Restriction map for
pFN21AB6328
(Circular) (Six-base) MAPSORT of: pFN21AB6328.seq Check: 7683 from: 1 to: 5398
pFN21AB6328 5398 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3208 278
Size: 53 83 186 936 1672 2468
Fragments arranged by size:
2468 1672 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2741 1937
Size: 804 4594
Acc65I G'GTAC_C
Cuts at: 2719 2719
Size: 5398
AclI AA'CG_TT
Cuts at: 1563 3525 3898 1563
Size: 1962 373 3063
Fragments arranged by size:
3063 1962 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2044 2119 3461 4845 5113 1632
Size: 412 75 1342 1384 268 1917
Fragments arranged by size:
1917 1384 1342 412 268 75
AflII C'TTAA_G
Cuts at: 820 1017 2249 820
Size: 197 1232 3969
Fragments arranged by size:
3969 1232 197
AflIII A'CryG_T
Cuts at: 1203 4297 5274 1203
Size: 3094 977 1327
Fragments arranged by size:
3094 1327 977
AgeI A'CCGG_T
Cuts at: 4229 4229
Size: 5398
AhdI GACnn_n'nnGTC
Cuts at: 4127 4127
Size: 5398
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5398
AlwNI CAG_nnn'CTG
Cuts at: 2189 3141 4713 2189
Size: 952 1572 2874
Fragments arranged by size:
2874 1572 952
ApaLI G'TGCA_C
Cuts at: 3455 4611 3455
Size: 1156 4242
ApoI r'AATT_y
Cuts at: 1493 2707 2933 3085 5194 1493
Size: 1214 226 152 2109 1697
Fragments arranged by size:
2109 1697 1214 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3952 160
Size: 872 2920 1606
Fragments arranged by size:
2920 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5398
AvaI C'yCGr_G
Cuts at: 1870 1943 2694 2723 1870
Size: 73 751 29 4545
Fragments arranged by size:
4545 751 73 29
AvrII C'CTAG_G
Cuts at: 2606 2606
Size: 5398
BamHI G'GATC_C
Cuts at: 2728 3095 2728
Size: 367 5031
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2719 4174 618
Size: 325 217 627 932 1455 1842
Fragments arranged by size:
1842 1455 932 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2345 2717 1391
Size: 954 372 4072
Fragments arranged by size:
4072 954 372
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3589 3623 1903
Size: 34 1652 34 3678
Fragments arranged by size:
3678 1652 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2070 2265 3290 4500 2070
Size: 195 1025 1210 2968
Fragments arranged by size:
2968 1210 1025 195
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5398
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4009 136
Size: 107 122 71 631 605 2337 1525
Fragments arranged by size:
2337 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5393 5393
Size: 5398
BlpI GC'TnA_GC
Cuts at: 2815 2815
Size: 5398
Bme1580I G_kGCm'C
Cuts at: 1790 3459 4615 1790
Size: 1669 1156 2573
Fragments arranged by size:
2573 1669 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5398
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2347 3129 4087
Size: 416 53 83 399 24 930 782 958
Cuts at: 4087 442
Size: 1753
Fragments arranged by size:
1753 958 930 782 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2619 4058 1239
Size: 1380 1439 2579
Fragments arranged by size:
2579 1439 1380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3523 4388 4686 3523
Size: 865 298 4235
Fragments arranged by size:
4235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4061 882
Size: 617 2562 2219
Fragments arranged by size:
2562 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3189 493
Size: 711 1985 2702
Fragments arranged by size:
2702 1985 711
BsaBI GATnn'nnATC
Cuts at: 5392 5392
Size: 5398
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3205 3587 4222 275
Size: 53 83 186 936 1672 382 635 1451
Fragments arranged by size:
1672 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2688 3831 4229 4503 4650 5061 2688
Size: 1143 398 274 147 411 3025
Fragments arranged by size:
3025 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2357 1178
Size: 30 165 30 924 30 4219
Fragments arranged by size:
4219 924 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 2546 1186
Size: 899 461 4038
Fragments arranged by size:
4038 899 461
BseYI C'CCAG_C
Cuts at: 2466 4601 5097 2466
Size: 2135 496 2767
Fragments arranged by size:
2767 2135 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4825
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2848 3609 4637 1540
Size: 275 186 847 761 1028 2301
Fragments arranged by size:
2301 1028 847 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3459 3544 4615 3459
Size: 85 1071 4242
Fragments arranged by size:
4242 1071 85
BsmI GAATG_Cn'
Cuts at: 2909 3002 2909
Size: 93 5305
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2345 2717 3459 3544 4615 1391
Size: 399 555 372 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 555 399 372 85
BspEI T'CCGG_A
Cuts at: 5061 5061
Size: 5398
BspHI T'CATG_A
Cuts at: 3287 5017 3287
Size: 1730 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2753 844
Size: 1909 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3285 1394
Size: 429 1462 3507
Fragments arranged by size:
3507 1462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3893 4067 65
Size: 1183 2645 174 1396
Fragments arranged by size:
2645 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4042 4229 1700
Size: 110 2232 187 2869
Fragments arranged by size:
2869 2232 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5398
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5398
BssSI C'ACGA_G
Cuts at: 3458 4470 3458
Size: 1012 4386
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5398
BstBI TT'CG_AA
Cuts at: 4202 4202
Size: 5398
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5398
BstYI r'GATC_y
Cuts at: 1972 2277 2728 3095 3480 3497 4949 5035 5047
Size: 305 451 367 385 17 1452 86 12
Cuts at: 5047 5393 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 451 385 367 346 305 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4846
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4659
Fragments arranged by size:
4659 683 56
BtsI GCAGTG_nn'
Cuts at: 2914 3708 3728 2914
Size: 794 20 4584
Fragments arranged by size:
4584 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5363
DraI TTT'AAA
Cuts at: 2702 2702
Size: 5398
DraIII CAC_nnn'GTG
Cuts at: 2207 2207
Size: 5398
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4405 809
Size: 507 3089 1802
Fragments arranged by size:
3089 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2845 3734 5363
Size: 54 1425 50 275 18 1015 889 1629
Cuts at: 5363 8
Size: 43
Fragments arranged by size:
1629 1425 1015 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2845 1537
Size: 275 1033 4090
Fragments arranged by size:
4090 1033 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 3328 1353
Size: 674 1301 3423
Fragments arranged by size:
3423 1301 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3985 4359 4505 387
Size: 1064 138 2396 374 146 1280
Fragments arranged by size:
2396 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2119 2619 3461 4058 4845 5113
Size: 393 412 75 500 842 597 787 268
Cuts at: 5113 1239
Size: 1524
Fragments arranged by size:
1524 842 787 597 500 412 393 268
75
EcoO109I rG'GnC_Cy
Cuts at: 2148 2186 2148
Size: 38 5360
EcoRI G'AATT_C
Cuts at: 2707 3085 2707
Size: 378 5020
FspI TGC'GCA
Cuts at: 3904 3904
Size: 5398
HaeII r_GCGC'y
Cuts at: 1678 2539 4545 1678
Size: 861 2006 2531
Fragments arranged by size:
2531 2006 861
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 2606 2638 4039
Size: 32 135 32 755 32 247 32 1401
Cuts at: 4039 4071 4113 4145 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1401 755 247 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2742 2988 669
Size: 988 281 804 246 3079
Fragments arranged by size:
3079 988 804 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5398
HpaI GTT'AAC
Cuts at: 2988 2988
Size: 5398
KpnI G_GTAC'C
Cuts at: 2723 2723
Size: 5398
MfeI C'AATT_G
Cuts at: 2997 2997
Size: 5398
MluI A'CGCG_T
Cuts at: 5274 5274
Size: 5398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4512 4696 1305
Size: 3207 184 2007
Fragments arranged by size:
3207 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3576
Fragments arranged by size:
3576 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3356 3715 3874 518
Size: 648 1329 861 359 159 2042
Fragments arranged by size:
2042 1329 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2814 3491 4639 4884 1606
Size: 1208 677 1148 245 2120
Fragments arranged by size:
2120 1208 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4846
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5398
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5398
NotI GC'GGCC_GC
Cuts at: 2845 2845
Size: 5398
NspI r_CATG'y
Cuts at: 1132 1229 2756 4301 1132
Size: 97 1527 1545 2229
Fragments arranged by size:
2229 1545 1527 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5398
PciI A'CATG_T
Cuts at: 4297 4297
Size: 5398
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5398
PfoI T'CCnGG_A
Cuts at: 2044 2661 2044
Size: 617 4781
PmeI GTTT'AAAC
Cuts at: 2702 2702
Size: 5398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3499 4980 5012 3467
Size: 32 1481 32 3853
Fragments arranged by size:
3853 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2186 2186
Size: 5398
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5398
PsiI TTA'TAA
Cuts at: 2968 2968
Size: 5398
PstI C_TGCA'G
Cuts at: 830 2750 830
Size: 1920 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5398
SalI G'TCGA_C
Cuts at: 1936 2740 1936
Size: 804 4594
SbfI CC_TGCA'GG
Cuts at: 2750 2750
Size: 5398
ScaI AGT'ACT
Cuts at: 3646 5358 3646
Size: 1712 3686
SexAI A'CCwGG_T
Cuts at: 2203 2203
Size: 5398
SfcI C'TryA_G
Cuts at: 826 1045 2459 2574 2746 3881 4562 4753 826
Size: 219 1414 115 172 1135 681 191 1471
Fragments arranged by size:
1471 1414 1135 681 219 191 172 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5398
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5398
SmaI CCC'GGG
Cuts at: 1872 2696 2725 1872
Size: 824 29 4545
Fragments arranged by size:
4545 824 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 3502 4403 4665 820
Size: 197 926 306 1253 901 262 1553
Fragments arranged by size:
1553 1253 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 3189 493
Size: 2696 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5398
SphI G_CATG'C
Cuts at: 1132 2756 1132
Size: 1624 3774
SspI AAT'ATT
Cuts at: 5 52 3322 5
Size: 47 3270 2081
Fragments arranged by size:
3270 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5398
StyI C'CwwG_G
Cuts at: 513 1065 2606 2837 513
Size: 552 1541 231 3074
Fragments arranged by size:
3074 1541 552 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3425 3442 3595 3780 4225 325
Size: 1255 1845 17 153 185 445 1498
Fragments arranged by size:
1845 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3644 5356 96
Size: 274 80 33 51 1443 1667 1712 138
Fragments arranged by size:
1712 1667 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5358 5390 5358
Size: 32 5366
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5398
XbaI T'CTAG_A
Cuts at: 2734 2734
Size: 5398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2334 2444 2334
Size: 110 5288
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5398
XmaI C'CCGG_G
Cuts at: 1870 2694 2723 1870
Size: 824 29 4545
Fragments arranged by size:
4545 824 29
XmnI GAAnn'nnTTC
Cuts at: 3527 3527
Size: 5398
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3206 276
Size: 53 83 186 936 1672 2468
Fragments arranged by size:
2468 1672 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI