Restriction map for
pFN21AB6314
(Circular) (Six-base) MAPSORT of: pFN21AB6314.seq Check: 7622 from: 1 to: 5548
pFN21AB6314 5548 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2354 2589 2637 2891 1937
Size: 417 235 48 254 4594
Fragments arranged by size:
4594 417 254 235 48
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 3611 4995 5263 1632
Size: 605 1374 1384 268 1917
Fragments arranged by size:
1917 1384 1374 605 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2633 1184
Size: 1449 4099
AlwNI CAG_nnn'CTG
Cuts at: 2668 3291 4863 2668
Size: 623 1572 3353
Fragments arranged by size:
3353 1572 623
ApaI G_GGCC'C
Cuts at: 2077 2725 2077
Size: 648 4900
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2873 1870
Size: 73 930 4545
Fragments arranged by size:
4545 930 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2869 4324 618
Size: 325 217 627 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2077 2725 2796 2867 1391
Size: 686 648 71 71 4072
Fragments arranged by size:
4072 686 648 71 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2499 928
Size: 971 600 3977
Fragments arranged by size:
3977 971 600
BbvCI CC'TCA_GC
Cuts at: 2432 2432
Size: 5548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3739 3773 1903
Size: 34 50 34 1718 34 3678
Fragments arranged by size:
3678 1718 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2667 3440 4650 2667
Size: 773 1210 3565
Fragments arranged by size:
3565 1210 773
BclI T'GATC_A
Cuts at: 1548 2550 2767 1548
Size: 1002 217 4329
Fragments arranged by size:
4329 1002 217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2119 4159 136
Size: 107 122 71 631 605 447 2040 1525
Fragments arranged by size:
2040 1525 631 605 447 122 107 71
BglII A'GATC_T
Cuts at: 2037 5543 2037
Size: 3506 2042
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2115 2725 3609 4765 1790
Size: 287 38 610 884 1156 2573
Fragments arranged by size:
2573 1156 884 610 287 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 2400 2571
Size: 416 53 83 399 24 636 347 171
Cuts at: 2571 2718 3279 4237 442
Size: 147 561 958 1753
Fragments arranged by size:
1753 958 636 561 416 399 347 171
147 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 2541 4208 1239
Size: 825 477 1667 2579
Fragments arranged by size:
2579 1667 825 477
Bpu10I CC'TnA_GC
Cuts at: 2432 2624 2432
Size: 192 5356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2452 3673 4538 4836 2452
Size: 1221 865 298 3164
Fragments arranged by size:
3164 1221 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4211 882
Size: 617 2712 2219
Fragments arranged by size:
2712 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3355 3737 4372 275
Size: 53 83 186 936 1822 382 635 1451
Fragments arranged by size:
1822 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5548
BseYI C'CCAG_C
Cuts at: 4751 5247 4751
Size: 496 5052
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2686 1163
Size: 573 950 4025
Fragments arranged by size:
4025 950 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2998 3759 4787 1540
Size: 275 186 997 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2796 3609 3694 4765 2796
Size: 813 85 1071 3579
Fragments arranged by size:
3579 1071 813 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2115 2725 2796 2867 3609 3694
Size: 399 287 38 610 71 71 742 85
Cuts at: 3694 4765 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 610 399 287 85 71
71 38
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2625 2903 844
Size: 1781 278 3489
Fragments arranged by size:
3489 1781 278
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4192 4379 1700
Size: 110 2382 187 2869
Fragments arranged by size:
2869 2382 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstXI CCAn_nnnn'nTGG
Cuts at: 2482 2482
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2037 2665 2878 3245 3630 3647 5099 5185
Size: 65 628 213 367 385 17 1452 86
Cuts at: 5185 5197 5543 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 628 385 367 346 213 86
65 17 12
BstZ17I GTA'TAC
Cuts at: 2590 2590
Size: 5548
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 2062 2189 2751 3064 3858 3878 2062
Size: 127 562 313 794 20 3732
Fragments arranged by size:
3732 794 562 313 127 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2995 3884 5513
Size: 54 1425 50 275 18 1165 889 1629
Cuts at: 5513 8
Size: 43
Fragments arranged by size:
1629 1425 1165 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 1537
Size: 275 1183 4090
Fragments arranged by size:
4090 1183 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2321 3478 1353
Size: 863 105 1157 3423
Fragments arranged by size:
3423 1157 863 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2163 4135 4509 4655 387
Size: 1064 138 574 1972 374 146 1280
Fragments arranged by size:
1972 1280 1064 574 374 146 138
EcoICRI GAG'CTC
Cuts at: 2794 2794
Size: 5548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2237 2541 3611 4208 4995 5263
Size: 393 432 173 304 1070 597 787 268
Cuts at: 5263 1239
Size: 1524
Fragments arranged by size:
1524 1070 787 597 432 393 304 268
173
EcoNI CCTnn'n_nnAGG
Cuts at: 2327 2327
Size: 5548
EcoO109I rG'GnC_Cy
Cuts at: 2074 2074
Size: 5548
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 4695 1678
Size: 3017 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2782 2892 3138 669
Size: 988 281 844 110 246 3079
Fragments arranged by size:
3079 988 844 281 246 110
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2126 2487 4662 4846 1305
Size: 821 361 2175 184 2007
Fragments arranged by size:
2175 2007 821 361 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2775 3506 3865 4024 518
Size: 648 1609 731 359 159 2042
Fragments arranged by size:
2042 1609 731 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 2964 3641 4789 5034 1606
Size: 600 758 677 1148 245 2120
Fragments arranged by size:
2120 1148 758 677 600 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4996
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2070 1348
Size: 722 4826
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2668 1108
Size: 1560 3988
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PspOMI G'GGCC_C
Cuts at: 2073 2721 2073
Size: 648 4900
PstI C_TGCA'G
Cuts at: 830 2900 830
Size: 2070 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
SacI G_AGCT'C
Cuts at: 2796 2796
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SapI GCTCTTCn'nnn_
Cuts at: 2321 2321
Size: 5548
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2324 2896 4031 4712 4903 826
Size: 219 1279 572 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 681 572 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2467 3652 4553 4815 820
Size: 197 926 524 1185 901 262 1553
Fragments arranged by size:
1553 1185 926 901 524 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 2122 2244 1517
Size: 605 122 4821
Fragments arranged by size:
4821 605 122
StyI C'CwwG_G
Cuts at: 513 1065 2283 2987 513
Size: 552 1218 704 3074
Fragments arranged by size:
3074 1218 704 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2035 3575 3592 3745 3930 4375 325
Size: 1255 455 1540 17 153 185 445 1498
Fragments arranged by size:
1540 1498 1255 455 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2568 3794 5506
Size: 274 80 33 51 1443 591 1226 1712
Cuts at: 5506 96
Size: 138
Fragments arranged by size:
1712 1443 1226 591 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2077 2109 5508 5540 2077
Size: 32 3399 32 2085
Fragments arranged by size:
3399 2085 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI