Restriction map for
pFN21AB6326
(Circular) (Six-base) MAPSORT of: pFN21AB6326.seq Check: 179 from: 1 to: 5563
pFN21AB6326 5563 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2906 1937
Size: 969 4594
Acc65I G'GTAC_C
Cuts at: 2884 2884
Size: 5563
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 3626 5010 5278 1632
Size: 624 1370 1384 268 1917
Fragments arranged by size:
1917 1384 1370 624 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 4462 5439 1203
Size: 3259 977 1327
Fragments arranged by size:
3259 1327 977
AgeI A'CCGG_T
Cuts at: 4394 4394
Size: 5563
AhdI GACnn_n'nnGTC
Cuts at: 4292 4292
Size: 5563
AleI CACnn'nnGTG
Cuts at: 1166 2438 1166
Size: 1272 4291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5563
AlwNI CAG_nnn'CTG
Cuts at: 3306 4878 3306
Size: 1572 3991
ApaLI G'TGCA_C
Cuts at: 3620 4776 3620
Size: 1156 4407
ApoI r'AATT_y
Cuts at: 1493 2410 2872 3098 3250 5359 1493
Size: 917 462 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 462 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4117 160
Size: 872 3085 1606
Fragments arranged by size:
3085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2888 1870
Size: 73 945 4545
Fragments arranged by size:
4545 945 73
BamHI G'GATC_C
Cuts at: 2893 3260 2893
Size: 367 5196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2884 4339 618
Size: 325 217 627 1097 1455 1842
Fragments arranged by size:
1842 1455 1097 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2493 2882 1391
Size: 1102 389 4072
Fragments arranged by size:
4072 1102 389
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2563 928
Size: 971 664 3928
Fragments arranged by size:
3928 971 664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3754 3788 1903
Size: 34 1817 34 3678
Fragments arranged by size:
3678 1817 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 4665 3455
Size: 1210 4353
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2082 4174 136
Size: 107 122 71 631 605 410 2092 1525
Fragments arranged by size:
2092 1525 631 605 410 122 107 71
BglII A'GATC_T
Cuts at: 2104 5558 2104
Size: 3454 2109
BlpI GC'TnA_GC
Cuts at: 2980 2980
Size: 5563
Bme1580I G_kGCm'C
Cuts at: 1790 2836 3624 4780 1790
Size: 1046 788 1156 2573
Fragments arranged by size:
2573 1156 1046 788
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3294 4252 442
Size: 416 53 83 399 24 1877 958 1753
Fragments arranged by size:
1877 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4223 1239
Size: 2984 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4553 4851 3688
Size: 865 298 4400
Fragments arranged by size:
4400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2704 4226 882
Size: 617 1205 1522 2219
Fragments arranged by size:
2219 1522 1205 617
BsaAI yAC'GTr
Cuts at: 493 1204 3354 493
Size: 711 2150 2702
Fragments arranged by size:
2702 2150 711
BsaBI GATnn'nnATC
Cuts at: 5557 5557
Size: 5563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3370 3752 4387 275
Size: 53 83 186 936 1837 382 635 1451
Fragments arranged by size:
1837 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3996 4394 4668 4815 5226 3996
Size: 398 274 147 411 4333
Fragments arranged by size:
4333 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5338
Fragments arranged by size:
5338 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2003 1186
Size: 817 4746
BseYI C'CCAG_C
Cuts at: 4766 5262 4766
Size: 496 5067
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2271 1163
Size: 573 535 4455
Fragments arranged by size:
4455 573 535
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3774 4802 1540
Size: 275 186 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3624 3709 4780 3624
Size: 85 1071 4407
Fragments arranged by size:
4407 1071 85
BsmI GAATG_Cn'
Cuts at: 3074 3167 3074
Size: 93 5470
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 2836 2882 3624 3709 4780 1391
Size: 399 703 343 46 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 703 399 343 85 46
BspEI T'CCGG_A
Cuts at: 5226 5226
Size: 5563
BspHI T'CATG_A
Cuts at: 3452 5182 3452
Size: 1730 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2918 844
Size: 2074 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3450 1394
Size: 429 1627 3507
Fragments arranged by size:
3507 1627 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2557 4058 4232 65
Size: 1183 1309 1501 174 1396
Fragments arranged by size:
1501 1396 1309 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4207 4394 1700
Size: 110 2397 187 2869
Fragments arranged by size:
2869 2397 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5563
BssSI C'ACGA_G
Cuts at: 2042 3623 4635 2042
Size: 1581 1012 2970
Fragments arranged by size:
2970 1581 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5563
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5563
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2194
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2104 2852 2893 3260 3645 3662 5114 5200
Size: 132 748 41 367 385 17 1452 86
Cuts at: 5200 5212 5558 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 748 385 367 346 132 86
41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2385 2775 2385
Size: 390 5173
BtgI C'CryG_G
Cuts at: 513 1065 2493 513
Size: 552 1428 3583
Fragments arranged by size:
3583 1428 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4824
Fragments arranged by size:
4824 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 3079 3873 3893 2100
Size: 979 794 20 3770
Fragments arranged by size:
3770 979 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5528
DraI TTT'AAA
Cuts at: 2867 2867
Size: 5563
DraIII CAC_nnn'GTG
Cuts at: 2249 2249
Size: 5563
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4570 809
Size: 507 3254 1802
Fragments arranged by size:
3254 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3010 3899 5528
Size: 54 1425 50 275 18 1180 889 1629
Cuts at: 5528 8
Size: 43
Fragments arranged by size:
1629 1425 1180 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2427 2834 3493 1353
Size: 1074 407 659 3423
Fragments arranged by size:
3423 1074 659 407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2514 4150 4524 4670 387
Size: 1064 138 925 1636 374 146 1280
Fragments arranged by size:
1636 1280 1064 925 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 3626 4223 5010 5278 1239
Size: 393 624 1370 597 787 268 1524
Fragments arranged by size:
1524 1370 787 624 597 393 268
EcoRI G'AATT_C
Cuts at: 2872 3250 2872
Size: 378 5185
FspI TGC'GCA
Cuts at: 4069 4069
Size: 5563
HaeII r_GCGC'y
Cuts at: 1678 4710 1678
Size: 3032 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2610 2642 4204 4236 4278
Size: 32 135 32 1038 32 1562 32 42
Cuts at: 4278 4310 1373
Size: 32 2626
Fragments arranged by size:
2626 1562 1038 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2758 2907 3153 669
Size: 988 281 820 149 246 3079
Fragments arranged by size:
3079 988 820 281 246 149
HindIII A'AGCT_T
Cuts at: 748 2238 2802 748
Size: 1490 564 3509
Fragments arranged by size:
3509 1490 564
HpaI GTT'AAC
Cuts at: 3153 3153
Size: 5563
KpnI G_GTAC'C
Cuts at: 2888 2888
Size: 5563
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 5439 5439
Size: 5563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2747 4677 4861 1305
Size: 1442 1930 184 2007
Fragments arranged by size:
2007 1930 1442 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3741
Fragments arranged by size:
3741 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 3521 3880 4039 518
Size: 648 1272 1083 359 159 2042
Fragments arranged by size:
2042 1272 1083 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2979 3656 4804 5049 1606
Size: 1373 677 1148 245 2120
Fragments arranged by size:
2120 1373 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2493 513
Size: 552 1428 3583
Fragments arranged by size:
3583 1428 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5563
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2921 4466 1132
Size: 97 1692 1545 2229
Fragments arranged by size:
2229 1692 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5563
PciI A'CATG_T
Cuts at: 4462 4462
Size: 5563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5563
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 5145 5177 3632
Size: 32 1481 32 4018
Fragments arranged by size:
4018 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5563
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PstI C_TGCA'G
Cuts at: 830 2488 2915 830
Size: 1658 427 3478
Fragments arranged by size:
3478 1658 427
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SapI GCTCTTCn'nnn_
Cuts at: 2427 2427
Size: 5563
SbfI CC_TGCA'GG
Cuts at: 2915 2915
Size: 5563
ScaI AGT'ACT
Cuts at: 2471 3811 5523 2471
Size: 1340 1712 2511
Fragments arranged by size:
2511 1712 1340
SfcI C'TryA_G
Cuts at: 826 1045 2436 2484 2911 4046 4727 4918 826
Size: 219 1391 48 427 1135 681 191 1471
Fragments arranged by size:
1471 1391 1135 681 427 219 191 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2890 1872
Size: 1018 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3667 4568 4830 820
Size: 197 926 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3354 493
Size: 2861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2921 1132
Size: 1789 3774
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5563
StyI C'CwwG_G
Cuts at: 513 1065 2493 3002 513
Size: 552 1428 509 3074
Fragments arranged by size:
3074 1428 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3590 3607 3760 3945 4390 325
Size: 1255 2010 17 153 185 445 1498
Fragments arranged by size:
2010 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 3809 5521
Size: 274 80 33 51 1443 492 1340 1712
Cuts at: 5521 96
Size: 138
Fragments arranged by size:
1712 1443 1340 492 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2407 2439 5523 5555 2407
Size: 32 3084 32 2415
Fragments arranged by size:
3084 2415 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5563
XbaI T'CTAG_A
Cuts at: 2696 2899 2696
Size: 203 5360
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5563
XmaI C'CCGG_G
Cuts at: 1870 2888 1870
Size: 1018 4545
XmnI GAAnn'nnTTC
Cuts at: 2286 3692 2286
Size: 1406 4157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI