Restriction map for
pFN21AB6318
(Circular) (Six-base) MAPSORT of: pFN21AB6318.seq Check: 1634 from: 1 to: 5548
pFN21AB6318 5548 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 2468 3611 4995 5263 1632
Size: 651 185 1143 1384 268 1917
Fragments arranged by size:
1917 1384 1143 651 268 185
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AlwNI CAG_nnn'CTG
Cuts at: 2741 3291 4863 2741
Size: 550 1572 3426
Fragments arranged by size:
3426 1572 550
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2442 2518 2857 3083 3235 5344 1493
Size: 949 76 339 226 152 2109 1697
Fragments arranged by size:
2109 1697 949 339 226 152 76
AseI AT'TA_AT
Cuts at: 160 1032 2274 2585 2835 4102 160
Size: 872 1242 311 250 1267 1606
Fragments arranged by size:
1606 1267 1242 872 311 250
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2100 2183 2873 1870
Size: 73 157 83 690 4545
Fragments arranged by size:
4545 690 157 83 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2869 4324 618
Size: 325 217 627 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2058 2217 2867 1391
Size: 667 159 650 4072
Fragments arranged by size:
4072 667 650 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 928
Size: 971 213 4364
Fragments arranged by size:
4364 971 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2400 2823 3440 4650 2400
Size: 423 617 1210 3298
Fragments arranged by size:
3298 1210 617 423
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4159 136
Size: 107 122 71 631 605 2487 1525
Fragments arranged by size:
2487 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5543 5543
Size: 5548
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 3609 4765 1790
Size: 1819 1156 2573
Fragments arranged by size:
2573 1819 1156
BmgBI CAC'GTC
Cuts at: 1318 2764 1318
Size: 1446 4102
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 4208 1239
Size: 1388 1581 2579
Fragments arranged by size:
2579 1581 1388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3673 4538 4836 3673
Size: 865 298 4385
Fragments arranged by size:
4385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2092 4211 882
Size: 617 593 2119 2219
Fragments arranged by size:
2219 2119 617 593
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3355 3737 4372 275
Size: 53 83 186 936 1822 382 635 1451
Fragments arranged by size:
1822 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 1186
Size: 1030 4518
BseYI C'CCAG_C
Cuts at: 4751 5247 4751
Size: 496 5052
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2107 1163
Size: 573 371 4604
Fragments arranged by size:
4604 573 371
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2167 2998 3759 4787 1540
Size: 275 186 166 831 761 1028 2301
Fragments arranged by size:
2301 1028 831 761 275 186 166
BsiHKAI G_wGCw'C
Cuts at: 2058 3609 3694 4765 2058
Size: 1551 85 1071 2841
Fragments arranged by size:
2841 1551 1071 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2217 2867 3609 3694 4765 1391
Size: 399 268 159 650 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 650 399 268 159 85
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 844
Size: 2059 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2219 3435 1394
Size: 429 396 1216 3507
Fragments arranged by size:
3507 1216 429 396
BsrDI GCAATG_nn'
Cuts at: 65 1248 2816 4043 4217 65
Size: 1183 1568 1227 174 1396
Fragments arranged by size:
1568 1396 1227 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4192 4379 1700
Size: 110 2382 187 2869
Fragments arranged by size:
2869 2382 187 110
BsrGI T'GTAC_A
Cuts at: 96 2467 96
Size: 2371 3177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2400 2878 3245 3630 3647 5099 5185 5197
Size: 428 478 367 385 17 1452 86 12
Cuts at: 5197 5543 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 478 428 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2042 513
Size: 552 939 38 4019
Fragments arranged by size:
4019 939 552 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 3064 3858 3878 2240
Size: 824 794 20 3910
Fragments arranged by size:
3910 824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2164 2822 2995
Size: 54 1425 50 275 18 334 658 173
Cuts at: 2995 3884 5513 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 658 334 275 173 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2164 2995 1537
Size: 275 352 831 4090
Fragments arranged by size:
4090 831 352 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3478 1353
Size: 2125 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2080 4135 4509 4655 387
Size: 1064 138 491 2055 374 146 1280
Fragments arranged by size:
2055 1280 1064 491 374 146 138
EcoICRI GAG'CTC
Cuts at: 2056 2056
Size: 5548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2468 2627 3611 4208 4995 5263
Size: 393 651 185 159 984 597 787 268
Cuts at: 5263 1239
Size: 1524
Fragments arranged by size:
1524 984 787 651 597 393 268 185
159
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FspI TGC'GCA
Cuts at: 2232 4054 2232
Size: 1822 3726
HaeII r_GCGC'y
Cuts at: 1678 4695 1678
Size: 3017 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 2295 748
Size: 1547 4001
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3506 3865 4024 518
Size: 648 2340 359 159 2042
Fragments arranged by size:
2340 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2252 2964 3641 4789 5034 1606
Size: 646 712 677 1148 245 2120
Fragments arranged by size:
2120 1148 712 677 646 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2042 513
Size: 552 939 38 4019
Fragments arranged by size:
4019 939 552 38
NdeI CA'TA_TG
Cuts at: 387 2511 387
Size: 2124 3424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2164 2995 2164
Size: 831 4717
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2083 1108
Size: 975 4573
PfoI T'CCnGG_A
Cuts at: 2403 2747 2403
Size: 344 5204
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 2711 3118 2711
Size: 407 5141
PstI C_TGCA'G
Cuts at: 830 2900 830
Size: 2070 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2252 2252
Size: 5548
SacI G_AGCT'C
Cuts at: 2058 2058
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 2334 3796 5508 2334
Size: 1462 1712 2374
Fragments arranged by size:
2374 1712 1462
SfcI C'TryA_G
Cuts at: 826 1045 2896 4031 4712 4903 826
Size: 219 1851 1135 681 191 1471
Fragments arranged by size:
1851 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3652 4553 4815 820
Size: 197 926 1709 901 262 1553
Fragments arranged by size:
1709 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 2081 1517
Size: 564 4984
StyI C'CwwG_G
Cuts at: 513 1065 2004 2042 2082 2987 513
Size: 552 939 38 40 905 3074
Fragments arranged by size:
3074 939 905 552 40 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 3575 3592 3745 3930 4375 325
Size: 1255 579 1416 17 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 579 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2332 2467 3794
Size: 274 80 33 51 1443 355 135 1327
Cuts at: 3794 5506 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1327 355 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2401
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 2109 3677 2109
Size: 1568 3980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI