Restriction map for
pFN21AB6301
(Circular) (Six-base) MAPSORT of: pFN21AB6301.seq Check: 6347 from: 1 to: 5455
pFN21AB6301 5455 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 2233
Size: 5455
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 278
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2238 2798 1937
Size: 301 560 4594
Fragments arranged by size:
4594 560 301
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3518 4902 5170 1632
Size: 1886 1384 268 1917
Fragments arranged by size:
1917 1886 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 4354 5331 1203
Size: 3151 977 1327
Fragments arranged by size:
3151 1327 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5455
AlwNI CAG_nnn'CTG
Cuts at: 3198 4770 3198
Size: 1572 3883
ApaLI G'TGCA_C
Cuts at: 3512 4668 3512
Size: 1156 4299
ApoI r'AATT_y
Cuts at: 1493 2709 2764 2990 3142 5251 1493
Size: 1216 55 226 152 2109 1697
Fragments arranged by size:
2109 1697 1216 226 152 55
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2141 2780 1870
Size: 73 198 639 4545
Fragments arranged by size:
4545 639 198 73
BamHI G'GATC_C
Cuts at: 2785 3152 2785
Size: 367 5088
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2149 2176 2186 2327 2383
Size: 325 217 627 362 27 10 141 56
Cuts at: 2383 2776 4231 618
Size: 393 1455 1842
Fragments arranged by size:
1842 1455 627 393 362 325 217 141
56 27 10
BanII G_rGCy'C
Cuts at: 1391 2774 1391
Size: 1383 4072
BbeI G_GCGC'C
Cuts at: 2190 2331 2190
Size: 141 5314
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3646 3680 1903
Size: 34 1709 34 3678
Fragments arranged by size:
3678 1709 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2325 4066 136
Size: 107 122 71 631 605 653 1741 1525
Fragments arranged by size:
1741 1525 653 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2872 2872
Size: 5455
Bme1580I G_kGCm'C
Cuts at: 1790 2414 3516 4672 1790
Size: 624 1102 1156 2573
Fragments arranged by size:
2573 1156 1102 624
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2554 2714 3186
Size: 416 53 83 399 24 1137 160 472
Cuts at: 3186 4144 442
Size: 958 1753
Fragments arranged by size:
1753 1137 958 472 416 399 160 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 2217 2226 2253 4115 1239
Size: 927 51 9 27 1862 2579
Fragments arranged by size:
2579 1862 927 51 27 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2046 3580 4445 4743 2046
Size: 1534 865 298 2758
Fragments arranged by size:
2758 1534 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4118 882
Size: 617 2619 2219
Fragments arranged by size:
2619 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 5449 5449
Size: 5455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 2328 3262 3644
Size: 53 83 186 936 654 141 934 382
Cuts at: 3644 4279 275
Size: 635 1451
Fragments arranged by size:
1451 936 934 654 635 382 186 141
83 53
BsaWI w'CCGG_w
Cuts at: 2297 3888 4286 4560 4707 5118 2297
Size: 1591 398 274 147 411 2634
Fragments arranged by size:
2634 1591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5230
Fragments arranged by size:
5230 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5455
BseYI C'CCAG_C
Cuts at: 2096 2252 2336 4658 5154 2096
Size: 156 84 2322 496 2397
Fragments arranged by size:
2397 2322 496 156 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2211 1163
Size: 573 475 4407
Fragments arranged by size:
4407 573 475
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2203 2224 3516 3601 4672 2203
Size: 21 1292 85 1071 2986
Fragments arranged by size:
2986 1292 1071 85 21
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2203 2224 2414 2774 3516 3601 4672
Size: 399 413 21 190 360 742 85 1071
Cuts at: 4672 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 413 399 360 190 85
21
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 2370 2810 844
Size: 1389 137 440 3489
Fragments arranged by size:
3489 1389 440 137
BsrBI CCG'CTC
Cuts at: 1394 1823 3342 1394
Size: 429 1519 3507
Fragments arranged by size:
3507 1519 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3950 4124 65
Size: 1183 2702 174 1396
Fragments arranged by size:
2702 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4099 4286 1700
Size: 110 2289 187 2869
Fragments arranged by size:
2869 2289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5455
BssSI C'ACGA_G
Cuts at: 3515 4527 3515
Size: 1012 4443
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5455
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5455
BstXI CCAn_nnnn'nTGG
Cuts at: 2096 2096
Size: 5455
BstYI r'GATC_y
Cuts at: 1972 2785 3152 3537 3554 5006 5092 5104 5450
Size: 813 367 385 17 1452 86 12 346
Cuts at: 5450 1972
Size: 1977
Fragments arranged by size:
1977 1452 813 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3964
Fragments arranged by size:
3964 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4716
Fragments arranged by size:
4716 683 56
BtsI GCAGTG_nn'
Cuts at: 2646 2971 3765 3785 2646
Size: 325 794 20 4316
Fragments arranged by size:
4316 794 325 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420
Size: 54 1425 50 275 18 1072 889 1629
Cuts at: 5420 8
Size: 43
Fragments arranged by size:
1629 1425 1072 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3385 1353
Size: 2032 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4042 4416 4562 387
Size: 1064 138 2453 374 146 1280
Fragments arranged by size:
2453 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2217 2226 2253 3518 4115 4902
Size: 393 534 51 9 27 1265 597 787
Cuts at: 4902 5170 1239
Size: 268 1524
Fragments arranged by size:
1524 1265 787 597 534 393 268 51
27 9
EcoO109I rG'GnC_Cy
Cuts at: 2133 2265 2133
Size: 132 5323
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 2190 2331 4602 1678
Size: 512 141 2271 2531
Fragments arranged by size:
2531 2271 512 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 4096 4128 4170
Size: 32 135 32 449 32 2043 32 42
Cuts at: 4170 4202 1373
Size: 32 2626
Fragments arranged by size:
2626 2043 449 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2799 3045 669
Size: 988 281 861 246 3079
Fragments arranged by size:
3079 988 861 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KasI G'GCGC_C
Cuts at: 2186 2327 2186
Size: 141 5314
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4569 4753 1305
Size: 3264 184 2007
Fragments arranged by size:
3264 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3633
Fragments arranged by size:
3633 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3413 3772 3931 518
Size: 648 2247 359 159 2042
Fragments arranged by size:
2247 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2256 2871 3548 4696 4941 1606
Size: 650 615 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 650 615 245
NarI GG'CG_CC
Cuts at: 2187 2328 2187
Size: 141 5314
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3964
Fragments arranged by size:
3964 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5455
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2395 2813 4358 1132
Size: 97 1166 418 1545 2229
Fragments arranged by size:
2229 1545 1166 418 97
PasI CC'CwG_GG
Cuts at: 1348 2114 2156 2243 2261 1348
Size: 766 42 87 18 4542
Fragments arranged by size:
4542 766 87 42 18
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2279 1108
Size: 1171 4284
PfoI T'CCnGG_A
Cuts at: 2167 2215 2167
Size: 48 5407
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 5037 5069 3524
Size: 32 1481 32 3910
Fragments arranged by size:
3910 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5455
PsiI TTA'TAA
Cuts at: 2358 3025 2358
Size: 667 4788
PstI C_TGCA'G
Cuts at: 830 2807 830
Size: 1977 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 2610 3703 5415 2610
Size: 1093 1712 2650
Fragments arranged by size:
2650 1712 1093
SfcI C'TryA_G
Cuts at: 826 1045 2803 3938 4619 4810 826
Size: 219 1758 1135 681 191 1471
Fragments arranged by size:
1758 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2188 2329 2188
Size: 141 5314
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5455
SmaI CCC'GGG
Cuts at: 1872 2143 2782 1872
Size: 271 639 4545
Fragments arranged by size:
4545 639 271
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2061 3559 4460 4722 820
Size: 197 926 118 1498 901 262 1553
Fragments arranged by size:
1553 1498 926 901 262 197 118
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2395 2813 1132
Size: 1263 418 3774
Fragments arranged by size:
3774 1263 418
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5455
StyI C'CwwG_G
Cuts at: 513 1065 2004 2894 513
Size: 552 939 890 3074
Fragments arranged by size:
3074 939 890 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 3482 3499 3652 3837 4282 325
Size: 1255 686 1216 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1216 686 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2608 2664 3701
Size: 274 80 33 51 1443 631 56 1037
Cuts at: 3701 5413 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1037 631 274 138 80 56
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5415 5447 5415
Size: 32 5423
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2141 2780 1870
Size: 271 639 4545
Fragments arranged by size:
4545 639 271
XmnI GAAnn'nnTTC
Cuts at: 2594 3584 2594
Size: 990 4465
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 276
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI