size: 1860 3718
FspAI rTGC'GCAy
Cuts at: 2224 2224
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2266 2324 2396 2442 2560 4725 1678
Size: 588 58 72 46 118 2165 2531
Fragments arranged by size:
2531 2165 588 118 72 58 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 2444 2476 2561
Size: 32 135 32 438 32 402 32 85
Cuts at: 2561 2593 4219 4251 4293 4325 1373
Size: 32 1626 32 42 32 2626
Fragments arranged by size:
2626 1626 438 402 135 85 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 2569 2922 3168 669
Size: 988 281 412 219 353 246 3079
Fragments arranged by size:
3079 988 412 353 281 246 219
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2403 4692 4876 1305
Size: 1098 2289 184 2007
Fragments arranged by size:
2289 2007 1098 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3536 3895 4054 518
Size: 648 2370 359 159 2042
Fragments arranged by size:
2370 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2036 2064 2314 2337 2994 3671 4819
Size: 405 25 28 250 23 657 677 1148
Cuts at: 4819 5064 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 657 405 250 245 28
25 23
NaeI GCC'GGC
Cuts at: 2443 2443
Size: 5578
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5026
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NgoMIV G'CCGG_C
Cuts at: 2441 2441
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2543 2869 2936 4481 1132
Size: 97 1314 326 67 1545 2229
Fragments arranged by size:
2229 1545 1314 326 97 67
PasI CC'CwG_GG
Cuts at: 1348 2700 1348
Size: 1352 4226
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 1108
Size: 920 4658
PfoI T'CCnGG_A
Cuts at: 2404 2404
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2591 2623 3647 3679 5160 5192 2591
Size: 32 1024 32 1481 32 2977
Fragments arranged by size:
2977 1481 1024 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2108 2108
Size: 5578
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 2742 3148 2742
Size: 406 5172
PstI C_TGCA'G
Cuts at: 830 2319 2930 830
Size: 1489 611 3478
Fragments arranged by size:
3478 1489 611
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
SacI G_AGCT'C
Cuts at: 2096 2096
Size: 5578
SacII CC_GC'GG
Cuts at: 2065 2338 2065
Size: 273 5305
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SanDI GG'GwC_CC
Cuts at: 2108 2108
Size: 5578
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SfcI C'TryA_G
Cuts at: 826 1045 2315 2860 2926 4061 4742 4933 826
Size: 219 1270 545 66 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 545 219 191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 2071 1700
Size: 371 5207
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3682 4583 4845 820
Size: 197 926 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2543 2869 2936 1132
Size: 1411 326 67 3774
Fragments arranged by size:
3774 1411 326 67
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 2835 3017 513
Size: 552 1770 182 3074
Fragments arranged by size:
3074 1770 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3605 3622 3775 3960 4405 325
Size: 1255 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2707 3824 5536
Size: 274 80 33 51 1443 730 1117 1712
Cuts at: 5536 96
Size: 138
Fragments arranged by size:
1712 1443 1117 730 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5538 5570 5538
Size: 32 5546
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SacII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SapI SexAI SfiI SfoI SrfI
SwaI XcmI