Restriction map for
pFN21AB6289
(Circular) (Six-base) MAPSORT of: pFN21AB6289.seq Check: 693 from: 1 to: 6994
pFN21AB6289 6994 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2903 4101 4804 278
Size: 53 83 186 936 1367 1198 703 2468
Fragments arranged by size:
2468 1367 1198 936 703 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 1937
Size: 2400 4594
Acc65I G'GTAC_C
Cuts at: 3139 4315 3139
Size: 1176 5818
AclI AA'CG_TT
Cuts at: 1563 5121 5494 1563
Size: 3558 373 3063
Fragments arranged by size:
3558 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5057 6441 6709 1632
Size: 3425 1384 268 1917
Fragments arranged by size:
3425 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6797
AflIII A'CryG_T
Cuts at: 1203 2883 5893 6870 1203
Size: 1680 3010 977 1327
Fragments arranged by size:
3010 1680 1327 977
AgeI A'CCGG_T
Cuts at: 5825 5825
Size: 6994
AhdI GACnn_n'nnGTC
Cuts at: 2992 5723 2992
Size: 2731 4263
AleI CACnn'nnGTG
Cuts at: 1166 2940 1166
Size: 1774 5220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2542 4223 1184
Size: 1358 1681 3955
Fragments arranged by size:
3955 1681 1358
AlwNI CAG_nnn'CTG
Cuts at: 3169 4737 6309 3169
Size: 1568 1572 3854
Fragments arranged by size:
3854 1572 1568
ApaI G_GGCC'C
Cuts at: 2313 3702 4253 2313
Size: 1389 551 5054
Fragments arranged by size:
5054 1389 551
ApaLI G'TGCA_C
Cuts at: 5051 6207 5051
Size: 1156 5838
ApoI r'AATT_y
Cuts at: 1493 2318 4303 4529 4681 6790 1493
Size: 825 1985 226 152 2109 1697
Fragments arranged by size:
2109 1985 1697 825 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5548 160
Size: 872 4516 1606
Fragments arranged by size:
4516 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 3838 4319 1870
Size: 73 1065 830 481 4545
Fragments arranged by size:
4545 1065 830 481 73
BamHI G'GATC_C
Cuts at: 4324 4691 4324
Size: 367 6627
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3139 3193 3938 4315 5770
Size: 325 217 627 1352 54 745 377 1455
Cuts at: 5770 618
Size: 1842
Fragments arranged by size:
1842 1455 1352 745 627 377 325 217
54
BanII G_rGCy'C
Cuts at: 1391 2313 2347 2479 2594 3051 3227 3449 3702
Size: 922 34 132 115 457 176 222 253
Cuts at: 3702 3729 4253 4313 1391
Size: 27 524 60 4072
Fragments arranged by size:
4072 922 524 457 253 222 176 132
115 60 34 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6023
BbvCI CC'TCA_GC
Cuts at: 3282 4196 3282
Size: 914 6080
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5185 5219 1903
Size: 34 3248 34 3678
Fragments arranged by size:
3678 3248 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4886 6096 4886
Size: 1210 5784
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6994
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5605 136
Size: 107 122 71 631 605 3933 1525
Fragments arranged by size:
3933 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6989 6989
Size: 6994
BlpI GC'TnA_GC
Cuts at: 3767 4411 3767
Size: 644 6350
Bme1580I G_kGCm'C
Cuts at: 1790 2313 3702 4253 5055 6211 1790
Size: 523 1389 551 802 1156 2573
Fragments arranged by size:
2573 1389 1156 802 551 523
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6994
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2597 2934 3696
Size: 416 53 83 399 24 1180 337 762
Cuts at: 3696 3914 4256 4267 4725 5683 442
Size: 218 342 11 458 958 1753
Fragments arranged by size:
1753 1180 958 762 458 416 399 342
337 218 83 53 24 11
BmtI G_CTAG'C
Cuts at: 1056 3281 1056
Size: 2225 4769
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 3484 3651 2182
Size: 1302 167 5525
Fragments arranged by size:
5525 1302 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2343 2438 2493 2660 3895 5654 1239
Size: 1104 95 55 167 1235 1759 2579
Fragments arranged by size:
2579 1759 1235 1104 167 95 55
Bpu10I CC'TnA_GC
Cuts at: 3282 3563 4196 4284 3282
Size: 281 633 88 5992
Fragments arranged by size:
5992 633 281 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5119 5984 6282 5119
Size: 865 298 5831
Fragments arranged by size:
5831 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2414 2492 2668 2923 3000 3421 3639
Size: 617 915 78 176 255 77 421 218
Cuts at: 3639 5657 882
Size: 2018 2219
Fragments arranged by size:
2219 2018 915 617 421 255 218 176
78 77
BsaAI yAC'GTr
Cuts at: 493 1204 2796 4785 493
Size: 711 1592 1989 2702
Fragments arranged by size:
2702 1989 1592 711
BsaBI GATnn'nnATC
Cuts at: 2454 6988 2454
Size: 4534 2460
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2900 4098 4801 5183
Size: 53 83 186 936 1367 1198 703 382
Cuts at: 5183 5818 275
Size: 635 1451
Fragments arranged by size:
1451 1367 1198 936 703 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5427 5825 6099 6246 6657 5427
Size: 398 274 147 411 5764
Fragments arranged by size:
5764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2341 2371 3503 3533 3743
Size: 30 165 30 938 30 1132 30 210
Cuts at: 3743 3773 4043 4073 1178
Size: 30 270 30 4099
Fragments arranged by size:
4099 1132 938 270 210 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2678 3288 3299 3341 3631 3640 3736
Size: 919 573 610 11 42 290 9 96
Cuts at: 3736 3820 4022 1186
Size: 84 202 4158
Fragments arranged by size:
4158 919 610 573 290 202 96 84
42 11 9
BseYI C'CCAG_C
Cuts at: 2183 3714 3736 3762 3889 6197 6693 2183
Size: 1531 22 26 127 2308 496 2484
Fragments arranged by size:
2484 2308 1531 496 127 26 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2512 2526 2861 1163
Size: 573 776 14 335 5296
Fragments arranged by size:
5296 776 573 335 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3038 4444 5205 6233 1540
Size: 275 186 1037 1406 761 1028 2301
Fragments arranged by size:
2301 1406 1037 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3015 3051 3227 3729 4006 5055 5140 6211 3015
Size: 36 176 502 277 1049 85 1071 3798
Fragments arranged by size:
3798 1071 1049 502 277 176 85 36
BsmI GAATG_Cn'
Cuts at: 4505 4598 4505
Size: 93 6901
BsmBI CGTCTCn'nnnn_
Cuts at: 2891 2891
Size: 6994
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2347 2479 2594 3015 3051 3227
Size: 399 523 34 132 115 421 36 176
Cuts at: 3227 3449 3702 3729 4006 4253 4313 5055 5140
Size: 222 253 27 277 247 60 742 85
Cuts at: 5140 6211 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 523 421 399 277 253
247 222 176 132 115 85 60 36
34 27
BspEI T'CCGG_A
Cuts at: 6657 6657
Size: 6994
BspHI T'CATG_A
Cuts at: 3219 4883 6613 3219
Size: 1664 1730 3600
Fragments arranged by size:
3600 1730 1664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2446 4349 844
Size: 1602 1903 3489
Fragments arranged by size:
3489 1903 1602
BsrBI CCG'CTC
Cuts at: 1394 1823 2710 4881 1394
Size: 429 887 2171 3507
Fragments arranged by size:
3507 2171 887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2829 3197 3258 5489 5663 65
Size: 1183 1581 368 61 2231 174 1396
Fragments arranged by size:
2231 1581 1396 1183 368 174 61
BsrFI r'CCGG_y
Cuts at: 1700 1810 3630 5638 5825 1700
Size: 110 1820 2008 187 2869
Fragments arranged by size:
2869 2008 1820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6994
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6994
BssSI C'ACGA_G
Cuts at: 5054 6066 5054
Size: 1012 5982
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2279 3655 794
Size: 1485 1376 4133
Fragments arranged by size:
4133 1485 1376
BstBI TT'CG_AA
Cuts at: 2566 5798 2566
Size: 3232 3762
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6994
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 3439 2152
Size: 1287 5707
BstYI r'GATC_y
Cuts at: 1972 2105 3976 4203 4324 4691 5076 5093 6545
Size: 133 1871 227 121 367 385 17 1452
Cuts at: 6545 6631 6643 6989 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1871 1452 385 367 346 227 133
121 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3119 513
Size: 552 2054 4388
Fragments arranged by size:
4388 2054 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6255
Fragments arranged by size:
6255 683 56
BtsI GCAGTG_nn'
Cuts at: 3110 4510 5304 5324 3110
Size: 1400 794 20 4780
Fragments arranged by size:
4780 1400 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6959
DraI TTT'AAA
Cuts at: 4298 4298
Size: 6994
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2888 6001 809
Size: 507 1572 3113 1802
Fragments arranged by size:
3113 1802 1572 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2232 2711
Size: 54 1425 50 275 18 194 208 479
Cuts at: 2711 2730 4441 5330 6959 8
Size: 19 1711 889 1629 43
Fragments arranged by size:
1711 1629 1425 889 479 275 208 194
54 50 43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 4441 1537
Size: 275 2629 4090
Fragments arranged by size:
4090 2629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3453 3692 4084 4924 1353
Size: 2100 239 392 840 3423
Fragments arranged by size:
3423 2100 840 392 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2849 3041 3112 5581 5955 6101
Size: 1064 138 1260 192 71 2469 374 146
Cuts at: 6101 387
Size: 1280
Fragments arranged by size:
2469 1280 1260 1064 374 192 146 138
71
EcoICRI GAG'CTC
Cuts at: 3049 3225 3727 3049
Size: 176 502 6316
Fragments arranged by size:
6316 502 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 2438 2493 2660 3895 5057 5654
Size: 393 711 95 55 167 1235 1162 597
Cuts at: 5654 6441 6709 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1235 1162 787 711 597 393 268
167 95 55
EcoNI CCTnn'n_nnAGG
Cuts at: 4235 4235
Size: 6994
EcoO109I rG'GnC_Cy
Cuts at: 3567 3698 3791 4240 4250 3567
Size: 131 93 449 10 6311
Fragments arranged by size:
6311 449 131 93 10
EcoRI G'AATT_C
Cuts at: 4303 4681 4303
Size: 378 6616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 6994
FspI TGC'GCA
Cuts at: 5500 5500
Size: 6994
HaeII r_GCGC'y
Cuts at: 1678 6141 1678
Size: 4463 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2978 3010 3283 3315 3482
Size: 32 135 32 1406 32 273 32 167
Cuts at: 3482 3503 3514 3535 3743 3775 4043 4075 5635
Size: 21 11 21 208 32 268 32 1560
Cuts at: 5635 5667 5709 5741 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1560 1406 273 268 208 167 135
42 32 32 32 32 32 32 32
32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4338 4584 669
Size: 988 281 2400 246 3079
Fragments arranged by size:
3079 2400 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6994
HpaI GTT'AAC
Cuts at: 4584 4584
Size: 6994
KpnI G_GTAC'C
Cuts at: 3143 4319 3143
Size: 1176 5818
MfeI C'AATT_G
Cuts at: 3002 4593 3002
Size: 1591 5403
MluI A'CGCG_T
Cuts at: 6870 6870
Size: 6994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 2574 6108 6292 1305
Size: 882 387 3534 184 2007
Fragments arranged by size:
3534 2007 882 387 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2732 10
Size: 54 1425 343 402 498 4272
Fragments arranged by size:
4272 1425 498 402 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 2940 3437 4952 5311 5470 518
Size: 648 1566 208 497 1515 359 159 2042
Fragments arranged by size:
2042 1566 1515 648 497 359 208 159
MspA1I CmG'CkG
Cuts at: 1606 2183 2334 3714 4410 5087 6235 6480 1606
Size: 577 151 1380 696 677 1148 245 2120
Fragments arranged by size:
2120 1380 1148 696 677 577 245 151
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6442
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6994
NheI G'CTAG_C
Cuts at: 1052 3277 1052
Size: 2225 4769
NotI GC'GGCC_GC
Cuts at: 4441 4441
Size: 6994
NspI r_CATG'y
Cuts at: 1132 1229 2887 4352 5897 1132
Size: 97 1658 1465 1545 2229
Fragments arranged by size:
2229 1658 1545 1465 97
PasI CC'CwG_GG
Cuts at: 1348 2990 3085 3934 3964 1348
Size: 1642 95 849 30 4378
Fragments arranged by size:
4378 1642 849 95 30
PciI A'CATG_T
Cuts at: 2883 5893 2883
Size: 3010 3984
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3169 3784 1108
Size: 2061 615 4318
Fragments arranged by size:
4318 2061 615
PfoI T'CCnGG_A
Cuts at: 2359 2892 3872 4173 2359
Size: 533 980 301 5180
Fragments arranged by size:
5180 980 533 301
PmeI GTTT'AAAC
Cuts at: 4298 4298
Size: 6994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5063 5095 6576 6608 3716
Size: 32 1315 32 1481 32 4102
Fragments arranged by size:
4102 1481 1315 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3567 3791 4240 3567
Size: 224 449 6321
Fragments arranged by size:
6321 449 224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6994
PsiI TTA'TAA
Cuts at: 4564 4564
Size: 6994
PspOMI G'GGCC_C
Cuts at: 2309 3698 4249 2309
Size: 1389 551 5054
Fragments arranged by size:
5054 1389 551
PstI C_TGCA'G
Cuts at: 830 2443 3313 4346 830
Size: 1613 870 1033 3478
Fragments arranged by size:
3478 1613 1033 870
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6994
PvuII CAG'CTG
Cuts at: 2183 2334 2183
Size: 151 6843
SacI G_AGCT'C
Cuts at: 3051 3227 3729 3051
Size: 176 502 6316
Fragments arranged by size:
6316 502 176
SalI G'TCGA_C
Cuts at: 1936 4336 1936
Size: 2400 4594
SapI GCTCTTCn'nnn_
Cuts at: 3692 3692
Size: 6994
SbfI CC_TGCA'GG
Cuts at: 4346 4346
Size: 6994
ScaI AGT'ACT
Cuts at: 3706 5242 6954 3706
Size: 1536 1712 3746
Fragments arranged by size:
3746 1712 1536
SexAI A'CCwGG_T
Cuts at: 2967 3135 2967
Size: 168 6826
SfcI C'TryA_G
Cuts at: 826 1045 2439 3309 3553 4289 4342 5477 6158
Size: 219 1394 870 244 736 53 1135 681
Cuts at: 6158 6349 826
Size: 191 1471
Fragments arranged by size:
1471 1394 1135 870 736 681 244 219
191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6994
SmaI CCC'GGG
Cuts at: 1872 4321 1872
Size: 2449 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5098 5999 6261 820
Size: 197 926 3155 901 262 1553
Fragments arranged by size:
3155 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4785 493
Size: 4292 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6994
SphI G_CATG'C
Cuts at: 1132 4352 1132
Size: 3220 3774
SspI AAT'ATT
Cuts at: 5 52 2254 4918 5
Size: 47 2202 2664 2081
Fragments arranged by size:
2664 2202 2081 47
StuI AGG'CCT
Cuts at: 1517 2141 4167 1517
Size: 624 2026 4344
Fragments arranged by size:
4344 2026 624
StyI C'CwwG_G
Cuts at: 513 1065 2145 2283 2424 3267 3453 4433 513
Size: 552 1080 138 141 843 186 980 3074
Fragments arranged by size:
3074 1080 980 843 552 186 141 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3052 3820 5021 5038 5191 5376 5821
Size: 1255 1472 768 1201 17 153 185 445
Cuts at: 5821 325
Size: 1498
Fragments arranged by size:
1498 1472 1255 1201 768 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 3922 5240
Size: 274 80 33 51 1443 1727 218 1318
Cuts at: 5240 6952 96
Size: 1712 138
Fragments arranged by size:
1727 1712 1443 1318 274 218 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2855 2887 6954 6986 2855
Size: 32 4067 32 2863
Fragments arranged by size:
4067 2863 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6994
XbaI T'CTAG_A
Cuts at: 4330 4330
Size: 6994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2685 2685
Size: 6994
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6994
XmaI C'CCGG_G
Cuts at: 1870 4319 1870
Size: 2449 4545
XmnI GAAnn'nnTTC
Cuts at: 5123 5123
Size: 6994
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2901 4099 4802 276
Size: 53 83 186 936 1367 1198 703 2468
Fragments arranged by size:
2468 1367 1198 936 703 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI