Restriction map for
pFN21AB6282
(Circular) (Six-base) MAPSORT of: pFN21AB6282.seq Check: 9641 from: 1 to: 7048
pFN21AB6282 7048 bp
With 182 enzymes: SgfI *
March 28, 2012 12:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2152
Size: 7048
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4858 278
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4120 4391 1937
Size: 2183 271 4594
Fragments arranged by size:
4594 2183 271
Acc65I G'GTAC_C
Cuts at: 4369 4369
Size: 7048
AclI AA'CG_TT
Cuts at: 1563 5175 5548 1563
Size: 3612 373 3063
Fragments arranged by size:
3612 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3294 5111 6495 6763 1632
Size: 1662 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1662 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3539 820
Size: 197 2522 4329
Fragments arranged by size:
4329 2522 197
AflIII A'CryG_T
Cuts at: 1203 5947 6924 1203
Size: 4744 977 1327
Fragments arranged by size:
4744 1327 977
AgeI A'CCGG_T
Cuts at: 5879 5879
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 5777 5777
Size: 7048
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7048
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7048
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2097
Size: 32 7016
AlwNI CAG_nnn'CTG
Cuts at: 4791 6363 4791
Size: 1572 5476
ApaI G_GGCC'C
Cuts at: 2195 2461 2195
Size: 266 6782
ApaLI G'TGCA_C
Cuts at: 5105 6261 5105
Size: 1156 5892
ApoI r'AATT_y
Cuts at: 1493 2771 2803 2966 2985 2998 3422 3593 3704
Size: 1278 32 163 19 13 424 171 111
Cuts at: 3704 4141 4226 4357 4583 4735 6844 1493
Size: 437 85 131 226 152 2109 1697
Fragments arranged by size:
2109 1697 1278 437 424 226 171 163
152 131 111 85 32 19 13
AseI AT'TA_AT
Cuts at: 160 1032 5602 160
Size: 872 4570 1606
Fragments arranged by size:
4570 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 2116 4373 1870
Size: 73 173 2257 4545
Fragments arranged by size:
4545 2257 173 73
BamHI G'GATC_C
Cuts at: 3715 4378 4745 3715
Size: 663 367 6018
Fragments arranged by size:
6018 663 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2095 4369 5824 618
Size: 325 217 627 279 29 2274 1455 1842
Fragments arranged by size:
2274 1842 1455 627 325 279 217 29
BanII G_rGCy'C
Cuts at: 1391 2195 2225 2461 4367 1391
Size: 804 30 236 1906 4072
Fragments arranged by size:
4072 1906 804 236 30
BbeI G_GCGC'C
Cuts at: 2070 2099 2070
Size: 29 7019
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2343 3247 928
Size: 971 444 904 4729
Fragments arranged by size:
4729 971 904 444
BbvCI CC'TCA_GC
Cuts at: 2613 2613
Size: 7048
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5239 5273 1903
Size: 34 3302 34 3678
Fragments arranged by size:
3678 3302 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3894 4940 6150 3894
Size: 1046 1210 4792
Fragments arranged by size:
4792 1210 1046
BclI T'GATC_A
Cuts at: 1548 2338 1548
Size: 790 6258
BfrBI ATG'CAT
Cuts at: 3058 3483 3058
Size: 425 6623
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 5659 136
Size: 107 122 71 631 605 527 3460 1525
Fragments arranged by size:
3460 1525 631 605 527 122 107 71
BglII A'GATC_T
Cuts at: 2734 7043 2734
Size: 4309 2739
BlpI GC'TnA_GC
Cuts at: 2085 4465 2085
Size: 2380 4668
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2461 5109 6265 1790
Size: 405 266 2648 1156 2573
Fragments arranged by size:
2648 2573 1156 405 266
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4779 5737 442
Size: 416 53 83 399 24 3362 958 1753
Fragments arranged by size:
3362 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 7048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 5708 1239
Size: 927 3542 2579
Fragments arranged by size:
3542 2579 927
Bpu10I CC'TnA_GC
Cuts at: 2613 2613
Size: 7048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5173 6038 6336 5173
Size: 865 298 5885
Fragments arranged by size:
5885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5711 882
Size: 617 4212 2219
Fragments arranged by size:
4212 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2893 4839 493
Size: 711 1689 1946 2702
Fragments arranged by size:
2702 1946 1689 711
BsaBI GATnn'nnATC
Cuts at: 3531 7042 3531
Size: 3511 3537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2096 4855 5237
Size: 53 83 186 936 534 29 2759 382
Cuts at: 5237 5872 275
Size: 635 1451
Fragments arranged by size:
2759 1451 936 635 534 382 186 83
53 29
BsaWI w'CCGG_w
Cuts at: 2110 2181 5481 5879 6153 6300 6711 2110
Size: 71 3300 398 274 147 411 2447
Fragments arranged by size:
3300 2447 411 398 274 147 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6823
Fragments arranged by size:
6823 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2163 2311 2488 1186
Size: 901 76 148 177 5746
Fragments arranged by size:
5746 901 177 148 76
BseYI C'CCAG_C
Cuts at: 6251 6747 6251
Size: 496 6552
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4188 1163
Size: 573 2452 4023
Fragments arranged by size:
4023 2452 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4498 5259 6287 1540
Size: 275 186 2497 761 1028 2301
Fragments arranged by size:
2497 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2225 5109 5194 6265 2225
Size: 2884 85 1071 3008
Fragments arranged by size:
3008 2884 1071 85
BsmI GAATG_Cn'
Cuts at: 4559 4652 4559
Size: 93 6955
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2225 2461 4367 5109 5194 6265
Size: 399 405 30 236 1906 742 85 1071
Cuts at: 6265 1391
Size: 2174
Fragments arranged by size:
2174 1906 1071 742 405 399 236 85
30
BspEI T'CCGG_A
Cuts at: 6711 6711
Size: 7048
BspHI T'CATG_A
Cuts at: 4937 6667 4937
Size: 1730 5318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 4403 844
Size: 1308 2251 3489
Fragments arranged by size:
3489 2251 1308
BsrBI CCG'CTC
Cuts at: 1394 1823 2865 4935 1394
Size: 429 1042 2070 3507
Fragments arranged by size:
3507 2070 1042 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3437 3754 5543 5717 65
Size: 1183 2189 317 1789 174 1396
Fragments arranged by size:
2189 1789 1396 1183 317 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5692 5879 1700
Size: 110 3882 187 2869
Fragments arranged by size:
3882 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2485 96
Size: 2389 4659
BssHII G'CGCG_C
Cuts at: 1925 2038 2157 1925
Size: 113 119 6816
Fragments arranged by size:
6816 119 113
BssSI C'ACGA_G
Cuts at: 2196 5108 6120 2196
Size: 2912 1012 3124
Fragments arranged by size:
3124 2912 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7048
BstBI TT'CG_AA
Cuts at: 5852 5852
Size: 7048
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7048
BstXI CCAn_nnnn'nTGG
Cuts at: 4131 4131
Size: 7048
BstYI r'GATC_y
Cuts at: 1972 2403 2734 2753 3715 4378 4745 5130 5147
Size: 431 331 19 962 663 367 385 17
Cuts at: 5147 6599 6685 6697 7043 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 962 663 431 385 367 346
331 86 19 17 12
Bsu36I CC'TnA_GG
Cuts at: 2873 2873
Size: 7048
BtgI C'CryG_G
Cuts at: 513 1065 2004 3572 513
Size: 552 939 1568 3989
Fragments arranged by size:
3989 1568 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2076 487
Size: 683 56 850 5459
Fragments arranged by size:
5459 850 683 56
BtsI GCAGTG_nn'
Cuts at: 2755 3613 4564 5358 5378 2755
Size: 858 951 794 20 4425
Fragments arranged by size:
4425 951 858 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7013
DraI TTT'AAA
Cuts at: 3072 3498 3561 4352 3072
Size: 426 63 791 5768
Fragments arranged by size:
5768 791 426 63
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6055 809
Size: 507 4739 1802
Fragments arranged by size:
4739 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2451 4495
Size: 54 1425 50 275 18 370 251 2044
Cuts at: 4495 5384 7013 8
Size: 889 1629 43
Fragments arranged by size:
2044 1629 1425 889 370 275 251 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4495 1537
Size: 275 2683 4090
Fragments arranged by size:
4090 2683 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2295 2510 3207 3968 4978 1353
Size: 774 168 215 697 761 1010 3423
Fragments arranged by size:
3423 1010 774 761 697 215 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2784 5635 6009 6155 387
Size: 1064 138 1195 2851 374 146 1280
Fragments arranged by size:
2851 1280 1195 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2223 2223
Size: 7048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 3294 5111 5708 6495 6763 1239
Size: 393 534 1128 1817 597 787 268 1524
Fragments arranged by size:
1817 1524 1128 787 597 534 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2191 2192 2457 2191
Size: 1 265 6782
Fragments arranged by size:
6782 265 1
EcoRI G'AATT_C
Cuts at: 3704 4226 4357 4735 3704
Size: 522 131 378 6017
Fragments arranged by size:
6017 522 378 131
FspI TGC'GCA
Cuts at: 5554 5554
Size: 7048
HaeII r_GCGC'y
Cuts at: 1678 2070 2099 6195 1678
Size: 392 29 4096 2531
Fragments arranged by size:
4096 2531 392 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 2927 2959 3146
Size: 32 135 32 677 32 646 32 187
Cuts at: 3146 3178 3206 3208 3238 3240 3707 3739 5689
Size: 32 28 2 30 2 467 32 1950
Cuts at: 5689 5721 5763 5795 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1950 677 646 467 187 135 42
32 32 32 32 32 32 32 32
30 28 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3726 4392 4638 669
Size: 988 281 1788 666 246 3079
Fragments arranged by size:
3079 1788 988 666 281 246
HindIII A'AGCT_T
Cuts at: 748 3465 3491 4275 748
Size: 2717 26 784 3521
Fragments arranged by size:
3521 2717 784 26
HpaI GTT'AAC
Cuts at: 4638 4638
Size: 7048
KasI G'GCGC_C
Cuts at: 2066 2095 2066
Size: 29 7019
KpnI G_GTAC'C
Cuts at: 4373 4373
Size: 7048
MfeI C'AATT_G
Cuts at: 4647 4647
Size: 7048
MluI A'CGCG_T
Cuts at: 6924 6924
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3525 6162 6346 1305
Size: 2220 2637 184 2007
Fragments arranged by size:
2637 2220 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 10
Size: 54 1425 343 621 4605
Fragments arranged by size:
4605 1425 621 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5006 5365 5524 518
Size: 648 3840 359 159 2042
Fragments arranged by size:
3840 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4464 5141 6289 6534 1606
Size: 2858 677 1148 245 2120
Fragments arranged by size:
2858 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2067 2096 2067
Size: 29 7019
NcoI C'CATG_G
Cuts at: 513 1065 2004 3572 513
Size: 552 939 1568 3989
Fragments arranged by size:
3989 1568 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 4495 4495
Size: 7048
NsiI A_TGCA'T
Cuts at: 3060 3485 3060
Size: 425 6623
NspI r_CATG'y
Cuts at: 1132 1229 3062 4119 4406 5951 1132
Size: 97 1833 1057 287 1545 2229
Fragments arranged by size:
2229 1833 1545 1057 287 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7048
PciI A'CATG_T
Cuts at: 5947 5947
Size: 7048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7048
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 6630 6662 5117
Size: 32 1481 32 5503
Fragments arranged by size:
5503 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7048
PsiI TTA'TAA
Cuts at: 2816 4618 2816
Size: 1802 5246
PspOMI G'GGCC_C
Cuts at: 2191 2457 2191
Size: 266 6782
PstI C_TGCA'G
Cuts at: 830 4400 830
Size: 3570 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
SacI G_AGCT'C
Cuts at: 2225 2225
Size: 7048
SalI G'TCGA_C
Cuts at: 1936 4390 1936
Size: 2454 4594
SapI GCTCTTCn'nnn_
Cuts at: 2127 3968 2127
Size: 1841 5207
SbfI CC_TGCA'GG
Cuts at: 4400 4400
Size: 7048
ScaI AGT'ACT
Cuts at: 5296 7008 5296
Size: 1712 5336
SexAI A'CCwGG_T
Cuts at: 4104 4104
Size: 7048
SfcI C'TryA_G
Cuts at: 826 1045 2849 3585 3897 4396 5531 6212 6403
Size: 219 1804 736 312 499 1135 681 191
Cuts at: 6403 826
Size: 1471
Fragments arranged by size:
1804 1471 1135 736 681 499 312 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2199 2199
Size: 7048
SfoI GGC'GCC
Cuts at: 2068 2097 2068
Size: 29 7019
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7048
SmaI CCC'GGG
Cuts at: 1872 2118 4375 1872
Size: 246 2257 4545
Fragments arranged by size:
4545 2257 246
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3539 5152 6053 6315 820
Size: 197 926 1596 1613 901 262 1553
Fragments arranged by size:
1613 1596 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2893 4839 493
Size: 2400 1946 2702
Fragments arranged by size:
2702 2400 1946
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7048
SphI G_CATG'C
Cuts at: 1132 3062 4406 1132
Size: 1930 1344 3774
Fragments arranged by size:
3774 1930 1344
SspI AAT'ATT
Cuts at: 5 52 3412 3973 4972 5
Size: 47 3360 561 999 2081
Fragments arranged by size:
3360 2081 999 561 47
StuI AGG'CCT
Cuts at: 1517 3954 1517
Size: 2437 4611
StyI C'CwwG_G
Cuts at: 513 1065 2004 3340 3572 4487 513
Size: 552 939 1336 232 915 3074
Fragments arranged by size:
3074 1336 939 915 552 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5075 5092 5245 5430 5875 325
Size: 1255 3495 17 153 185 445 1498
Fragments arranged by size:
3495 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2485 5294 7006
Size: 274 80 33 51 1443 508 2809 1712
Cuts at: 7006 96
Size: 138
Fragments arranged by size:
2809 1712 1443 508 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7008 7040 7008
Size: 32 7016
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7048
XbaI T'CTAG_A
Cuts at: 4217 4384 4217
Size: 167 6881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7048
XmaI C'CCGG_G
Cuts at: 1870 2116 4373 1870
Size: 246 2257 4545
Fragments arranged by size:
4545 2257 246
XmnI GAAnn'nnTTC
Cuts at: 3444 4251 5177 3444
Size: 807 926 5315
Fragments arranged by size:
5315 926 807
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4856 276
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BsiWI BsmBI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SrfI SwaI XcmI