Restriction map for
pFN21AB6351
(Circular) (Six-base) MAPSORT of: pFN21AB6351.seq Check: 7107 from: 1 to: 5503
pFN21AB6351 5503 bp
With 182 enzymes: SgfI *
October 20, 2009 10:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 1937
Size: 909 4594
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3566 4950 5218 1632
Size: 1934 1384 268 1917
Fragments arranged by size:
1934 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 2800 4402 5379 1203
Size: 1597 1602 977 1327
Fragments arranged by size:
1602 1597 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5503
AlwNI CAG_nnn'CTG
Cuts at: 2177 3246 4818 2177
Size: 1069 1572 2862
Fragments arranged by size:
2862 1572 1069
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2812 3038 3190 5299 1493
Size: 1319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2209 2390 2828 1870
Size: 73 91 175 181 438 4545
Fragments arranged by size:
4545 438 181 175 91 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2824 4279 618
Size: 325 217 627 379 658 1455 1842
Fragments arranged by size:
1842 1455 658 627 379 325 217
BanII G_rGCy'C
Cuts at: 1391 2822 1391
Size: 1431 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 2520 928
Size: 971 516 105 3911
Fragments arranged by size:
3911 971 516 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2346 2380 3694 3728 1903
Size: 34 409 34 1314 34 3678
Fragments arranged by size:
3678 1314 409 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3395 4605 2736
Size: 659 1210 3634
Fragments arranged by size:
3634 1210 659
BclI T'GATC_A
Cuts at: 1548 2428 2485 1548
Size: 880 57 4566
Fragments arranged by size:
4566 880 57
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4114 136
Size: 107 122 71 631 605 337 2105 1525
Fragments arranged by size:
2105 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2628 5498 2628
Size: 2870 2633
BlpI GC'TnA_GC
Cuts at: 2920 2920
Size: 5503
Bme1580I G_kGCm'C
Cuts at: 1790 2169 3564 4720 1790
Size: 379 1395 1156 2573
Fragments arranged by size:
2573 1395 1156 379
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3234 4192 442
Size: 416 53 83 399 24 1817 958 1753
Fragments arranged by size:
1817 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4163 1239
Size: 2924 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4493 4791 3628
Size: 865 298 4340
Fragments arranged by size:
4340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4166 882
Size: 617 2667 2219
Fragments arranged by size:
2667 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3294 493
Size: 711 2090 2702
Fragments arranged by size:
2702 2090 711
BsaBI GATnn'nnATC
Cuts at: 2433 2757 5497 2433
Size: 324 2740 2439
Fragments arranged by size:
2740 2439 324
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3310 3692 4327 275
Size: 53 83 186 936 1777 382 635 1451
Fragments arranged by size:
1777 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2574 3936 4334 4608 4755 5166 2574
Size: 1362 398 274 147 411 2911
Fragments arranged by size:
2911 1362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5278
Fragments arranged by size:
5278 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2460 1186
Size: 1099 175 4229
Fragments arranged by size:
4229 1099 175
BseYI C'CCAG_C
Cuts at: 2083 2149 4706 5202 2083
Size: 66 2557 496 2384
Fragments arranged by size:
2557 2384 496 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4930
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3714 4742 1540
Size: 275 186 952 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3564 3649 4720 3564
Size: 85 1071 4347
Fragments arranged by size:
4347 1071 85
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2822 3564 3649 4720 1391
Size: 399 379 653 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 653 399 379 85
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 2858 844
Size: 1301 713 3489
Fragments arranged by size:
3489 1301 713
BsrBI CCG'CTC
Cuts at: 1394 1823 3390 1394
Size: 429 1567 3507
Fragments arranged by size:
3507 1567 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3998 4172 65
Size: 1183 2750 174 1396
Fragments arranged by size:
2750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4147 4334 1700
Size: 110 2337 187 2869
Fragments arranged by size:
2869 2337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5503
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5503
BssSI C'ACGA_G
Cuts at: 3563 4575 3563
Size: 1012 4491
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5503
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstYI r'GATC_y
Cuts at: 1972 2628 2833 3200 3585 3602 5054 5140 5152
Size: 656 205 367 385 17 1452 86 12
Cuts at: 5152 5498 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 656 385 367 346 205 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2593 2593
Size: 5503
BtgI C'CryG_G
Cuts at: 513 1065 2308 513
Size: 552 1243 3708
Fragments arranged by size:
3708 1243 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2063 487
Size: 683 56 837 3927
Fragments arranged by size:
3927 837 683 56
BtsI GCAGTG_nn'
Cuts at: 3019 3813 3833 3019
Size: 794 20 4689
Fragments arranged by size:
4689 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5468
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4510 809
Size: 507 3194 1802
Fragments arranged by size:
3194 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2950 3839
Size: 54 1425 50 275 18 241 879 889
Cuts at: 3839 5468 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 879 275 241 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3433 1353
Size: 2080 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 4464 4610 387
Size: 1064 138 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3566 4163 4950 5218 1239
Size: 393 1934 597 787 268 1524
Fragments arranged by size:
1934 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
FspI TGC'GCA
Cuts at: 2260 4009 2260
Size: 1749 3754
HaeII r_GCGC'y
Cuts at: 1678 2097 2160 2190 2356 4650 1678
Size: 419 63 30 166 2294 2531
Fragments arranged by size:
2531 2294 419 166 63 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2576 2608 4144 4176 4218
Size: 32 135 32 1004 32 1536 32 42
Cuts at: 4218 4250 1373
Size: 32 2626
Fragments arranged by size:
2626 1536 1004 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 3093 669
Size: 988 281 909 246 3079
Fragments arranged by size:
3079 988 909 281 246
HindIII A'AGCT_T
Cuts at: 748 2473 748
Size: 1725 3778
HpaI GTT'AAC
Cuts at: 3093 3093
Size: 5503
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 3102 3102
Size: 5503
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 4617 4801 1305
Size: 840 2472 184 2007
Fragments arranged by size:
2472 2007 840 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3681
Fragments arranged by size:
3681 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3461 3820 3979 518
Size: 648 2295 359 159 2042
Fragments arranged by size:
2295 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2149 2310 2919 3596 4744 4989 1606
Size: 418 125 161 609 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 609 418 245 161 125
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4951
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NruI TCG'CGA
Cuts at: 2048 2048
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2371 2861 4406 1132
Size: 97 1142 490 1545 2229
Fragments arranged by size:
2229 1545 1142 490 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5503
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PstI C_TGCA'G
Cuts at: 830 2182 2348 2855 830
Size: 1352 166 507 3478
Fragments arranged by size:
3478 1352 507 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
SacII CC_GC'GG
Cuts at: 2311 2311
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SbfI CC_TGCA'GG
Cuts at: 2348 2855 2348
Size: 507 4996
ScaI AGT'ACT
Cuts at: 2291 3751 5463 2291
Size: 1460 1712 2331
Fragments arranged by size:
2331 1712 1460
SfcI C'TryA_G
Cuts at: 826 1045 2178 2344 2851 3986 4667 4858 826
Size: 219 1133 166 507 1135 681 191 1471
Fragments arranged by size:
1471 1135 1133 681 507 219 191 166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2036 2211 2392 2830 1872
Size: 164 175 181 438 4545
Fragments arranged by size:
4545 438 181 175 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3607 4508 4770 820
Size: 197 926 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2371 2861 1132
Size: 1239 490 3774
Fragments arranged by size:
3774 1239 490
SspI AAT'ATT
Cuts at: 5 52 2703 3427 5
Size: 47 2651 724 2081
Fragments arranged by size:
2651 2081 724 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5503
StyI C'CwwG_G
Cuts at: 513 1065 2496 2942 513
Size: 552 1431 446 3074
Fragments arranged by size:
3074 1431 552 446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2184 3530 3547 3700 3885 4330 325
Size: 1255 604 1346 17 153 185 445 1498
Fragments arranged by size:
1498 1346 1255 604 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 3749 5461
Size: 274 80 33 51 1443 312 1460 1712
Cuts at: 5461 96
Size: 138
Fragments arranged by size:
1712 1460 1443 312 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 2403 2435 2606 2638 5463 5495 2258
Size: 32 113 32 171 32 2825 32 2266
Fragments arranged by size:
2825 2266 171 113 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5503
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2034 2209 2390 2828 1870
Size: 164 175 181 438 4545
Fragments arranged by size:
4545 438 181 175 164
XmnI GAAnn'nnTTC
Cuts at: 3632 3632
Size: 5503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI