Restriction map for
pFN21AB6361
(Circular) (Six-base) MAPSORT of: pFN21AB6361.seq Check: 5914 from: 1 to: 5212
pFN21AB6361 5212 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3022 278
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2555 1937
Size: 618 4594
Acc65I G'GTAC_C
Cuts at: 2533 2533
Size: 5212
AclI AA'CG_TT
Cuts at: 1563 3339 3712 1563
Size: 1776 373 3063
Fragments arranged by size:
3063 1776 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3275 4659 4927 1632
Size: 1643 1384 268 1917
Fragments arranged by size:
1917 1643 1384 268
AfeI AGC'GCT
Cuts at: 2368 2368
Size: 5212
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5015
AflIII A'CryG_T
Cuts at: 1203 4111 5088 1203
Size: 2908 977 1327
Fragments arranged by size:
2908 1327 977
AgeI A'CCGG_T
Cuts at: 4043 4043
Size: 5212
AhdI GACnn_n'nnGTC
Cuts at: 2040 3941 2040
Size: 1901 3311
AleI CACnn'nnGTG
Cuts at: 1166 2324 1166
Size: 1158 4054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5212
AlwNI CAG_nnn'CTG
Cuts at: 2955 4527 2955
Size: 1572 3640
ApaLI G'TGCA_C
Cuts at: 3269 4425 3269
Size: 1156 4056
ApoI r'AATT_y
Cuts at: 1493 2521 2747 2899 5008 1493
Size: 1028 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3766 160
Size: 872 2734 1606
Fragments arranged by size:
2734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
AvaI C'yCGr_G
Cuts at: 1870 1943 2152 2537 1870
Size: 73 209 385 4545
Fragments arranged by size:
4545 385 209 73
BamHI G'GATC_C
Cuts at: 2542 2909 2542
Size: 367 4845
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 2533 3988 618
Size: 325 217 627 441 305 1455 1842
Fragments arranged by size:
1842 1455 627 441 325 305 217
BanII G_rGCy'C
Cuts at: 1391 2097 2159 2531 1391
Size: 706 62 372 4072
Fragments arranged by size:
4072 706 372 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2490 928
Size: 971 591 3650
Fragments arranged by size:
3650 971 591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3403 3437 1903
Size: 34 1466 34 3678
Fragments arranged by size:
3678 1466 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3104 4314 2310
Size: 794 1210 3208
Fragments arranged by size:
3208 1210 794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2444 3823 136
Size: 107 122 71 631 605 772 1379 1525
Fragments arranged by size:
1525 1379 772 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5207 5207
Size: 5212
BlpI GC'TnA_GC
Cuts at: 2363 2629 2363
Size: 266 4946
Bme1580I G_kGCm'C
Cuts at: 1790 3273 4429 1790
Size: 1483 1156 2573
Fragments arranged by size:
2573 1483 1156
BmgBI CAC'GTC
Cuts at: 1318 2340 1318
Size: 1022 4190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2943 3901 442
Size: 416 53 83 399 24 1526 958 1753
Fragments arranged by size:
1753 1526 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3872 1239
Size: 2633 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 3337 4202 4500 2378
Size: 959 865 298 3090
Fragments arranged by size:
3090 959 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2048 3875 882
Size: 617 549 1827 2219
Fragments arranged by size:
2219 1827 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 3003 493
Size: 711 1799 2702
Fragments arranged by size:
2702 1799 711
BsaBI GATnn'nnATC
Cuts at: 5206 5206
Size: 5212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3019 3401 4036 275
Size: 53 83 186 936 1486 382 635 1451
Fragments arranged by size:
1486 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3645 4043 4317 4464 4875 3645
Size: 398 274 147 411 3982
Fragments arranged by size:
3982 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4987
Fragments arranged by size:
4987 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5212
BseYI C'CCAG_C
Cuts at: 2111 2160 4415 4911 2111
Size: 49 2255 496 2412
Fragments arranged by size:
2412 2255 496 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4639
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 2662 3423 4451 1540
Size: 275 186 77 584 761 1028 2301
Fragments arranged by size:
2301 1028 761 584 275 186 77
BsiHKAI G_wGCw'C
Cuts at: 3273 3358 4429 3273
Size: 85 1071 4056
Fragments arranged by size:
4056 1071 85
BsmI GAATG_Cn'
Cuts at: 2723 2816 2723
Size: 93 5119
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2159 2531 3273 3358 4429 1391
Size: 399 307 62 372 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 307 85 62
BspEI T'CCGG_A
Cuts at: 4875 4875
Size: 5212
BspHI T'CATG_A
Cuts at: 2212 3101 4831 2212
Size: 889 1730 2593
Fragments arranged by size:
2593 1730 889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2567 844
Size: 1723 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3099 1394
Size: 429 1276 3507
Fragments arranged by size:
3507 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3707 3881 65
Size: 1183 2459 174 1396
Fragments arranged by size:
2459 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 2452 3856 4043 1700
Size: 110 270 372 1404 187 2869
Fragments arranged by size:
2869 1404 372 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5212
BssHII G'CGCG_C
Cuts at: 1925 2021 1925
Size: 96 5116
BssSI C'ACGA_G
Cuts at: 3272 4284 3272
Size: 1012 4200
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5212
BstBI TT'CG_AA
Cuts at: 4016 4016
Size: 5212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5212
BstYI r'GATC_y
Cuts at: 1972 2542 2909 3294 3311 4763 4849 4861 5207
Size: 570 367 385 17 1452 86 12 346
Cuts at: 5207 1972
Size: 1977
Fragments arranged by size:
1977 1452 570 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2224 2439 513
Size: 552 939 8 212 215 3286
Fragments arranged by size:
3286 939 552 215 212 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4473
Fragments arranged by size:
4473 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 3522 3542 2728
Size: 794 20 4398
Fragments arranged by size:
4398 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5177
DraI TTT'AAA
Cuts at: 2516 2516
Size: 5212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4219 809
Size: 507 2903 1802
Fragments arranged by size:
2903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 2130 2418
Size: 54 1425 50 275 18 245 55 288
Cuts at: 2418 2659 3548 5177 8
Size: 241 889 1629 43
Fragments arranged by size:
1629 1425 889 288 275 245 241 55
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2075 2659 1537
Size: 275 263 584 4090
Fragments arranged by size:
4090 584 275 263
EarI CTCTTCn'nnn_
Cuts at: 1353 3142 1353
Size: 1789 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2263 3799 4173 4319 387
Size: 1064 138 674 1536 374 146 1280
Fragments arranged by size:
1536 1280 1064 674 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3275 3872 4659 4927 1239
Size: 393 1643 597 787 268 1524
Fragments arranged by size:
1643 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2187 2187
Size: 5212
EcoRI G'AATT_C
Cuts at: 2521 2899 2521
Size: 378 4834
FspI TGC'GCA
Cuts at: 3718 3718
Size: 5212
HaeII r_GCGC'y
Cuts at: 1678 2054 2370 4359 1678
Size: 376 316 1989 2531
Fragments arranged by size:
2531 1989 376 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2194 2226 3853
Size: 32 135 32 454 32 136 32 1627
Cuts at: 3853 3885 3927 3959 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1627 454 136 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2556 2802 669
Size: 988 281 618 246 3079
Fragments arranged by size:
3079 988 618 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5212
HpaI GTT'AAC
Cuts at: 2802 2802
Size: 5212
KpnI G_GTAC'C
Cuts at: 2537 2537
Size: 5212
MfeI C'AATT_G
Cuts at: 2811 2811
Size: 5212
MluI A'CGCG_T
Cuts at: 5088 5088
Size: 5212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4326 4510 1305
Size: 3021 184 2007
Fragments arranged by size:
3021 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2420 10
Size: 54 1425 343 588 2802
Fragments arranged by size:
2802 1425 588 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 3170 3529 3688 518
Size: 648 1158 846 359 159 2042
Fragments arranged by size:
2042 1158 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2129 2164 2416 2628 3305 4453 4698
Size: 408 115 35 252 212 677 1148 245
Cuts at: 4698 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 408 252 245 212 115
35
NaeI GCC'GGC
Cuts at: 2082 2082
Size: 5212
NcoI C'CATG_G
Cuts at: 513 1065 2004 2439 513
Size: 552 939 435 3286
Fragments arranged by size:
3286 939 552 435
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5212
NgoMIV G'CCGG_C
Cuts at: 2080 2080
Size: 5212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5212
NotI GC'GGCC_GC
Cuts at: 2659 2659
Size: 5212
NspI r_CATG'y
Cuts at: 1132 1229 2388 2570 4115 1132
Size: 97 1159 182 1545 2229
Fragments arranged by size:
2229 1545 1159 182 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5212
PciI A'CATG_T
Cuts at: 4111 4111
Size: 5212
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2256 1108
Size: 1148 4064
PmeI GTTT'AAAC
Cuts at: 2516 2516
Size: 5212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 4794 4826 3281
Size: 32 1481 32 3667
Fragments arranged by size:
3667 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5212
PsiI TTA'TAA
Cuts at: 2782 2782
Size: 5212
PstI C_TGCA'G
Cuts at: 830 2564 830
Size: 1734 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5212
PvuII CAG'CTG
Cuts at: 2164 2164
Size: 5212
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 5212
SalI G'TCGA_C
Cuts at: 1936 2554 1936
Size: 618 4594
SbfI CC_TGCA'GG
Cuts at: 2564 2564
Size: 5212
ScaI AGT'ACT
Cuts at: 3460 5172 3460
Size: 1712 3500
SfcI C'TryA_G
Cuts at: 826 1045 2560 3695 4376 4567 826
Size: 219 1515 1135 681 191 1471
Fragments arranged by size:
1515 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
SgrAI Cr'CCGG_yG
Cuts at: 1700 2080 1700
Size: 380 4832
SmaI CCC'GGG
Cuts at: 1872 2154 2539 1872
Size: 282 385 4545
Fragments arranged by size:
4545 385 282
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3316 4217 4479 820
Size: 197 926 450 923 901 262 1553
Fragments arranged by size:
1553 926 923 901 450 262 197
SnaBI TAC'GTA
Cuts at: 493 3003 493
Size: 2510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5212
SphI G_CATG'C
Cuts at: 1132 2388 2570 1132
Size: 1256 182 3774
Fragments arranged by size:
3774 1256 182
SspI AAT'ATT
Cuts at: 5 52 3136 5
Size: 47 3084 2081
Fragments arranged by size:
3084 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5212
StyI C'CwwG_G
Cuts at: 513 1065 2004 2439 2651 513
Size: 552 939 435 212 3074
Fragments arranged by size:
3074 939 552 435 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 3239 3256 3409 3594 4039 325
Size: 1255 450 1209 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1209 450 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3458 5170 96
Size: 274 80 33 51 1443 1481 1712 138
Fragments arranged by size:
1712 1481 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5172 5204 5172
Size: 32 5180
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5212
XbaI T'CTAG_A
Cuts at: 2548 2548
Size: 5212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2428
Size: 5212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5212
XmaI C'CCGG_G
Cuts at: 1870 2152 2537 1870
Size: 282 385 4545
Fragments arranged by size:
4545 385 282
XmnI GAAnn'nnTTC
Cuts at: 3341 3341
Size: 5212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3020 276
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI