Restriction map for
pFN21AB6259
(Circular) (Six-base) MAPSORT of: pFN21AB6259.seq Check: 9617 from: 1 to: 6997
pFN21AB6259 6997 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2897 2897
Size: 6997
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4807 278
Size: 53 83 186 936 3271 2468
Fragments arranged by size:
3271 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2298 4340 1937
Size: 361 2042 4594
Fragments arranged by size:
4594 2042 361
Acc65I G'GTAC_C
Cuts at: 4318 4318
Size: 6997
AclI AA'CG_TT
Cuts at: 1563 5124 5497 1563
Size: 3561 373 3063
Fragments arranged by size:
3561 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3975 5060 6444 6712 1632
Size: 2343 1085 1384 268 1917
Fragments arranged by size:
2343 1917 1384 1085 268
AfeI AGC'GCT
Cuts at: 2846 2846
Size: 6997
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6800
AflIII A'CryG_T
Cuts at: 1203 5896 6873 1203
Size: 4693 977 1327
Fragments arranged by size:
4693 1327 977
AgeI A'CCGG_T
Cuts at: 4089 5828 4089
Size: 1739 5258
AhdI GACnn_n'nnGTC
Cuts at: 5726 5726
Size: 6997
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6997
AlwNI CAG_nnn'CTG
Cuts at: 4740 6312 4740
Size: 1572 5425
ApaLI G'TGCA_C
Cuts at: 5054 6210 5054
Size: 1156 5841
ApoI r'AATT_y
Cuts at: 1493 2684 2882 3612 3702 4022 4097 4292 4306
Size: 1191 198 730 90 320 75 195 14
Cuts at: 4306 4532 4684 6793 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1191 730 320 226 198 195
152 90 75 14
AseI AT'TA_AT
Cuts at: 160 1032 5551 160
Size: 872 4519 1606
Fragments arranged by size:
4519 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 4322 1870
Size: 73 1512 867 4545
Fragments arranged by size:
4545 1512 867 73
BamHI G'GATC_C
Cuts at: 4327 4694 4327
Size: 367 6630
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3254 3301 4276 4318 5773
Size: 325 217 627 1467 47 975 42 1455
Cuts at: 5773 618
Size: 1842
Fragments arranged by size:
1842 1467 1455 975 627 325 217 47
42
BanII G_rGCy'C
Cuts at: 1391 4316 1391
Size: 2925 4072
BbeI G_GCGC'C
Cuts at: 3305 3305
Size: 6997
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 2777 928
Size: 971 199 679 5148
Fragments arranged by size:
5148 971 679 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5188 5222 1903
Size: 34 3251 34 3678
Fragments arranged by size:
3678 3251 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2830 2923 4889 6099 2830
Size: 93 1966 1210 3728
Fragments arranged by size:
3728 1966 1210 93
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6997
BfrBI ATG'CAT
Cuts at: 2327 2327
Size: 6997
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5608 136
Size: 107 122 71 631 605 3936 1525
Fragments arranged by size:
3936 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6992 6992
Size: 6997
BlpI GC'TnA_GC
Cuts at: 4414 4414
Size: 6997
Bme1580I G_kGCm'C
Cuts at: 1790 2871 5058 6214 1790
Size: 1081 2187 1156 2573
Fragments arranged by size:
2573 2187 1156 1081
BmgBI CAC'GTC
Cuts at: 1318 3001 1318
Size: 1683 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2303 3152 4197
Size: 416 53 83 399 24 886 849 1045
Cuts at: 4197 4728 5686 442
Size: 531 958 1753
Fragments arranged by size:
1753 1045 958 886 849 531 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3181 5657 1239
Size: 1942 2476 2579
Fragments arranged by size:
2579 2476 1942
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5122 5987 6285 5122
Size: 865 298 5834
Fragments arranged by size:
5834 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5660 882
Size: 617 4161 2219
Fragments arranged by size:
4161 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4259 4788 493
Size: 711 3055 529 2702
Fragments arranged by size:
3055 2702 711 529
BsaBI GATnn'nnATC
Cuts at: 3117 3625 6991 3117
Size: 508 3366 3123
Fragments arranged by size:
3366 3123 508
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3302 4804 5186 5821
Size: 53 83 186 936 1769 1502 382 635
Cuts at: 5821 275
Size: 1451
Fragments arranged by size:
1769 1502 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4089 5430 5828 6102 6249 6660 4089
Size: 1341 398 274 147 411 4426
Fragments arranged by size:
4426 1341 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6772
Fragments arranged by size:
6772 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6997
BseYI C'CCAG_C
Cuts at: 2338 3179 3242 6200 6696 2338
Size: 841 63 2958 496 2639
Fragments arranged by size:
2958 2639 841 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6424
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4447 5208 6236 1540
Size: 275 186 2446 761 1028 2301
Fragments arranged by size:
2446 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3221 5058 5143 6214 3221
Size: 1837 85 1071 4004
Fragments arranged by size:
4004 1837 1071 85
BsmI GAATG_Cn'
Cuts at: 3360 4508 4601 3360
Size: 1148 93 5756
Fragments arranged by size:
5756 1148 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2871 3221 4316 5058 5143 6214 1391
Size: 399 1081 350 1095 742 85 1071 2174
Fragments arranged by size:
2174 1095 1081 1071 742 399 350 85
BspEI T'CCGG_A
Cuts at: 6660 6660
Size: 6997
BspHI T'CATG_A
Cuts at: 3423 4886 6616 3423
Size: 1463 1730 3804
Fragments arranged by size:
3804 1730 1463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2897 3885 4352 844
Size: 2053 988 467 3489
Fragments arranged by size:
3489 2053 988 467
BsrBI CCG'CTC
Cuts at: 1394 1823 4884 1394
Size: 429 3061 3507
Fragments arranged by size:
3507 3061 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 3051 5492 5666 65
Size: 1183 1219 584 2441 174 1396
Fragments arranged by size:
2441 1396 1219 1183 584 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 4089 5641 5828 1700
Size: 110 395 1884 1552 187 2869
Fragments arranged by size:
2869 1884 1552 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6997
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6997
BssSI C'ACGA_G
Cuts at: 5057 6069 5057
Size: 1012 5985
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3050 3760 794
Size: 2256 710 4031
Fragments arranged by size:
4031 2256 710
BstBI TT'CG_AA
Cuts at: 3700 5801 3700
Size: 2101 4896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6997
BstXI CCAn_nnnn'nTGG
Cuts at: 3448 3448
Size: 6997
BstYI r'GATC_y
Cuts at: 1972 4327 4694 5079 5096 6548 6634 6646 6992
Size: 2355 367 385 17 1452 86 12 346
Cuts at: 6992 1972
Size: 1977
Fragments arranged by size:
2355 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2382 513
Size: 552 1317 5128
Fragments arranged by size:
5128 1317 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3025 487
Size: 683 56 1799 4459
Fragments arranged by size:
4459 1799 683 56
BtsI GCAGTG_nn'
Cuts at: 3066 3975 4513 5307 5327 3066
Size: 909 538 794 20 4736
Fragments arranged by size:
4736 909 794 538 20
ClaI AT'CG_AT
Cuts at: 1554 3675 4010 1554
Size: 2121 335 4541
Fragments arranged by size:
4541 2121 335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6962
DraI TTT'AAA
Cuts at: 2932 2944 4301 2932
Size: 12 1357 5628
Fragments arranged by size:
5628 1357 12
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2707 6004 809
Size: 507 1391 3297 1802
Fragments arranged by size:
3297 1802 1391 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2262 4444
Size: 54 1425 50 275 18 207 225 2182
Cuts at: 4444 5333 6962 8
Size: 889 1629 43
Fragments arranged by size:
2182 1629 1425 889 275 225 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4444 1537
Size: 275 2632 4090
Fragments arranged by size:
4090 2632 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3816 4927 1353
Size: 2463 1111 3423
Fragments arranged by size:
3423 2463 1111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3679 5584 5958 6104 387
Size: 1064 138 2090 1905 374 146 1280
Fragments arranged by size:
2090 1905 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3181 3975 5060 5657 6444 6712 1239
Size: 393 1549 794 1085 597 787 268 1524
Fragments arranged by size:
1549 1524 1085 794 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3510 3925 4000 3510
Size: 415 75 6507
Fragments arranged by size:
6507 415 75
EcoRI G'AATT_C
Cuts at: 2882 3702 4306 4684 2882
Size: 820 604 378 5195
Fragments arranged by size:
5195 820 604 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2876 3775 2876
Size: 899 6098
FspI TGC'GCA
Cuts at: 5503 5503
Size: 6997
HaeII r_GCGC'y
Cuts at: 1678 2848 3305 6144 1678
Size: 1170 457 2839 2531
Fragments arranged by size:
2839 2531 1170 457
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5638 5670 5712 5744 1373
Size: 32 135 32 4066 32 42 32 2626
Fragments arranged by size:
4066 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2299 2700 3799 4341 4587 669
Size: 988 281 361 401 1099 542 246 3079
Fragments arranged by size:
3079 1099 988 542 401 361 281 246
HindIII A'AGCT_T
Cuts at: 748 3723 4082 748
Size: 2975 359 3663
Fragments arranged by size:
3663 2975 359
HpaI GTT'AAC
Cuts at: 4587 4587
Size: 6997
KasI G'GCGC_C
Cuts at: 3301 3301
Size: 6997
KpnI G_GTAC'C
Cuts at: 4322 4322
Size: 6997
MfeI C'AATT_G
Cuts at: 4596 4596
Size: 6997
MluI A'CGCG_T
Cuts at: 6873 6873
Size: 6997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4036 6111 6295 1305
Size: 2731 2075 184 2007
Fragments arranged by size:
2731 2075 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 10
Size: 54 1425 343 207 4968
Fragments arranged by size:
4968 1425 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 3044 3263 4955 5314 5473 518
Size: 648 1215 663 219 1692 359 159 2042
Fragments arranged by size:
2042 1692 1215 663 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 4413 5090 6238 6483 1606
Size: 2807 677 1148 245 2120
Fragments arranged by size:
2807 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3302 3302
Size: 6997
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6445
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6997
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6997
NotI GC'GGCC_GC
Cuts at: 4444 4444
Size: 6997
NsiI A_TGCA'T
Cuts at: 2329 2329
Size: 6997
NspI r_CATG'y
Cuts at: 1132 1229 4355 5900 1132
Size: 97 3126 1545 2229
Fragments arranged by size:
3126 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6997
PciI A'CATG_T
Cuts at: 5896 5896
Size: 6997
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2315 3113 1108
Size: 1207 798 4992
Fragments arranged by size:
4992 1207 798
PfoI T'CCnGG_A
Cuts at: 2971 2971
Size: 6997
PmeI GTTT'AAAC
Cuts at: 4301 4301
Size: 6997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5066 5098 6579 6611 5066
Size: 32 1481 32 5452
Fragments arranged by size:
5452 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3510 3925 3510
Size: 415 6582
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6997
PsiI TTA'TAA
Cuts at: 4567 4567
Size: 6997
PstI C_TGCA'G
Cuts at: 830 3750 4349 830
Size: 2920 599 3478
Fragments arranged by size:
3478 2920 599
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6997
SalI G'TCGA_C
Cuts at: 1936 2297 4339 1936
Size: 361 2042 4594
Fragments arranged by size:
4594 2042 361
SapI GCTCTTCn'nnn_
Cuts at: 3816 3816
Size: 6997
SbfI CC_TGCA'GG
Cuts at: 4349 4349
Size: 6997
ScaI AGT'ACT
Cuts at: 2742 3187 3849 4186 5245 6957 2742
Size: 445 662 337 1059 1712 2782
Fragments arranged by size:
2782 1712 1059 662 445 337
SexAI A'CCwGG_T
Cuts at: 2787 2787
Size: 6997
SfcI C'TryA_G
Cuts at: 826 1045 3746 4345 5480 6161 6352 826
Size: 219 2701 599 1135 681 191 1471
Fragments arranged by size:
2701 1471 1135 681 599 219 191
SfoI GGC'GCC
Cuts at: 3303 3303
Size: 6997
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6997
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6997
SmaI CCC'GGG
Cuts at: 1872 4324 1872
Size: 2452 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5101 6002 6264 820
Size: 197 926 3158 901 262 1553
Fragments arranged by size:
3158 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4259 4788 493
Size: 3766 529 2702
Fragments arranged by size:
3766 2702 529
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6997
SphI G_CATG'C
Cuts at: 1132 4355 1132
Size: 3223 3774
SspI AAT'ATT
Cuts at: 5 52 2779 3544 4128 4921 5
Size: 47 2727 765 584 793 2081
Fragments arranged by size:
2727 2081 793 765 584 47
StuI AGG'CCT
Cuts at: 1517 3465 1517
Size: 1948 5049
StyI C'CwwG_G
Cuts at: 513 1065 3135 4436 513
Size: 552 2070 1301 3074
Fragments arranged by size:
3074 2070 1301 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5024 5041 5194 5379 5824 325
Size: 1255 3444 17 153 185 445 1498
Fragments arranged by size:
3444 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2740 3185 3791
Size: 274 80 33 51 1443 763 445 606
Cuts at: 3791 3847 4184 5243 6955 96
Size: 56 337 1059 1712 138
Fragments arranged by size:
1712 1443 1059 763 606 445 337 274
138 80 56 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2592 2624 6957 6989 2592
Size: 32 4333 32 2600
Fragments arranged by size:
4333 2600 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6997
XbaI T'CTAG_A
Cuts at: 4333 4333
Size: 6997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2579
Size: 6997
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6997
XmaI C'CCGG_G
Cuts at: 1870 4322 1870
Size: 2452 4545
XmnI GAAnn'nnTTC
Cuts at: 3614 5126 3614
Size: 1512 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4805 276
Size: 53 83 186 936 3271 2468
Fragments arranged by size:
3271 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SrfI SwaI