Restriction map for
pFN21AB6278
(Circular) (Six-base) MAPSORT of: pFN21AB6278.seq Check: 2941 from: 1 to: 7048
pFN21AB6278 7048 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3500 3500
Size: 7048
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4858 278
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3604 4391 1937
Size: 1667 787 4594
Fragments arranged by size:
4594 1667 787
Acc65I G'GTAC_C
Cuts at: 3839 4369 3839
Size: 530 6518
AclI AA'CG_TT
Cuts at: 1563 3398 5175 5548 1563
Size: 1835 1777 373 3063
Fragments arranged by size:
3063 1835 1777 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 2562 3084 3092 3159 3305 3696 5111
Size: 785 145 522 8 67 146 391 1415
Cuts at: 5111 6495 6763 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1415 1384 785 522 391 268 146
145 67 8
AflII C'TTAA_G
Cuts at: 820 1017 3730 820
Size: 197 2713 4138
Fragments arranged by size:
4138 2713 197
AflIII A'CryG_T
Cuts at: 1203 5947 6924 1203
Size: 4744 977 1327
Fragments arranged by size:
4744 1327 977
AgeI A'CCGG_T
Cuts at: 5879 5879
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 5777 5777
Size: 7048
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7048
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4163 1184
Size: 2979 4069
AlwNI CAG_nnn'CTG
Cuts at: 4328 4791 6363 4328
Size: 463 1572 5013
Fragments arranged by size:
5013 1572 463
ApaI G_GGCC'C
Cuts at: 2266 2266
Size: 7048
ApaLI G'TGCA_C
Cuts at: 5105 6261 5105
Size: 1156 5892
ApoI r'AATT_y
Cuts at: 1493 2437 2613 3430 4043 4357 4583 4735 6844
Size: 944 176 817 613 314 226 152 2109
Cuts at: 6844 1493
Size: 1697
Fragments arranged by size:
2109 1697 944 817 613 314 226 176
152
AseI AT'TA_AT
Cuts at: 160 1032 2874 2964 5602 160
Size: 872 1842 90 2638 1606
Fragments arranged by size:
2638 1842 1606 872 90
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 2842 4373 1870
Size: 73 899 1531 4545
Fragments arranged by size:
4545 1531 899 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2787 2820 2787
Size: 33 7015
BamHI G'GATC_C
Cuts at: 4378 4745 4378
Size: 367 6681
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3839 4369 5824 618
Size: 325 217 627 2052 530 1455 1842
Fragments arranged by size:
2052 1842 1455 627 530 325 217
BanII G_rGCy'C
Cuts at: 1391 2266 3925 4367 1391
Size: 875 1659 442 4072
Fragments arranged by size:
4072 1659 875 442
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3911 928
Size: 971 2012 4065
Fragments arranged by size:
4065 2012 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5239 5273 1903
Size: 34 3302 34 3678
Fragments arranged by size:
3678 3302 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4940 6150 4940
Size: 1210 5838
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7048
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5659 136
Size: 107 122 71 631 605 3987 1525
Fragments arranged by size:
3987 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3103 7043 3103
Size: 3940 3108
BlpI GC'TnA_GC
Cuts at: 4465 4465
Size: 7048
Bme1580I G_kGCm'C
Cuts at: 1790 2266 5109 6265 1790
Size: 476 2843 1156 2573
Fragments arranged by size:
2843 2573 1156 476
BmgBI CAC'GTC
Cuts at: 1318 2056 1318
Size: 738 6310
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4779 5737 442
Size: 416 53 83 399 24 3362 958 1753
Fragments arranged by size:
3362 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3516 3963 5708 1239
Size: 2277 447 1745 2579
Fragments arranged by size:
2579 2277 1745 447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5173 6038 6336 5173
Size: 865 298 5885
Fragments arranged by size:
5885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5711 882
Size: 617 4212 2219
Fragments arranged by size:
4212 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4839 493
Size: 711 3635 2702
Fragments arranged by size:
3635 2702 711
BsaBI GATnn'nnATC
Cuts at: 7042 7042
Size: 7048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3473 4855 5237 5872
Size: 53 83 186 936 1940 1382 382 635
Cuts at: 5872 275
Size: 1451
Fragments arranged by size:
1940 1451 1382 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5481 5879 6153 6300 6711 5481
Size: 398 274 147 411 5818
Fragments arranged by size:
5818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2354 3514 3544 1178
Size: 30 165 30 921 30 1160 30 4682
Fragments arranged by size:
4682 1160 921 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 2255 2328 1186
Size: 1066 3 73 5906
Fragments arranged by size:
5906 1066 73 3
BseYI C'CCAG_C
Cuts at: 6251 6747 6251
Size: 496 6552
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2286 1163
Size: 573 550 5925
Fragments arranged by size:
5925 573 550
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2250 4498 5259 6287 1540
Size: 275 186 249 2248 761 1028 2301
Fragments arranged by size:
2301 2248 1028 761 275 249 186
BsiHKAI G_wGCw'C
Cuts at: 2320 3925 5109 5194 6265 2320
Size: 1605 1184 85 1071 3103
Fragments arranged by size:
3103 1605 1184 1071 85
BsmI GAATG_Cn'
Cuts at: 3631 3937 4559 4652 3631
Size: 306 622 93 6027
Fragments arranged by size:
6027 622 306 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2320 3925 4367 5109 5194 6265
Size: 399 476 54 1605 442 742 85 1071
Cuts at: 6265 1391
Size: 2174
Fragments arranged by size:
2174 1605 1071 742 476 442 399 85
54
BspEI T'CCGG_A
Cuts at: 6711 6711
Size: 7048
BspHI T'CATG_A
Cuts at: 4937 6667 4937
Size: 1730 5318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 2542 3079 3500 4403 844
Size: 1363 335 537 421 903 3489
Fragments arranged by size:
3489 1363 903 537 421 335
BsrBI CCG'CTC
Cuts at: 1394 1823 2012 2179 2947 4935 1394
Size: 429 189 167 768 1988 3507
Fragments arranged by size:
3507 1988 768 429 189 167
BsrDI GCAATG_nn'
Cuts at: 65 1248 5543 5717 65
Size: 1183 4295 174 1396
Fragments arranged by size:
4295 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5692 5879 1700
Size: 110 3882 187 2869
Fragments arranged by size:
3882 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3187 96
Size: 3091 3957
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7048
BssSI C'ACGA_G
Cuts at: 5108 6120 5108
Size: 1012 6036
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3433 794
Size: 2639 4409
BstBI TT'CG_AA
Cuts at: 5852 5852
Size: 7048
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7048
BstXI CCAn_nnnn'nTGG
Cuts at: 2328 2328
Size: 7048
BstYI r'GATC_y
Cuts at: 1972 2529 3103 4378 4745 5130 5147 6599 6685
Size: 557 574 1275 367 385 17 1452 86
Cuts at: 6685 6697 7043 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1275 574 557 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3605 3605
Size: 7048
BtgI C'CryG_G
Cuts at: 513 1065 2092 3268 513
Size: 552 1027 1176 4293
Fragments arranged by size:
4293 1176 1027 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2205 487
Size: 683 56 979 5330
Fragments arranged by size:
5330 979 683 56
BtsI GCAGTG_nn'
Cuts at: 4564 5358 5378 4564
Size: 794 20 6234
Fragments arranged by size:
6234 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7013
DraI TTT'AAA
Cuts at: 4352 4352
Size: 7048
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6055 809
Size: 507 4739 1802
Fragments arranged by size:
4739 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2190 2247
Size: 54 1425 50 275 18 259 101 57
Cuts at: 2247 4495 5384 7013 8
Size: 2248 889 1629 43
Fragments arranged by size:
2248 1629 1425 889 275 259 101 57
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2247 4495 1537
Size: 275 435 2248 4090
Fragments arranged by size:
4090 2248 435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2581 3013 3135 3672 3829 4978 1353
Size: 1228 432 122 537 157 1149 3423
Fragments arranged by size:
3423 1228 1149 537 432 157 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 5635 6009 6155 387
Size: 1064 138 2666 1380 374 146 1280
Fragments arranged by size:
2666 1380 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3923 3923
Size: 7048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2562 3084 3092 3159 3305 3516
Size: 393 785 145 522 8 67 146 211
Cuts at: 3516 3696 3963 5111 5708 6495 6763 1239
Size: 180 267 1148 597 787 268 1524
Fragments arranged by size:
1524 1148 787 785 597 522 393 268
267 211 180 146 145 67 8
EcoO109I rG'GnC_Cy
Cuts at: 2262 2263 2262
Size: 1 7047
EcoRI G'AATT_C
Cuts at: 4357 4735 4357
Size: 378 6670
EcoRV GAT'ATC
Cuts at: 4285 4285
Size: 7048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4304 4304
Size: 7048
FspI TGC'GCA
Cuts at: 5554 5554
Size: 7048
HaeII r_GCGC'y
Cuts at: 1678 6195 1678
Size: 4517 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 3898 3910 3930
Size: 32 135 32 752 32 1542 12 20
Cuts at: 3930 3942 5689 5721 5763 5795 1373
Size: 12 1747 32 42 32 2626
Fragments arranged by size:
2626 1747 1542 752 135 42 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4392 4638 669
Size: 988 281 2454 246 3079
Fragments arranged by size:
3079 2454 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7048
HpaI GTT'AAC
Cuts at: 4638 4638
Size: 7048
KpnI G_GTAC'C
Cuts at: 3843 4373 3843
Size: 530 6518
MfeI C'AATT_G
Cuts at: 2724 4647 2724
Size: 1923 5125
MluI A'CGCG_T
Cuts at: 6924 6924
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6162 6346 1305
Size: 4857 184 2007
Fragments arranged by size:
4857 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5226
Fragments arranged by size:
5226 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4032 5006 5365 5524 518
Size: 648 2866 974 359 159 2042
Fragments arranged by size:
2866 2042 974 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2150 3331 4464 5141 6289 6534 1606
Size: 544 1181 1133 677 1148 245 2120
Fragments arranged by size:
2120 1181 1148 1133 677 544 245
NcoI C'CATG_G
Cuts at: 513 1065 3268 513
Size: 552 2203 4293
Fragments arranged by size:
4293 2203 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 2247 4495 2247
Size: 2248 4800
NspI r_CATG'y
Cuts at: 1132 1229 4406 5951 1132
Size: 97 3177 1545 2229
Fragments arranged by size:
3177 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7048
PciI A'CATG_T
Cuts at: 5947 5947
Size: 7048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2696 3887 1108
Size: 1588 1191 4269
Fragments arranged by size:
4269 1588 1191
PfoI T'CCnGG_A
Cuts at: 3337 3616 3337
Size: 279 6769
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 6630 6662 5117
Size: 32 1481 32 5503
Fragments arranged by size:
5503 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7048
PsiI TTA'TAA
Cuts at: 4618 4618
Size: 7048
PspOMI G'GGCC_C
Cuts at: 2262 2262
Size: 7048
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4243 4275 4243
Size: 32 7016
PstI C_TGCA'G
Cuts at: 830 2109 2204 2869 3643 4195 4400 830
Size: 1279 95 665 774 552 205 3478
Fragments arranged by size:
3478 1279 774 665 552 205 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
PvuII CAG'CTG
Cuts at: 3331 3331
Size: 7048
SacI G_AGCT'C
Cuts at: 3925 3925
Size: 7048
SalI G'TCGA_C
Cuts at: 1936 4390 1936
Size: 2454 4594
SapI GCTCTTCn'nnn_
Cuts at: 2581 2581
Size: 7048
SbfI CC_TGCA'GG
Cuts at: 4400 4400
Size: 7048
ScaI AGT'ACT
Cuts at: 5296 7008 5296
Size: 1712 5336
SfcI C'TryA_G
Cuts at: 826 1045 2105 2200 2865 3171 3588 3639 4191
Size: 219 1060 95 665 306 417 51 552
Cuts at: 4191 4396 5531 6212 6403 826
Size: 205 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 681 665 552 417 306
219 205 191 95 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7048
SmaI CCC'GGG
Cuts at: 1872 4375 1872
Size: 2503 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3730 5152 6053 6315 820
Size: 197 926 1787 1422 901 262 1553
Fragments arranged by size:
1787 1553 1422 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4839 493
Size: 4346 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7048
SphI G_CATG'C
Cuts at: 1132 4406 1132
Size: 3274 3774
SspI AAT'ATT
Cuts at: 5 52 2457 2794 3160 4222 4972 5
Size: 47 2405 337 366 1062 750 2081
Fragments arranged by size:
2405 2081 1062 750 366 337 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7048
StyI C'CwwG_G
Cuts at: 513 1065 2049 3268 4487 513
Size: 552 984 1219 1219 3074
Fragments arranged by size:
3074 1219 1219 984 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5075 5092 5245 5430 5875 325
Size: 1255 3495 17 153 185 445 1498
Fragments arranged by size:
3495 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3038 3187 3406
Size: 274 80 33 51 1443 1061 149 219
Cuts at: 3406 3736 3972 4142 5294 7006 96
Size: 330 236 170 1152 1712 138
Fragments arranged by size:
1712 1443 1152 1061 330 274 236 219
170 149 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3249 3281 3514 3546 3961 3993 7008 7040 3249
Size: 32 233 32 415 32 3015 32 3257
Fragments arranged by size:
3257 3015 415 233 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7048
XbaI T'CTAG_A
Cuts at: 2824 4384 2824
Size: 1560 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2540 2540
Size: 7048
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7048
XmaI C'CCGG_G
Cuts at: 1870 4373 1870
Size: 2503 4545
XmnI GAAnn'nnTTC
Cuts at: 2662 5177 2662
Size: 2515 4533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4856 276
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI