Restriction map for
pFN21AB6256
(Circular) (Six-base) MAPSORT of: pFN21AB6256.seq Check: 2272 from: 1 to: 6754
pFN21AB6256 6754 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3236 4564 278
Size: 53 83 186 936 1700 1328 2468
Fragments arranged by size:
2468 1700 1328 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4097 1937
Size: 2160 4594
Acc65I G'GTAC_C
Cuts at: 4075 4075
Size: 6754
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2076 2948 3336 3986 4817 6201 6469 1632
Size: 444 872 388 650 831 1384 268 1917
Fragments arranged by size:
1917 1384 872 831 650 444 388 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 5653 6630 1203
Size: 4450 977 1327
Fragments arranged by size:
4450 1327 977
AgeI A'CCGG_T
Cuts at: 5585 5585
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 5483 5483
Size: 6754
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3284 1184
Size: 2100 4654
AlwNI CAG_nnn'CTG
Cuts at: 2447 2638 2903 3157 3766 4497 6069 2447
Size: 191 265 254 609 731 1572 3132
Fragments arranged by size:
3132 1572 731 609 265 254 191
ApaI G_GGCC'C
Cuts at: 3524 3524
Size: 6754
ApaLI G'TGCA_C
Cuts at: 4811 5967 4811
Size: 1156 5598
ApoI r'AATT_y
Cuts at: 1493 3437 4063 4289 4441 6550 1493
Size: 1944 626 226 152 2109 1697
Fragments arranged by size:
2109 1944 1697 626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 4079 1870
Size: 73 2136 4545
Fragments arranged by size:
4545 2136 73
BamHI G'GATC_C
Cuts at: 4084 4451 4084
Size: 367 6387
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2611 2713 3462 4075 5530
Size: 325 217 627 824 102 749 613 1455
Cuts at: 5530 618
Size: 1842
Fragments arranged by size:
1842 1455 824 749 627 613 325 217
102
BanII G_rGCy'C
Cuts at: 1391 2115 2789 2921 3524 4026 4073 1391
Size: 724 674 132 603 502 47 4072
Fragments arranged by size:
4072 724 674 603 502 132 47
BbeI G_GCGC'C
Cuts at: 2615 2717 3466 2615
Size: 102 749 5903
Fragments arranged by size:
5903 749 102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2954 928
Size: 971 1055 4728
Fragments arranged by size:
4728 1055 971
BbvCI CC'TCA_GC
Cuts at: 2718 3729 3735 2718
Size: 1011 6 5737
Fragments arranged by size:
5737 1011 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2149 2183 4945 4979 1903
Size: 34 212 34 2762 34 3678
Fragments arranged by size:
3678 2762 212 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3660 4646 5856 3660
Size: 986 1210 4558
Fragments arranged by size:
4558 1210 986
BclI T'GATC_A
Cuts at: 1548 3165 1548
Size: 1617 5137
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2609 5365 136
Size: 107 122 71 631 605 937 2756 1525
Fragments arranged by size:
2756 1525 937 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6749 6749
Size: 6754
BlpI GC'TnA_GC
Cuts at: 4171 4171
Size: 6754
Bme1580I G_kGCm'C
Cuts at: 1790 3524 3694 4815 5971 1790
Size: 1734 170 1121 1156 2573
Fragments arranged by size:
2573 1734 1156 1121 170
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6754
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4485 5443 442
Size: 416 53 83 399 24 3068 958 1753
Fragments arranged by size:
3068 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3615 1056
Size: 2559 4195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2510 2516 2522 2773 2802 2941 3443
Size: 1262 9 6 6 251 29 139 502
Cuts at: 3443 5414 1239
Size: 1971 2579
Fragments arranged by size:
2579 1971 1262 502 251 139 29 9
6 6
Bpu10I CC'TnA_GC
Cuts at: 2718 3729 3735 2718
Size: 1011 6 5737
Fragments arranged by size:
5737 1011 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3734 3857 4879 5744 6042 3734
Size: 123 1022 865 298 4446
Fragments arranged by size:
4446 1022 865 298 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5417 882
Size: 617 3918 2219
Fragments arranged by size:
3918 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4545 493
Size: 711 3341 2702
Fragments arranged by size:
3341 2702 711
BsaBI GATnn'nnATC
Cuts at: 6748 6748
Size: 6754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2612 2714 3233 3463
Size: 53 83 186 936 1079 102 519 230
Cuts at: 3463 4561 4943 5578 275
Size: 1098 382 635 1451
Fragments arranged by size:
1451 1098 1079 936 635 519 382 230
186 102 83 53
BsaWI w'CCGG_w
Cuts at: 5187 5585 5859 6006 6417 5187
Size: 398 274 147 411 5524
Fragments arranged by size:
5524 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 3209 3239 1178
Size: 30 165 30 1329 30 447 30 4693
Fragments arranged by size:
4693 1329 447 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3095 3325 3334 3440 1186
Size: 1909 230 9 106 4500
Fragments arranged by size:
4500 1909 230 106 9
BseYI C'CCAG_C
Cuts at: 2156 2185 2323 2511 2625 2655 2724 2778 3099
Size: 29 138 188 114 30 69 54 321
Cuts at: 3099 5957 6453 2156
Size: 2858 496 2457
Fragments arranged by size:
2858 2457 496 321 188 138 114 69
54 30 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3104 3254 1163
Size: 573 1368 150 4663
Fragments arranged by size:
4663 1368 573 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4204 4965 5993 1540
Size: 275 186 2203 761 1028 2301
Fragments arranged by size:
2301 2203 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2789 2921 4815 4900 5971 2789
Size: 132 1894 85 1071 3572
Fragments arranged by size:
3572 1894 1071 132 85
BsmI GAATG_Cn'
Cuts at: 2062 4265 4358 2062
Size: 2203 93 4458
Fragments arranged by size:
4458 2203 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2789 2921 3524 3694 4026 4073
Size: 399 325 674 132 603 170 332 47
Cuts at: 4073 4815 4900 5971 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 603 399 332 325
170 132 85 47
BspEI T'CCGG_A
Cuts at: 6417 6417
Size: 6754
BspHI T'CATG_A
Cuts at: 4643 6373 4643
Size: 1730 5024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3743 4109 844
Size: 2899 366 3489
Fragments arranged by size:
3489 2899 366
BsrBI CCG'CTC
Cuts at: 1394 1823 2842 3718 4641 1394
Size: 429 1019 876 923 3507
Fragments arranged by size:
3507 1019 923 876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2953 5249 5423 65
Size: 1183 1705 2296 174 1396
Fragments arranged by size:
2296 1705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2300 3706 4000 5398 5585 1700
Size: 110 490 1406 294 1398 187 2869
Fragments arranged by size:
2869 1406 1398 490 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6754
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 3995 4814 5826 3995
Size: 819 1012 4923
Fragments arranged by size:
4923 1012 819
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2447 2638 2903 794
Size: 1653 191 265 4645
Fragments arranged by size:
4645 1653 265 191
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6754
BstYI r'GATC_y
Cuts at: 1972 3153 3429 3840 4084 4451 4836 4853 6305
Size: 1181 276 411 244 367 385 17 1452
Cuts at: 6305 6391 6403 6749 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1181 411 385 367 346 276
244 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4005 513
Size: 552 2940 3262
Fragments arranged by size:
3262 2940 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3115 487
Size: 683 56 1889 4126
Fragments arranged by size:
4126 1889 683 56
BtsI GCAGTG_nn'
Cuts at: 2399 2474 4270 5064 5084 2399
Size: 75 1796 794 20 4069
Fragments arranged by size:
4069 1796 794 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3124 3159 529
Size: 35 2560 35 4124
Fragments arranged by size:
4124 2560 35 35
DraI TTT'AAA
Cuts at: 3454 4058 3454
Size: 604 6150
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5761 809
Size: 507 4445 1802
Fragments arranged by size:
4445 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 3205 3540
Size: 54 1425 50 275 18 468 907 335
Cuts at: 3540 4002 4201 5090 6719 8
Size: 462 199 889 1629 43
Fragments arranged by size:
1629 1425 907 889 468 462 335 275
199 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 3331 3352 4684 1353
Size: 818 1160 21 1332 3423
Fragments arranged by size:
3423 1332 1160 818 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5341 5715 5861 387
Size: 1064 138 3752 374 146 1280
Fragments arranged by size:
3752 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2787 2919 2787
Size: 132 6622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2501 2510 2516 2522 2773 2802
Size: 393 444 425 9 6 6 251 29
Cuts at: 2802 2941 2948 3336 3443 3986 4817 5414 6201
Size: 139 7 388 107 543 831 597 787
Cuts at: 6201 6469 1239
Size: 268 1524
Fragments arranged by size:
1524 831 787 597 543 444 425 393
388 268 251 139 107 29 9 7
6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3036 3584 3036
Size: 548 6206
EcoO109I rG'GnC_Cy
Cuts at: 2318 3520 3521 3832 2318
Size: 1202 1 311 5240
Fragments arranged by size:
5240 1202 311 1
EcoRI G'AATT_C
Cuts at: 4063 4441 4063
Size: 378 6376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3281 3281
Size: 6754
FspI TGC'GCA
Cuts at: 5260 5260
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 2615 2717 3466 5901 1678
Size: 937 102 749 2435 2531
Fragments arranged by size:
2531 2435 937 749 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2379 2411 2994 3019 3026
Size: 32 135 32 807 32 583 25 7
Cuts at: 3026 3051 3472 3489 3504 3521 3543 3575 5395
Size: 25 421 17 15 17 22 32 1820
Cuts at: 5395 5427 5469 5501 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1820 807 583 421 135 42 32
32 32 32 32 32 25 25 22
17 17 15 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2580 2981 4098 4344 669
Size: 988 281 642 401 1117 246 3079
Fragments arranged by size:
3079 1117 988 642 401 281 246
HindIII A'AGCT_T
Cuts at: 748 3447 748
Size: 2699 4055
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KasI G'GCGC_C
Cuts at: 2611 2713 3462 2611
Size: 102 749 5903
Fragments arranged by size:
5903 749 102
KpnI G_GTAC'C
Cuts at: 4079 4079
Size: 6754
MfeI C'AATT_G
Cuts at: 4353 4353
Size: 6754
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2798 5868 6052 1305
Size: 1493 3070 184 2007
Fragments arranged by size:
3070 2007 1493 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4932
Fragments arranged by size:
4932 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4712 5071 5230 518
Size: 648 3546 359 159 2042
Fragments arranged by size:
3546 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2447 2638 2704 2866 2900 3398 4170 4847
Size: 841 191 66 162 34 498 772 677
Cuts at: 4847 5995 6240 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 841 772 677 498 245 191
162 66 34
NaeI GCC'GGC
Cuts at: 2302 4002 2302
Size: 1700 5054
NarI GG'CG_CC
Cuts at: 2612 2714 3463 2612
Size: 102 749 5903
Fragments arranged by size:
5903 749 102
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6202
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6754
NgoMIV G'CCGG_C
Cuts at: 2300 4000 2300
Size: 1700 5054
NheI G'CTAG_C
Cuts at: 1052 3611 1052
Size: 2559 4195
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 4112 5657 1132
Size: 97 2883 1545 2229
Fragments arranged by size:
2883 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3118 3175 1348
Size: 1770 57 4927
Fragments arranged by size:
4927 1770 57
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2434 2825 1108
Size: 1326 391 5037
Fragments arranged by size:
5037 1326 391
PfoI T'CCnGG_A
Cuts at: 2818 2818
Size: 6754
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4823 4855 6336 6368 4823
Size: 32 1481 32 5209
Fragments arranged by size:
5209 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 4324 4324
Size: 6754
PspOMI G'GGCC_C
Cuts at: 3520 3520
Size: 6754
PstI C_TGCA'G
Cuts at: 830 2452 2643 2899 2908 3551 4106 830
Size: 1622 191 256 9 643 555 3478
Fragments arranged by size:
3478 1622 643 555 256 191 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 2447 2704 2866 2447
Size: 257 162 6335
Fragments arranged by size:
6335 257 162
SacI G_AGCT'C
Cuts at: 2789 2921 2789
Size: 132 6622
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SapI GCTCTTCn'nnn_
Cuts at: 2171 2171
Size: 6754
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 5002 6714 5002
Size: 1712 5042
SfcI C'TryA_G
Cuts at: 826 1045 2448 2639 2861 2895 2904 3513 3547
Size: 219 1403 191 222 34 9 609 34
Cuts at: 3547 3606 4102 5237 5918 6109 826
Size: 59 496 1135 681 191 1471
Fragments arranged by size:
1471 1403 1135 681 609 496 222 219
191 191 59 34 34 9
SfoI GGC'GCC
Cuts at: 2613 2715 3464 2613
Size: 102 749 5903
Fragments arranged by size:
5903 749 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 4081 1872
Size: 2209 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3713 3836 4858 5759 6021 820
Size: 197 926 1770 123 1022 901 262 1553
Fragments arranged by size:
1770 1553 1022 926 901 262 197 123
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 2983 152
Size: 2831 3923
SphI G_CATG'C
Cuts at: 1132 4112 1132
Size: 2980 3774
SspI AAT'ATT
Cuts at: 5 52 4678 5
Size: 47 4626 2081
Fragments arranged by size:
4626 2081 47
StuI AGG'CCT
Cuts at: 1517 2939 1517
Size: 1422 5332
StyI C'CwwG_G
Cuts at: 513 1065 2428 3256 3472 4193 513
Size: 552 1363 828 216 721 3074
Fragments arranged by size:
3074 1363 828 721 552 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2866 3188 3208 3785 3908 3936 4781
Size: 1255 1286 322 20 577 123 28 845
Cuts at: 4781 4798 4951 5136 5581 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1286 1255 845 577 445 322 185
153 123 28 20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5000 6712 96
Size: 274 80 33 51 1443 3023 1712 138
Fragments arranged by size:
3023 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2732 2764 3209 3241 6714 6746 2732
Size: 32 445 32 3473 32 2740
Fragments arranged by size:
3473 2740 445 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6754
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 4079 1870
Size: 2209 4545
XmnI GAAnn'nnTTC
Cuts at: 4883 4883
Size: 6754
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3234 4562 276
Size: 53 83 186 936 1700 1328 2468
Fragments arranged by size:
2468 1700 1328 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI