Restriction map for
pFN21AB6277
(Circular) (Six-base) MAPSORT of: pFN21AB6277.seq Check: 5761 from: 1 to: 7048
pFN21AB6277 7048 bp
With 182 enzymes: SgfI *
May 6, 2010 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4858 278
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4391 1937
Size: 2454 4594
Acc65I G'GTAC_C
Cuts at: 4369 4369
Size: 7048
AclI AA'CG_TT
Cuts at: 1563 5175 5548 1563
Size: 3612 373 3063
Fragments arranged by size:
3612 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3056 4036 5111 6495 6763 1632
Size: 1424 980 1075 1384 268 1917
Fragments arranged by size:
1917 1424 1384 1075 980 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6851
AflIII A'CryG_T
Cuts at: 1203 2453 5947 6924 1203
Size: 1250 3494 977 1327
Fragments arranged by size:
3494 1327 1250 977
AgeI A'CCGG_T
Cuts at: 5879 5879
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 5777 5777
Size: 7048
AleI CACnn'nnGTG
Cuts at: 1166 4167 4236 1166
Size: 3001 69 3978
Fragments arranged by size:
3978 3001 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7048
AlwNI CAG_nnn'CTG
Cuts at: 2398 3176 3229 3974 4791 6363 2398
Size: 778 53 745 817 1572 3083
Fragments arranged by size:
3083 1572 817 778 745 53
ApaLI G'TGCA_C
Cuts at: 3630 5105 6261 3630
Size: 1475 1156 4417
Fragments arranged by size:
4417 1475 1156
ApoI r'AATT_y
Cuts at: 1493 2879 2910 3270 4357 4583 4735 6844 1493
Size: 1386 31 360 1087 226 152 2109 1697
Fragments arranged by size:
2109 1697 1386 1087 360 226 152 31
AseI AT'TA_AT
Cuts at: 160 1032 5602 160
Size: 872 4570 1606
Fragments arranged by size:
4570 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 2271 3747 4373 1870
Size: 73 328 1476 626 4545
Fragments arranged by size:
4545 1476 626 328 73
BamHI G'GATC_C
Cuts at: 4378 4745 4378
Size: 367 6681
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3596 4106 4369 5824
Size: 325 217 627 330 1479 510 263 1455
Cuts at: 5824 618
Size: 1842
Fragments arranged by size:
1842 1479 1455 627 510 330 325 263
217
BanII G_rGCy'C
Cuts at: 1391 2278 3733 4367 1391
Size: 887 1455 634 4072
Fragments arranged by size:
4072 1455 887 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3384 928
Size: 971 1485 4592
Fragments arranged by size:
4592 1485 971
BbvCI CC'TCA_GC
Cuts at: 3903 3903
Size: 7048
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5239 5273 1903
Size: 34 3302 34 3678
Fragments arranged by size:
3678 3302 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2170 3634 4940 6150 2170
Size: 1464 1306 1210 3068
Fragments arranged by size:
3068 1464 1306 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7048
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5659 136
Size: 107 122 71 631 605 3987 1525
Fragments arranged by size:
3987 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4267 7043 4267
Size: 2776 4272
BlpI GC'TnA_GC
Cuts at: 4465 4465
Size: 7048
Bme1580I G_kGCm'C
Cuts at: 1790 3634 4111 5109 6265 1790
Size: 1844 477 998 1156 2573
Fragments arranged by size:
2573 1844 1156 998 477
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4779 5737 442
Size: 416 53 83 399 24 3362 958 1753
Fragments arranged by size:
3362 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2727 4290 5708 1239
Size: 1488 1563 1418 2579
Fragments arranged by size:
2579 1563 1488 1418
Bpu10I CC'TnA_GC
Cuts at: 3870 3903 3870
Size: 33 7015
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1998 2214 5173 6038 6336 1998
Size: 216 2959 865 298 2710
Fragments arranged by size:
2959 2710 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3209 5711 882
Size: 617 1710 2502 2219
Fragments arranged by size:
2502 2219 1710 617
BsaAI yAC'GTr
Cuts at: 493 1204 2456 2603 4839 493
Size: 711 1252 147 2236 2702
Fragments arranged by size:
2702 2236 1252 711 147
BsaBI GATnn'nnATC
Cuts at: 7042 7042
Size: 7048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4855 5237 5872 275
Size: 53 83 186 936 3322 382 635 1451
Fragments arranged by size:
3322 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3725 5481 5879 6153 6300 6711 3725
Size: 1756 398 274 147 411 4062
Fragments arranged by size:
4062 1756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2116 2146 1178
Size: 30 165 30 713 30 6080
Fragments arranged by size:
6080 713 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2568 3909 1186
Size: 1382 1341 4325
Fragments arranged by size:
4325 1382 1341
BseYI C'CCAG_C
Cuts at: 2648 3645 6251 6747 2648
Size: 997 2606 496 2949
Fragments arranged by size:
2949 2606 997 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3282 3824 1163
Size: 573 1546 542 4387
Fragments arranged by size:
4387 1546 573 542
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4498 5259 6287 1540
Size: 275 186 2497 761 1028 2301
Fragments arranged by size:
2497 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2278 3406 3634 5109 5194 6265 2278
Size: 1128 228 1475 85 1071 3061
Fragments arranged by size:
3061 1475 1128 1071 228 85
BsiWI C'GTAC_G
Cuts at: 3653 3653
Size: 7048
BsmI GAATG_Cn'
Cuts at: 2712 3096 3457 4559 4652 2712
Size: 384 361 1102 93 5108
Fragments arranged by size:
5108 1102 384 361 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2278 3406 3634 3733 4111 4367 5109
Size: 399 488 1128 228 99 378 256 742
Cuts at: 5109 5194 6265 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1128 1071 742 488 399 378 256
228 99 85
BspEI T'CCGG_A
Cuts at: 6711 6711
Size: 7048
BspHI T'CATG_A
Cuts at: 4937 6667 4937
Size: 1730 5318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4403 844
Size: 3559 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4935 1394
Size: 429 3112 3507
Fragments arranged by size:
3507 3112 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2271 5543 5717 65
Size: 1183 1023 3272 174 1396
Fragments arranged by size:
3272 1396 1183 1023 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3067 5692 5879 1700
Size: 110 1257 2625 187 2869
Fragments arranged by size:
2869 2625 1257 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7048
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7048
BssSI C'ACGA_G
Cuts at: 5108 6120 5108
Size: 1012 6036
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2447 3575 794
Size: 1653 1128 4267
Fragments arranged by size:
4267 1653 1128
BstBI TT'CG_AA
Cuts at: 3547 5852 3547
Size: 2305 4743
BstEII G'GTnAC_C
Cuts at: 1184 3253 1184
Size: 2069 4979
BstXI CCAn_nnnn'nTGG
Cuts at: 3329 3513 4071 4235 3329
Size: 184 558 164 6142
Fragments arranged by size:
6142 558 184 164
BstYI r'GATC_y
Cuts at: 1972 2328 3118 4267 4378 4745 5130 5147 6599
Size: 356 790 1149 111 367 385 17 1452
Cuts at: 6599 6685 6697 7043 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1149 790 385 367 356 346
111 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2160 2160
Size: 7048
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 3660 513
Size: 552 939 39 1617 3901
Fragments arranged by size:
3901 1617 939 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6309
Fragments arranged by size:
6309 683 56
BtsI GCAGTG_nn'
Cuts at: 2826 4564 5358 5378 2826
Size: 1738 794 20 4496
Fragments arranged by size:
4496 1738 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7013
DraI TTT'AAA
Cuts at: 4352 4352
Size: 7048
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6055 809
Size: 507 4739 1802
Fragments arranged by size:
4739 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2691 4177
Size: 54 1425 50 275 18 210 651 1486
Cuts at: 4177 4225 4495 5384 7013 8
Size: 48 270 889 1629 43
Fragments arranged by size:
1629 1486 1425 889 651 275 270 210
54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4495 1537
Size: 275 2683 4090
Fragments arranged by size:
4090 2683 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 3411 4055 4978 1353
Size: 807 1251 644 923 3423
Fragments arranged by size:
3423 1251 923 807 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5635 6009 6155 387
Size: 1064 138 4046 374 146 1280
Fragments arranged by size:
4046 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2276 2276
Size: 7048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2727 3056 4036 4290 5111 5708 6495
Size: 393 1095 329 980 254 821 597 787
Cuts at: 6495 6763 1239
Size: 268 1524
Fragments arranged by size:
1524 1095 980 821 787 597 393 329
268 254
EcoO109I rG'GnC_Cy
Cuts at: 2652 2991 3853 4285 2652
Size: 339 862 432 5415
Fragments arranged by size:
5415 862 432 339
EcoRI G'AATT_C
Cuts at: 3270 4357 4735 3270
Size: 1087 378 5583
Fragments arranged by size:
5583 1087 378
EcoRV GAT'ATC
Cuts at: 4130 4130
Size: 7048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3224 3224
Size: 7048
FspI TGC'GCA
Cuts at: 5554 5554
Size: 7048
HaeII r_GCGC'y
Cuts at: 1678 3652 6195 1678
Size: 1974 2543 2531
Fragments arranged by size:
2543 2531 1974
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2260 2292 2413 2445 2550
Size: 32 135 32 688 32 121 32 105
Cuts at: 2550 2582 3187 3219 3535 3567 4030 4062 4072
Size: 32 605 32 316 32 463 32 10
Cuts at: 4072 4104 5689 5721 5763 5795 1373
Size: 32 1585 32 42 32 2626
Fragments arranged by size:
2626 1585 688 605 463 316 135 121
105 42 32 32 32 32 32 32
32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3128 4392 4638 669
Size: 988 281 1190 1264 246 3079
Fragments arranged by size:
3079 1264 1190 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2550 2970 3096 748
Size: 1802 420 126 4700
Fragments arranged by size:
4700 1802 420 126
HpaI GTT'AAC
Cuts at: 4638 4638
Size: 7048
KpnI G_GTAC'C
Cuts at: 4373 4373
Size: 7048
MfeI C'AATT_G
Cuts at: 4647 4647
Size: 7048
MluI A'CGCG_T
Cuts at: 6924 6924
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2013 3517 6162 6346 1305
Size: 708 1504 2645 184 2007
Fragments arranged by size:
2645 2007 1504 708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 2693 4179 4227 10
Size: 54 1425 343 210 651 1486 48 2831
Fragments arranged by size:
2831 1486 1425 651 343 210 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2115 2676 4167 4236 5006 5365
Size: 648 876 73 561 1491 69 770 359
Cuts at: 5365 5524 518
Size: 159 2042
Fragments arranged by size:
2042 1491 876 770 648 561 359 159
73 69
MspA1I CmG'CkG
Cuts at: 1606 2648 3266 3808 3971 4324 4464 5141 6289
Size: 1042 618 542 163 353 140 677 1148
Cuts at: 6289 6534 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1042 677 618 542 353 245
163 140
NaeI GCC'GGC
Cuts at: 3069 3069
Size: 7048
NcoI C'CATG_G
Cuts at: 513 1065 2004 2043 3660 513
Size: 552 939 39 1617 3901
Fragments arranged by size:
3901 1617 939 552 39
NdeI CA'TA_TG
Cuts at: 387 3964 387
Size: 3577 3471
NgoMIV G'CCGG_C
Cuts at: 3067 3067
Size: 7048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 4495 4495
Size: 7048
NspI r_CATG'y
Cuts at: 1132 1229 2326 2408 4280 4406 5951 1132
Size: 97 1097 82 1872 126 1545 2229
Fragments arranged by size:
2229 1872 1545 1097 126 97 82
PasI CC'CwG_GG
Cuts at: 1348 4111 1348
Size: 2763 4285
PciI A'CATG_T
Cuts at: 5947 5947
Size: 7048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2503 4256 1108
Size: 1269 126 1753 3900
Fragments arranged by size:
3900 1753 1269 126
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PmlI CAC'GTG
Cuts at: 2456 2456
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 6630 6662 5117
Size: 32 1481 32 5503
Fragments arranged by size:
5503 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2652 2991 3853 4285 2652
Size: 339 862 432 5415
Fragments arranged by size:
5415 862 432 339
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7048
PsiI TTA'TAA
Cuts at: 3300 4618 3300
Size: 1318 5730
PspXI vC'TCGA_Gb
Cuts at: 2271 2271
Size: 7048
PstI C_TGCA'G
Cuts at: 830 2521 2844 3850 3889 4400 830
Size: 1691 323 1006 39 511 3478
Fragments arranged by size:
3478 1691 1006 511 323 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
PvuII CAG'CTG
Cuts at: 3266 3808 3971 4324 3266
Size: 542 163 353 5990
Fragments arranged by size:
5990 542 353 163
SacI G_AGCT'C
Cuts at: 2278 2278
Size: 7048
SalI G'TCGA_C
Cuts at: 1936 4390 1936
Size: 2454 4594
SapI GCTCTTCn'nnn_
Cuts at: 3411 3411
Size: 7048
SbfI CC_TGCA'GG
Cuts at: 4400 4400
Size: 7048
ScaI AGT'ACT
Cuts at: 5296 7008 5296
Size: 1712 5336
SexAI A'CCwGG_T
Cuts at: 2496 4344 2496
Size: 1848 5200
SfcI C'TryA_G
Cuts at: 826 1045 2517 2840 3846 3885 4396 5531 6212
Size: 219 1472 323 1006 39 511 1135 681
Cuts at: 6212 6403 826
Size: 191 1471
Fragments arranged by size:
1472 1471 1135 1006 681 511 323 219
191 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7048
SmaI CCC'GGG
Cuts at: 1872 4375 1872
Size: 2503 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2013 2229 2271 5152 6053 6315
Size: 197 926 70 216 42 2881 901 262
Cuts at: 6315 820
Size: 1553
Fragments arranged by size:
2881 1553 926 901 262 216 197 70
42
SnaBI TAC'GTA
Cuts at: 493 2603 4839 493
Size: 2110 2236 2702
Fragments arranged by size:
2702 2236 2110
SpeI A'CTAG_T
Cuts at: 152 3610 152
Size: 3458 3590
SphI G_CATG'C
Cuts at: 1132 2326 4280 4406 1132
Size: 1194 1954 126 3774
Fragments arranged by size:
3774 1954 1194 126
SspI AAT'ATT
Cuts at: 5 52 4972 5
Size: 47 4920 2081
Fragments arranged by size:
4920 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7048
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 3208 3660 4123 4188 4487
Size: 552 939 39 1165 452 463 65 299
Cuts at: 4487 513
Size: 3074
Fragments arranged by size:
3074 1165 939 552 463 452 299 65
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3317 3516 3653 4211 5075 5092 5245
Size: 1255 1737 199 137 558 864 17 153
Cuts at: 5245 5430 5875 325
Size: 185 445 1498
Fragments arranged by size:
1737 1498 1255 864 558 445 199 185
153 137 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5294 7006 96
Size: 274 80 33 51 1443 3317 1712 138
Fragments arranged by size:
3317 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2445 2477 3491 3523 3937 3969 7008 7040 2445
Size: 32 1014 32 414 32 3039 32 2453
Fragments arranged by size:
3039 2453 1014 414 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7048
XbaI T'CTAG_A
Cuts at: 4384 4384
Size: 7048
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3510 3510
Size: 7048
XhoI C'TCGA_G
Cuts at: 1943 2271 1943
Size: 328 6720
XmaI C'CCGG_G
Cuts at: 1870 4373 1870
Size: 2503 4545
XmnI GAAnn'nnTTC
Cuts at: 2110 3274 5177 2110
Size: 1164 1903 3981
Fragments arranged by size:
3981 1903 1164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4856 276
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsmBI BstZ17I DraIII EcoNI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PspOMI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI