Restriction map for
pFN21AB6332
(Circular) (Six-base) MAPSORT of: pFN21AB6332.seq Check: 7366 from: 1 to: 5569
pFN21AB6332 5569 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2912 1937
Size: 975 4594
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2700 3632 5016 5284 1632
Size: 1068 932 1384 268 1917
Fragments arranged by size:
1917 1384 1068 932 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 2699 2724 2737 3312 4884 2699
Size: 25 13 575 1572 3384
Fragments arranged by size:
3384 1572 575 25 13
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2793 2878 3104 3256 5365 1493
Size: 1300 85 226 152 2109 1697
Fragments arranged by size:
2109 1697 1300 226 152 85
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2894 1870
Size: 73 951 4545
Fragments arranged by size:
4545 951 73
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2890 4345 618
Size: 325 217 627 1103 1455 1842
Fragments arranged by size:
1842 1455 1103 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2888 1391
Size: 1497 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 2688 928
Size: 971 207 582 3809
Fragments arranged by size:
3809 971 582 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5569
BfrBI ATG'CAT
Cuts at: 2033 2107 2755 2033
Size: 74 648 4847
Fragments arranged by size:
4847 648 74
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 2620 4180
Size: 107 122 71 631 605 583 365 1560
Cuts at: 4180 136
Size: 1525
Fragments arranged by size:
1560 1525 631 605 583 365 122 107
71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 2858 3630 4786 1790
Size: 1068 772 1156 2573
Fragments arranged by size:
2573 1156 1068 772
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2681 3300 4258
Size: 416 53 83 399 24 1264 619 958
Cuts at: 4258 442
Size: 1753
Fragments arranged by size:
1753 1264 958 619 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2454 2546 4229 1239
Size: 1215 92 1683 2579
Fragments arranged by size:
2579 1683 1215 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2632 3694 4559 4857 2632
Size: 1062 865 298 3344
Fragments arranged by size:
3344 1062 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3376 3758 4393 275
Size: 53 83 186 936 1843 382 635 1451
Fragments arranged by size:
1843 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4002 4400 4674 4821 5232 4002
Size: 398 274 147 411 4339
Fragments arranged by size:
4339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5344
Fragments arranged by size:
5344 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 2793 2796 2844 1186
Size: 1291 316 3 48 3911
Fragments arranged by size:
3911 1291 316 48 3
BseYI C'CCAG_C
Cuts at: 2730 2788 4772 5268 2730
Size: 58 1984 496 3031
Fragments arranged by size:
3031 1984 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2685 2856 1163
Size: 573 411 538 171 3876
Fragments arranged by size:
3876 573 538 411 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3630 3715 4786 3630
Size: 85 1071 4413
Fragments arranged by size:
4413 1071 85
BsmI GAATG_Cn'
Cuts at: 2033 3080 3173 2033
Size: 1047 93 4429
Fragments arranged by size:
4429 1047 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2858 2888 3630 3715 4786 1391
Size: 399 1068 30 742 85 1071 2174
Fragments arranged by size:
2174 1071 1068 742 399 85 30
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2924 844
Size: 2080 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3456 1394
Size: 429 1633 3507
Fragments arranged by size:
3507 1633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4213 4400 1700
Size: 110 2403 187 2869
Fragments arranged by size:
2869 2403 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5569
BstYI r'GATC_y
Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564
Size: 927 367 385 17 1452 86 12 346
Cuts at: 5564 1972
Size: 1977
Fragments arranged by size:
1977 1452 927 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2663 513
Size: 552 1598 3419
Fragments arranged by size:
3419 1598 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 3879 3899 3085
Size: 794 20 4755
Fragments arranged by size:
4755 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DraIII CAC_nnn'GTG
Cuts at: 2240 2240
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2360 4576 809
Size: 507 1044 2216 1802
Fragments arranged by size:
2216 1802 1044 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2242 3016 3905
Size: 54 1425 50 275 18 412 774 889
Cuts at: 3905 5534 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 774 412 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3499 1353
Size: 2146 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2483 4156 4530 4676 387
Size: 1064 138 894 1673 374 146 1280
Fragments arranged by size:
1673 1280 1064 894 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2546 2700 3632 4229 5016 5284
Size: 393 822 92 154 932 597 787 268
Cuts at: 5284 1239
Size: 1524
Fragments arranged by size:
1524 932 822 787 597 393 268 154
92
EcoO109I rG'GnC_Cy
Cuts at: 2230 2230
Size: 5569
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 4716 1678
Size: 3038 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2630 2662 4210 4242 4284
Size: 32 135 32 1058 32 1548 32 42
Cuts at: 4284 4316 1373
Size: 32 2626
Fragments arranged by size:
2626 1548 1058 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2821 2913 3159 669
Size: 988 281 883 92 246 3079
Fragments arranged by size:
3079 988 883 281 246 92
HindIII A'AGCT_T
Cuts at: 748 2009 748
Size: 1261 4308
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4683 4867 1305
Size: 3378 184 2007
Fragments arranged by size:
3378 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2244 10
Size: 54 1425 343 412 3335
Fragments arranged by size:
3335 1425 412 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3527 3886 4045 518
Size: 648 2361 359 159 2042
Fragments arranged by size:
2361 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2267 2840 2985 3662 4810 5055 1606
Size: 661 573 145 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 661 573 245 145
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5017
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NsiI A_TGCA'T
Cuts at: 2035 2109 2757 2035
Size: 74 648 4847
Fragments arranged by size:
4847 648 74
NspI r_CATG'y
Cuts at: 1132 1229 2107 2759 2927 4472 1132
Size: 97 878 652 168 1545 2229
Fragments arranged by size:
2229 1545 878 652 168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2240 2737 1108
Size: 1132 497 3940
Fragments arranged by size:
3940 1132 497
PfoI T'CCnGG_A
Cuts at: 2364 2451 2364
Size: 87 5482
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2230
Size: 5569
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2266 2921 830
Size: 1436 655 3478
Fragments arranged by size:
3478 1436 655
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2267 2840 2267
Size: 573 4996
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2262 2917 4052 4733 4924 826
Size: 219 1217 655 1135 681 191 1471
Fragments arranged by size:
1471 1217 1135 681 655 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2647 3673 4574 4836 820
Size: 197 926 704 1026 901 262 1553
Fragments arranged by size:
1553 1026 926 901 704 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2759 2927 1132
Size: 1627 168 3774
Fragments arranged by size:
3774 1627 168
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 3008 513
Size: 552 1943 3074
Fragments arranged by size:
3074 1943 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3596 3613 3766 3951 4396 325
Size: 1255 2016 17 153 185 445 1498
Fragments arranged by size:
2016 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3815 5527 96
Size: 274 80 33 51 1443 1838 1712 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5529 5561 5529
Size: 32 5537
Tth111I GACn'n_nGTC
Cuts at: 1316 2695 1316
Size: 1379 4190
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI