Restriction map for
pFN21AB6345
(Circular) (Six-base) MAPSORT of: pFN21AB6345.seq Check: 8044 from: 1 to: 5500
pFN21AB6345 5500 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 1937
Size: 906 4594
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3563 4947 5215 1632
Size: 1931 1384 268 1917
Fragments arranged by size:
1931 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 4399 5376 1203
Size: 3196 977 1327
Fragments arranged by size:
3196 1327 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AlwNI CAG_nnn'CTG
Cuts at: 2182 3243 4815 2182
Size: 1061 1572 2867
Fragments arranged by size:
2867 1572 1061
ApaI G_GGCC'C
Cuts at: 2634 2634
Size: 5500
ApaLI G'TGCA_C
Cuts at: 3557 4713 3557
Size: 1156 4344
ApoI r'AATT_y
Cuts at: 1493 2809 3035 3187 5296 1493
Size: 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 2825 1870
Size: 73 400 482 4545
Fragments arranged by size:
4545 482 400 73
BamHI G'GATC_C
Cuts at: 2830 3197 2830
Size: 367 5133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 2329 2821 4276 618
Size: 325 217 627 507 35 492 1455 1842
Fragments arranged by size:
1842 1455 627 507 492 325 217 35
BanII G_rGCy'C
Cuts at: 1391 2096 2483 2634 2819 1391
Size: 705 387 151 185 4072
Fragments arranged by size:
4072 705 387 185 151
BbeI G_GCGC'C
Cuts at: 2298 2298
Size: 5500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2071 2783 928
Size: 971 172 712 3645
Fragments arranged by size:
3645 971 712 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3691 3725 1903
Size: 34 1754 34 3678
Fragments arranged by size:
3678 1754 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4602 3392
Size: 1210 4290
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2478 4111 136
Size: 107 122 71 631 605 806 1633 1525
Fragments arranged by size:
1633 1525 806 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2247 5495 2247
Size: 3248 2252
BlpI GC'TnA_GC
Cuts at: 2917 2917
Size: 5500
Bme1580I G_kGCm'C
Cuts at: 1790 2634 3561 4717 1790
Size: 844 927 1156 2573
Fragments arranged by size:
2573 1156 927 844
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2623 2758 3231
Size: 416 53 83 399 24 1206 135 473
Cuts at: 3231 4189 442
Size: 958 1753
Fragments arranged by size:
1753 1206 958 473 416 399 135 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4160 1239
Size: 2921 2579
Bpu10I CC'TnA_GC
Cuts at: 2693 2693
Size: 5500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2087 3625 4490 4788 2021
Size: 66 1538 865 298 2733
Fragments arranged by size:
2733 1538 865 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4163 882
Size: 617 2664 2219
Fragments arranged by size:
2664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2275 2730 3291 493
Size: 711 1071 455 561 2702
Fragments arranged by size:
2702 1071 711 561 455
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2295 3307 3689 4324
Size: 53 83 186 936 762 1012 382 635
Cuts at: 4324 275
Size: 1451
Fragments arranged by size:
1451 1012 936 762 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3933 4331 4605 4752 5163 3933
Size: 398 274 147 411 4270
Fragments arranged by size:
4270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5275
Fragments arranged by size:
5275 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2768 1186
Size: 1582 3918
BseYI C'CCAG_C
Cuts at: 2283 2485 2560 4703 5199 2283
Size: 202 75 2143 496 2584
Fragments arranged by size:
2584 2143 496 202 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4927
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2950 3711 4739 1540
Size: 275 186 949 761 1028 2301
Fragments arranged by size:
2301 1028 949 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 3561 3646 4717 2024
Size: 1537 85 1071 2807
Fragments arranged by size:
2807 1537 1071 85
BsmI GAATG_Cn'
Cuts at: 3011 3104 3011
Size: 93 5407
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2096 2483 2634 2819 3561 3646
Size: 399 234 72 387 151 185 742 85
Cuts at: 3646 4717 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 234 185 151
85 72
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 2589 3389 5119 2589
Size: 800 1730 2970
Fragments arranged by size:
2970 1730 800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2682 2855 844
Size: 1838 173 3489
Fragments arranged by size:
3489 1838 173
BsrBI CCG'CTC
Cuts at: 1394 1823 2196 2335 2568 2609 2770 3387 1394
Size: 429 373 139 233 41 161 617 3507
Fragments arranged by size:
3507 617 429 373 233 161 139 41
BsrDI GCAATG_nn'
Cuts at: 65 1248 2298 3995 4169 65
Size: 1183 1050 1697 174 1396
Fragments arranged by size:
1697 1396 1183 1050 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4144 4331 1700
Size: 110 2334 187 2869
Fragments arranged by size:
2869 2334 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 3560 4572 3560
Size: 1012 4488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5500
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 2404 2476 2224
Size: 180 72 5248
Fragments arranged by size:
5248 180 72
BstYI r'GATC_y
Cuts at: 1972 2247 2830 3197 3582 3599 5051 5137 5149
Size: 275 583 367 385 17 1452 86 12
Cuts at: 5149 5495 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 583 385 367 346 275 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2025 2397 2781 513
Size: 552 960 372 384 3232
Fragments arranged by size:
3232 960 552 384 372
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4761
Fragments arranged by size:
4761 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3810 3830 3016
Size: 794 20 4686
Fragments arranged by size:
4686 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4507 809
Size: 507 3191 1802
Fragments arranged by size:
3191 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2108 2157
Size: 54 1425 50 275 18 231 47 49
Cuts at: 2157 2200 2355 2712 2947 3836 5465 8
Size: 43 155 357 235 889 1629 43
Fragments arranged by size:
1629 1425 889 357 275 235 231 155
54 50 49 47 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2947 1537
Size: 275 1135 4090
Fragments arranged by size:
4090 1135 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2684 2760 3430 1353
Size: 1331 76 670 3423
Fragments arranged by size:
3423 1331 670 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4087 4461 4607 387
Size: 1064 138 2498 374 146 1280
Fragments arranged by size:
2498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3563 4160 4947 5215 1239
Size: 393 1931 597 787 268 1524
Fragments arranged by size:
1931 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2179 2186 2630 2179
Size: 7 444 5049
Fragments arranged by size:
5049 444 7
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2120 2298 4647 1678
Size: 442 178 2349 2531
Fragments arranged by size:
2531 2349 442 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4141 4173 4215 4247 1373
Size: 32 135 32 2569 32 42 32 2626
Fragments arranged by size:
2626 2569 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 2844 3090 669
Size: 988 281 424 482 246 3079
Fragments arranged by size:
3079 988 482 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5500
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KasI G'GCGC_C
Cuts at: 2294 2294
Size: 5500
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2509 4614 4798 1305
Size: 1204 2105 184 2007
Fragments arranged by size:
2105 2007 1204 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2063 2159 2714 10
Size: 54 1425 343 231 96 555 2796
Fragments arranged by size:
2796 1425 555 343 231 96 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 3458 3817 3976 518
Size: 648 1236 1056 359 159 2042
Fragments arranged by size:
2042 1236 1056 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2287 2428 2761 2916 3593 4741 4986 1606
Size: 681 141 333 155 677 1148 245 2120
Fragments arranged by size:
2120 1148 681 677 333 245 155 141
NarI GG'CG_CC
Cuts at: 2295 2295
Size: 5500
NcoI C'CATG_G
Cuts at: 513 1065 2397 2781 513
Size: 552 1332 384 3232
Fragments arranged by size:
3232 1332 552 384
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2858 4403 1132
Size: 97 1629 1545 2229
Fragments arranged by size:
2229 1629 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2182 2515 1348
Size: 834 333 4333
Fragments arranged by size:
4333 834 333
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2182 2290 2398 1108
Size: 1074 108 108 4210
Fragments arranged by size:
4210 1074 108 108
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 5082 5114 3569
Size: 32 1481 32 3955
Fragments arranged by size:
3955 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2186 2186
Size: 5500
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5500
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PspOMI G'GGCC_C
Cuts at: 2630 2630
Size: 5500
PstI C_TGCA'G
Cuts at: 830 2136 2852 830
Size: 1306 716 3478
Fragments arranged by size:
3478 1306 716
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
PvuII CAG'CTG
Cuts at: 2287 2428 2287
Size: 141 5359
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SanDI GG'GwC_CC
Cuts at: 2186 2186
Size: 5500
SapI GCTCTTCn'nnn_
Cuts at: 2684 2760 2684
Size: 76 5424
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 3748 5460 3748
Size: 1712 3788
SfcI C'TryA_G
Cuts at: 826 1045 2132 2848 3983 4664 4855 826
Size: 219 1087 716 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 716 681 219 191
SfoI GGC'GCC
Cuts at: 2296 2296
Size: 5500
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2345 2827 1872
Size: 473 482 4545
Fragments arranged by size:
4545 482 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 2102 3604 4505 4767 820
Size: 197 926 93 66 1502 901 262 1553
Fragments arranged by size:
1553 1502 926 901 262 197 93 66
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2858 1132
Size: 1726 3774
SspI AAT'ATT
Cuts at: 5 52 3424 5
Size: 47 3372 2081
Fragments arranged by size:
3372 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5500
StyI C'CwwG_G
Cuts at: 513 1065 2397 2781 2939 513
Size: 552 1332 384 158 3074
Fragments arranged by size:
3074 1332 552 384 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3527 3544 3697 3882 4327 325
Size: 1255 1947 17 153 185 445 1498
Fragments arranged by size:
1947 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3746 5458 96
Size: 274 80 33 51 1443 1769 1712 138
Fragments arranged by size:
1769 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5500
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2167
Size: 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2343 2825 1870
Size: 473 482 4545
Fragments arranged by size:
4545 482 473
XmnI GAAnn'nnTTC
Cuts at: 2597 3629 2597
Size: 1032 4468
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI
SfiI SrfI SwaI