Restriction map for
pFN21AB6288
(Circular) (Six-base) MAPSORT of: pFN21AB6288.seq Check: 3 from: 1 to: 7057
pFN21AB6288 7057 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3106 3178 3626 3106
Size: 72 448 6537
Fragments arranged by size:
6537 448 72
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4867 278
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4400 1937
Size: 2463 4594
Acc65I G'GTAC_C
Cuts at: 4378 4378
Size: 7057
AclI AA'CG_TT
Cuts at: 1563 2352 5184 5557 1563
Size: 789 2832 373 3063
Fragments arranged by size:
3063 2832 789 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2501 3122 3135 3167 3378 3587 5120 6504
Size: 869 621 13 32 211 209 1533 1384
Cuts at: 6504 6772 1632
Size: 268 1917
Fragments arranged by size:
1917 1533 1384 869 621 268 211 209
32 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6860
AflIII A'CryG_T
Cuts at: 1203 4177 5956 6933 1203
Size: 2974 1779 977 1327
Fragments arranged by size:
2974 1779 1327 977
AgeI A'CCGG_T
Cuts at: 5888 5888
Size: 7057
AhdI GACnn_n'nnGTC
Cuts at: 5786 5786
Size: 7057
AleI CACnn'nnGTG
Cuts at: 1166 3017 3497 3803 1166
Size: 1851 480 306 4420
Fragments arranged by size:
4420 1851 480 306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2182 2334 1184
Size: 998 152 5907
Fragments arranged by size:
5907 998 152
AlwNI CAG_nnn'CTG
Cuts at: 2651 3035 4800 6372 2651
Size: 384 1765 1572 3336
Fragments arranged by size:
3336 1765 1572 384
ApaLI G'TGCA_C
Cuts at: 5114 6270 5114
Size: 1156 5901
ApoI r'AATT_y
Cuts at: 1493 2538 2589 3175 3448 4352 4366 4592 4744
Size: 1045 51 586 273 904 14 226 152
Cuts at: 4744 6853 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1045 904 586 273 226 152
51 14
AseI AT'TA_AT
Cuts at: 160 1032 5611 160
Size: 872 4579 1606
Fragments arranged by size:
4579 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
AvaI C'yCGr_G
Cuts at: 1870 1943 4234 4382 1870
Size: 73 2291 148 4545
Fragments arranged by size:
4545 2291 148 73
BamHI G'GATC_C
Cuts at: 4387 4754 4387
Size: 367 6690
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3554 4378 5833 618
Size: 325 217 627 1767 824 1455 1842
Fragments arranged by size:
1842 1767 1455 824 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3026 3128 3379 4086 4376 1391
Size: 1635 102 251 707 290 4072
Fragments arranged by size:
4072 1635 707 290 251 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3495 928
Size: 971 1596 4490
Fragments arranged by size:
4490 1596 971
BbvCI CC'TCA_GC
Cuts at: 3240 3240
Size: 7057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3346 3380 5248 5282 1903
Size: 34 1409 34 1868 34 3678
Fragments arranged by size:
3678 1868 1409 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4949 6159 4949
Size: 1210 5847
BclI T'GATC_A
Cuts at: 1548 3835 4162 1548
Size: 2287 327 4443
Fragments arranged by size:
4443 2287 327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5668 136
Size: 107 122 71 631 605 3996 1525
Fragments arranged by size:
3996 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7052 7052
Size: 7057
BlpI GC'TnA_GC
Cuts at: 3025 4474 3025
Size: 1449 5608
Bme1580I G_kGCm'C
Cuts at: 1790 3559 5118 6274 1790
Size: 1769 1559 1156 2573
Fragments arranged by size:
2573 1769 1559 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2853 3491 4788
Size: 416 53 83 399 24 1436 638 1297
Cuts at: 4788 5746 442
Size: 958 1753
Fragments arranged by size:
1753 1436 1297 958 638 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7057
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2427 2427
Size: 7057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3112 3269 4041 4070 5717 1239
Size: 1873 157 772 29 1647 2579
Fragments arranged by size:
2579 1873 1647 772 157 29
Bpu10I CC'TnA_GC
Cuts at: 3240 3240
Size: 7057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2742 4290 5182 6047 6345 2742
Size: 1548 892 865 298 3454
Fragments arranged by size:
3454 1548 892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5720 882
Size: 617 4221 2219
Fragments arranged by size:
4221 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4848 493
Size: 711 3644 2702
Fragments arranged by size:
3644 2702 711
BsaBI GATnn'nnATC
Cuts at: 3227 4314 7051 3227
Size: 1087 2737 3233
Fragments arranged by size:
3233 2737 1087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4864 5246 5881 275
Size: 53 83 186 936 3331 382 635 1451
Fragments arranged by size:
3331 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2692 5490 5888 6162 6309 6720 2692
Size: 2798 398 274 147 411 3029
Fragments arranged by size:
3029 2798 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3645 3675 3696 3715 3726
Size: 30 165 30 2242 30 21 19 11
Cuts at: 3726 3745 1178
Size: 19 4490
Fragments arranged by size:
4490 2242 165 30 30 30 21 19
19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2042 2045 2048 2133 2319 2805 2850
Size: 853 3 3 3 85 186 486 45
Cuts at: 2850 2950 2953 1186
Size: 100 3 5290
Fragments arranged by size:
5290 853 486 186 100 85 45 3
3 3 3
BseYI C'CCAG_C
Cuts at: 3467 3579 6260 6756 3467
Size: 112 2681 496 3768
Fragments arranged by size:
3768 2681 496 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2959 3668 1163
Size: 573 1223 709 4552
Fragments arranged by size:
4552 1223 709 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4507 5268 6296 1540
Size: 275 186 2506 761 1028 2301
Fragments arranged by size:
2506 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3026 3128 4003 4086 5118 5203 6274 3026
Size: 102 875 83 1032 85 1071 3809
Fragments arranged by size:
3809 1071 1032 875 102 85 83
BsmI GAATG_Cn'
Cuts at: 3156 3163 3469 4133 4568 4661 3156
Size: 7 306 664 435 93 5552
Fragments arranged by size:
5552 664 435 306 93 7
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3026 3128 3379 3559 4003 4086 4376
Size: 399 1236 102 251 180 444 83 290
Cuts at: 4376 5118 5203 6274 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1236 1071 742 444 399 290 251
180 102 85 83
BspEI T'CCGG_A
Cuts at: 6720 6720
Size: 7057
BspHI T'CATG_A
Cuts at: 4946 6676 4946
Size: 1730 5327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2597 2839 2981 3106 3178 3626 3671 4412
Size: 1753 242 142 125 72 448 45 741
Cuts at: 4412 844
Size: 3489
Fragments arranged by size:
3489 1753 741 448 242 142 125 72
45
BsrBI CCG'CTC
Cuts at: 1394 1823 4944 1394
Size: 429 3121 3507
Fragments arranged by size:
3507 3121 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5552 5726 65
Size: 1183 4304 174 1396
Fragments arranged by size:
4304 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5701 5888 1700
Size: 110 3891 187 2869
Fragments arranged by size:
3891 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2491 3418 96
Size: 2395 927 3735
Fragments arranged by size:
3735 2395 927
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7057
BssSI C'ACGA_G
Cuts at: 5117 6129 5117
Size: 1012 6045
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7057
BstBI TT'CG_AA
Cuts at: 3865 4313 5861 3865
Size: 448 1548 5061
Fragments arranged by size:
5061 1548 448
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7057
BstYI r'GATC_y
Cuts at: 1972 2227 3222 3228 4387 4754 5139 5156 6608
Size: 255 995 6 1159 367 385 17 1452
Cuts at: 6608 6694 6706 7052 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1159 995 385 367 346 255
86 17 12 6
Bsu36I CC'TnA_GG
Cuts at: 2116 2116
Size: 7057
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2069 487
Size: 683 56 843 5475
Fragments arranged by size:
5475 843 683 56
BtsI GCAGTG_nn'
Cuts at: 2723 2982 3013 4573 5367 5387 2723
Size: 259 31 1560 794 20 4393
Fragments arranged by size:
4393 1560 794 259 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7057
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7022
DraI TTT'AAA
Cuts at: 4361 4361
Size: 7057
DraIII CAC_nnn'GTG
Cuts at: 4076 4076
Size: 7057
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6064 809
Size: 507 4748 1802
Fragments arranged by size:
4748 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2854 3037 4504
Size: 54 1425 50 275 18 1024 183 1467
Cuts at: 4504 5393 7022 8
Size: 889 1629 43
Fragments arranged by size:
1629 1467 1425 1024 889 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4504 1537
Size: 275 2692 4090
Fragments arranged by size:
4090 2692 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2745 3186 3862 3904 4987 1353
Size: 1392 441 676 42 1083 3423
Fragments arranged by size:
3423 1392 1083 676 441 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5644 6018 6164 387
Size: 1064 138 4055 374 146 1280
Fragments arranged by size:
4055 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3024 3126 4084 3024
Size: 102 958 5997
Fragments arranged by size:
5997 958 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 3112 3122 3135 3167 3269 3378
Size: 393 869 611 10 13 32 102 109
Cuts at: 3378 3587 4041 4070 5120 5717 6504 6772 1239
Size: 209 454 29 1050 597 787 268 1524
Fragments arranged by size:
1524 1050 869 787 611 597 454 393
268 209 109 102 32 29 13 10
EcoRI G'AATT_C
Cuts at: 4352 4366 4744 4352
Size: 14 378 6665
Fragments arranged by size:
6665 378 14
FspI TGC'GCA
Cuts at: 5563 5563
Size: 7057
HaeII r_GCGC'y
Cuts at: 1678 6204 1678
Size: 4526 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3115 3147 3696 3728 3820
Size: 32 135 32 1543 32 549 32 92
Cuts at: 3820 3852 4150 4154 4182 4186 5698 5730 5772
Size: 32 298 4 28 4 1512 32 42
Cuts at: 5772 5804 1373
Size: 32 2626
Fragments arranged by size:
2626 1543 1512 549 298 135 92 42
32 32 32 32 32 32 32 28
4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2534 4401 4647 669
Size: 988 281 596 1867 246 3079
Fragments arranged by size:
3079 1867 988 596 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7057
HpaI GTT'AAC
Cuts at: 4647 4647
Size: 7057
KpnI G_GTAC'C
Cuts at: 4382 4382
Size: 7057
MfeI C'AATT_G
Cuts at: 4656 4656
Size: 7057
MluI A'CGCG_T
Cuts at: 6933 6933
Size: 7057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2576 3256 3380 3404 3730 6171 6355 1305
Size: 1271 680 124 24 326 2441 184 2007
Fragments arranged by size:
2441 2007 1271 680 326 184 124 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2856 3039 10
Size: 54 1425 343 1024 183 4028
Fragments arranged by size:
4028 1425 1024 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 3497 3803 4110 5015 5374 5533
Size: 648 1851 480 306 307 905 359 159
Cuts at: 5533 518
Size: 2042
Fragments arranged by size:
2042 1851 905 648 480 359 307 306
159
MspA1I CmG'CkG
Cuts at: 1606 2045 2051 2633 4473 5150 6298 6543 1606
Size: 439 6 582 1840 677 1148 245 2120
Fragments arranged by size:
2120 1840 1148 677 582 439 245 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6505
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7057
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7057
NotI GC'GGCC_GC
Cuts at: 4504 4504
Size: 7057
NspI r_CATG'y
Cuts at: 1132 1229 4181 4415 5960 1132
Size: 97 2952 234 1545 2229
Fragments arranged by size:
2952 2229 1545 234 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7057
PciI A'CATG_T
Cuts at: 4177 5956 4177
Size: 1779 5278
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7057
PmeI GTTT'AAAC
Cuts at: 4361 4361
Size: 7057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5126 5158 6639 6671 5126
Size: 32 1481 32 5512
Fragments arranged by size:
5512 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7057
PsiI TTA'TAA
Cuts at: 4627 4627
Size: 7057
PstI C_TGCA'G
Cuts at: 830 2608 2730 3637 4409 830
Size: 1778 122 907 772 3478
Fragments arranged by size:
3478 1778 907 772 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7057
PvuII CAG'CTG
Cuts at: 2633 2633
Size: 7057
SacI G_AGCT'C
Cuts at: 3026 3128 4086 3026
Size: 102 958 5997
Fragments arranged by size:
5997 958 102
SalI G'TCGA_C
Cuts at: 1936 4399 1936
Size: 2463 4594
SbfI CC_TGCA'GG
Cuts at: 4409 4409
Size: 7057
ScaI AGT'ACT
Cuts at: 2941 5305 7017 2941
Size: 2364 1712 2981
Fragments arranged by size:
2981 2364 1712
SexAI A'CCwGG_T
Cuts at: 2707 2707
Size: 7057
SfcI C'TryA_G
Cuts at: 826 1045 2061 2570 2604 2726 3633 3726 3741
Size: 219 1016 509 34 122 907 93 15
Cuts at: 3741 4405 5540 6221 6412 826
Size: 664 1135 681 191 1471
Fragments arranged by size:
1471 1135 1016 907 681 664 509 219
191 122 93 34 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7057
SmaI CCC'GGG
Cuts at: 1872 4384 1872
Size: 2512 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2757 4305 5161 6062 6324 820
Size: 197 926 814 1548 856 901 262 1553
Fragments arranged by size:
1553 1548 926 901 856 814 262 197
SnaBI TAC'GTA
Cuts at: 493 4848 493
Size: 4355 2702
SpeI A'CTAG_T
Cuts at: 152 2555 3671 152
Size: 2403 1116 3538
Fragments arranged by size:
3538 2403 1116
SphI G_CATG'C
Cuts at: 1132 4415 1132
Size: 3283 3774
SspI AAT'ATT
Cuts at: 5 52 2659 3433 4981 5
Size: 47 2607 774 1548 2081
Fragments arranged by size:
2607 2081 1548 774 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7057
StyI C'CwwG_G
Cuts at: 513 1065 4496 513
Size: 552 3431 3074
Fragments arranged by size:
3431 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2929 3617 5084 5101 5254 5439 5884
Size: 1255 1349 688 1467 17 153 185 445
Cuts at: 5884 325
Size: 1498
Fragments arranged by size:
1498 1467 1349 1255 688 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2470 2491 2939
Size: 274 80 33 51 1443 493 21 448
Cuts at: 2939 3418 5303 7015 96
Size: 479 1885 1712 138
Fragments arranged by size:
1885 1712 1443 493 479 448 274 138
80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2173 2205 4039 4071 7017 7049 2173
Size: 32 1834 32 2946 32 2181
Fragments arranged by size:
2946 2181 1834 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7057
XbaI T'CTAG_A
Cuts at: 4393 4393
Size: 7057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2705 2705
Size: 7057
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7057
XmaI C'CCGG_G
Cuts at: 1870 4382 1870
Size: 2512 4545
XmnI GAAnn'nnTTC
Cuts at: 5186 5186
Size: 7057
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4865 276
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI