Restriction map for
pFN21AB6296
(Circular) (Six-base) MAPSORT of: pFN21AB6296.seq Check: 3728 from: 1 to: 7081
pFN21AB6296 7081 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4891 278
Size: 53 83 186 936 3355 2468
Fragments arranged by size:
3355 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2157 3146 4424 1937
Size: 220 989 1278 4594
Fragments arranged by size:
4594 1278 989 220
Acc65I G'GTAC_C
Cuts at: 2246 4402 2246
Size: 2156 4925
AclI AA'CG_TT
Cuts at: 1563 5208 5581 1563
Size: 3645 373 3063
Fragments arranged by size:
3645 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3198 3462 3687 5144 6528 6796 1632
Size: 1566 264 225 1457 1384 268 1917
Fragments arranged by size:
1917 1566 1457 1384 268 264 225
AflII C'TTAA_G
Cuts at: 820 1017 3917 4214 820
Size: 197 2900 297 3687
Fragments arranged by size:
3687 2900 297 197
AflIII A'CryG_T
Cuts at: 1203 5980 6957 1203
Size: 4777 977 1327
Fragments arranged by size:
4777 1327 977
AgeI A'CCGG_T
Cuts at: 5912 5912
Size: 7081
AhdI GACnn_n'nnGTC
Cuts at: 5810 5810
Size: 7081
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7081
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3511 3543 3511
Size: 32 7049
AlwNI CAG_nnn'CTG
Cuts at: 4824 6396 4824
Size: 1572 5509
ApaLI G'TGCA_C
Cuts at: 5138 6294 5138
Size: 1156 5925
ApoI r'AATT_y
Cuts at: 1493 2929 3810 4010 4059 4390 4616 4768 6877
Size: 1436 881 200 49 331 226 152 2109
Cuts at: 6877 1493
Size: 1697
Fragments arranged by size:
2109 1697 1436 881 331 226 200 152
49
AseI AT'TA_AT
Cuts at: 160 1032 5635 160
Size: 872 4603 1606
Fragments arranged by size:
4603 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7081
AvaI C'yCGr_G
Cuts at: 1870 1943 4406 1870
Size: 73 2463 4545
Fragments arranged by size:
4545 2463 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2886 2919 3525 3558 2886
Size: 33 606 33 6409
Fragments arranged by size:
6409 606 33 33
BamHI G'GATC_C
Cuts at: 4411 4778 4411
Size: 367 6714
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 3079 4402 5857 618
Size: 325 217 627 459 833 1323 1455 1842
Fragments arranged by size:
1842 1455 1323 833 627 459 325 217
BanII G_rGCy'C
Cuts at: 1391 4045 4400 1391
Size: 2654 355 4072
Fragments arranged by size:
4072 2654 355
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5272 5306 1903
Size: 34 3335 34 3678
Fragments arranged by size:
3678 3335 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2872 3553 3941 4091 4973 6183 2872
Size: 681 388 150 882 1210 3770
Fragments arranged by size:
3770 1210 882 681 388 150
BclI T'GATC_A
Cuts at: 1548 2109 3099 1548
Size: 561 990 5530
Fragments arranged by size:
5530 990 561
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4145 5692
Size: 107 122 71 631 605 337 2136 1547
Cuts at: 5692 136
Size: 1525
Fragments arranged by size:
2136 1547 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3928 7076 3928
Size: 3148 3933
BlpI GC'TnA_GC
Cuts at: 4498 4498
Size: 7081
Bme1580I G_kGCm'C
Cuts at: 1790 5142 6298 1790
Size: 3352 1156 2573
Fragments arranged by size:
3352 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2155 1318
Size: 837 6244
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4812 5770 442
Size: 416 53 83 399 24 3395 958 1753
Fragments arranged by size:
3395 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7081
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2050 2196 4059 5741 1239
Size: 811 146 1863 1682 2579
Fragments arranged by size:
2579 1863 1682 811 146
Bpu10I CC'TnA_GC
Cuts at: 3452 3452
Size: 7081
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3072 5206 6071 6369 3072
Size: 2134 865 298 3784
Fragments arranged by size:
3784 2134 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5744 882
Size: 617 4245 2219
Fragments arranged by size:
4245 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4872 493
Size: 711 3668 2702
Fragments arranged by size:
3668 2702 711
BsaBI GATnn'nnATC
Cuts at: 2111 7075 2111
Size: 4964 2117
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4888 5270 5905 275
Size: 53 83 186 936 3355 382 635 1451
Fragments arranged by size:
3355 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5514 5912 6186 6333 6744 5514
Size: 398 274 147 411 5851
Fragments arranged by size:
5851 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6856
Fragments arranged by size:
6856 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2214 1186
Size: 826 202 6053
Fragments arranged by size:
6053 826 202
BseYI C'CCAG_C
Cuts at: 2015 3579 4238 6284 6780 2015
Size: 1564 659 2046 496 2316
Fragments arranged by size:
2316 2046 1564 659 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 1163
Size: 573 399 6109
Fragments arranged by size:
6109 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4531 5292 6320 1540
Size: 275 186 2530 761 1028 2301
Fragments arranged by size:
2530 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2656 4045 5142 5227 6298 2656
Size: 1389 1097 85 1071 3439
Fragments arranged by size:
3439 1389 1097 1071 85
BsmI GAATG_Cn'
Cuts at: 2538 4592 4685 2538
Size: 2054 93 4934
Fragments arranged by size:
4934 2054 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2656 4045 4400 5142 5227 6298 1391
Size: 399 866 1389 355 742 85 1071 2174
Fragments arranged by size:
2174 1389 1071 866 742 399 355 85
BspEI T'CCGG_A
Cuts at: 6744 6744
Size: 7081
BspHI T'CATG_A
Cuts at: 2830 4194 4970 6700 2830
Size: 1364 776 1730 3211
Fragments arranged by size:
3211 1730 1364 776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4436 844
Size: 3592 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2087 4968 1394
Size: 429 264 2881 3507
Fragments arranged by size:
3507 2881 429 264
BsrDI GCAATG_nn'
Cuts at: 65 1248 2302 5576 5750 65
Size: 1183 1054 3274 174 1396
Fragments arranged by size:
3274 1396 1183 1054 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 3076 5725 5912 1700
Size: 110 246 1020 2649 187 2869
Fragments arranged by size:
2869 2649 1020 246 187 110
BsrGI T'GTAC_A
Cuts at: 96 2775 96
Size: 2679 4402
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7081
BssSI C'ACGA_G
Cuts at: 5141 6153 5141
Size: 1012 6069
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3416 3692 794
Size: 2622 276 4183
Fragments arranged by size:
4183 2622 276
BstBI TT'CG_AA
Cuts at: 5885 5885
Size: 7081
BstEII G'GTnAC_C
Cuts at: 1184 3574 1184
Size: 2390 4691
BstYI r'GATC_y
Cuts at: 1972 3046 3928 4411 4778 5163 5180 6632 6718
Size: 1074 882 483 367 385 17 1452 86
Cuts at: 6718 6730 7076 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1074 882 483 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2227 513
Size: 552 939 223 5367
Fragments arranged by size:
5367 939 552 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 487
Size: 683 56 896 5446
Fragments arranged by size:
5446 896 683 56
BtsI GCAGTG_nn'
Cuts at: 3686 4597 5391 5411 3686
Size: 911 794 20 5356
Fragments arranged by size:
5356 911 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7081
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3747 3782 3961 3996 4325 4360 529
Size: 35 3183 35 179 35 329 35 3250
Fragments arranged by size:
3250 3183 329 179 35 35 35 35
DraI TTT'AAA
Cuts at: 2484 3367 3817 4015 4377 4385 2484
Size: 883 450 198 362 8 5180
Fragments arranged by size:
5180 883 450 362 198 8
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6088 809
Size: 507 4772 1802
Fragments arranged by size:
4772 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2953 3768
Size: 54 1425 50 275 18 261 862 815
Cuts at: 3768 4528 5417 7046 8
Size: 760 889 1629 43
Fragments arranged by size:
1629 1425 889 862 815 760 275 261
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4528 1537
Size: 275 2716 4090
Fragments arranged by size:
4090 2716 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2995 5011 1353
Size: 1642 2016 3423
Fragments arranged by size:
3423 2016 1642
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5668 6042 6188 387
Size: 1064 138 4079 374 146 1280
Fragments arranged by size:
4079 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4043 4043
Size: 7081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2196 3198 3462 3687 4059 5144
Size: 393 418 146 1002 264 225 372 1085
Cuts at: 5144 5741 6528 6796 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1085 1002 787 597 418 393 372
268 264 225 146
EcoO109I rG'GnC_Cy
Cuts at: 2794 3467 3989 2794
Size: 673 522 5886
Fragments arranged by size:
5886 673 522
EcoRI G'AATT_C
Cuts at: 4390 4768 4390
Size: 378 6703
EcoRV GAT'ATC
Cuts at: 2206 4299 2206
Size: 2093 4988
FspI TGC'GCA
Cuts at: 5587 5587
Size: 7081
HaeII r_GCGC'y
Cuts at: 1678 6228 1678
Size: 4550 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2190 2222 2812 2844 3128
Size: 32 135 32 618 32 590 32 284
Cuts at: 3128 3160 3235 3267 4256 4288 5722 5754 5796
Size: 32 75 32 989 32 1434 32 42
Cuts at: 5796 5828 1373
Size: 32 2626
Fragments arranged by size:
2626 1434 989 618 590 284 135 75
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4184 4425 4671 669
Size: 988 281 2246 241 246 3079
Fragments arranged by size:
3079 2246 988 281 246 241
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7081
HpaI GTT'AAC
Cuts at: 4671 4671
Size: 7081
KpnI G_GTAC'C
Cuts at: 2250 4406 2250
Size: 2156 4925
MfeI C'AATT_G
Cuts at: 4680 4680
Size: 7081
MluI A'CGCG_T
Cuts at: 6957 6957
Size: 7081
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 2513 4127 6195 6379 1305
Size: 1176 32 1614 2068 184 2007
Fragments arranged by size:
2068 2007 1614 1176 184 32
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 2955 3770 10
Size: 54 1425 343 261 862 815 3321
Fragments arranged by size:
3321 1425 862 815 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 2835 5039 5398 5557 518
Size: 648 1377 292 2204 359 159 2042
Fragments arranged by size:
2204 2042 1377 648 359 292 159
MspA1I CmG'CkG
Cuts at: 1606 2192 2588 3097 4497 5174 6322 6567 1606
Size: 586 396 509 1400 677 1148 245 2120
Fragments arranged by size:
2120 1400 1148 677 586 509 396 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2227 513
Size: 552 939 223 5367
Fragments arranged by size:
5367 939 552 223
NdeI CA'TA_TG
Cuts at: 387 3682 387
Size: 3295 3786
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7081
NotI GC'GGCC_GC
Cuts at: 4528 4528
Size: 7081
NspI r_CATG'y
Cuts at: 1132 1229 3725 4439 5984 1132
Size: 97 2496 714 1545 2229
Fragments arranged by size:
2496 2229 1545 714 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7081
PciI A'CATG_T
Cuts at: 5980 5980
Size: 7081
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2951 1108
Size: 1843 5238
PfoI T'CCnGG_A
Cuts at: 2097 4036 2097
Size: 1939 5142
PmeI GTTT'AAAC
Cuts at: 4385 4385
Size: 7081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5150 5182 6663 6695 5150
Size: 32 1481 32 5536
Fragments arranged by size:
5536 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 3467 3989 2794
Size: 673 522 5886
Fragments arranged by size:
5886 673 522
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7081
PsiI TTA'TAA
Cuts at: 4651 4651
Size: 7081
PstI C_TGCA'G
Cuts at: 830 4054 4433 830
Size: 3224 379 3478
Fragments arranged by size:
3478 3224 379
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7081
PvuII CAG'CTG
Cuts at: 2192 2588 3097 2192
Size: 396 509 6176
Fragments arranged by size:
6176 509 396
SacI G_AGCT'C
Cuts at: 4045 4045
Size: 7081
SalI G'TCGA_C
Cuts at: 1936 4423 1936
Size: 2487 4594
SanDI GG'GwC_CC
Cuts at: 2794 2794
Size: 7081
SbfI CC_TGCA'GG
Cuts at: 4433 4433
Size: 7081
ScaI AGT'ACT
Cuts at: 3550 5329 7041 3550
Size: 1779 1712 3590
Fragments arranged by size:
3590 1779 1712
SfcI C'TryA_G
Cuts at: 826 1045 2507 3147 3726 3907 4050 4429 5564
Size: 219 1462 640 579 181 143 379 1135
Cuts at: 5564 6245 6436 826
Size: 681 191 1471
Fragments arranged by size:
1471 1462 1135 681 640 579 379 219
191 181 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7081
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7081
SmaI CCC'GGG
Cuts at: 1872 4408 1872
Size: 2536 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3087 3917 4214 5185 6086 6348
Size: 197 926 1144 830 297 971 901 262
Cuts at: 6348 820
Size: 1553
Fragments arranged by size:
1553 1144 971 926 901 830 297 262
197
SnaBI TAC'GTA
Cuts at: 493 4872 493
Size: 4379 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7081
SphI G_CATG'C
Cuts at: 1132 4439 1132
Size: 3307 3774
SspI AAT'ATT
Cuts at: 5 52 2256 4123 4133 5005 5
Size: 47 2204 1867 10 872 2081
Fragments arranged by size:
2204 2081 1867 872 47 10
StuI AGG'CCT
Cuts at: 1517 4279 1517
Size: 2762 4319
StyI C'CwwG_G
Cuts at: 513 1065 2004 2227 4520 513
Size: 552 939 223 2293 3074
Fragments arranged by size:
3074 2293 939 552 223
SwaI ATTT'AAAT
Cuts at: 2484 2484
Size: 7081
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5108 5125 5278 5463 5908 325
Size: 1255 3528 17 153 185 445 1498
Fragments arranged by size:
3528 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2775 3091 3176
Size: 274 80 33 51 1443 798 316 85
Cuts at: 3176 3548 3608 5327 7039 96
Size: 372 60 1719 1712 138
Fragments arranged by size:
1719 1712 1443 798 372 316 274 138
85 80 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4192 4224 7041 7073 4192
Size: 32 2817 32 4200
Fragments arranged by size:
4200 2817 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7081
XbaI T'CTAG_A
Cuts at: 4417 4417
Size: 7081
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7081
XmaI C'CCGG_G
Cuts at: 1870 4406 1870
Size: 2536 4545
XmnI GAAnn'nnTTC
Cuts at: 5210 5210
Size: 7081
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4889 276
Size: 53 83 186 936 3355 2468
Fragments arranged by size:
3355 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SapI SexAI SfiI SfoI SrfI XcmI