Restriction map for
pFN21AB6329
(Circular) (Six-base) MAPSORT of: pFN21AB6329.seq Check: 3292 from: 1 to: 5488
pFN21AB6329 5488 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2638 2638
Size: 5488
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2385 2831 1937
Size: 448 446 4594
Fragments arranged by size:
4594 448 446
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3551 4935 5203 1632
Size: 1919 1384 268 1917
Fragments arranged by size:
1919 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 2388 2751 4387 5364 1203
Size: 1185 363 1636 977 1327
Fragments arranged by size:
1636 1327 1185 977 363
AgeI A'CCGG_T
Cuts at: 2665 4319 2665
Size: 1654 3834
AhdI GACnn_n'nnGTC
Cuts at: 2080 4217 2080
Size: 2137 3351
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 2492 3231 4803 2492
Size: 739 1572 3177
Fragments arranged by size:
3177 1572 739
ApaI G_GGCC'C
Cuts at: 2057 2527 2057
Size: 470 5018
ApaLI G'TGCA_C
Cuts at: 2309 3545 4701 2309
Size: 1236 1156 3096
Fragments arranged by size:
3096 1236 1156
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2813 1870
Size: 73 106 764 4545
Fragments arranged by size:
4545 764 106 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 2207 2809 4264 618
Size: 325 217 627 395 25 602 1455 1842
Fragments arranged by size:
1842 1455 627 602 395 325 217 25
BanII G_rGCy'C
Cuts at: 1391 2057 2439 2527 2706 2807 1391
Size: 666 382 88 179 101 4072
Fragments arranged by size:
4072 666 382 179 101 88
BbeI G_GCGC'C
Cuts at: 2186 2211 2186
Size: 25 5463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2542 2635 928
Size: 971 643 93 3781
Fragments arranged by size:
3781 971 643 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2526 2560 3679 3713 1903
Size: 34 589 34 1119 34 3678
Fragments arranged by size:
3678 1119 589 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2522 4099 136
Size: 107 122 71 631 605 850 1577 1525
Fragments arranged by size:
1577 1525 850 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2274 5483 2274
Size: 3209 2279
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2313 2527 3549 4705 1790
Size: 267 256 214 1022 1156 2573
Fragments arranged by size:
2573 1156 1022 267 256 214
BmgBI CAC'GTC
Cuts at: 1318 2752 1318
Size: 1434 4054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2222 2480 3219
Size: 416 53 83 399 24 805 258 739
Cuts at: 3219 4177 442
Size: 958 1753
Fragments arranged by size:
1753 958 805 739 416 399 258 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2466 1056
Size: 1410 4078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4148 1239
Size: 2909 2579
Bpu10I CC'TnA_GC
Cuts at: 2217 2579 2765 2217
Size: 362 186 4940
Fragments arranged by size:
4940 362 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3613 4478 4776 3613
Size: 865 298 4325
Fragments arranged by size:
4325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 2589 4151 882
Size: 617 795 295 1562 2219
Fragments arranged by size:
2219 1562 795 617 295
BsaAI yAC'GTr
Cuts at: 493 1204 2389 2569 3279 493
Size: 711 1185 180 710 2702
Fragments arranged by size:
2702 1185 711 710 180
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2183 2208 3295 3677
Size: 53 83 186 936 650 25 1087 382
Cuts at: 3677 4312 275
Size: 635 1451
Fragments arranged by size:
1451 1087 936 650 635 382 186 83
53 25
BsaWI w'CCGG_w
Cuts at: 2665 3921 4319 4593 4740 5151 2665
Size: 1256 398 274 147 411 3002
Fragments arranged by size:
3002 1256 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5263
Fragments arranged by size:
5263 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2213 2705 1186
Size: 871 156 492 3969
Fragments arranged by size:
3969 871 492 156
BseYI C'CCAG_C
Cuts at: 4691 5187 4691
Size: 496 4992
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4915
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2938 3699 4727 1540
Size: 275 186 18 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2313 2439 2613 2706 2766 3549 3634 4705 2313
Size: 126 174 93 60 783 85 1071 3096
Fragments arranged by size:
3096 1071 783 174 126 93 85 60
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2313 2439 2527 2613 2706 2766
Size: 399 267 256 126 88 86 93 60
Cuts at: 2766 2807 3549 3634 4705 1391
Size: 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 267 256 126 93
88 86 85 60 41
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2247 2505 2607 2638 2843 844
Size: 1403 258 102 31 205 3489
Fragments arranged by size:
3489 1403 258 205 102 31
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2583 2665 4132 4319 1700
Size: 110 773 82 1467 187 2869
Fragments arranged by size:
2869 1467 773 187 110 82
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 2674 3548 4560 2674
Size: 874 1012 3602
Fragments arranged by size:
3602 1012 874
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 2072 1184
Size: 888 4600
BstYI r'GATC_y
Cuts at: 1972 2274 2818 3185 3570 3587 5039 5125 5137
Size: 302 544 367 385 17 1452 86 12
Cuts at: 5137 5483 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 544 385 367 346 302 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2247 2247
Size: 5488
BtgI C'CryG_G
Cuts at: 513 1065 2004 2774 513
Size: 552 939 770 3227
Fragments arranged by size:
3227 939 770 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 2457 3004 3798 3818 2457
Size: 547 794 20 4127
Fragments arranged by size:
4127 794 547 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2777 2935
Size: 54 1425 50 275 18 186 761 158
Cuts at: 2935 3824 5453 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 761 275 186 158 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2935 1537
Size: 275 204 919 4090
Fragments arranged by size:
4090 919 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 3418 1353
Size: 782 1283 3423
Fragments arranged by size:
3423 1283 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2437 2704 2437
Size: 267 5221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3551 4148 4935 5203 1239
Size: 393 1919 597 787 268 1524
Fragments arranged by size:
1919 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2053 2161 2514 2575 2053
Size: 108 353 61 4966
Fragments arranged by size:
4966 353 108 61
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 2186 2211 4635 1678
Size: 508 25 2424 2531
Fragments arranged by size:
2531 2424 508 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2741 2773 4129
Size: 32 135 32 494 32 643 32 1356
Cuts at: 4129 4161 4203 4235 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 643 494 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KasI G'GCGC_C
Cuts at: 2182 2207 2182
Size: 25 5463
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2409 3446 3805 3964 518
Size: 648 1188 55 1037 359 159 2042
Fragments arranged by size:
2042 1188 1037 648 359 159 55
MspA1I CmG'CkG
Cuts at: 1606 2059 2178 2904 3581 4729 4974 1606
Size: 453 119 726 677 1148 245 2120
Fragments arranged by size:
2120 1148 726 677 453 245 119
NarI GG'CG_CC
Cuts at: 2183 2208 2183
Size: 25 5463
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 2462 1052
Size: 1410 4078
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2846 4391 1132
Size: 97 1617 1545 2229
Fragments arranged by size:
2229 1617 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5488
PfoI T'CCnGG_A
Cuts at: 2616 2616
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2575 2575
Size: 5488
PshAI GACnn'nnGTC
Cuts at: 1613 2228 1613
Size: 615 4873
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PspOMI G'GGCC_C
Cuts at: 2053 2523 2053
Size: 470 5018
PstI C_TGCA'G
Cuts at: 830 2424 2840 830
Size: 1594 416 3478
Fragments arranged by size:
3478 1594 416
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
SacI G_AGCT'C
Cuts at: 2439 2706 2439
Size: 267 5221
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SexAI A'CCwGG_T
Cuts at: 2379 2379
Size: 5488
SfcI C'TryA_G
Cuts at: 826 1045 2420 2836 3971 4652 4843 826
Size: 219 1375 416 1135 681 191 1471
Fragments arranged by size:
1471 1375 1135 681 416 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2522 2522
Size: 5488
SfoI GGC'GCC
Cuts at: 2184 2209 2184
Size: 25 5463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3592 4493 4755 820
Size: 197 926 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5488
StyI C'CwwG_G
Cuts at: 513 1065 2004 2253 2927 513
Size: 552 939 249 674 3074
Fragments arranged by size:
3074 939 674 552 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3532 3685 3870 4315 325
Size: 1255 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 3734 5446
Size: 274 80 33 51 1443 777 980 1712
Cuts at: 5446 96
Size: 138
Fragments arranged by size:
1712 1443 980 777 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacII SanDI SapI SrfI
SwaI XcmI