Restriction map for
pFN21AB6305
(Circular) (Six-base) MAPSORT of: pFN21AB6305.seq Check: 2860 from: 1 to: 5479
pFN21AB6305 5479 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 2778 3606 3979 1563
Size: 1215 828 373 3063
Fragments arranged by size:
3063 1215 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3542 4926 5194 1632
Size: 1910 1384 268 1917
Fragments arranged by size:
1917 1910 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 4378 5355 1203
Size: 3175 977 1327
Fragments arranged by size:
3175 1327 977
AgeI A'CCGG_T
Cuts at: 2118 4310 2118
Size: 2192 3287
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 2145 2573 1166
Size: 979 428 4072
Fragments arranged by size:
4072 979 428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2580 2612 2580
Size: 32 5447
AlwNI CAG_nnn'CTG
Cuts at: 2436 2491 3222 4794 2436
Size: 55 731 1572 3121
Fragments arranged by size:
3121 1572 731 55
ApaI G_GGCC'C
Cuts at: 2630 2630
Size: 5479
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2086 2788 3014 3166 5275 1493
Size: 593 702 226 152 2109 1697
Fragments arranged by size:
2109 1697 702 593 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2804 1870
Size: 73 861 4545
Fragments arranged by size:
4545 861 73
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2800 4255 618
Size: 325 217 627 1013 1455 1842
Fragments arranged by size:
1842 1455 1013 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2555 2630 2798 1391
Size: 1164 75 168 4072
Fragments arranged by size:
4072 1164 168 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2716 928
Size: 971 317 500 3691
Fragments arranged by size:
3691 971 500 317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3670 3704 1903
Size: 34 50 34 1649 34 3678
Fragments arranged by size:
3678 1649 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2420 4090 136
Size: 107 122 71 631 605 748 1670 1525
Fragments arranged by size:
1670 1525 748 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 2630 3540 4696 1790
Size: 840 910 1156 2573
Fragments arranged by size:
2573 1156 910 840
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 2557 3210
Size: 416 53 83 399 24 765 375 653
Cuts at: 3210 4168 442
Size: 958 1753
Fragments arranged by size:
1753 958 765 653 416 399 375 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2145 2250 2518 2692 4139 1239
Size: 857 49 105 268 174 1447 2579
Fragments arranged by size:
2579 1447 857 268 174 105 49
Bpu10I CC'TnA_GC
Cuts at: 2243 2243
Size: 5479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2226 2740 4142 882
Size: 617 727 514 1402 2219
Fragments arranged by size:
2219 1402 727 617 514
BsaAI yAC'GTr
Cuts at: 493 1204 2272 3270 493
Size: 711 1068 998 2702
Fragments arranged by size:
2702 1068 998 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3286 3668 4303 275
Size: 53 83 186 936 1753 382 635 1451
Fragments arranged by size:
1753 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2041 2118 3912 4310 4584 4731 5142 2041
Size: 77 1794 398 274 147 411 2378
Fragments arranged by size:
2378 1794 411 398 274 147 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 2580 2610 1178
Size: 30 165 30 1134 30 13 30 4047
Fragments arranged by size:
4047 1134 165 30 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2530 1186
Size: 1344 4135
BseYI C'CCAG_C
Cuts at: 2389 2451 4682 5178 2389
Size: 62 2231 496 2690
Fragments arranged by size:
2690 2231 496 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 4342
Fragments arranged by size:
4342 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3540 3625 4696 3540
Size: 85 1071 4323
Fragments arranged by size:
4323 1071 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2555 2630 2798 3540 3625 4696 1391
Size: 399 765 75 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 765 742 399 168 85 75
BspEI T'CCGG_A
Cuts at: 2041 5142 2041
Size: 3101 2378
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2514 2834 844
Size: 1670 320 3489
Fragments arranged by size:
3489 1670 320
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 1394
Size: 429 1543 3507
Fragments arranged by size:
3507 1543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2635 3974 4148 65
Size: 1183 1387 1339 174 1396
Fragments arranged by size:
1396 1387 1339 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2118 2327 4123 4310 1700
Size: 110 308 209 1796 187 2869
Fragments arranged by size:
2869 1796 308 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstXI CCAn_nnnn'nTGG
Cuts at: 2255 2255
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2571 2665 513
Size: 552 1506 94 3327
Fragments arranged by size:
3327 1506 552 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2323 2752 487
Size: 683 56 1097 429 3214
Fragments arranged by size:
3214 1097 683 429 56
BtsI GCAGTG_nn'
Cuts at: 2191 2995 3789 3809 2191
Size: 804 794 20 3861
Fragments arranged by size:
3861 804 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DraIII CAC_nnn'GTG
Cuts at: 2197 2197
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444
Size: 54 1425 50 275 18 1096 889 1629
Cuts at: 5444 8
Size: 43
Fragments arranged by size:
1629 1425 1096 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3409 1353
Size: 2056 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2145 2250 2518 2692 3542 4139
Size: 393 464 49 105 268 174 850 597
Cuts at: 4139 4926 5194 1239
Size: 787 268 1524
Fragments arranged by size:
1524 850 787 597 464 393 268 268
174 105 49
EcoNI CCTnn'n_nnAGG
Cuts at: 2495 2495
Size: 5479
EcoO109I rG'GnC_Cy
Cuts at: 2035 2154 2626 2627 2035
Size: 119 472 1 4887
Fragments arranged by size:
4887 472 119 1
EcoRI G'AATT_C
Cuts at: 2086 2788 3166 2086
Size: 702 378 4399
Fragments arranged by size:
4399 702 378
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 4626 1678
Size: 2948 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2173 2205 4120 4152 4194
Size: 32 135 32 601 32 1915 32 42
Cuts at: 4194 4226 1373
Size: 32 2626
Fragments arranged by size:
2626 1915 601 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 2799 4593 4777 1305
Size: 739 755 1794 184 2007
Fragments arranged by size:
2007 1794 755 739 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2573 3437 3796 3955 518
Size: 648 979 428 864 359 159 2042
Fragments arranged by size:
2042 979 864 648 428 359 159
MspA1I CmG'CkG
Cuts at: 1606 2186 2389 2479 2895 3572 4720 4965 1606
Size: 580 203 90 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 580 416 245 203 90
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 2571 2665 513
Size: 552 1506 94 3327
Fragments arranged by size:
3327 1506 552 94
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2641 1108
Size: 1533 3946
PfoI T'CCnGG_A
Cuts at: 2708 2750 2708
Size: 42 5437
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2580 2612 3548 3580 5061 5093 2580
Size: 32 936 32 1481 32 2966
Fragments arranged by size:
2966 1481 936 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2035 2154 2035
Size: 119 5360
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2626 2626
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2496 2831 830
Size: 1666 335 3478
Fragments arranged by size:
3478 1666 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2186 2389 2479 2186
Size: 203 90 5186
Fragments arranged by size:
5186 203 90
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SanDI GG'GwC_CC
Cuts at: 2154 2154
Size: 5479
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2492 2718 2827 3962 4643 4834 826
Size: 219 1447 226 109 1135 681 191 1471
Fragments arranged by size:
1471 1447 1135 681 226 219 191 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2571 2665 2918 513
Size: 552 1506 94 253 3074
Fragments arranged by size:
3074 1506 552 253 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2164 2381 2744 3506 3523 3676
Size: 1255 465 119 217 363 762 17 153
Cuts at: 3676 3861 4306 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 762 465 445 363 217 185
153 119 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3725 5437 96
Size: 274 80 33 51 1443 1748 1712 138
Fragments arranged by size:
1748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 5447
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2476 2618 2120
Size: 356 142 4981
Fragments arranged by size:
4981 356 142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 2213 3608 2213
Size: 1395 4084
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI