Restriction map for
pFN21AB6316
(Circular) (Six-base) MAPSORT of: pFN21AB6316.seq Check: 6360 from: 1 to: 5548
pFN21AB6316 5548 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 3611 4995 5263 1632
Size: 449 1530 1384 268 1917
Fragments arranged by size:
1917 1530 1384 449 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AlwNI CAG_nnn'CTG
Cuts at: 3291 4863 3291
Size: 1572 3976
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2050 2220 2320 2857 3083 3235 5344 1493
Size: 557 170 100 537 226 152 2109 1697
Fragments arranged by size:
2109 1697 557 537 226 170 152 100
AseI AT'TA_AT
Cuts at: 160 1032 2391 4102 160
Size: 872 1359 1711 1606
Fragments arranged by size:
1711 1606 1359 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 2873 1870
Size: 73 118 812 4545
Fragments arranged by size:
4545 812 118 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2869 4324 618
Size: 325 217 627 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2867 1391
Size: 1476 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2242 2508 2634 928
Size: 971 343 266 126 3842
Fragments arranged by size:
3842 971 343 266 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4159 136
Size: 107 122 71 631 605 2487 1525
Fragments arranged by size:
2487 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2360 2539 2665 5543 2360
Size: 179 126 2878 2365
Fragments arranged by size:
2878 2365 179 126
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 3609 4765 1790
Size: 1819 1156 2573
Fragments arranged by size:
2573 1819 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4208 1239
Size: 2969 2579
Bpu10I CC'TnA_GC
Cuts at: 2016 2016
Size: 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2394 2564 2690 3673 4538 4836 2394
Size: 170 126 983 865 298 3106
Fragments arranged by size:
3106 983 865 298 170 126
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4211 882
Size: 617 2712 2219
Fragments arranged by size:
2712 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3355 3737 4372 275
Size: 53 83 186 936 1822 382 635 1451
Fragments arranged by size:
1822 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 1186
Size: 850 4698
BseYI C'CCAG_C
Cuts at: 2555 2681 4751 5247 2555
Size: 126 2070 496 2856
Fragments arranged by size:
2856 2070 496 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2270 1163
Size: 573 534 4441
Fragments arranged by size:
4441 573 534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2998 3759 4787 1540
Size: 275 186 997 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3609 3694 4765 3609
Size: 85 1071 4392
Fragments arranged by size:
4392 1071 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2867 3609 3694 4765 1391
Size: 399 1077 742 85 1071 2174
Fragments arranged by size:
2174 1077 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 844
Size: 2059 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4192 4379 1700
Size: 110 2382 187 2869
Fragments arranged by size:
2869 2382 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2360 2539 2665 2878 3245 3630 3647 5099
Size: 388 179 126 213 367 385 17 1452
Cuts at: 5099 5185 5197 5543 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 388 385 367 346 213 179
126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 3064 3858 3878 2444
Size: 620 794 20 4114
Fragments arranged by size:
4114 794 620 20
ClaI AT'CG_AT
Cuts at: 1554 2257 1554
Size: 703 4845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2176 2852 2176
Size: 676 4872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2995 3884 5513
Size: 54 1425 50 275 18 1165 889 1629
Cuts at: 5513 8
Size: 43
Fragments arranged by size:
1629 1425 1165 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 1537
Size: 275 1183 4090
Fragments arranged by size:
4090 1183 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2281 3478 1353
Size: 714 214 1197 3423
Fragments arranged by size:
3423 1197 714 214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 3611 4208 4995 5263 1239
Size: 393 449 1530 597 787 268 1524
Fragments arranged by size:
1530 1524 787 597 449 393 268
EcoRI G'AATT_C
Cuts at: 2220 2857 3235 2220
Size: 637 378 4533
Fragments arranged by size:
4533 637 378
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 4695 1678
Size: 3017 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2599 2631 4189 4221 4263
Size: 32 135 32 1027 32 1558 32 42
Cuts at: 4263 4295 1373
Size: 32 2626
Fragments arranged by size:
2626 1558 1027 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2566 2692 3506 3865 4024 518
Size: 648 1400 126 814 359 159 2042
Fragments arranged by size:
2042 1400 814 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2964 3641 4789 5034 1606
Size: 1358 677 1148 245 2120
Fragments arranged by size:
2120 1358 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4996
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2447 2906 4451 1132
Size: 97 1218 459 1545 2229
Fragments arranged by size:
2229 1545 1218 459 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2075 2107 2075
Size: 32 5516
PstI C_TGCA'G
Cuts at: 830 2466 2900 830
Size: 1636 434 3478
Fragments arranged by size:
3478 1636 434
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SapI GCTCTTCn'nnn_
Cuts at: 2281 2281
Size: 5548
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2332 2462 2896 4031 4712 4903 826
Size: 219 1287 130 434 1135 681 191 1471
Fragments arranged by size:
1471 1287 1135 681 434 219 191 130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2409 2543 2669 3652 4553 4815
Size: 197 926 466 134 126 983 901 262
Cuts at: 4815 820
Size: 1553
Fragments arranged by size:
1553 983 926 901 466 262 197 134
126
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 2417 3472 5
Size: 47 2365 1055 2081
Fragments arranged by size:
2365 2081 1055 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2987 513
Size: 552 1922 3074
Fragments arranged by size:
3074 1922 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2196 3575 3592 3745 3930 4375 325
Size: 1255 616 1379 17 153 185 445 1498
Fragments arranged by size:
1498 1379 1255 616 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3794 5506 96
Size: 274 80 33 51 1443 1817 1712 138
Fragments arranged by size:
1817 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 2076 2418 3677 2076
Size: 342 1259 3947
Fragments arranged by size:
3947 1259 342
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI