Restriction map for
pFN21AB6309
(Circular) (Six-base) MAPSORT of: pFN21AB6309.seq Check: 5735 from: 1 to: 5479
pFN21AB6309 5479 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2477 2477
Size: 5479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 3542 4926 5194 1632
Size: 1028 882 1384 268 1917
Fragments arranged by size:
1917 1384 1028 882 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 4378 5355 1203
Size: 3175 977 1327
Fragments arranged by size:
3175 1327 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2116 1184
Size: 932 4547
AlwNI CAG_nnn'CTG
Cuts at: 2056 3222 4794 2056
Size: 1166 1572 2741
Fragments arranged by size:
2741 1572 1166
ApaI G_GGCC'C
Cuts at: 2116 2456 2116
Size: 340 5139
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2041 2348 2804 1870
Size: 73 77 21 307 456 4545
Fragments arranged by size:
4545 456 307 77 73 21
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2800 4255 618
Size: 325 217 627 1013 1455 1842
Fragments arranged by size:
1842 1455 1013 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2116 2456 2798 1391
Size: 725 340 342 4072
Fragments arranged by size:
4072 725 342 340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4508
BbvCI CC'TCA_GC
Cuts at: 2373 2373
Size: 5479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3670 3704 1903
Size: 34 1733 34 3678
Fragments arranged by size:
3678 1733 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 2334 1548
Size: 786 4693
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2142 4090
Size: 107 122 71 631 605 343 127 1948
Cuts at: 4090 136
Size: 1525
Fragments arranged by size:
1948 1525 631 605 343 127 122 107
71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2212 2896 2212
Size: 684 4795
Bme1580I G_kGCm'C
Cuts at: 1790 2116 2256 2456 2757 3540 4696 1790
Size: 326 140 200 301 783 1156 2573
Fragments arranged by size:
2573 1156 783 326 301 200 140
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2072 2168 2211 1056
Size: 1016 96 43 4324
Fragments arranged by size:
4324 1016 96 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2290 2637 4139 1239
Size: 1051 347 1502 2579
Fragments arranged by size:
2579 1502 1051 347
Bpu10I CC'TnA_GC
Cuts at: 2373 2373
Size: 5479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4142 882
Size: 617 2643 2219
Fragments arranged by size:
2643 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2277 3270 493
Size: 711 1073 993 2702
Fragments arranged by size:
2702 1073 993 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3286 3668 4303 275
Size: 53 83 186 936 1753 382 635 1451
Fragments arranged by size:
1753 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2289 3912 4310 4584 4731 5142 2289
Size: 1623 398 274 147 411 2626
Fragments arranged by size:
2626 1623 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5254
Fragments arranged by size:
5254 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 1186
Size: 1192 4287
BseYI C'CCAG_C
Cuts at: 2056 2085 2227 4682 5178 2056
Size: 29 142 2455 496 2357
Fragments arranged by size:
2455 2357 496 142 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4906
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2736 2929 3690 4718 1540
Size: 275 186 735 193 761 1028 2301
Fragments arranged by size:
2301 1028 761 735 275 193 186
BsiHKAI G_wGCw'C
Cuts at: 2276 3540 3625 4696 2276
Size: 1264 85 1071 3059
Fragments arranged by size:
3059 1264 1071 85
BsiWI C'GTAC_G
Cuts at: 2277 2277
Size: 5479
BsmI GAATG_Cn'
Cuts at: 2402 2590 2767 2990 3083 2402
Size: 188 177 223 93 4798
Fragments arranged by size:
4798 223 188 177 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2161 2161
Size: 5479
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2256 2276 2456 2757 2798 3540
Size: 399 326 140 20 180 301 41 742
Cuts at: 3540 3625 4696 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 326 301 180 140
85 41 20
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 2477 2834 844
Size: 1598 35 357 3489
Fragments arranged by size:
3489 1598 357 35
BsrBI CCG'CTC
Cuts at: 1394 1823 2506 3366 1394
Size: 429 683 860 3507
Fragments arranged by size:
3507 860 683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 4123 4310 1700
Size: 110 204 2109 187 2869
Fragments arranged by size:
2869 2109 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 2089 2216 1925
Size: 164 127 5188
Fragments arranged by size:
5188 164 127
BssSI C'ACGA_G
Cuts at: 2249 3539 4551 2249
Size: 1290 1012 3177
Fragments arranged by size:
3177 1290 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3988
Fragments arranged by size:
3988 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3789 3809 2995
Size: 794 20 4665
Fragments arranged by size:
4665 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DraIII CAC_nnn'GTG
Cuts at: 2558 2558
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2733 2926
Size: 54 1425 50 275 18 177 726 193
Cuts at: 2926 3815 5444 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 726 275 193 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2733 2926 1537
Size: 275 921 193 4090
Fragments arranged by size:
4090 921 275 193
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 3409 1353
Size: 1130 926 3423
Fragments arranged by size:
3423 1130 926
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 2637 2660 3542 4139 4926 5194
Size: 393 658 347 23 882 597 787 268
Cuts at: 5194 1239
Size: 1524
Fragments arranged by size:
1524 882 787 658 597 393 347 268
23
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 2498
Size: 5479
EcoO109I rG'GnC_Cy
Cuts at: 2112 2453 2112
Size: 341 5138
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 2069 2248 4626 1678
Size: 391 179 2378 2531
Fragments arranged by size:
2531 2378 391 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2301 2333 2506 2538 4120
Size: 32 135 32 729 32 173 32 1582
Cuts at: 4120 4152 4194 4226 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1582 729 173 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4593 4777 1305
Size: 3288 184 2007
Fragments arranged by size:
3288 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2606 3437 3796 3955 518
Size: 648 867 573 831 359 159 2042
Fragments arranged by size:
2042 867 831 648 573 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2633 2674 2895 3572 4720 4965 1606
Size: 471 556 41 221 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 556 471 245 221 41
NaeI GCC'GGC
Cuts at: 2016 2016
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3988
Fragments arranged by size:
3988 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NgoMIV G'CCGG_C
Cuts at: 2014 2014
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 2068 2164 2207 1052
Size: 1016 96 43 4324
Fragments arranged by size:
4324 1016 96 43
NotI GC'GGCC_GC
Cuts at: 2733 2926 2733
Size: 193 5286
NspI r_CATG'y
Cuts at: 1132 1229 2100 2837 4382 1132
Size: 97 871 737 1545 2229
Fragments arranged by size:
2229 1545 871 737 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2056 1108
Size: 948 4531
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2112 2452 2112
Size: 340 5139
PstI C_TGCA'G
Cuts at: 830 2831 830
Size: 2001 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2077 2633 2077
Size: 556 4923
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2498 2827 3962 4643 4834 826
Size: 219 1453 329 1135 681 191 1471
Fragments arranged by size:
1471 1453 1135 681 329 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2015 2015
Size: 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2022 2806 1872
Size: 150 784 4545
Fragments arranged by size:
4545 784 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2041 3583 4484 4746 820
Size: 197 926 98 1542 901 262 1553
Fragments arranged by size:
1553 1542 926 901 262 197 98
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2100 2837 1132
Size: 968 737 3774
Fragments arranged by size:
3774 968 737
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2004 2407 2918 513
Size: 552 939 403 511 3074
Fragments arranged by size:
3074 939 552 511 403
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2048 3506 3523 3676 3861 4306 325
Size: 1255 468 1458 17 153 185 445 1498
Fragments arranged by size:
1498 1458 1255 468 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3725 5437 96
Size: 274 80 33 51 1443 1748 1712 138
Fragments arranged by size:
1748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 5447
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 2041 1943
Size: 98 5381
XmaI C'CCGG_G
Cuts at: 1870 2020 2804 1870
Size: 150 784 4545
Fragments arranged by size:
4545 784 150
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfoI SrfI SwaI XcmI