Restriction map for
pFN21AB6264
(Circular) (Six-base) MAPSORT of: pFN21AB6264.seq Check: 9153 from: 1 to: 7012
pFN21AB6264 7012 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2199 2199
Size: 7012
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4822 278
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2695 4355 1937
Size: 758 1660 4594
Fragments arranged by size:
4594 1660 758
Acc65I G'GTAC_C
Cuts at: 4333 4333
Size: 7012
AclI AA'CG_TT
Cuts at: 1563 5139 5512 1563
Size: 3576 373 3063
Fragments arranged by size:
3576 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2074 2238 2593 2927 2992 5075 6459 6727
Size: 442 164 355 334 65 2083 1384 268
Cuts at: 6727 1632
Size: 1917
Fragments arranged by size:
2083 1917 1384 442 355 334 268 164
65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6815
AflIII A'CryG_T
Cuts at: 1203 2956 4158 5911 6888 1203
Size: 1753 1202 1753 977 1327
Fragments arranged by size:
1753 1753 1327 1202 977
AgeI A'CCGG_T
Cuts at: 3435 5843 3435
Size: 2408 4604
AhdI GACnn_n'nnGTC
Cuts at: 5741 5741
Size: 7012
AleI CACnn'nnGTG
Cuts at: 1166 2969 3666 1166
Size: 1803 697 4512
Fragments arranged by size:
4512 1803 697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7012
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2196
Size: 32 6980
AlwNI CAG_nnn'CTG
Cuts at: 2910 3162 3834 3868 4086 4755 6327 2910
Size: 252 672 34 218 669 1572 3595
Fragments arranged by size:
3595 1572 672 669 252 218 34
ApaLI G'TGCA_C
Cuts at: 5069 6225 5069
Size: 1156 5856
ApoI r'AATT_y
Cuts at: 1493 2405 2519 2622 2706 2874 3126 3210 3378
Size: 912 114 103 84 168 252 84 168
Cuts at: 3378 3630 3714 3798 4050 4134 4321 4547 4699
Size: 252 84 84 252 84 187 226 152
Cuts at: 4699 6808 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 912 252 252 252 226 187
168 168 152 114 103 84 84 84
84 84
AseI AT'TA_AT
Cuts at: 160 1032 5566 160
Size: 872 4534 1606
Fragments arranged by size:
4534 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
AvaI C'yCGr_G
Cuts at: 1870 1943 4337 1870
Size: 73 2394 4545
Fragments arranged by size:
4545 2394 73
BamHI G'GATC_C
Cuts at: 4342 4709 4342
Size: 367 6645
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2130 4333 5788 618
Size: 325 217 627 343 2203 1455 1842
Fragments arranged by size:
2203 1842 1455 627 343 325 217
BanII G_rGCy'C
Cuts at: 1391 4331 1391
Size: 2940 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2855 2998 4016 928
Size: 971 956 143 1018 3924
Fragments arranged by size:
3924 1018 971 956 143
BbvCI CC'TCA_GC
Cuts at: 2038 2038
Size: 7012
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5203 5237 1903
Size: 34 50 34 3182 34 3678
Fragments arranged by size:
3678 3182 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2362 2385 4904 6114 2362
Size: 23 2519 1210 3260
Fragments arranged by size:
3260 2519 1210 23
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7012
BfrBI ATG'CAT
Cuts at: 2484 2782 3240 2484
Size: 298 458 6256
Fragments arranged by size:
6256 458 298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5623 136
Size: 107 122 71 631 605 3951 1525
Fragments arranged by size:
3951 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2686 7007 2686
Size: 4321 2691
BlpI GC'TnA_GC
Cuts at: 4429 4429
Size: 7012
Bme1580I G_kGCm'C
Cuts at: 1790 2263 5073 6229 1790
Size: 473 2810 1156 2573
Fragments arranged by size:
2810 2573 1156 473
BmgBI CAC'GTC
Cuts at: 1318 2282 1318
Size: 964 6048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4743 5701
Size: 416 53 83 399 24 2147 1179 958
Cuts at: 5701 442
Size: 1753
Fragments arranged by size:
2147 1753 1179 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7012
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2489 2657 2489
Size: 168 6844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2195 2736 2904 3156 3324 3660 3828
Size: 795 161 541 168 252 168 336 168
Cuts at: 3828 4080 4164 4248 5672 1239
Size: 252 84 84 1424 2579
Fragments arranged by size:
2579 1424 795 541 336 252 252 168
168 168 161 84 84
Bpu10I CC'TnA_GC
Cuts at: 2038 2038
Size: 7012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2759 5137 6002 6300 2759
Size: 2378 865 298 3471
Fragments arranged by size:
3471 2378 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 5675 882
Size: 617 672 3504 2219
Fragments arranged by size:
3504 2219 672 617
BsaAI yAC'GTr
Cuts at: 493 1204 4159 4803 493
Size: 711 2955 644 2702
Fragments arranged by size:
2955 2702 711 644
BsaBI GATnn'nnATC
Cuts at: 7006 7006
Size: 7012
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4819 5201 5836 275
Size: 53 83 186 936 3286 382 635 1451
Fragments arranged by size:
3286 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2630 3435 3974 5445 5843 6117 6264 6675 2630
Size: 805 539 1471 398 274 147 411 2967
Fragments arranged by size:
2967 1471 805 539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6787
Fragments arranged by size:
6787 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7012
BseYI C'CCAG_C
Cuts at: 6215 6711 6215
Size: 496 6516
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2923 4099 1163
Size: 573 1187 1176 4076
Fragments arranged by size:
4076 1187 1176 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4462 5223 6251 1540
Size: 275 186 2461 761 1028 2301
Fragments arranged by size:
2461 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5073 5158 6229 5073
Size: 85 1071 5856
Fragments arranged by size:
5856 1071 85
BsmI GAATG_Cn'
Cuts at: 2402 2654 2738 2753 2831 3158 3425 3578 3662
Size: 252 84 15 78 327 267 153 84
Cuts at: 3662 3830 4523 4616 2402
Size: 168 693 93 4798
Fragments arranged by size:
4798 693 327 267 252 168 153 93
84 84 78 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 4331 5073 5158 6229 1391
Size: 399 473 2068 742 85 1071 2174
Fragments arranged by size:
2174 2068 1071 742 473 399 85
BspEI T'CCGG_A
Cuts at: 6675 6675
Size: 7012
BspHI T'CATG_A
Cuts at: 4901 6631 4901
Size: 1730 5282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 3930 4367 844
Size: 1355 1731 437 3489
Fragments arranged by size:
3489 1731 1355 437
BsrBI CCG'CTC
Cuts at: 1394 1823 4899 1394
Size: 429 3076 3507
Fragments arranged by size:
3507 3076 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2575 5507 5681 65
Size: 1183 1327 2932 174 1396
Fragments arranged by size:
2932 1396 1327 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3435 5656 5843 1700
Size: 110 1625 2221 187 2869
Fragments arranged by size:
2869 2221 1625 187 110
BsrGI T'GTAC_A
Cuts at: 96 2506 96
Size: 2410 4602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7012
BssSI C'ACGA_G
Cuts at: 5072 6084 5072
Size: 1012 6000
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7012
BstBI TT'CG_AA
Cuts at: 5816 5816
Size: 7012
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7012
BstXI CCAn_nnnn'nTGG
Cuts at: 2016 3668 2016
Size: 1652 5360
BstYI r'GATC_y
Cuts at: 1972 2686 2807 4342 4709 5094 5111 6563 6649
Size: 714 121 1535 367 385 17 1452 86
Cuts at: 6649 6661 7007 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1535 1452 714 385 367 346 121
86 17 12
BstZ17I GTA'TAC
Cuts at: 2696 2696
Size: 7012
BtgI C'CryG_G
Cuts at: 513 1065 2255 513
Size: 552 1190 5270
Fragments arranged by size:
5270 1190 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6273
Fragments arranged by size:
6273 683 56
BtsI GCAGTG_nn'
Cuts at: 2261 3163 3835 4087 4528 5322 5342 2261
Size: 902 672 252 441 794 20 3931
Fragments arranged by size:
3931 902 794 672 441 252 20
ClaI AT'CG_AT
Cuts at: 1554 2106 1554
Size: 552 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2557 2592 3397 3432 3565 3600 529
Size: 35 1993 35 805 35 133 35 3941
Fragments arranged by size:
3941 1993 805 133 35 35 35 35
DraI TTT'AAA
Cuts at: 2563 4316 2563
Size: 1753 5259
DraIII CAC_nnn'GTG
Cuts at: 3750 3750
Size: 7012
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6019 809
Size: 507 4703 1802
Fragments arranged by size:
4703 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4459 5348 6977
Size: 54 1425 50 275 18 2629 889 1629
Cuts at: 6977 8
Size: 43
Fragments arranged by size:
2629 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4459 1537
Size: 275 2647 4090
Fragments arranged by size:
4090 2647 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2612 3751 4942 1353
Size: 1259 1139 1191 3423
Fragments arranged by size:
3423 1259 1191 1139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5599 5973 6119 387
Size: 1064 138 4010 374 146 1280
Fragments arranged by size:
4010 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2074 2195 2238 2593 2736 2904
Size: 393 402 40 121 43 355 143 168
Cuts at: 2904 2927 2992 3156 3324 3660 3828 4080 4164
Size: 23 65 164 168 336 168 252 84
Cuts at: 4164 4248 5075 5672 6459 6727 1239
Size: 84 827 597 787 268 1524
Fragments arranged by size:
1524 827 787 597 402 393 355 336
268 252 168 168 168 164 143 121
84 84 65 43 40 23
EcoRI G'AATT_C
Cuts at: 2622 2874 3126 3210 3378 3798 4050 4134 4321
Size: 252 252 84 168 420 252 84 187
Cuts at: 4321 4699 2622
Size: 378 4935
Fragments arranged by size:
4935 420 378 252 252 252 187 168
84 84
EcoRV GAT'ATC
Cuts at: 2207 2207
Size: 7012
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3796 3292
Size: 504 6508
FspI TGC'GCA
Cuts at: 5518 5518
Size: 7012
HaeII r_GCGC'y
Cuts at: 1678 6159 1678
Size: 4481 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2011 2043 2266 2298 2799
Size: 32 135 32 439 32 223 32 501
Cuts at: 2799 2831 3103 3135 3271 3303 3523 3555 3750
Size: 32 272 32 136 32 220 32 195
Cuts at: 3750 3782 4027 4059 5653 5685 5727 5759 1373
Size: 32 245 32 1594 32 42 32 2626
Fragments arranged by size:
2626 1594 501 439 272 245 223 220
195 136 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4356 4602 669
Size: 988 281 2418 246 3079
Fragments arranged by size:
3079 2418 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2388 748
Size: 1640 5372
HpaI GTT'AAC
Cuts at: 4602 4602
Size: 7012
KpnI G_GTAC'C
Cuts at: 4337 4337
Size: 7012
MfeI C'AATT_G
Cuts at: 4611 4611
Size: 7012
MluI A'CGCG_T
Cuts at: 6888 6888
Size: 7012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2217 3045 6126 6310 1305
Size: 912 828 3081 184 2007
Fragments arranged by size:
3081 2007 912 828 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5190
Fragments arranged by size:
5190 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2899 2955 2969 3321 3666 4970 5329
Size: 648 1733 56 14 352 345 1304 359
Cuts at: 5329 5488 518
Size: 159 2042
Fragments arranged by size:
2042 1733 1304 648 359 352 345 159
56 14
MspA1I CmG'CkG
Cuts at: 1606 4428 5105 6253 6498 1606
Size: 2822 677 1148 245 2120
Fragments arranged by size:
2822 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2255 513
Size: 552 1190 5270
Fragments arranged by size:
5270 1190 552
NdeI CA'TA_TG
Cuts at: 387 2544 2896 3068 387
Size: 2157 352 172 4331
Fragments arranged by size:
4331 2157 352 172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7012
NotI GC'GGCC_GC
Cuts at: 4459 4459
Size: 7012
NsiI A_TGCA'T
Cuts at: 2486 2784 3242 2486
Size: 298 458 6256
Fragments arranged by size:
6256 458 298
NspI r_CATG'y
Cuts at: 1132 1229 4370 5915 1132
Size: 97 3141 1545 2229
Fragments arranged by size:
3141 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7012
PciI A'CATG_T
Cuts at: 5911 5911
Size: 7012
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7012
PmeI GTTT'AAAC
Cuts at: 4316 4316
Size: 7012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5081 5113 6594 6626 5081
Size: 32 1481 32 5467
Fragments arranged by size:
5467 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4024 1613
Size: 2411 4601
PsiI TTA'TAA
Cuts at: 2343 2679 4582 2343
Size: 336 1903 4773
Fragments arranged by size:
4773 1903 336
PstI C_TGCA'G
Cuts at: 830 2268 4364 830
Size: 1438 2096 3478
Fragments arranged by size:
3478 2096 1438
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7012
SalI G'TCGA_C
Cuts at: 1936 4354 1936
Size: 2418 4594
SapI GCTCTTCn'nnn_
Cuts at: 3751 3751
Size: 7012
SbfI CC_TGCA'GG
Cuts at: 4364 4364
Size: 7012
ScaI AGT'ACT
Cuts at: 5260 6972 5260
Size: 1712 5300
SfcI C'TryA_G
Cuts at: 826 1045 2182 2264 3097 4360 5495 6176 6367
Size: 219 1137 82 833 1263 1135 681 191
Cuts at: 6367 826
Size: 1471
Fragments arranged by size:
1471 1263 1137 1135 833 681 219 191
82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7012
SmaI CCC'GGG
Cuts at: 1872 4339 1872
Size: 2467 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2774 5116 6017 6279 820
Size: 197 926 831 2342 901 262 1553
Fragments arranged by size:
2342 1553 926 901 831 262 197
SnaBI TAC'GTA
Cuts at: 493 4803 493
Size: 4310 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7012
SphI G_CATG'C
Cuts at: 1132 4370 1132
Size: 3238 3774
SspI AAT'ATT
Cuts at: 5 52 4936 5
Size: 47 4884 2081
Fragments arranged by size:
4884 2081 47
StuI AGG'CCT
Cuts at: 1517 2837 3089 3509 3929 4181 1517
Size: 1320 252 420 420 252 4348
Fragments arranged by size:
4348 1320 420 420 252 252
StyI C'CwwG_G
Cuts at: 513 1065 2255 4451 513
Size: 552 1190 2196 3074
Fragments arranged by size:
3074 2196 1190 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2438 3530 5039 5056 5209 5394 5839
Size: 1255 858 1092 1509 17 153 185 445
Cuts at: 5839 325
Size: 1498
Fragments arranged by size:
1509 1498 1255 1092 858 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2443 2506 3071
Size: 274 80 33 51 1443 466 63 565
Cuts at: 3071 5258 6970 96
Size: 2187 1712 138
Fragments arranged by size:
2187 1712 1443 565 466 274 138 80
63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6972 7004 6972
Size: 32 6980
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7012
XbaI T'CTAG_A
Cuts at: 4348 4348
Size: 7012
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7012
XmaI C'CCGG_G
Cuts at: 1870 4337 1870
Size: 2467 4545
XmnI GAAnn'nnTTC
Cuts at: 2669 3005 3173 3425 3677 4097 4265 5141 2669
Size: 336 168 252 252 420 168 876 4540
Fragments arranged by size:
4540 876 420 336 252 252 168 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4820 276
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI