Restriction map for
pFN21AB6261
(Circular) (Six-base) MAPSORT of: pFN21AB6261.seq Check: 7704 from: 1 to: 6763
pFN21AB6261 6763 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4573 278
Size: 53 83 186 936 3037 2468
Fragments arranged by size:
3037 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4106 1937
Size: 2169 4594
Acc65I G'GTAC_C
Cuts at: 3938 4084 3938
Size: 146 6617
AclI AA'CG_TT
Cuts at: 1563 3206 4890 5263 1563
Size: 1643 1684 373 3063
Fragments arranged by size:
3063 1684 1643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2748 3111 3134 4826 6210 6478 1632
Size: 1116 363 23 1692 1384 268 1917
Fragments arranged by size:
1917 1692 1384 1116 363 268 23
AflII C'TTAA_G
Cuts at: 820 1017 3928 820
Size: 197 2911 3655
Fragments arranged by size:
3655 2911 197
AflIII A'CryG_T
Cuts at: 1203 5662 6639 1203
Size: 4459 977 1327
Fragments arranged by size:
4459 1327 977
AgeI A'CCGG_T
Cuts at: 5594 5594
Size: 6763
AhdI GACnn_n'nnGTC
Cuts at: 2458 3673 5492 2458
Size: 1215 1819 3729
Fragments arranged by size:
3729 1819 1215
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2085 1184
Size: 901 5862
AlwNI CAG_nnn'CTG
Cuts at: 2128 2978 4506 6078 2128
Size: 850 1528 1572 2813
Fragments arranged by size:
2813 1572 1528 850
ApaLI G'TGCA_C
Cuts at: 4820 5976 4820
Size: 1156 5607
ApoI r'AATT_y
Cuts at: 1493 2690 3782 3862 4072 4298 4450 6559 1493
Size: 1197 1092 80 210 226 152 2109 1697
Fragments arranged by size:
2109 1697 1197 1092 226 210 152 80
AseI AT'TA_AT
Cuts at: 160 1032 5317 160
Size: 872 4285 1606
Fragments arranged by size:
4285 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
AvaI C'yCGr_G
Cuts at: 1870 1943 4088 1870
Size: 73 2145 4545
Fragments arranged by size:
4545 2145 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2557 2590 2557
Size: 33 6730
BamHI G'GATC_C
Cuts at: 4093 4460 4093
Size: 367 6396
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 3938 4084 5539 618
Size: 325 217 627 222 1929 146 1455 1842
Fragments arranged by size:
1929 1842 1455 627 325 222 217 146
BanII G_rGCy'C
Cuts at: 1391 2253 2312 3298 3387 4082 1391
Size: 862 59 986 89 695 4072
Fragments arranged by size:
4072 986 862 695 89 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2868 2883 928
Size: 971 969 15 4808
Fragments arranged by size:
4808 971 969 15
BbvCI CC'TCA_GC
Cuts at: 2057 2738 2057
Size: 681 6082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2188 2222 4954 4988 1903
Size: 34 251 34 2732 34 3678
Fragments arranged by size:
3678 2732 251 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4655 5865 4655
Size: 1210 5553
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6763
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5374 136
Size: 107 122 71 631 605 3702 1525
Fragments arranged by size:
3702 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6758 6758
Size: 6763
BlpI GC'TnA_GC
Cuts at: 4180 4180
Size: 6763
Bme1580I G_kGCm'C
Cuts at: 1790 2012 4824 5980 1790
Size: 222 2812 1156 2573
Fragments arranged by size:
2812 2573 1156 222
BmgBI CAC'GTC
Cuts at: 1318 2455 1318
Size: 1137 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2876 3689 3928
Size: 416 53 83 399 24 1459 813 239
Cuts at: 3928 4494 5452 442
Size: 566 958 1753
Fragments arranged by size:
1753 1459 958 813 566 416 399 239
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2021 2108 2114 3597 5423 1239
Size: 782 87 6 1483 1826 2579
Fragments arranged by size:
2579 1826 1483 782 87 6
Bpu10I CC'TnA_GC
Cuts at: 2057 2738 2057
Size: 681 6082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3209 3488 4888 5753 6051 3209
Size: 279 1400 865 298 3921
Fragments arranged by size:
3921 1400 865 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2759 5426 882
Size: 617 1260 2667 2219
Fragments arranged by size:
2667 2219 1260 617
BsaAI yAC'GTr
Cuts at: 493 1204 4554 493
Size: 711 3350 2702
Fragments arranged by size:
3350 2702 711
BsaBI GATnn'nnATC
Cuts at: 6757 6757
Size: 6763
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4570 4952 5587 275
Size: 53 83 186 936 3037 382 635 1451
Fragments arranged by size:
3037 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5196 5594 5868 6015 6426 5196
Size: 398 274 147 411 5533
Fragments arranged by size:
5533 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 1178
Size: 30 165 30 678 30 5830
Fragments arranged by size:
5830 678 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 5883
BseYI C'CCAG_C
Cuts at: 2175 5966 6462 2175
Size: 3791 496 2476
Fragments arranged by size:
3791 2476 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6190
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2589 4213 4974 6002 1540
Size: 275 186 588 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 588 275 186
BsiHKAI G_wGCw'C
Cuts at: 2204 3264 3387 4824 4909 5980 2204
Size: 1060 123 1437 85 1071 2987
Fragments arranged by size:
2987 1437 1071 1060 123 85
BsmI GAATG_Cn'
Cuts at: 3475 4274 4367 3475
Size: 799 93 5871
Fragments arranged by size:
5871 799 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2445 2445
Size: 6763
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2204 2253 2312 3264 3298 3387
Size: 399 222 192 49 59 952 34 89
Cuts at: 3387 4082 4824 4909 5980 1391
Size: 695 742 85 1071 2174
Fragments arranged by size:
2174 1071 952 742 695 399 222 192
89 85 59 49 34
BspEI T'CCGG_A
Cuts at: 6426 6426
Size: 6763
BspHI T'CATG_A
Cuts at: 4652 6382 4652
Size: 1730 5033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 4118 844
Size: 2149 1125 3489
Fragments arranged by size:
3489 2149 1125
BsrBI CCG'CTC
Cuts at: 1394 1823 2154 3270 4650 1394
Size: 429 331 1116 1380 3507
Fragments arranged by size:
3507 1380 1116 429 331
BsrDI GCAATG_nn'
Cuts at: 65 1248 3219 5258 5432 65
Size: 1183 1971 2039 174 1396
Fragments arranged by size:
2039 1971 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5407 5594 1700
Size: 110 3597 187 2869
Fragments arranged by size:
3597 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3321 96
Size: 3225 3538
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6763
BssSI C'ACGA_G
Cuts at: 2856 4823 5835 2856
Size: 1967 1012 3784
Fragments arranged by size:
3784 1967 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3286 794
Size: 2492 4271
BstBI TT'CG_AA
Cuts at: 5567 5567
Size: 6763
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6763
BstYI r'GATC_y
Cuts at: 1972 2272 2323 2382 2733 3733 4093 4460 4845
Size: 300 51 59 351 1000 360 367 385
Cuts at: 4845 4862 6314 6400 6412 6758 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1000 385 367 360 351 346
300 86 59 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 4008 4008
Size: 6763
BtgI C'CryG_G
Cuts at: 513 1065 2004 2526 513
Size: 552 939 522 4750
Fragments arranged by size:
4750 939 552 522
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6024
Fragments arranged by size:
6024 683 56
BtsI GCAGTG_nn'
Cuts at: 4279 5073 5093 4279
Size: 794 20 5949
Fragments arranged by size:
5949 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6763
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6728
DraI TTT'AAA
Cuts at: 2697 3013 4067 2697
Size: 316 1054 5393
Fragments arranged by size:
5393 1054 316
DraIII CAC_nnn'GTG
Cuts at: 2873 3241 2873
Size: 368 6395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5770 809
Size: 507 4454 1802
Fragments arranged by size:
4454 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4210 5099 6728
Size: 54 1425 50 275 18 2380 889 1629
Cuts at: 6728 8
Size: 43
Fragments arranged by size:
2380 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4210 1537
Size: 275 2398 4090
Fragments arranged by size:
4090 2398 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2724 2757 4693 1353
Size: 1371 33 1936 3423
Fragments arranged by size:
3423 1936 1371 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2233 5350 5724 5870 387
Size: 1064 138 644 3117 374 146 1280
Fragments arranged by size:
3117 1280 1064 644 374 146 138
EcoICRI GAG'CTC
Cuts at: 3385 3385
Size: 6763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2108 2114 2748 3111 3134 3597
Size: 393 389 87 6 634 363 23 463
Cuts at: 3597 4826 5423 6210 6478 1239
Size: 1229 597 787 268 1524
Fragments arranged by size:
1524 1229 787 634 597 463 393 389
363 268 87 23 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3723 3723
Size: 6763
EcoO109I rG'GnC_Cy
Cuts at: 3499 3499
Size: 6763
EcoRI G'AATT_C
Cuts at: 4072 4450 4072
Size: 378 6385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2360 2360
Size: 6763
FspI TGC'GCA
Cuts at: 5269 5269
Size: 6763
HaeII r_GCGC'y
Cuts at: 1678 5910 1678
Size: 4232 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 2990 3022 3659
Size: 32 135 32 872 32 514 32 637
Cuts at: 3659 3691 3830 3862 4025 4057 5404 5436 5478
Size: 32 139 32 163 32 1347 32 42
Cuts at: 5478 5510 1373
Size: 32 2626
Fragments arranged by size:
2626 1347 872 637 514 163 139 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4107 4353 669
Size: 988 281 2169 246 3079
Fragments arranged by size:
3079 2169 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2744 3366 3459 748
Size: 1996 622 93 4052
Fragments arranged by size:
4052 1996 622 93
HpaI GTT'AAC
Cuts at: 4353 4353
Size: 6763
KpnI G_GTAC'C
Cuts at: 3942 4088 3942
Size: 146 6617
MfeI C'AATT_G
Cuts at: 4362 4362
Size: 6763
MluI A'CGCG_T
Cuts at: 6639 6639
Size: 6763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 2188 2732 3133 5877 6061 1305
Size: 774 109 544 401 2744 184 2007
Fragments arranged by size:
2744 2007 774 544 401 184 109
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4941
Fragments arranged by size:
4941 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4721 5080 5239 518
Size: 648 3555 359 159 2042
Fragments arranged by size:
3555 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 4179 4856 6004 6249 1606
Size: 459 2114 677 1148 245 2120
Fragments arranged by size:
2120 2114 1148 677 459 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2526 513
Size: 552 939 522 4750
Fragments arranged by size:
4750 939 552 522
NdeI CA'TA_TG
Cuts at: 387 3055 387
Size: 2668 4095
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6763
NotI GC'GGCC_GC
Cuts at: 4210 4210
Size: 6763
NspI r_CATG'y
Cuts at: 1132 1229 3450 4121 5666 1132
Size: 97 2221 671 1545 2229
Fragments arranged by size:
2229 2221 1545 671 97
PacI TTA_AT'TAA
Cuts at: 3822 3822
Size: 6763
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6763
PciI A'CATG_T
Cuts at: 5662 5662
Size: 6763
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6763
PfoI T'CCnGG_A
Cuts at: 2037 2268 2385 2533 2037
Size: 231 117 148 6267
Fragments arranged by size:
6267 231 148 117
PmeI GTTT'AAAC
Cuts at: 4067 4067
Size: 6763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4832 4864 6345 6377 4832
Size: 32 1481 32 5218
Fragments arranged by size:
5218 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3499 3499
Size: 6763
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6763
PsiI TTA'TAA
Cuts at: 2330 4333 2330
Size: 2003 4760
PstI C_TGCA'G
Cuts at: 830 2133 2974 4115 830
Size: 1303 841 1141 3478
Fragments arranged by size:
3478 1303 1141 841
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6763
SacI G_AGCT'C
Cuts at: 3387 3387
Size: 6763
SalI G'TCGA_C
Cuts at: 1936 4105 1936
Size: 2169 4594
SbfI CC_TGCA'GG
Cuts at: 4115 4115
Size: 6763
ScaI AGT'ACT
Cuts at: 5011 6723 5011
Size: 1712 5051
SfcI C'TryA_G
Cuts at: 826 1045 2129 2970 4111 5246 5927 6118 826
Size: 219 1084 841 1141 1135 681 191 1471
Fragments arranged by size:
1471 1141 1135 1084 841 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6763
SmaI CCC'GGG
Cuts at: 1872 4090 1872
Size: 2218 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 3503 3928 4867 5768 6030
Size: 197 926 1281 279 425 939 901 262
Cuts at: 6030 820
Size: 1553
Fragments arranged by size:
1553 1281 939 926 901 425 279 262
197
SnaBI TAC'GTA
Cuts at: 493 4554 493
Size: 4061 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6763
SphI G_CATG'C
Cuts at: 1132 4121 1132
Size: 2989 3774
SspI AAT'ATT
Cuts at: 5 52 3347 4687 5
Size: 47 3295 1340 2081
Fragments arranged by size:
3295 2081 1340 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6763
StyI C'CwwG_G
Cuts at: 513 1065 2004 2319 2526 3145 4202 513
Size: 552 939 315 207 619 1057 3074
Fragments arranged by size:
3074 1057 939 619 552 315 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 3991 4790 4807 4960 5145 5590
Size: 1255 609 1802 799 17 153 185 445
Cuts at: 5590 325
Size: 1498
Fragments arranged by size:
1802 1498 1255 799 609 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3321 5009 6721
Size: 274 80 33 51 1443 1344 1688 1712
Cuts at: 6721 96
Size: 138
Fragments arranged by size:
1712 1688 1443 1344 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3589 3621 3669 3701 6723 6755 3589
Size: 32 48 32 3022 32 3597
Fragments arranged by size:
3597 3022 48 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2882 1316
Size: 1566 5197
XbaI T'CTAG_A
Cuts at: 4099 4099
Size: 6763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2777 2777
Size: 6763
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6763
XmaI C'CCGG_G
Cuts at: 1870 4088 1870
Size: 2218 4545
XmnI GAAnn'nnTTC
Cuts at: 4892 4892
Size: 6763
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4571 276
Size: 53 83 186 936 3037 2468
Fragments arranged by size:
3037 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI