Restriction map for
pFN21AB6353
(Circular) (Six-base) MAPSORT of: pFN21AB6353.seq Check: 7517 from: 1 to: 5503
pFN21AB6353 5503 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2615 2615
Size: 5503
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 1937
Size: 909 4594
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 3566 4950 5218 1632
Size: 404 1530 1384 268 1917
Fragments arranged by size:
1917 1530 1384 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 4402 5379 1203
Size: 3199 977 1327
Fragments arranged by size:
3199 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5503
AlwNI CAG_nnn'CTG
Cuts at: 2416 2563 3246 4818 2416
Size: 147 683 1572 3101
Fragments arranged by size:
3101 1572 683 147
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2812 3038 3190 5299 1493
Size: 1319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 226 152
AscI GG'CGCG_CC
Cuts at: 2305 2305
Size: 5503
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 1870
Size: 73 885 4545
Fragments arranged by size:
4545 885 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2824 4279 618
Size: 325 217 627 1037 1455 1842
Fragments arranged by size:
1842 1455 1037 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2075 2639 2822 1391
Size: 684 564 183 4072
Fragments arranged by size:
4072 684 564 183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2304 928
Size: 971 405 4127
Fragments arranged by size:
4127 971 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3694 3728 1903
Size: 34 1757 34 3678
Fragments arranged by size:
3678 1757 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3395 4605 2406
Size: 989 1210 3304
Fragments arranged by size:
3304 1210 989
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4114 136
Size: 107 122 71 631 605 2442 1525
Fragments arranged by size:
2442 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5498 5498
Size: 5503
BlpI GC'TnA_GC
Cuts at: 2069 2920 2069
Size: 851 4652
Bme1580I G_kGCm'C
Cuts at: 1790 2772 3564 4720 1790
Size: 982 792 1156 2573
Fragments arranged by size:
2573 1156 982 792
BmgBI CAC'GTC
Cuts at: 1318 2221 1318
Size: 903 4600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3234 4192 442
Size: 416 53 83 399 24 1817 958 1753
Fragments arranged by size:
1817 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2046 4163 1239
Size: 807 2117 2579
Fragments arranged by size:
2579 2117 807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4493 4791 3628
Size: 865 298 4340
Fragments arranged by size:
4340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4166 882
Size: 617 2667 2219
Fragments arranged by size:
2667 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3294 493
Size: 711 2090 2702
Fragments arranged by size:
2702 2090 711
BsaBI GATnn'nnATC
Cuts at: 5497 5497
Size: 5503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3310 3692 4327 275
Size: 53 83 186 936 1777 382 635 1451
Fragments arranged by size:
1777 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2487 3936 4334 4608 4755 5166 2487
Size: 1449 398 274 147 411 2824
Fragments arranged by size:
2824 1449 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5278
Fragments arranged by size:
5278 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4479
BseYI C'CCAG_C
Cuts at: 2090 2248 2510 2713 4706 5202 2090
Size: 158 262 203 1993 496 2391
Fragments arranged by size:
2391 1993 496 262 203 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2384 2658 1163
Size: 573 420 228 274 4008
Fragments arranged by size:
4008 573 420 274 228
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3714 4742 1540
Size: 275 186 952 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2075 2622 2662 3564 3649 4720 2075
Size: 547 40 902 85 1071 2858
Fragments arranged by size:
2858 1071 902 547 85 40
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2622 2639 2662 2772 2822 3564
Size: 399 285 547 17 23 110 50 742
Cuts at: 3564 3649 4720 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 399 285 110 85
50 23 17
BspEI T'CCGG_A
Cuts at: 2487 5166 2487
Size: 2679 2824
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2615 2858 844
Size: 1771 243 3489
Fragments arranged by size:
3489 1771 243
BsrBI CCG'CTC
Cuts at: 1394 1823 2158 3390 1394
Size: 429 335 1232 3507
Fragments arranged by size:
3507 1232 429 335
BsrDI GCAATG_nn'
Cuts at: 65 1248 3998 4172 65
Size: 1183 2750 174 1396
Fragments arranged by size:
2750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4147 4334 1700
Size: 110 2337 187 2869
Fragments arranged by size:
2869 2337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5503
BssHII G'CGCG_C
Cuts at: 1925 2305 1925
Size: 380 5123
BssSI C'ACGA_G
Cuts at: 2795 3563 4575 2795
Size: 768 1012 3723
Fragments arranged by size:
3723 1012 768
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5503
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstYI r'GATC_y
Cuts at: 1972 2833 3200 3585 3602 5054 5140 5152 5498
Size: 861 367 385 17 1452 86 12 346
Cuts at: 5498 1972
Size: 1977
Fragments arranged by size:
1977 1452 861 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2382 2382
Size: 5503
BtgI C'CryG_G
Cuts at: 513 1065 2004 2310 513
Size: 552 939 306 3706
Fragments arranged by size:
3706 939 552 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4764
Fragments arranged by size:
4764 683 56
BtsI GCAGTG_nn'
Cuts at: 3019 3813 3833 3019
Size: 794 20 4689
Fragments arranged by size:
4689 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2067 2094 2102 2129 529
Size: 35 1503 27 8 27 3903
Fragments arranged by size:
3903 1503 35 27 27 8
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4510 809
Size: 507 3194 1802
Fragments arranged by size:
3194 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2950 3839 5468
Size: 54 1425 50 275 18 1120 889 1629
Cuts at: 5468 8
Size: 43
Fragments arranged by size:
1629 1425 1120 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 2729 3433 1353
Size: 1143 233 704 3423
Fragments arranged by size:
3423 1143 704 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 4464 4610 387
Size: 1064 138 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2073 2073
Size: 5503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2046 3566 4163 4950 5218 1239
Size: 393 404 10 1520 597 787 268 1524
Fragments arranged by size:
1524 1520 787 597 404 393 268 10
EcoO109I rG'GnC_Cy
Cuts at: 2406 2406
Size: 5503
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
FspI TGC'GCA
Cuts at: 4009 4009
Size: 5503
HaeII r_GCGC'y
Cuts at: 1678 4650 1678
Size: 2972 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2380 2412 2623 2655 4144
Size: 32 135 32 808 32 211 32 1489
Cuts at: 4144 4176 4218 4250 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1489 808 211 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 3093 669
Size: 988 281 909 246 3079
Fragments arranged by size:
3079 988 909 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 3093 3093
Size: 5503
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 3102 3102
Size: 5503
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4801 1305
Size: 3312 184 2007
Fragments arranged by size:
3312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3681
Fragments arranged by size:
3681 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3461 3820 3979 518
Size: 648 2295 359 159 2042
Fragments arranged by size:
2295 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2312 2368 2919 3596 4744 4989 1606
Size: 706 56 551 677 1148 245 2120
Fragments arranged by size:
2120 1148 706 677 551 245 56
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4012
Fragments arranged by size:
4012 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2861 4406 1132
Size: 97 1632 1545 2229
Fragments arranged by size:
2229 1632 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2563 1108
Size: 1455 4048
PfoI T'CCnGG_A
Cuts at: 2292 2292
Size: 5503
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2406 2406
Size: 5503
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PstI C_TGCA'G
Cuts at: 830 2330 2421 2855 830
Size: 1500 91 434 3478
Fragments arranged by size:
3478 1500 434 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
SacI G_AGCT'C
Cuts at: 2075 2075
Size: 5503
SacII CC_GC'GG
Cuts at: 2313 2313
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SapI GCTCTTCn'nnn_
Cuts at: 2729 2729
Size: 5503
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 2270 3751 5463 2270
Size: 1481 1712 2310
Fragments arranged by size:
2310 1712 1481
SfcI C'TryA_G
Cuts at: 826 1045 2326 2417 2851 3986 4667 4858 826
Size: 219 1281 91 434 1135 681 191 1471
Fragments arranged by size:
1471 1281 1135 681 434 219 191 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2830 1872
Size: 958 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3607 4508 4770 820
Size: 197 926 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SspI AAT'ATT
Cuts at: 5 52 3427 5
Size: 47 3375 2081
Fragments arranged by size:
3375 2081 47
StuI AGG'CCT
Cuts at: 1517 2529 1517
Size: 1012 4491
StyI C'CwwG_G
Cuts at: 513 1065 2004 2728 2942 513
Size: 552 939 724 214 3074
Fragments arranged by size:
3074 939 724 552 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2637 2799 3530 3547 3700 3885 4330
Size: 1255 1057 162 731 17 153 185 445
Cuts at: 4330 325
Size: 1498
Fragments arranged by size:
1498 1255 1057 731 445 185 162 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 3749 5461
Size: 274 80 33 51 1443 291 1481 1712
Cuts at: 5461 96
Size: 138
Fragments arranged by size:
1712 1481 1443 291 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5503
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2828 1870
Size: 958 4545
XmnI GAAnn'nnTTC
Cuts at: 2288 2453 2580 3632 2288
Size: 165 127 1052 4159
Fragments arranged by size:
4159 1052 165 127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI