Restriction map for
pFN21AB6356
(Circular) (Six-base) MAPSORT of: pFN21AB6356.seq Check: 9724 from: 1 to: 5590
pFN21AB6356 5590 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2073 2128 2911 2073
Size: 55 783 4752
Fragments arranged by size:
4752 783 55
AclI AA'CG_TT
Cuts at: 1563 2291 3717 4090 1563
Size: 728 1426 373 3063
Fragments arranged by size:
3063 1426 728 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3653 5037 5305 1632
Size: 2021 1384 268 1917
Fragments arranged by size:
2021 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 2841 4489 5466 1203
Size: 1638 1648 977 1327
Fragments arranged by size:
1648 1638 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AlwNI CAG_nnn'CTG
Cuts at: 2350 2490 3333 4905 2350
Size: 140 843 1572 3035
Fragments arranged by size:
3035 1572 843 140
ApaI G_GGCC'C
Cuts at: 2103 2634 2874 2103
Size: 531 240 4819
Fragments arranged by size:
4819 531 240
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2234 2899 3125 3277 5386 1493
Size: 741 665 226 152 2109 1697
Fragments arranged by size:
2109 1697 741 665 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2435 2682 2915 1870
Size: 73 159 333 247 233 4545
Fragments arranged by size:
4545 333 247 233 159 73
AvrII C'CTAG_G
Cuts at: 2296 2296
Size: 5590
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2110 2128 2218 2399
Size: 325 217 627 286 37 18 90 181
Cuts at: 2399 2534 2911 4366 618
Size: 135 377 1455 1842
Fragments arranged by size:
1842 1455 627 377 325 286 217 181
135 90 37 18
BanII G_rGCy'C
Cuts at: 1391 2103 2634 2874 2909 1391
Size: 712 531 240 35 4072
Fragments arranged by size:
4072 712 531 240 35
BbeI G_GCGC'C
Cuts at: 2114 2403 2114
Size: 289 5301
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2684 928
Size: 971 785 3834
Fragments arranged by size:
3834 971 785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 2251 1548
Size: 703 4887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2499 4201 136
Size: 107 122 71 631 605 827 1702 1525
Fragments arranged by size:
1702 1525 827 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 2277 3007 2277
Size: 730 4860
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2121 2634 2874 3651 4807 1790
Size: 313 18 513 240 777 1156 2573
Fragments arranged by size:
2573 1156 777 513 313 240 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5590
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3321 4279 442
Size: 416 53 83 399 24 1904 958 1753
Fragments arranged by size:
1904 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2762
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 4250 1239
Size: 1250 1761 2579
Fragments arranged by size:
2579 1761 1250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3715 4580 4878 3715
Size: 865 298 4427
Fragments arranged by size:
4427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2300 4253 882
Size: 617 801 1953 2219
Fragments arranged by size:
2219 1953 801 617
BsaAI yAC'GTr
Cuts at: 493 1204 2551 3381 493
Size: 711 1347 830 2702
Fragments arranged by size:
2702 1347 830 711
BsaBI GATnn'nnATC
Cuts at: 2601 5584 2601
Size: 2983 2607
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 2400 3397 3779
Size: 53 83 186 936 578 289 997 382
Cuts at: 3779 4414 275
Size: 635 1451
Fragments arranged by size:
1451 997 936 635 578 382 289 186
83 53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5365
Fragments arranged by size:
5365 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 2874 1186
Size: 1195 493 3902
Fragments arranged by size:
3902 1195 493
BseYI C'CCAG_C
Cuts at: 2137 2301 2815 2867 4793 5289 2137
Size: 164 514 52 1926 496 2438
Fragments arranged by size:
2438 1926 514 496 164 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 2456 1163
Size: 573 528 192 4297
Fragments arranged by size:
4297 573 528 192
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2364 2411 2605 3040 3801 4829
Size: 275 186 363 47 194 435 761 1028
Cuts at: 4829 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 435 363 275 194 186
47
BsiHKAI G_wGCw'C
Cuts at: 3651 3736 4807 3651
Size: 85 1071 4434
Fragments arranged by size:
4434 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2121 2634 2874 2909 3651 3736
Size: 399 313 18 513 240 35 742 85
Cuts at: 3736 4807 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 513 399 313 240 85
35 18
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 2945 844
Size: 1296 805 3489
Fragments arranged by size:
3489 1296 805
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4085 4259 65
Size: 1183 2837 174 1396
Fragments arranged by size:
2837 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2083 2406 4234 4421 1700
Size: 110 273 323 1828 187 2869
Fragments arranged by size:
2869 1828 323 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2379 2490 794
Size: 1585 111 3894
Fragments arranged by size:
3894 1585 111
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585
Size: 948 367 385 17 1452 86 12 346
Cuts at: 5585 1972
Size: 1977
Fragments arranged by size:
1977 1452 948 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2175 513
Size: 552 939 171 3928
Fragments arranged by size:
3928 939 552 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 2207 2704 2726 3106 3900 3920 2207
Size: 497 22 380 794 20 3877
Fragments arranged by size:
3877 794 497 380 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2408 2427
Size: 54 1425 50 275 18 531 47 19
Cuts at: 2427 2545 3037 3926 5555 8
Size: 118 492 889 1629 43
Fragments arranged by size:
1629 1425 889 531 492 275 118 54
50 47 43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2361 2408 3037 1537
Size: 275 549 47 629 4090
Fragments arranged by size:
4090 629 549 275 47
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 3520 1353
Size: 809 1358 3423
Fragments arranged by size:
3423 1358 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2411 2453 4177 4551 4697 387
Size: 1064 138 822 42 1724 374 146 1280
Fragments arranged by size:
1724 1280 1064 822 374 146 138 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 3653 4250 5037 5305 1239
Size: 393 857 1164 597 787 268 1524
Fragments arranged by size:
1524 1164 857 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2354
Size: 5590
EcoO109I rG'GnC_Cy
Cuts at: 2630 2631 2728 2871 2630
Size: 1 97 143 5349
Fragments arranged by size:
5349 143 97 1
EcoRI G'AATT_C
Cuts at: 2234 2899 3277 2234
Size: 665 378 4547
Fragments arranged by size:
4547 665 378
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 2090 2114 2403 2868 4737 1678
Size: 412 24 289 465 1869 2531
Fragments arranged by size:
2531 1869 465 412 289 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2299 2331 2551 2583 4231
Size: 32 135 32 727 32 220 32 1648
Cuts at: 4231 4263 4305 4337 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1648 727 220 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KasI G'GCGC_C
Cuts at: 2110 2399 2110
Size: 289 5301
KpnI G_GTAC'C
Cuts at: 2077 2132 2915 2077
Size: 55 783 4752
Fragments arranged by size:
4752 783 55
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2547 10
Size: 54 1425 343 715 3053
Fragments arranged by size:
3053 1425 715 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3548 3907 4066 518
Size: 648 2382 359 159 2042
Fragments arranged by size:
2382 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2413 2470 2487 2782 3006 3683 4831 5076
Size: 807 57 17 295 224 677 1148 245
Cuts at: 5076 1606
Size: 2120
Fragments arranged by size:
2120 1148 807 677 295 245 224 57
17
NaeI GCC'GGC
Cuts at: 2085 2408 2085
Size: 323 5267
NarI GG'CG_CC
Cuts at: 2111 2400 2111
Size: 289 5301
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NgoMIV G'CCGG_C
Cuts at: 2083 2406 2083
Size: 323 5267
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2845 2948 4493 1132
Size: 97 1616 103 1545 2229
Fragments arranged by size:
2229 1616 1545 103 97
PasI CC'CwG_GG
Cuts at: 1348 2069 2350 1348
Size: 721 281 4588
Fragments arranged by size:
4588 721 281
PciI A'CATG_T
Cuts at: 2841 4489 2841
Size: 1648 3942
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2512 1108
Size: 1404 4186
PfoI T'CCnGG_A
Cuts at: 2505 2592 2505
Size: 87 5503
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PmlI CAC'GTG
Cuts at: 2551 2551
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspOMI G'GGCC_C
Cuts at: 2099 2630 2870 2099
Size: 531 240 4819
Fragments arranged by size:
4819 531 240
PstI C_TGCA'G
Cuts at: 830 2486 2942 830
Size: 1656 456 3478
Fragments arranged by size:
3478 1656 456
PvuI CG_AT'CG
Cuts at: 2001 2605 2001
Size: 604 4986
PvuII CAG'CTG
Cuts at: 2487 2487
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2482 2555 2771 2938 4073 4754 4945
Size: 219 1437 73 216 167 1135 681 191
Cuts at: 4945 826
Size: 1471
Fragments arranged by size:
1471 1437 1135 681 219 216 191 167
73
SfoI GGC'GCC
Cuts at: 2112 2401 2112
Size: 289 5301
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2104 2437 2917 1872
Size: 232 333 480 4545
Fragments arranged by size:
4545 480 333 232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3694 4595 4857 820
Size: 197 926 1751 901 262 1553
Fragments arranged by size:
1751 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SrfI GCCC'GGGC
Cuts at: 2104 2104
Size: 5590
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2296 3029 513
Size: 552 939 177 115 733 3074
Fragments arranged by size:
3074 939 733 552 177 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3617 3634 3787 3972 4417 325
Size: 1255 2037 17 153 185 445 1498
Fragments arranged by size:
2037 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2518 3836 5548
Size: 274 80 33 51 1443 541 1318 1712
Cuts at: 5548 96
Size: 138
Fragments arranged by size:
1712 1443 1318 541 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5550 5582 5550
Size: 32 5558
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2102 2435 2915 1870
Size: 232 333 480 4545
Fragments arranged by size:
4545 480 333 232
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SwaI XcmI