Restriction map for
pFN21AB6272
(Circular) (Six-base) MAPSORT of: pFN21AB6272.seq Check: 9679 from: 1 to: 7024
pFN21AB6272 7024 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4834 278
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4367 1937
Size: 2430 4594
Acc65I G'GTAC_C
Cuts at: 4345 4345
Size: 7024
AclI AA'CG_TT
Cuts at: 1563 3094 5151 5524 1563
Size: 1531 2057 373 3063
Fragments arranged by size:
3063 2057 1531 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2841 5087 6471 6739 1632
Size: 1209 2246 1384 268 1917
Fragments arranged by size:
2246 1917 1384 1209 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6827
AflIII A'CryG_T
Cuts at: 1203 5923 6900 1203
Size: 4720 977 1327
Fragments arranged by size:
4720 1327 977
AgeI A'CCGG_T
Cuts at: 5855 5855
Size: 7024
AhdI GACnn_n'nnGTC
Cuts at: 2566 5753 2566
Size: 3187 3837
AleI CACnn'nnGTG
Cuts at: 1166 2799 2979 1166
Size: 1633 180 5211
Fragments arranged by size:
5211 1633 180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7024
AlwNI CAG_nnn'CTG
Cuts at: 3072 4767 6339 3072
Size: 1695 1572 3757
Fragments arranged by size:
3757 1695 1572
ApaI G_GGCC'C
Cuts at: 3530 3530
Size: 7024
ApaLI G'TGCA_C
Cuts at: 5081 6237 5081
Size: 1156 5868
ApoI r'AATT_y
Cuts at: 1493 4333 4559 4711 6820 1493
Size: 2840 226 152 2109 1697
Fragments arranged by size:
2840 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5578 160
Size: 872 4546 1606
Fragments arranged by size:
4546 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
AvaI C'yCGr_G
Cuts at: 1870 1943 2173 4035 4349 1870
Size: 73 230 1862 314 4545
Fragments arranged by size:
4545 1862 314 230 73
BamHI G'GATC_C
Cuts at: 4354 4721 4354
Size: 367 6657
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 4345 5800 618
Size: 325 217 627 251 2307 1455 1842
Fragments arranged by size:
2307 1842 1455 627 325 251 217
BanII G_rGCy'C
Cuts at: 1391 2633 2996 3530 3614 4083 4343 1391
Size: 1242 363 534 84 469 260 4072
Fragments arranged by size:
4072 1242 534 469 363 260 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2469 3131 928
Size: 971 570 662 4821
Fragments arranged by size:
4821 971 662 570
BbvCI CC'TCA_GC
Cuts at: 3118 3118
Size: 7024
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3279 3313 5215 5249 1903
Size: 34 1342 34 1902 34 3678
Fragments arranged by size:
3678 1902 1342 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4916 6126 4916
Size: 1210 5814
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7024
BfrBI ATG'CAT
Cuts at: 2314 3565 2314
Size: 1251 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5635 136
Size: 107 122 71 631 605 3963 1525
Fragments arranged by size:
3963 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3953 7019 3953
Size: 3066 3958
BlpI GC'TnA_GC
Cuts at: 4441 4441
Size: 7024
Bme1580I G_kGCm'C
Cuts at: 1790 3530 5085 6241 1790
Size: 1740 1555 1156 2573
Fragments arranged by size:
2573 1740 1555 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7024
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2743 4755 5713
Size: 416 53 83 399 24 1326 2012 958
Cuts at: 5713 442
Size: 1753
Fragments arranged by size:
2012 1753 1326 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7024
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2167 2826 2983 3291 3294 4193 5684
Size: 803 125 659 157 308 3 899 1491
Cuts at: 5684 1239
Size: 2579
Fragments arranged by size:
2579 1491 899 803 659 308 157 125
3
Bpu10I CC'TnA_GC
Cuts at: 2476 3118 3613 2476
Size: 642 495 5887
Fragments arranged by size:
5887 642 495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4091 5149 6014 6312 4091
Size: 1058 865 298 4803
Fragments arranged by size:
4803 1058 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5687 882
Size: 617 4188 2219
Fragments arranged by size:
4188 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4815 493
Size: 711 3611 2702
Fragments arranged by size:
3611 2702 711
BsaBI GATnn'nnATC
Cuts at: 2970 7018 2970
Size: 4048 2976
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4831 5213 5848 275
Size: 53 83 186 936 3298 382 635 1451
Fragments arranged by size:
3298 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3510 3727 5457 5855 6129 6276 6687 3510
Size: 217 1730 398 274 147 411 3847
Fragments arranged by size:
3847 1730 411 398 274 217 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 1178
Size: 30 165 30 786 30 5983
Fragments arranged by size:
5983 786 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2986 3106 3354 3820 1186
Size: 850 950 120 248 466 4390
Fragments arranged by size:
4390 950 850 466 248 120
BseYI C'CCAG_C
Cuts at: 2270 6227 6723 2270
Size: 3957 496 2571
Fragments arranged by size:
3957 2571 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2118 4115 1163
Size: 573 382 1997 4072
Fragments arranged by size:
4072 1997 573 382
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4087 4474 5235 6263 1540
Size: 275 186 2086 387 761 1028 2301
Fragments arranged by size:
2301 2086 1028 761 387 275 186
BsiHKAI G_wGCw'C
Cuts at: 2996 4083 5085 5170 6241 2996
Size: 1087 1002 85 1071 3779
Fragments arranged by size:
3779 1087 1071 1002 85
BsmI GAATG_Cn'
Cuts at: 4535 4628 4535
Size: 93 6931
BsmBI CGTCTCn'nnnn_
Cuts at: 3429 4290 3429
Size: 861 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2633 2996 3530 3614 4083 4343 5085
Size: 399 843 363 534 84 469 260 742
Cuts at: 5085 5170 6241 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 843 742 534 469 399 363
260 85 84
BspEI T'CCGG_A
Cuts at: 3510 6687 3510
Size: 3177 3847
BspHI T'CATG_A
Cuts at: 4913 6643 4913
Size: 1730 5294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3885 4379 844
Size: 3041 494 3489
Fragments arranged by size:
3489 3041 494
BsrBI CCG'CTC
Cuts at: 1394 1823 4015 4911 1394
Size: 429 2192 896 3507
Fragments arranged by size:
3507 2192 896 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5519 5693 65
Size: 1183 4271 174 1396
Fragments arranged by size:
4271 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3254 3626 4274 5668 5855 1700
Size: 110 1444 372 648 1394 187 2869
Fragments arranged by size:
2869 1444 1394 648 372 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7024
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7024
BssSI C'ACGA_G
Cuts at: 5084 6096 5084
Size: 1012 6012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2882 794
Size: 2088 4936
BstBI TT'CG_AA
Cuts at: 2830 5828 2830
Size: 2998 4026
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7024
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 3167 2278
Size: 889 6135
BstYI r'GATC_y
Cuts at: 1972 3953 4354 4721 5106 5123 6575 6661 6673
Size: 1981 401 367 385 17 1452 86 12
Cuts at: 6673 7019 1972
Size: 346 1977
Fragments arranged by size:
1981 1977 1452 401 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2338 2338
Size: 7024
BtgI C'CryG_G
Cuts at: 513 1065 2805 3597 513
Size: 552 1740 792 3940
Fragments arranged by size:
3940 1740 792 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2396 487
Size: 683 56 1170 5115
Fragments arranged by size:
5115 1170 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 2880 2986 3528 4540 5334 5354 2410
Size: 470 106 542 1012 794 20 4080
Fragments arranged by size:
4080 1012 794 542 470 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6989
DraI TTT'AAA
Cuts at: 2125 4328 2125
Size: 2203 4821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6031 809
Size: 507 4715 1802
Fragments arranged by size:
4715 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2968 4023 4084
Size: 54 1425 50 275 18 1138 1055 61
Cuts at: 4084 4155 4272 4471 5360 6989 8
Size: 71 117 199 889 1629 43
Fragments arranged by size:
1629 1425 1138 1055 889 275 199 117
71 61 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4084 4471 1537
Size: 275 2272 387 4090
Fragments arranged by size:
4090 2272 387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2796 3049 3196 3714 4230 4954 1353
Size: 1443 253 147 518 516 724 3423
Fragments arranged by size:
3423 1443 724 518 516 253 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5611 5985 6131 387
Size: 1064 138 4022 374 146 1280
Fragments arranged by size:
4022 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2994 4081 2994
Size: 1087 5937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2167 2826 2841 2983 3291 3294
Size: 393 410 125 659 15 142 308 3
Cuts at: 3294 4193 5087 5684 6471 6739 1239
Size: 899 894 597 787 268 1524
Fragments arranged by size:
1524 899 894 787 659 597 410 393
308 268 142 125 15 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3792 4145 3792
Size: 353 6671
EcoO109I rG'GnC_Cy
Cuts at: 2190 2481 2560 3440 3666 3784 3976 2190
Size: 291 79 880 226 118 192 5238
Fragments arranged by size:
5238 880 291 226 192 118 79
EcoRI G'AATT_C
Cuts at: 4333 4711 4333
Size: 378 6646
EcoRV GAT'ATC
Cuts at: 3931 3931
Size: 7024
FseI GG_CCGG'CC
Cuts at: 4278 4278
Size: 7024
FspI TGC'GCA
Cuts at: 5530 5530
Size: 7024
HaeII r_GCGC'y
Cuts at: 1678 6171 1678
Size: 4493 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2567 2584 2599 3292
Size: 32 135 32 980 15 17 15 693
Cuts at: 3292 3324 3639 3671 3836 3868 3875 3907 3920
Size: 32 315 32 165 32 7 32 13
Cuts at: 3920 3952 4229 4261 5665 5697 5739 5771 1373
Size: 32 277 32 1404 32 42 32 2626
Fragments arranged by size:
2626 1404 980 693 315 277 165 135
42 32 32 32 32 32 32 32
32 32 32 17 15 15 13 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4368 4614 669
Size: 988 281 2430 246 3079
Fragments arranged by size:
3079 2430 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7024
HpaI GTT'AAC
Cuts at: 4614 4614
Size: 7024
KpnI G_GTAC'C
Cuts at: 4349 4349
Size: 7024
MfeI C'AATT_G
Cuts at: 4623 4623
Size: 7024
MluI A'CGCG_T
Cuts at: 6900 6900
Size: 7024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6138 6322 1305
Size: 4833 184 2007
Fragments arranged by size:
4833 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2970 4025 10
Size: 54 1425 343 1138 1055 3009
Fragments arranged by size:
3009 1425 1138 1055 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2705 2799 2979 3087 3165 3539 4982
Size: 648 1539 94 180 108 78 374 1443
Cuts at: 4982 5341 5500 518
Size: 359 159 2042
Fragments arranged by size:
2042 1539 1443 648 374 359 180 159
108 94 78
MspA1I CmG'CkG
Cuts at: 1606 3389 4440 5117 6265 6510 1606
Size: 1783 1051 677 1148 245 2120
Fragments arranged by size:
2120 1783 1148 1051 677 245
NaeI GCC'GGC
Cuts at: 3256 4276 3256
Size: 1020 6004
NcoI C'CATG_G
Cuts at: 513 1065 2805 3597 513
Size: 552 1740 792 3940
Fragments arranged by size:
3940 1740 792 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7024
NgoMIV G'CCGG_C
Cuts at: 3254 4274 3254
Size: 1020 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7024
NotI GC'GGCC_GC
Cuts at: 4471 4471
Size: 7024
NsiI A_TGCA'T
Cuts at: 2316 3567 2316
Size: 1251 5773
NspI r_CATG'y
Cuts at: 1132 1229 2151 3565 3802 4382 5927 1132
Size: 97 922 1414 237 580 1545 2229
Fragments arranged by size:
2229 1545 1414 922 580 237 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7024
PciI A'CATG_T
Cuts at: 5923 5923
Size: 7024
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3523 4153 4286 1108
Size: 2415 630 133 3846
Fragments arranged by size:
3846 2415 630 133
PfoI T'CCnGG_A
Cuts at: 2183 2513 2183
Size: 330 6694
PmeI GTTT'AAAC
Cuts at: 4328 4328
Size: 7024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2156 2188 2219 2251 5093 5125 6606 6638 2156
Size: 32 31 32 2842 32 1481 32 2542
Fragments arranged by size:
2842 2542 1481 32 32 32 32 31
PpuMI rG'GwC_Cy
Cuts at: 2481 2560 3440 3666 3784 3976 2481
Size: 79 880 226 118 192 5529
Fragments arranged by size:
5529 880 226 192 118 79
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7024
PsiI TTA'TAA
Cuts at: 4594 4594
Size: 7024
PspOMI G'GGCC_C
Cuts at: 3526 3526
Size: 7024
PspXI vC'TCGA_Gb
Cuts at: 2173 2173
Size: 7024
PstI C_TGCA'G
Cuts at: 830 4376 830
Size: 3546 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7024
PvuII CAG'CTG
Cuts at: 3389 3389
Size: 7024
SacI G_AGCT'C
Cuts at: 2996 4083 2996
Size: 1087 5937
SalI G'TCGA_C
Cuts at: 1936 4366 1936
Size: 2430 4594
SanDI GG'GwC_CC
Cuts at: 3666 3666
Size: 7024
SbfI CC_TGCA'GG
Cuts at: 4376 4376
Size: 7024
ScaI AGT'ACT
Cuts at: 2753 5272 6984 2753
Size: 2519 1712 2793
Fragments arranged by size:
2793 2519 1712
SexAI A'CCwGG_T
Cuts at: 2740 3720 2740
Size: 980 6044
SfcI C'TryA_G
Cuts at: 826 1045 3073 3812 4372 5507 6188 6379 826
Size: 219 2028 739 560 1135 681 191 1471
Fragments arranged by size:
2028 1471 1135 739 681 560 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7024
SmaI CCC'GGG
Cuts at: 1872 4351 1872
Size: 2479 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2173 4106 5128 6029 6291 820
Size: 197 926 230 1933 1022 901 262 1553
Fragments arranged by size:
1933 1553 1022 926 901 262 230 197
SnaBI TAC'GTA
Cuts at: 493 4815 493
Size: 4322 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7024
SphI G_CATG'C
Cuts at: 1132 3802 4382 1132
Size: 2670 580 3774
Fragments arranged by size:
3774 2670 580
SspI AAT'ATT
Cuts at: 5 52 4948 5
Size: 47 4896 2081
Fragments arranged by size:
4896 2081 47
StuI AGG'CCT
Cuts at: 1517 2788 3416 1517
Size: 1271 628 5125
Fragments arranged by size:
5125 1271 628
StyI C'CwwG_G
Cuts at: 513 1065 2193 2287 2563 2689 2805 2987 3597
Size: 552 1128 94 276 126 116 182 610
Cuts at: 3597 4269 4463 513
Size: 672 194 3074
Fragments arranged by size:
3074 1128 672 610 552 276 194 182
126 116 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3590 5051 5068 5221 5406 5851 325
Size: 1255 2010 1461 17 153 185 445 1498
Fragments arranged by size:
2010 1498 1461 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2751 5270 6982
Size: 274 80 33 51 1443 774 2519 1712
Cuts at: 6982 96
Size: 138
Fragments arranged by size:
2519 1712 1443 774 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2251 2283 3658 3690 6984 7016 2251
Size: 32 1375 32 3294 32 2259
Fragments arranged by size:
3294 2259 1375 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7024
XbaI T'CTAG_A
Cuts at: 3398 4360 3398
Size: 962 6062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 3604 3677 2191
Size: 1413 73 5538
Fragments arranged by size:
5538 1413 73
XhoI C'TCGA_G
Cuts at: 1943 2173 1943
Size: 230 6794
XmaI C'CCGG_G
Cuts at: 1870 4349 1870
Size: 2479 4545
XmnI GAAnn'nnTTC
Cuts at: 2725 5153 2725
Size: 2428 4596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4832 276
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I DraIII FalI FspAI KasI NarI NruI
PacI PmlI PsrI RsrII SacII SapI SfiI SfoI
SrfI SwaI