Restriction map for
pFN21AB6327
(Circular) (Six-base) MAPSORT of: pFN21AB6327.seq Check: 4301 from: 1 to: 5488
pFN21AB6327 5488 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2622 2809 2622
Size: 187 5301
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3551 4935 5203 1632
Size: 1919 1384 268 1917
Fragments arranged by size:
1919 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 2195 1166
Size: 1029 4459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 3231 4803 3231
Size: 1572 3916
ApaI G_GGCC'C
Cuts at: 2402 2402
Size: 5488
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 2813 1870
Size: 73 375 495 4545
Fragments arranged by size:
4545 495 375 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2622 2809 4264 618
Size: 325 217 627 835 187 1455 1842
Fragments arranged by size:
1842 1455 835 627 325 217 187
BanII G_rGCy'C
Cuts at: 1391 2244 2325 2402 2807 1391
Size: 853 81 77 405 4072
Fragments arranged by size:
4072 853 405 81 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2345 928
Size: 971 446 4071
Fragments arranged by size:
4071 971 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2052 3380 4590 2052
Size: 1328 1210 2950
Fragments arranged by size:
2950 1328 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2397 4099 136
Size: 107 122 71 631 605 725 1702 1525
Fragments arranged by size:
1702 1525 725 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 2402 2509 3549 4705 1790
Size: 612 107 1040 1156 2573
Fragments arranged by size:
2573 1156 1040 612 107
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 2573 3219
Size: 416 53 83 399 24 1122 34 646
Cuts at: 3219 4177 442
Size: 958 1753
Fragments arranged by size:
1753 1122 958 646 416 399 83 53
34 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2309 2492 4148 1239
Size: 989 81 183 1656 2579
Fragments arranged by size:
2579 1656 989 183 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 3613 4478 4776 2210
Size: 1403 865 298 2922
Fragments arranged by size:
2922 1403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2096 2671 4151 882
Size: 617 597 575 1480 2219
Fragments arranged by size:
2219 1480 617 597 575
BsaAI yAC'GTr
Cuts at: 493 1204 2776 3279 493
Size: 711 1572 503 2702
Fragments arranged by size:
2702 1572 711 503
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 3677 4312 275
Size: 53 83 186 936 1762 382 635 1451
Fragments arranged by size:
1762 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2651 2681 1178
Size: 30 165 30 1248 30 3985
Fragments arranged by size:
3985 1248 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2390 1186
Size: 1096 108 4284
Fragments arranged by size:
4284 1096 108
BseYI C'CCAG_C
Cuts at: 2526 4691 5187 2526
Size: 2165 496 2827
Fragments arranged by size:
2827 2165 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2720 1163
Size: 573 984 3931
Fragments arranged by size:
3931 984 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2325 3549 3634 4705 2244
Size: 81 1224 85 1071 3027
Fragments arranged by size:
3027 1224 1071 85 81
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2325 2402 2509 2807 3549 3634
Size: 399 454 81 77 107 298 742 85
Cuts at: 3634 4705 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 298 107 85
81 77
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2114 2843 844
Size: 1270 729 3489
Fragments arranged by size:
3489 1270 729
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2095 3983 4157 65
Size: 1183 847 1888 174 1396
Fragments arranged by size:
1888 1396 1183 847 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2460 2536 2744 2764 4132 4319 1700
Size: 110 650 76 208 20 1368 187 2869
Fragments arranged by size:
2869 1368 650 208 187 110 76 20
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 3548 4560 3548
Size: 1012 4476
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstXI CCAn_nnnn'nTGG
Cuts at: 2552 2776 2552
Size: 224 5264
BstYI r'GATC_y
Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483
Size: 846 367 385 17 1452 86 12 346
Cuts at: 5483 1972
Size: 1977
Fragments arranged by size:
1977 1452 846 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2046 2193 513
Size: 552 981 147 3808
Fragments arranged by size:
3808 981 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3798 3818 3004
Size: 794 20 4674
Fragments arranged by size:
4674 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2690 4495 809
Size: 507 1374 1805 1802
Fragments arranged by size:
1805 1802 1374 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2766 2935 3824
Size: 54 1425 50 275 18 936 169 889
Cuts at: 3824 5453 8
Size: 1629 43
Fragments arranged by size:
1629 1425 936 889 275 169 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 3418 1353
Size: 1028 1037 3423
Fragments arranged by size:
3423 1037 1028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2216 4075 4449 4595 387
Size: 1064 138 627 1859 374 146 1280
Fragments arranged by size:
1859 1280 1064 627 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 2323 2242
Size: 81 5407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2309 2492 3551 4148 4935 5203
Size: 393 596 81 183 1059 597 787 268
Cuts at: 5203 1239
Size: 1524
Fragments arranged by size:
1524 1059 787 597 596 393 268 183
81
EcoO109I rG'GnC_Cy
Cuts at: 2399 2399
Size: 5488
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 5488
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 2601 2649 4635 1678
Size: 923 48 1986 2531
Fragments arranged by size:
2531 1986 923 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2651 2683 4129
Size: 32 135 32 454 32 593 32 1446
Cuts at: 4129 4161 4203 4235 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1446 593 454 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2695 2832 3078 669
Size: 988 281 757 137 246 3079
Fragments arranged by size:
3079 988 757 281 246 137
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2626 2813 2626
Size: 187 5301
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 2756 3446 3805 3964 518
Size: 648 1029 561 690 359 159 2042
Fragments arranged by size:
2042 1029 690 648 561 359 159
MspA1I CmG'CkG
Cuts at: 1606 2904 3581 4729 4974 1606
Size: 1298 677 1148 245 2120
Fragments arranged by size:
2120 1298 1148 677 245
NaeI GCC'GGC
Cuts at: 2766 2766
Size: 5488
NcoI C'CATG_G
Cuts at: 513 1065 2193 513
Size: 552 1128 3808
Fragments arranged by size:
3808 1128 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NgoMIV G'CCGG_C
Cuts at: 2764 2764
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2091 2846 4391 1132
Size: 97 862 755 1545 2229
Fragments arranged by size:
2229 1545 862 755 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2609 1108
Size: 1501 3987
PfoI T'CCnGG_A
Cuts at: 2673 2673
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PspOMI G'GGCC_C
Cuts at: 2398 2398
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2575 2840 830
Size: 1745 265 3478
Fragments arranged by size:
3478 1745 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
SacI G_AGCT'C
Cuts at: 2244 2325 2244
Size: 81 5407
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SapI GCTCTTCn'nnn_
Cuts at: 2381 2381
Size: 5488
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2571 2641 2836 3971 4652 4843 826
Size: 219 1526 70 195 1135 681 191 1471
Fragments arranged by size:
1526 1471 1135 681 219 195 191 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 2744 1700
Size: 1044 4444
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 3592 4493 4755 820
Size: 197 926 282 1367 901 262 1553
Fragments arranged by size:
1553 1367 926 901 282 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2091 2846 1132
Size: 959 755 3774
Fragments arranged by size:
3774 959 755
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5488
StyI C'CwwG_G
Cuts at: 513 1065 2193 2927 513
Size: 552 1128 734 3074
Fragments arranged by size:
3074 1128 734 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3532 3685 3870 4315 325
Size: 1255 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3734 5446 96
Size: 274 80 33 51 1443 1757 1712 138
Fragments arranged by size:
1757 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 2663 1316
Size: 1347 4141
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2552 2552
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 2217 3617 2217
Size: 1400 4088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI