Restriction map for
pFN21AB6263
(Circular) (Six-base) MAPSORT of: pFN21AB6263.seq Check: 6359 from: 1 to: 6769
pFN21AB6263 6769 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2366 3462 3789 2366
Size: 1096 327 5346
Fragments arranged by size:
5346 1096 327
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4579 278
Size: 53 83 186 936 3043 2468
Fragments arranged by size:
3043 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4112 1937
Size: 2175 4594
Acc65I G'GTAC_C
Cuts at: 4090 4090
Size: 6769
AclI AA'CG_TT
Cuts at: 1563 4896 5269 1563
Size: 3333 373 3063
Fragments arranged by size:
3333 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2343 2603 2682 3550 4832 6216 6484 1632
Size: 711 260 79 868 1282 1384 268 1917
Fragments arranged by size:
1917 1384 1282 868 711 268 260 79
AfeI AGC'GCT
Cuts at: 3590 3590
Size: 6769
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6572
AflIII A'CryG_T
Cuts at: 1203 2739 2888 3397 5668 6645 1203
Size: 1536 149 509 2271 977 1327
Fragments arranged by size:
2271 1536 1327 977 509 149
AgeI A'CCGG_T
Cuts at: 5600 5600
Size: 6769
AhdI GACnn_n'nnGTC
Cuts at: 2475 5498 2475
Size: 3023 3746
AleI CACnn'nnGTG
Cuts at: 1166 2127 3297 3402 3468 3495 1166
Size: 961 1170 105 66 27 4440
Fragments arranged by size:
4440 1170 961 105 66 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3912 1184
Size: 2728 4041
AlwNI CAG_nnn'CTG
Cuts at: 3982 4046 4512 6084 3982
Size: 64 466 1572 4667
Fragments arranged by size:
4667 1572 466 64
ApaI G_GGCC'C
Cuts at: 2400 2561 3609 2400
Size: 161 1048 5560
Fragments arranged by size:
5560 1048 161
ApaLI G'TGCA_C
Cuts at: 3104 3704 3742 4826 5982 3104
Size: 600 38 1084 1156 3891
Fragments arranged by size:
3891 1156 1084 600 38
ApoI r'AATT_y
Cuts at: 1493 4078 4304 4456 6565 1493
Size: 2585 226 152 2109 1697
Fragments arranged by size:
2585 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5323 160
Size: 872 4291 1606
Fragments arranged by size:
4291 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6769
AvaI C'yCGr_G
Cuts at: 1870 1943 2624 3340 4052 4094 1870
Size: 73 681 716 712 42 4545
Fragments arranged by size:
4545 716 712 681 73 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2853 2886 2853
Size: 33 6736
BamHI G'GATC_C
Cuts at: 4099 4466 4099
Size: 367 6402
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2666 2869 3330 4090 5545
Size: 325 217 627 879 203 461 760 1455
Cuts at: 5545 618
Size: 1842
Fragments arranged by size:
1842 1455 879 760 627 461 325 217
203
BanII G_rGCy'C
Cuts at: 1391 2052 2400 2502 2561 2828 3214 3609 3917
Size: 661 348 102 59 267 386 395 308
Cuts at: 3917 4026 4088 1391
Size: 109 62 4072
Fragments arranged by size:
4072 661 395 386 348 308 267 109
102 62 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3043 928
Size: 971 1144 4654
Fragments arranged by size:
4654 1144 971
BbvCI CC'TCA_GC
Cuts at: 2906 2906
Size: 6769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4960 4994 1903
Size: 34 3023 34 3678
Fragments arranged by size:
3678 3023 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4661 5871 4661
Size: 1210 5559
BclI T'GATC_A
Cuts at: 1548 3195 3852 1548
Size: 1647 657 4465
Fragments arranged by size:
4465 1647 657
BfrBI ATG'CAT
Cuts at: 3227 3227
Size: 6769
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2647 3351 5380
Size: 107 122 71 631 605 975 704 2029
Cuts at: 5380 136
Size: 1525
Fragments arranged by size:
2029 1525 975 704 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2217 6764 2217
Size: 4547 2222
BlpI GC'TnA_GC
Cuts at: 2805 2838 4186 2805
Size: 33 1348 5388
Fragments arranged by size:
5388 1348 33
Bme1580I G_kGCm'C
Cuts at: 1790 2400 2561 2643 2671 3108 3333 3609 3708
Size: 610 161 82 28 437 225 276 99
Cuts at: 3708 3746 4830 5986 1790
Size: 38 1084 1156 2573
Fragments arranged by size:
2573 1156 1084 610 437 276 225 161
99 82 38 28
BmgBI CAC'GTC
Cuts at: 1318 2098 2428 3709 1318
Size: 780 330 1281 4378
Fragments arranged by size:
4378 1281 780 330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2955 4500 5458
Size: 416 53 83 399 24 1538 1545 958
Cuts at: 5458 442
Size: 1753
Fragments arranged by size:
1753 1545 1538 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6769
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 3931 2578
Size: 1353 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2146 3029 3926 4040 5429 1239
Size: 907 883 897 114 1389 2579
Fragments arranged by size:
2579 1389 907 897 883 114
Bpu10I CC'TnA_GC
Cuts at: 2179 2816 2906 2179
Size: 637 90 6042
Fragments arranged by size:
6042 637 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2630 4894 5759 6057 2630
Size: 2264 865 298 3342
Fragments arranged by size:
3342 2264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5432 882
Size: 617 3933 2219
Fragments arranged by size:
3933 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3057 3400 3622 4560 493
Size: 711 1853 343 222 938 2702
Fragments arranged by size:
2702 1853 938 711 343 222
BsaBI GATnn'nnATC
Cuts at: 3365 6763 3365
Size: 3398 3371
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 4576 4958 5593
Size: 53 83 186 936 552 2491 382 635
Cuts at: 5593 275
Size: 1451
Fragments arranged by size:
2491 1451 936 635 552 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5202 5600 5874 6021 6432 5202
Size: 398 274 147 411 5539
Fragments arranged by size:
5539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6544
Fragments arranged by size:
6544 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2554 3335 4007 1186
Size: 1368 781 672 3948
Fragments arranged by size:
3948 1368 781 672
BseYI C'CCAG_C
Cuts at: 2392 2529 2542 2670 3156 3963 5972 6468 2392
Size: 137 13 128 486 807 2009 496 2693
Fragments arranged by size:
2693 2009 807 496 486 137 128 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2820 4008 1163
Size: 573 1084 1188 3924
Fragments arranged by size:
3924 1188 1084 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2877 3808 4219 4980 6008 1540
Size: 275 186 876 931 411 761 1028 2301
Fragments arranged by size:
2301 1028 931 876 761 411 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2292 3062 3108 3708 3746 4830 4915 5986
Size: 240 770 46 600 38 1084 85 1071
Cuts at: 5986 2052
Size: 2835
Fragments arranged by size:
2835 1084 1071 770 600 240 85 46
38
BsmI GAATG_Cn'
Cuts at: 2885 4280 4373 2885
Size: 1395 93 5281
Fragments arranged by size:
5281 1395 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2292 2400 2502 2561 2643 2671
Size: 399 262 240 108 102 59 82 28
Cuts at: 2671 2828 3062 3108 3214 3333 3609 3708 3746
Size: 157 234 46 106 119 276 99 38
Cuts at: 3746 3917 4026 4088 4830 4915 5986 1391
Size: 171 109 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 262 240 234
171 157 119 109 108 106 102 99
85 82 62 59 46 38 28
BspEI T'CCGG_A
Cuts at: 6432 6432
Size: 6769
BspHI T'CATG_A
Cuts at: 4658 6388 4658
Size: 1730 5039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2366 3462 3789 4124 844
Size: 1522 1096 327 335 3489
Fragments arranged by size:
3489 1522 1096 335 327
BsrBI CCG'CTC
Cuts at: 1394 1823 3039 4656 1394
Size: 429 1216 1617 3507
Fragments arranged by size:
3507 1617 1216 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5264 5438 65
Size: 1183 4016 174 1396
Fragments arranged by size:
4016 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2437 2872 3645 4004 5413 5600 1700
Size: 110 627 435 773 359 1409 187 2869
Fragments arranged by size:
2869 1409 773 627 435 359 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6769
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6769
BssSI C'ACGA_G
Cuts at: 4829 5841 4829
Size: 1012 5757
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3166 794
Size: 2372 4397
BstBI TT'CG_AA
Cuts at: 5573 5573
Size: 6769
BstEII G'GTnAC_C
Cuts at: 1184 2252 1184
Size: 1068 5701
BstXI CCAn_nnnn'nTGG
Cuts at: 3497 3497
Size: 6769
BstYI r'GATC_y
Cuts at: 1972 2217 3798 4099 4466 4851 4868 6320 6406
Size: 245 1581 301 367 385 17 1452 86
Cuts at: 6406 6418 6764 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1581 1452 385 367 346 301 245
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2269 2751 3614 2269
Size: 482 863 5424
Fragments arranged by size:
5424 863 482
BtgI C'CryG_G
Cuts at: 513 1065 2004 3295 3687 3827 513
Size: 552 939 1291 392 140 3455
Fragments arranged by size:
3455 1291 939 552 392 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6030
Fragments arranged by size:
6030 683 56
BtsI GCAGTG_nn'
Cuts at: 2194 4285 5079 5099 2194
Size: 2091 794 20 3864
Fragments arranged by size:
3864 2091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6769
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3277 3312 529
Size: 35 2713 35 3986
Fragments arranged by size:
3986 2713 35 35
DraI TTT'AAA
Cuts at: 4073 4073
Size: 6769
DraIII CAC_nnn'GTG
Cuts at: 2032 3112 3750 2032
Size: 1080 638 5051
Fragments arranged by size:
5051 1080 638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5776 809
Size: 507 4460 1802
Fragments arranged by size:
4460 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 3624 3973
Size: 54 1425 50 275 18 550 1244 349
Cuts at: 3973 4216 5105 6734 8
Size: 243 889 1629 43
Fragments arranged by size:
1629 1425 1244 889 550 349 275 243
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4216 1537
Size: 275 2404 4090
Fragments arranged by size:
4090 2404 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2448 4699 1353
Size: 1095 2251 3423
Fragments arranged by size:
3423 2251 1095
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3071 3935 5356 5730 5876 387
Size: 1064 138 1482 864 1421 374 146 1280
Fragments arranged by size:
1482 1421 1280 1064 864 374 146 138
EcoICRI GAG'CTC
Cuts at: 2050 2050
Size: 6769
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2343 2603 2682 3029 3550 3926
Size: 393 514 197 260 79 347 521 376
Cuts at: 3926 4040 4832 5429 6216 6484 1239
Size: 114 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 597 521 514 393 376
347 268 260 197 114 79
EcoNI CCTnn'n_nnAGG
Cuts at: 3944 3944
Size: 6769
EcoO109I rG'GnC_Cy
Cuts at: 2104 2396 2397 2526 2557 2558 2649 3561 3605
Size: 292 1 129 31 1 91 912 44
Cuts at: 3605 3606 2104
Size: 1 5267
Fragments arranged by size:
5267 912 292 129 91 44 31 1
1 1
EcoRI G'AATT_C
Cuts at: 4078 4456 4078
Size: 378 6391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 3004 3196 2608
Size: 396 192 6181
Fragments arranged by size:
6181 396 192
FseI GG_CCGG'CC
Cuts at: 3649 3649
Size: 6769
FspI TGC'GCA
Cuts at: 3458 5275 3458
Size: 1817 4952
HaeII r_GCGC'y
Cuts at: 1678 3592 3887 5916 1678
Size: 1914 295 2029 2531
Fragments arranged by size:
2531 2029 1914 295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 2726 2758 5410
Size: 32 135 32 889 32 233 32 2652
Cuts at: 5410 5442 5484 5516 1373
Size: 32 42 32 2626
Fragments arranged by size:
2652 2626 889 233 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4113 4359 669
Size: 988 281 2175 246 3079
Fragments arranged by size:
3079 2175 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3282 748
Size: 2534 4235
HpaI GTT'AAC
Cuts at: 4359 4359
Size: 6769
KpnI G_GTAC'C
Cuts at: 4094 4094
Size: 6769
MfeI C'AATT_G
Cuts at: 4368 4368
Size: 6769
MluI A'CGCG_T
Cuts at: 6645 6645
Size: 6769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 5883 6067 1305
Size: 909 3669 184 2007
Fragments arranged by size:
3669 2007 909 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2382 3626 10
Size: 54 1425 343 550 1244 3153
Fragments arranged by size:
3153 1425 1244 550 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2127 3297 3384 3400 3402 3468 3495
Size: 648 961 1170 87 16 2 66 27
Cuts at: 3495 4727 5086 5245 518
Size: 1232 359 159 2042
Fragments arranged by size:
2042 1232 1170 961 648 359 159 87
66 27 16 2
MspA1I CmG'CkG
Cuts at: 1606 2062 2674 2804 2837 2867 3274 3829 4043
Size: 456 612 130 33 30 407 555 214
Cuts at: 4043 4185 4862 6010 6255 1606
Size: 142 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 612 555 456 407 245
214 142 130 33 30
NaeI GCC'GGC
Cuts at: 2439 3647 2439
Size: 1208 5561
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5278
Fragments arranged by size:
5278 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6769
NgoMIV G'CCGG_C
Cuts at: 2437 3645 2437
Size: 1208 5561
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6769
NotI GC'GGCC_GC
Cuts at: 4216 4216
Size: 6769
NsiI A_TGCA'T
Cuts at: 3229 3229
Size: 6769
NspI r_CATG'y
Cuts at: 1132 1229 2743 2892 3231 3451 4127 5672 1132
Size: 97 1514 149 339 220 676 1545 2229
Fragments arranged by size:
2229 1545 1514 676 339 220 149 97
PasI CC'CwG_GG
Cuts at: 1348 2487 3946 1348
Size: 1139 1459 4171
Fragments arranged by size:
4171 1459 1139
PciI A'CATG_T
Cuts at: 2739 2888 5668 2739
Size: 149 2780 3840
Fragments arranged by size:
3840 2780 149
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6769
PfoI T'CCnGG_A
Cuts at: 3006 4017 3006
Size: 1011 5758
PmeI GTTT'AAAC
Cuts at: 4073 4073
Size: 6769
PmlI CAC'GTG
Cuts at: 3057 3622 3057
Size: 565 6204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4838 4870 6351 6383 4838
Size: 32 1481 32 5224
Fragments arranged by size:
5224 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6769
PsiI TTA'TAA
Cuts at: 4339 4339
Size: 6769
PspOMI G'GGCC_C
Cuts at: 2396 2557 3605 2396
Size: 161 1048 5560
Fragments arranged by size:
5560 1048 161
PspXI vC'TCGA_Gb
Cuts at: 3340 3340
Size: 6769
PstI C_TGCA'G
Cuts at: 830 2046 2147 2679 3273 3360 3416 3891 3901
Size: 1216 101 532 594 87 56 475 10
Cuts at: 3901 4121 830
Size: 220 3478
Fragments arranged by size:
3478 1216 594 532 475 220 101 87
56 10
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6769
PvuII CAG'CTG
Cuts at: 2674 2804 2837 3274 4043 2674
Size: 130 33 437 769 5400
Fragments arranged by size:
5400 769 437 130 33
SacI G_AGCT'C
Cuts at: 2052 2052
Size: 6769
SacII CC_GC'GG
Cuts at: 3830 3830
Size: 6769
SalI G'TCGA_C
Cuts at: 1936 4111 1936
Size: 2175 4594
SbfI CC_TGCA'GG
Cuts at: 4121 4121
Size: 6769
ScaI AGT'ACT
Cuts at: 2078 5017 6729 2078
Size: 2939 1712 2118
Fragments arranged by size:
2939 2118 1712
SfcI C'TryA_G
Cuts at: 826 1045 2042 2143 2675 3269 3356 3412 3887
Size: 219 997 101 532 594 87 56 475
Cuts at: 3887 3897 4117 5252 5933 6124 826
Size: 10 220 1135 681 191 1471
Fragments arranged by size:
1471 1135 997 681 594 532 475 220
219 191 101 87 56 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6769
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6769
SmaI CCC'GGG
Cuts at: 1872 4096 1872
Size: 2224 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2609 3340 4873 5774 6036 820
Size: 197 926 666 731 1533 901 262 1553
Fragments arranged by size:
1553 1533 926 901 731 666 262 197
SnaBI TAC'GTA
Cuts at: 493 4560 493
Size: 4067 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6769
SphI G_CATG'C
Cuts at: 1132 3231 4127 1132
Size: 2099 896 3774
Fragments arranged by size:
3774 2099 896
SspI AAT'ATT
Cuts at: 5 52 2915 4693 5
Size: 47 2863 1778 2081
Fragments arranged by size:
2863 2081 1778 47
StuI AGG'CCT
Cuts at: 1517 2591 1517
Size: 1074 5695
StyI C'CwwG_G
Cuts at: 513 1065 2004 2645 4208 513
Size: 552 939 641 1563 3074
Fragments arranged by size:
3074 1563 939 641 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3443 3560 4796 4813 4966 5151 5596
Size: 1255 1863 117 1236 17 153 185 445
Cuts at: 5596 325
Size: 1498
Fragments arranged by size:
1863 1498 1255 1236 445 185 153 117
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 5015 6727
Size: 274 80 33 51 1443 99 2939 1712
Cuts at: 6727 96
Size: 138
Fragments arranged by size:
2939 1712 1443 274 138 99 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6729 6761 6729
Size: 32 6737
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6769
XbaI T'CTAG_A
Cuts at: 4105 4105
Size: 6769
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2095 2359 2095
Size: 264 6505
XhoI C'TCGA_G
Cuts at: 1943 3340 1943
Size: 1397 5372
XmaI C'CCGG_G
Cuts at: 1870 4094 1870
Size: 2224 4545
XmnI GAAnn'nnTTC
Cuts at: 3479 4898 3479
Size: 1419 5350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4577 276
Size: 53 83 186 936 3043 2468
Fragments arranged by size:
3043 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BsiWI BsmBI BstZ17I EcoRV
FspAI KasI NarI NruI PacI PpuMI PsrI RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI