Restriction map for
pFN21AB6340
(Circular) (Six-base) MAPSORT of: pFN21AB6340.seq Check: 2349 from: 1 to: 5575
pFN21AB6340 5575 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 3638 5022 5290 1632
Size: 617 1389 1384 268 1917
Fragments arranged by size:
1917 1389 1384 617 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 2572 2618 2758 3318 4890 2572
Size: 46 140 560 1572 3257
Fragments arranged by size:
3257 1572 560 140 46
ApaI G_GGCC'C
Cuts at: 2750 2750
Size: 5575
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2884 3110 3262 5371 1493
Size: 1391 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2840 4129 160
Size: 872 1808 1289 1606
Fragments arranged by size:
1808 1606 1289 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2597 2900 1870
Size: 73 654 303 4545
Fragments arranged by size:
4545 654 303 73
BamHI G'GATC_C
Cuts at: 2562 2905 3272 2562
Size: 343 367 4865
Fragments arranged by size:
4865 367 343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 2896 4351 618
Size: 325 217 627 1079 30 1455 1842
Fragments arranged by size:
1842 1455 1079 627 325 217 30
BanII G_rGCy'C
Cuts at: 1391 2109 2427 2522 2701 2750 2894 1391
Size: 718 318 95 179 49 144 4072
Fragments arranged by size:
4072 718 318 179 144 95 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4604
BbvCI CC'TCA_GC
Cuts at: 2154 2154
Size: 5575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2540 2574 3766 3800 1903
Size: 34 603 34 1192 34 3678
Fragments arranged by size:
3678 1192 603 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 4693
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4186 136
Size: 107 122 71 631 605 2514 1525
Fragments arranged by size:
2514 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2036 2992 2036
Size: 956 4619
Bme1580I G_kGCm'C
Cuts at: 1790 2750 3636 4792 1790
Size: 960 886 1156 2573
Fragments arranged by size:
2573 1156 960 886
BmgBI CAC'GTC
Cuts at: 1318 2245 1318
Size: 927 4648
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3306 4264 442
Size: 416 53 83 399 24 1889 958 1753
Fragments arranged by size:
1889 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2093 2735 4235 1239
Size: 812 42 642 1500 2579
Fragments arranged by size:
2579 1500 812 642 42
Bpu10I CC'TnA_GC
Cuts at: 2154 2154
Size: 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2474 3700 4565 4863 2474
Size: 1226 865 298 3186
Fragments arranged by size:
3186 1226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 2435 5569 2435
Size: 3134 2441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2391 2482 2823 4008 4406 4680 4827 5238 2391
Size: 91 341 1185 398 274 147 411 2728
Fragments arranged by size:
2728 1185 411 398 341 274 147 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5350
Fragments arranged by size:
5350 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2173 2223 2267 2270 2291 2651 1186
Size: 907 80 50 44 3 21 360 4110
Fragments arranged by size:
4110 907 360 80 50 44 21 3
BseYI C'CCAG_C
Cuts at: 2023 2288 2572 2751 4778 5274 2023
Size: 265 284 179 2027 496 2324
Fragments arranged by size:
2324 2027 496 284 265 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 3025 3786 4814 1540
Size: 275 186 54 970 761 1028 2301
Fragments arranged by size:
2301 1028 970 761 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 2109 2427 3636 3721 4792 2109
Size: 318 1209 85 1071 2892
Fragments arranged by size:
2892 1209 1071 318 85
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2427 2522 2701 2750 2894 3636
Size: 399 319 318 95 179 49 144 742
Cuts at: 3636 3721 4792 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 318 179 144
95 85 49
BspEI T'CCGG_A
Cuts at: 2482 5238 2482
Size: 2756 2819
BspHI T'CATG_A
Cuts at: 2800 3464 5194 2800
Size: 664 1730 3181
Fragments arranged by size:
3181 1730 664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2544 3462 1394
Size: 429 721 918 3507
Fragments arranged by size:
3507 918 721 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2505 2863 4219 4406 1700
Size: 110 695 358 1356 187 2869
Fragments arranged by size:
2869 1356 695 358 187 110
BsrGI T'GTAC_A
Cuts at: 96 2583 96
Size: 2487 3088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 2426 3635 4647 2426
Size: 1209 1012 3354
Fragments arranged by size:
3354 1209 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2311
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2562 2905 3272 3657 3674 5126 5212 5224
Size: 590 343 367 385 17 1452 86 12
Cuts at: 5224 5570 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 590 385 367 346 343 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2422 487
Size: 683 56 1196 3640
Fragments arranged by size:
3640 1196 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3885 3905 3091
Size: 794 20 4761
Fragments arranged by size:
4761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2372 4582 809
Size: 507 1056 2210 1802
Fragments arranged by size:
2210 1802 1056 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2507 2667 2847
Size: 54 1425 50 275 18 677 160 180
Cuts at: 2847 3022 3911 5540 8
Size: 175 889 1629 43
Fragments arranged by size:
1629 1425 889 677 275 180 175 160
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2822 3505 1353
Size: 1469 683 3423
Fragments arranged by size:
3423 1469 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 4162 4536 4682 387
Size: 1064 138 471 2102 374 146 1280
Fragments arranged by size:
2102 1280 1064 471 374 146 138
EcoICRI GAG'CTC
Cuts at: 2107 2425 2107
Size: 318 5257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2093 2249 2735 3638 4235 5022
Size: 393 419 42 156 486 903 597 787
Cuts at: 5022 5290 1239
Size: 268 1524
Fragments arranged by size:
1524 903 787 597 486 419 393 268
156 42
EcoO109I rG'GnC_Cy
Cuts at: 2449 2578 2615 2746 2747 2449
Size: 129 37 131 1 5277
Fragments arranged by size:
5277 131 129 37 1
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 5575
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2782 2814 4216 4248 4290
Size: 32 135 32 1210 32 1402 32 42
Cuts at: 4290 4322 1373
Size: 32 2626
Fragments arranged by size:
2626 1402 1210 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2377 2919 3165 669
Size: 988 281 439 542 246 3079
Fragments arranged by size:
3079 988 542 439 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4689 4873 1305
Size: 3384 184 2007
Fragments arranged by size:
3384 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2849 10
Size: 54 1425 343 1017 2736
Fragments arranged by size:
2736 1425 1017 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 3533 3892 4051 518
Size: 648 1137 1230 359 159 2042
Fragments arranged by size:
2042 1230 1137 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2476 2758 2785 2991 3668 4816 5061 1606
Size: 870 282 27 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 677 282 245 206 27
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2933 4478 1132
Size: 97 1704 1545 2229
Fragments arranged by size:
2229 1704 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2701 1348
Size: 1353 4222
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 1108
Size: 1464 4111
PfoI T'CCnGG_A
Cuts at: 2565 2565
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 3644 3676 5157 5189 2528
Size: 32 1084 32 1481 32 2914
Fragments arranged by size:
2914 1481 1084 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 2578
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PspOMI G'GGCC_C
Cuts at: 2746 2746
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2094 2790 2927 830
Size: 1264 696 137 3478
Fragments arranged by size:
3478 1264 696 137
PvuI CG_AT'CG
Cuts at: 2001 2055 2001
Size: 54 5521
PvuII CAG'CTG
Cuts at: 2476 2758 2476
Size: 282 5293
SacI G_AGCT'C
Cuts at: 2109 2427 2109
Size: 318 5257
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2090 2786 2923 4058 4739 4930 826
Size: 219 1045 696 137 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 696 681 219 191 137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2599 2902 1872
Size: 727 303 4545
Fragments arranged by size:
4545 727 303
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 3679 4580 4842 820
Size: 197 926 510 1226 901 262 1553
Fragments arranged by size:
1553 1226 926 901 510 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 2096 1517
Size: 579 4996
StyI C'CwwG_G
Cuts at: 513 1065 2004 3014 513
Size: 552 939 1010 3074
Fragments arranged by size:
3074 1010 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2264 3602 3619 3772 3957 4402 325
Size: 1255 684 1338 17 153 185 445 1498
Fragments arranged by size:
1498 1338 1255 684 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2583 3821 5533
Size: 274 80 33 51 1443 606 1238 1712
Cuts at: 5533 96
Size: 138
Fragments arranged by size:
1712 1443 1238 606 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2767 2911 2767
Size: 144 5431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2156 2156
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2597 2900 1870
Size: 727 303 4545
Fragments arranged by size:
4545 727 303
XmnI GAAnn'nnTTC
Cuts at: 2198 3704 2198
Size: 1506 4069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI