Restriction map for
pFN21AB6284
(Circular) (Six-base) MAPSORT of: pFN21AB6284.seq Check: 2481 from: 1 to: 7054
pFN21AB6284 7054 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4864 278
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4397 1937
Size: 2460 4594
Acc65I G'GTAC_C
Cuts at: 2745 3372 4375 2745
Size: 627 1003 5424
Fragments arranged by size:
5424 1003 627
AclI AA'CG_TT
Cuts at: 1563 5181 5554 1563
Size: 3618 373 3063
Fragments arranged by size:
3618 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 2684 5117 6501 6769 1632
Size: 956 96 2433 1384 268 1917
Fragments arranged by size:
2433 1917 1384 956 268 96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6857
AflIII A'CryG_T
Cuts at: 1203 5953 6930 1203
Size: 4750 977 1327
Fragments arranged by size:
4750 1327 977
AgeI A'CCGG_T
Cuts at: 5885 5885
Size: 7054
AhdI GACnn_n'nnGTC
Cuts at: 5783 5783
Size: 7054
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2049 3255 3306 1184
Size: 865 1206 51 4932
Fragments arranged by size:
4932 1206 865 51
AlwNI CAG_nnn'CTG
Cuts at: 2467 2813 2980 3217 3875 3900 4207 4297 4797
Size: 346 167 237 658 25 307 90 500
Cuts at: 4797 6369 2467
Size: 1572 3152
Fragments arranged by size:
3152 1572 658 500 346 307 237 167
90 25
ApaI G_GGCC'C
Cuts at: 2350 3086 3158 3414 3859 2350
Size: 736 72 256 445 5545
Fragments arranged by size:
5545 736 445 256 72
ApaLI G'TGCA_C
Cuts at: 4025 5111 6267 4025
Size: 1086 1156 4812
Fragments arranged by size:
4812 1156 1086
ApoI r'AATT_y
Cuts at: 1493 4165 4363 4589 4741 6850 1493
Size: 2672 198 226 152 2109 1697
Fragments arranged by size:
2672 2109 1697 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 2249 5608 160
Size: 872 1217 3359 1606
Fragments arranged by size:
3359 1606 1217 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
AvaI C'yCGr_G
Cuts at: 1870 1943 2769 3192 4328 4379 1870
Size: 73 826 423 1136 51 4545
Fragments arranged by size:
4545 1136 826 423 73 51
BamHI G'GATC_C
Cuts at: 2604 2692 3550 4384 4751 2604
Size: 88 858 834 367 4907
Fragments arranged by size:
4907 858 834 367 88
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2624 2667 2745 3372 3613
Size: 325 217 627 837 43 78 627 241
Cuts at: 3613 3666 4375 5830 618
Size: 53 709 1455 1842
Fragments arranged by size:
1842 1455 837 709 627 627 325 241
217 78 53 43
BanII G_rGCy'C
Cuts at: 1391 2350 2577 3086 3158 3199 3229 3414 3859
Size: 959 227 509 72 41 30 185 445
Cuts at: 3859 3993 4373 1391
Size: 134 380 4072
Fragments arranged by size:
4072 959 509 445 380 227 185 134
72 41 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3022 928
Size: 971 1123 4960
Fragments arranged by size:
4960 1123 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3804 3838 5245 5279 1903
Size: 34 1867 34 1407 34 3678
Fragments arranged by size:
3678 1867 1407 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4946 6156 4946
Size: 1210 5844
BclI T'GATC_A
Cuts at: 1548 2637 3787 1548
Size: 1089 1150 4815
Fragments arranged by size:
4815 1150 1089
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2470 5665 136
Size: 107 122 71 631 605 798 3195 1525
Fragments arranged by size:
3195 1525 798 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7049 7049
Size: 7054
BlpI GC'TnA_GC
Cuts at: 2448 2806 4471 2448
Size: 358 1665 5031
Fragments arranged by size:
5031 1665 358
Bme1580I G_kGCm'C
Cuts at: 1790 2350 2627 3086 3158 3306 3414 3618 3851
Size: 560 277 459 72 148 108 204 233
Cuts at: 3851 3859 4029 5115 6271 1790
Size: 8 170 1086 1156 2573
Fragments arranged by size:
2573 1156 1086 560 459 277 233 204
170 148 108 72 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2968 3293 3727
Size: 416 53 83 399 24 1551 325 434
Cuts at: 3727 3875 3900 4056 4785 5743 442
Size: 148 25 156 729 958 1753
Fragments arranged by size:
1753 1551 958 729 434 416 399 325
156 148 83 53 25 24
BmtI G_CTAG'C
Cuts at: 1056 2376 1056
Size: 1320 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 7054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2217 2321 2852 3183 3363 3385 3877 4098
Size: 978 104 531 331 180 22 492 221
Cuts at: 4098 5714 1239
Size: 1616 2579
Fragments arranged by size:
2579 1616 978 531 492 331 221 180
104 22
Bpu10I CC'TnA_GC
Cuts at: 3406 3506 3864 3406
Size: 100 358 6596
Fragments arranged by size:
6596 358 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5179 6044 6342 5179
Size: 865 298 5891
Fragments arranged by size:
5891 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5717 882
Size: 617 4218 2219
Fragments arranged by size:
4218 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4845 493
Size: 711 3641 2702
Fragments arranged by size:
3641 2702 711
BsaBI GATnn'nnATC
Cuts at: 7048 7048
Size: 7054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3888 4861 5243 5878
Size: 53 83 186 936 2355 973 382 635
Cuts at: 5878 275
Size: 1451
Fragments arranged by size:
2355 1451 973 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2664 3722 5487 5885 6159 6306 6717 2664
Size: 1058 1765 398 274 147 411 3001
Fragments arranged by size:
3001 1765 1058 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2516 2546 1178
Size: 30 165 30 1113 30 5686
Fragments arranged by size:
5686 1113 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2415 2849 4185 4334 4346 1186
Size: 1229 434 1336 149 12 3894
Fragments arranged by size:
3894 1336 1229 434 149 12
BseYI C'CCAG_C
Cuts at: 2467 3228 3249 3555 3765 4030 4305 6257 6753
Size: 761 21 306 210 265 275 1952 496
Cuts at: 6753 2467
Size: 2768
Fragments arranged by size:
2768 1952 761 496 306 275 265 210
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3117 3168 3433 1163
Size: 573 1381 51 265 4784
Fragments arranged by size:
4784 1381 573 265 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4504 5265 6293 1540
Size: 275 186 2503 761 1028 2301
Fragments arranged by size:
2503 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4029 5115 5200 6271 4029
Size: 1086 85 1071 4812
Fragments arranged by size:
4812 1086 1071 85
BsmI GAATG_Cn'
Cuts at: 4565 4658 4565
Size: 93 6961
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2350 2577 2627 3086 3158 3199 3229
Size: 399 560 227 50 459 72 41 30
Cuts at: 3229 3306 3414 3618 3851 3859 3993 4029 4373
Size: 77 108 204 233 8 134 36 344
Cuts at: 4373 5115 5200 6271 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 560 459 399 344 233
227 204 134 108 85 77 72 50
41 36 30 8
BspEI T'CCGG_A
Cuts at: 6717 6717
Size: 7054
BspHI T'CATG_A
Cuts at: 4943 6673 4943
Size: 1730 5324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4409 844
Size: 3565 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4941 1394
Size: 429 3118 3507
Fragments arranged by size:
3507 3118 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2284 5549 5723 65
Size: 1183 1036 3265 174 1396
Fragments arranged by size:
3265 1396 1183 1036 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3663 5698 5885 1700
Size: 110 1853 2035 187 2869
Fragments arranged by size:
2869 2035 1853 187 110
BsrGI T'GTAC_A
Cuts at: 96 2532 96
Size: 2436 4618
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7054
BssSI C'ACGA_G
Cuts at: 5114 6126 5114
Size: 1012 6042
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2667 4272 794
Size: 1873 1605 3576
Fragments arranged by size:
3576 1873 1605
BstBI TT'CG_AA
Cuts at: 5858 5858
Size: 7054
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7054
BstXI CCAn_nnnn'nTGG
Cuts at: 2830 3698 2830
Size: 868 6186
BstYI r'GATC_y
Cuts at: 1972 2604 2692 3338 3550 3803 4384 4751 5136
Size: 632 88 646 212 253 581 367 385
Cuts at: 5136 5153 6605 6691 6703 7049 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 646 632 581 385 367 346
253 212 88 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3475 513
Size: 552 2410 4092
Fragments arranged by size:
4092 2410 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6315
Fragments arranged by size:
6315 683 56
BtsI GCAGTG_nn'
Cuts at: 3105 3340 3373 3517 4285 4570 5364 5384 3105
Size: 235 33 144 768 285 794 20 4775
Fragments arranged by size:
4775 794 768 285 235 144 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7019
DraI TTT'AAA
Cuts at: 4358 4358
Size: 7054
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3440 6061 809
Size: 507 2124 2621 1802
Fragments arranged by size:
2621 2124 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2480 2585
Size: 54 1425 50 275 18 184 466 105
Cuts at: 2585 2733 2780 3043 4501 5390 7019 8
Size: 148 47 263 1458 889 1629 43
Fragments arranged by size:
1629 1458 1425 889 466 275 263 184
148 105 54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4501 1537
Size: 275 2689 4090
Fragments arranged by size:
4090 2689 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3430 4984 1353
Size: 2077 1554 3423
Fragments arranged by size:
3423 2077 1554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5641 6015 6161 387
Size: 1064 138 4052 374 146 1280
Fragments arranged by size:
4052 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2321 2588 2684 2852 3183 3363
Size: 393 585 104 267 96 168 331 180
Cuts at: 3363 3385 3877 4098 5117 5714 6501 6769 1239
Size: 22 492 221 1019 597 787 268 1524
Fragments arranged by size:
1524 1019 787 597 585 492 393 331
268 267 221 180 168 104 96 22
EcoO109I rG'GnC_Cy
Cuts at: 2347 2984 3083 3155 3321 3410 3411 3855 3856
Size: 637 99 72 166 89 1 444 1
Cuts at: 3856 3980 4151 4232 2347
Size: 124 171 81 5169
Fragments arranged by size:
5169 637 444 171 166 124 99 89
81 72 1 1
EcoRI G'AATT_C
Cuts at: 4363 4741 4363
Size: 378 6676
FspI TGC'GCA
Cuts at: 2660 5560 2660
Size: 2900 4154
HaeII r_GCGC'y
Cuts at: 1678 3217 6201 1678
Size: 1539 2984 2531
Fragments arranged by size:
2984 2531 1539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3009 3041 3389 3421 5695
Size: 32 135 32 1437 32 348 32 2274
Cuts at: 5695 5727 5769 5801 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2274 1437 348 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3445 3886 4398 4644 669
Size: 988 281 1507 441 512 246 3079
Fragments arranged by size:
3079 1507 988 512 441 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7054
HpaI GTT'AAC
Cuts at: 4644 4644
Size: 7054
KpnI G_GTAC'C
Cuts at: 2749 3376 4379 2749
Size: 627 1003 5424
Fragments arranged by size:
5424 1003 627
MfeI C'AATT_G
Cuts at: 4653 4653
Size: 7054
MluI A'CGCG_T
Cuts at: 6930 6930
Size: 7054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2310 3831 6168 6352 1305
Size: 1005 1521 2337 184 2007
Fragments arranged by size:
2337 2007 1521 1005 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2016 2482 2587 2735 2782
Size: 54 1425 343 184 466 105 148 47
Cuts at: 2782 10
Size: 4282
Fragments arranged by size:
4282 1425 466 343 184 148 105 54
47
MslI CAynn'nnrTG
Cuts at: 518 1166 2633 3237 5012 5371 5530 518
Size: 648 1467 604 1775 359 159 2042
Fragments arranged by size:
2042 1775 1467 648 604 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2029 2108 2266 2455 2467 2731 2757
Size: 417 6 79 158 189 12 264 26
Cuts at: 2757 2799 4470 5147 6295 6540 1606
Size: 42 1671 677 1148 245 2120
Fragments arranged by size:
2120 1671 1148 677 417 264 245 189
158 79 42 26 12 6
NcoI C'CATG_G
Cuts at: 513 1065 3475 513
Size: 552 2410 4092
Fragments arranged by size:
4092 2410 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7054
NheI G'CTAG_C
Cuts at: 1052 2372 1052
Size: 1320 5734
NotI GC'GGCC_GC
Cuts at: 4501 4501
Size: 7054
NspI r_CATG'y
Cuts at: 1132 1229 2944 3571 4412 5957 1132
Size: 97 1715 627 841 1545 2229
Fragments arranged by size:
2229 1715 1545 841 627 97
PasI CC'CwG_GG
Cuts at: 1348 2980 3852 1348
Size: 1632 872 4550
Fragments arranged by size:
4550 1632 872
PciI A'CATG_T
Cuts at: 5953 5953
Size: 7054
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2980 3119 3562 3875 4207 1108
Size: 1872 139 443 313 332 3955
Fragments arranged by size:
3955 1872 443 332 313 139
PfoI T'CCnGG_A
Cuts at: 3089 3199 3089
Size: 110 6944
PmeI GTTT'AAAC
Cuts at: 4358 4358
Size: 7054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5123 5155 6636 6668 5123
Size: 32 1481 32 5509
Fragments arranged by size:
5509 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2984 3980 2984
Size: 996 6058
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7054
PsiI TTA'TAA
Cuts at: 4624 4624
Size: 7054
PspOMI G'GGCC_C
Cuts at: 2346 3082 3154 3410 3855 2346
Size: 736 72 256 445 5545
Fragments arranged by size:
5545 736 445 256 72
PspXI vC'TCGA_Gb
Cuts at: 4328 4328
Size: 7054
PstI C_TGCA'G
Cuts at: 830 2028 2070 2460 2762 2804 3500 3539 4406
Size: 1198 42 390 302 42 696 39 867
Cuts at: 4406 830
Size: 3478
Fragments arranged by size:
3478 1198 867 696 390 302 42 42
39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7054
PvuII CAG'CTG
Cuts at: 2023 2029 2455 2731 2757 2799 2023
Size: 6 426 276 26 42 6278
Fragments arranged by size:
6278 426 276 42 26 6
SalI G'TCGA_C
Cuts at: 1936 4396 1936
Size: 2460 4594
SanDI GG'GwC_CC
Cuts at: 2984 3980 2984
Size: 996 6058
SapI GCTCTTCn'nnn_
Cuts at: 3430 3430
Size: 7054
SbfI CC_TGCA'GG
Cuts at: 2070 4406 2070
Size: 2336 4718
ScaI AGT'ACT
Cuts at: 5302 7014 5302
Size: 1712 5342
SexAI A'CCwGG_T
Cuts at: 3007 3118 3007
Size: 111 6943
SfcI C'TryA_G
Cuts at: 826 1045 2024 2066 2456 2758 2800 3464 3496
Size: 219 979 42 390 302 42 664 32
Cuts at: 3496 3535 4402 5537 6218 6409 826
Size: 39 867 1135 681 191 1471
Fragments arranged by size:
1471 1135 979 867 681 664 390 302
219 191 42 42 39 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7054
SmaI CCC'GGG
Cuts at: 1872 2771 3194 4381 1872
Size: 899 423 1187 4545
Fragments arranged by size:
4545 1187 899 423
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4328 5158 6059 6321 820
Size: 197 926 2385 830 901 262 1553
Fragments arranged by size:
2385 1553 926 901 830 262 197
SnaBI TAC'GTA
Cuts at: 493 4845 493
Size: 4352 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7054
SphI G_CATG'C
Cuts at: 1132 4412 1132
Size: 3280 3774
SspI AAT'ATT
Cuts at: 5 52 4978 5
Size: 47 4926 2081
Fragments arranged by size:
4926 2081 47
StuI AGG'CCT
Cuts at: 1517 3742 4119 1517
Size: 2225 377 4452
Fragments arranged by size:
4452 2225 377
StyI C'CwwG_G
Cuts at: 513 1065 3475 3600 4076 4493 513
Size: 552 2410 125 476 417 3074
Fragments arranged by size:
3074 2410 552 476 417 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3972 4044 5081 5098 5251 5436 5881
Size: 1255 2392 72 1037 17 153 185 445
Cuts at: 5881 325
Size: 1498
Fragments arranged by size:
2392 1498 1255 1037 445 185 153 72
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 3531 5300
Size: 274 80 33 51 1443 555 999 1769
Cuts at: 5300 7012 96
Size: 1712 138
Fragments arranged by size:
1769 1712 1443 999 555 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2409 3254 3286 7014 7046 2377
Size: 32 845 32 3728 32 2385
Fragments arranged by size:
3728 2385 845 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3120 1316
Size: 1804 5250
XbaI T'CTAG_A
Cuts at: 4390 4390
Size: 7054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 3436 2728
Size: 708 6346
XhoI C'TCGA_G
Cuts at: 1943 4328 1943
Size: 2385 4669
XmaI C'CCGG_G
Cuts at: 1870 2769 3192 4379 1870
Size: 899 423 1187 4545
Fragments arranged by size:
4545 1187 899 423
XmnI GAAnn'nnTTC
Cuts at: 5183 5183
Size: 7054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4862 276
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PsrI RsrII SacI SacII
SfiI SfoI SrfI SwaI