Restriction map for
pFN21AB6342
(Circular) (Six-base) MAPSORT of: pFN21AB6342.seq Check: 8646 from: 1 to: 5578
pFN21AB6342 5578 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2695 2695
Size: 5578
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2921 1937
Size: 984 4594
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3641 5025 5293 1632
Size: 2009 1384 268 1917
Fragments arranged by size:
2009 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 4307 4307
Size: 5578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AlwNI CAG_nnn'CTG
Cuts at: 2644 3321 4893 2644
Size: 677 1572 3329
Fragments arranged by size:
3329 1572 677
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2887 3113 3265 5374 1493
Size: 1394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2116 2903 1870
Size: 73 173 787 4545
Fragments arranged by size:
4545 787 173 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2782 2899 4354 618
Size: 325 217 627 995 117 1455 1842
Fragments arranged by size:
1842 1455 995 627 325 217 117
BanII G_rGCy'C
Cuts at: 1391 2850 2897 1391
Size: 1459 47 4072
Fragments arranged by size:
4072 1459 47
BbeI G_GCGC'C
Cuts at: 2786 2786
Size: 5578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 928
Size: 971 930 3677
Fragments arranged by size:
3677 971 930
BbvCI CC'TCA_GC
Cuts at: 2460 2805 2460
Size: 345 5233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4189 136
Size: 107 122 71 631 605 2517 1525
Fragments arranged by size:
2517 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5573 5573
Size: 5578
BlpI GC'TnA_GC
Cuts at: 2432 2995 2432
Size: 563 5015
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2067 3639 4795 1790
Size: 240 37 1572 1156 2573
Fragments arranged by size:
2573 1572 1156 240 37
BmgBI CAC'GTC
Cuts at: 1318 2875 1318
Size: 1557 4021
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 4267 442
Size: 416 53 83 399 24 1892 958 1753
Fragments arranged by size:
1892 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2741 4238 1239
Size: 1502 1497 2579
Fragments arranged by size:
2579 1502 1497
Bpu10I CC'TnA_GC
Cuts at: 2180 2460 2805 2180
Size: 280 345 4953
Fragments arranged by size:
4953 345 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3703 4568 4866 3703
Size: 865 298 4415
Fragments arranged by size:
4415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2755 3369 493
Size: 711 1551 614 2702
Fragments arranged by size:
2702 1551 711 614
BsaBI GATnn'nnATC
Cuts at: 5572 5572
Size: 5578
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2318 2783 3385 3767
Size: 53 83 186 936 785 465 602 382
Cuts at: 3767 4402 275
Size: 635 1451
Fragments arranged by size:
1451 936 785 635 602 465 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4011 4409 4683 4830 5241 4011
Size: 398 274 147 411 4348
Fragments arranged by size:
4348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 1178
Size: 30 165 30 840 30 4483
Fragments arranged by size:
4483 840 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2248 2477 2738 2774 2843 2846 2849 2852
Size: 1062 229 261 36 69 3 3 3
Cuts at: 2852 2855 2858 1186
Size: 3 3 3906
Fragments arranged by size:
3906 1062 261 229 69 36 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2046 2360 4781 5277 2046
Size: 314 2421 496 2347
Fragments arranged by size:
2421 2347 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2503 2543 2720 1163
Size: 573 767 40 177 4021
Fragments arranged by size:
4021 767 573 177 40
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 2598 3028 3789 4817 1540
Size: 275 186 87 510 430 761 1028 2301
Fragments arranged by size:
2301 1028 761 510 430 275 186 87
BsiHKAI G_wGCw'C
Cuts at: 2186 2874 3639 3724 4795 2186
Size: 688 765 85 1071 2969
Fragments arranged by size:
2969 1071 765 688 85
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
BsmBI CGTCTCn'nnnn_
Cuts at: 2522 2522
Size: 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2067 2186 2850 2874 2897 3639
Size: 399 240 37 119 664 24 23 742
Cuts at: 3639 3724 4795 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 399 240 119 85
37 24 23
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 3467 5197 3467
Size: 1730 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2695 2933 844
Size: 1851 238 3489
Fragments arranged by size:
3489 1851 238
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2121 2436 3465 1394
Size: 429 188 110 315 1029 3507
Fragments arranged by size:
3507 1029 429 315 188 110
BsrDI GCAATG_nn'
Cuts at: 65 1248 2454 4073 4247 65
Size: 1183 1206 1619 174 1396
Fragments arranged by size:
1619 1396 1206 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2087 2335 4222 4409 1700
Size: 110 277 248 1887 187 2869
Fragments arranged by size:
2869 1887 277 248 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5578
BssHII G'CGCG_C
Cuts at: 1925 2153 1925
Size: 228 5350
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2707 794
Size: 1913 3665
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 2338 2662 1184
Size: 1154 324 4100
Fragments arranged by size:
4100 1154 324
BstYI r'GATC_y
Cuts at: 1972 2908 3275 3660 3677 5129 5215 5227 5573
Size: 936 367 385 17 1452 86 12 346
Cuts at: 5573 1972
Size: 1977
Fragments arranged by size:
1977 1452 936 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2478 2601 513
Size: 552 939 168 306 123 3490
Fragments arranged by size:
3490 939 552 306 168 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2530 487
Size: 683 56 1304 3535
Fragments arranged by size:
3535 1304 683 56
BtsI GCAGTG_nn'
Cuts at: 3094 3888 3908 3094
Size: 794 20 4764
Fragments arranged by size:
4764 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2536 4585 809
Size: 507 1220 2049 1802
Fragments arranged by size:
2049 1802 1220 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2089 2541
Size: 54 1425 50 275 18 255 4 452
Cuts at: 2541 2595 2736 3025 3914 5543 8
Size: 54 141 289 889 1629 43
Fragments arranged by size:
1629 1425 889 452 289 275 255 141
54 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2085 2595 3025 1537
Size: 275 273 510 430 4090
Fragments arranged by size:
4090 510 430 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 2009 2420 2685 3508 1353
Size: 656 411 265 823 3423
Fragments arranged by size:
3423 823 656 411 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4165 4539 4685 387
Size: 1064 138 2576 374 146 1280
Fragments arranged by size:
2576 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2741 3641 4238 5025 5293 1239
Size: 393 1109 900 597 787 268 1524
Fragments arranged by size:
1524 1109 900 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2489 2489
Size: 5578
EcoO109I rG'GnC_Cy
Cuts at: 2494 2510 2494
Size: 16 5562
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
FseI GG_CCGG'CC
Cuts at: 2091 2091
Size: 5578
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2036 2300 2556 2732 2786 4725 1678
Size: 358 264 256 176 54 1939 2531
Fragments arranged by size:
2531 1939 358 264 256 176 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4219 4251 4293 4325 1373
Size: 32 135 32 2647 32 42 32 2626
Fragments arranged by size:
2647 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3168 669
Size: 988 281 984 246 3079
Fragments arranged by size:
3079 988 984 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KasI G'GCGC_C
Cuts at: 2782 2782
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 3177 3177
Size: 5578
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2286 4692 4876 1305
Size: 981 2406 184 2007
Fragments arranged by size:
2406 2007 981 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2097 3536 3895 4054 518
Size: 648 931 1439 359 159 2042
Fragments arranged by size:
2042 1439 931 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 2788 2994 3671 4819 5064 1606
Size: 543 639 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 639 543 245 206
NaeI GCC'GGC
Cuts at: 2089 2089
Size: 5578
NarI GG'CG_CC
Cuts at: 2783 2783
Size: 5578
NcoI C'CATG_G
Cuts at: 513 1065 2004 2172 2478 513
Size: 552 939 168 306 3613
Fragments arranged by size:
3613 939 552 306 168
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NgoMIV G'CCGG_C
Cuts at: 2087 2087
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2746 2936 4481 1132
Size: 97 1517 190 1545 2229
Fragments arranged by size:
2229 1545 1517 190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5578
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5578
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PmlI CAC'GTG
Cuts at: 2755 2755
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2510 2510
Size: 5578
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2502 2930 830
Size: 1672 428 3478
Fragments arranged by size:
3478 1672 428
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
PvuII CAG'CTG
Cuts at: 2149 2149
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SapI GCTCTTCn'nnn_
Cuts at: 2420 2685 2420
Size: 265 5313
SbfI CC_TGCA'GG
Cuts at: 2502 2930 2502
Size: 428 5150
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SexAI A'CCwGG_T
Cuts at: 2265 2265
Size: 5578
SfcI C'TryA_G
Cuts at: 826 1045 2110 2498 2926 4061 4742 4933 826
Size: 219 1065 388 428 1135 681 191 1471
Fragments arranged by size:
1471 1135 1065 681 428 388 219 191
SfoI GGC'GCC
Cuts at: 2784 2784
Size: 5578
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3682 4583 4845 820
Size: 197 926 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 2798 1517
Size: 1281 4297
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 2478 2490 3017 513
Size: 552 939 168 306 12 527 3074
Fragments arranged by size:
3074 939 552 527 306 168 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 3605 3622 3775 3960 4405 325
Size: 1255 1101 924 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1101 924 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3824 5536 96
Size: 274 80 33 51 1443 1847 1712 138
Fragments arranged by size:
1847 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5538 5570 5538
Size: 32 5546
Tth111I GACn'n_nGTC
Cuts at: 1316 2317 1316
Size: 1001 4577
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 2129 2447 3707 2129
Size: 318 1260 4000
Fragments arranged by size:
4000 1260 318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI